hisI

Functionⁱ

Catalytic activityⁱ

1-(5-phospho-beta-D-ribosyl)-ATP + H₂O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate.

1-(5-phospho-beta-D-ribosyl)-AMP + H₂O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide.

Pathwayⁱ: L-histidine biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:

ATP phosphoribosyltransferase (hisG)
Histidine biosynthesis bifunctional protein HisIE (hisI)
Histidine biosynthesis bifunctional protein HisIE (hisI)
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
Histidine biosynthesis bifunctional protein HisB (hisB)
Histidinol-phosphate aminotransferase (hisC)
Histidine biosynthesis bifunctional protein HisB (hisB)
Histidinol dehydrogenase (hisD)

This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
phosphoribosyl-AMP cyclohydrolase activity
Source: EcoCyc
Inferred from sequence or structural similarityⁱ
- 3018428
phosphoribosyl-ATP diphosphatase activity
Source: EcoCyc
Inferred from genetic interactionⁱ
- 7037745

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

histidine biosynthetic process
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 9733547

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Multifunctional enzyme
Biological process	Amino-acid biosynthesis, Histidine biosynthesis
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:HISTCYCLOPRATPPHOS MetaCyc:HISTCYCLOPRATPPHOS
UniPathwayⁱ	UPA00031;UER00007 UPA00031;UER00008

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Histidine biosynthesis bifunctional protein HisIE Including the following 2 domains: Phosphoribosyl-AMP cyclohydrolase (EC:3.5.4.19) Short name: PRA-CH Phosphoribosyl-ATP pyrophosphatase (EC:3.6.1.31) Short name: PRA-PH
Gene namesⁱ	Name:hisI Synonyms:hisIE Ordered Locus Names:b2026, JW2008
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10451 hisI

EcoGeneⁱ

EG10451 hisI

Subcellular locationⁱ

Cytoplasm

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000136411	1 – 203	Histidine biosynthesis bifunctional protein HisIEAdd BLAST		203

Proteomic databases

PaxDbⁱ	P06989
PRIDEⁱ	P06989

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4260421, 19 interactors
Database of interacting proteins More...DIPⁱ	DIP-9907N
IntActⁱ	P06989, 15 interactors
STRINGⁱ	316385.ECDH10B_2174

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P06989
SMRⁱ	P06989
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	1 – 114	Phosphoribosyl-AMP cyclohydrolaseAdd BLAST		114
Regionⁱ	115 – 203	Phosphoribosyl-ATP pyrophosphohydrolaseAdd BLAST		89

Sequence similaritiesⁱ

In the N-terminal section; belongs to the PRA-CH family.Curated

In the C-terminal section; belongs to the PRA-PH family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105K8F Bacteria COG0139 LUCA COG0140 LUCA
HOGENOMⁱ	HOG000277503
InParanoidⁱ	P06989
KEGG Orthology (KO) More...KOⁱ	K11755
OMAⁱ	VMACKDD
PhylomeDBⁱ	P06989

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd11534 NTP-PPase_HisIE_like, 1 hit
Gene3Dⁱ	3.10.20.400, 1 hit
HAMAPⁱ	MF_01020 HisE, 1 hit MF_01019 HisIE, 1 hit
InterProⁱ	View protein in InterPro IPR023019 His_synth_HisIE IPR008179 HisE IPR021130 PRib-ATP_PPHydrolase-like IPR002496 PRib_AMP_CycHydrolase_dom IPR038019 PRib_AMP_CycHydrolase_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01502 PRA-CH, 1 hit PF01503 PRA-PH, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD002610 PRA_CycHdrlase, 1 hit
SUPFAMⁱ	SSF141734 SSF141734, 1 hit
TIGRFAMsⁱ	TIGR03188 histidine_hisI, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P06989-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLTEQQRREL DWEKTDGLMP VIVQHAVSGE VLMLGYMNPE ALDKTLESGK 
        60         70         80         90        100
VTFFSRTKQR LWTKGETSGN FLNVVSIAPD CDNDTLLVLA NPIGPTCHKG 
       110        120        130        140        150
TSSCFGDTAH QWLFLYQLEQ LLAERKSADP ETSYTAKLYA SGTKRIAQKV 
       160        170        180        190        200
GEEGVETALA ATVHDRFELT NEASDLMYHL LVLLQDQGLD LTTVIENLRK 

RHQ

Length:203

Mass (Da):22,756

Last modified:February 1, 1995 - v3

Checksum:ⁱ34019009D82E0122

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	68	S → P in CAA31818 (PubMed:3062174).Curated	1
Sequence conflictⁱ	193	T → P (PubMed:3062174).Curated	1
Sequence conflictⁱ	193	T → P (PubMed:3018428).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱ	5	Q → L in strain: F719.	1
Natural variantⁱ	46	L → I in strain: F719.	1
Natural variantⁱ	164	H → N in strain: F719.	1
Natural variantⁱ	192 – 193	TT → GE in strain: F719.	2
Natural variantⁱ	196	E → D in strain: F719.	1
Natural variantⁱ	199 – 200	RK → KN in strain: F719.	2
Natural variantⁱ	203	Missing in strain: F719.	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X13462 Genomic DNA Translation: CAA31818.1 X03974 Genomic DNA Translation: CAA27613.1 U02072 Unassigned DNA Translation: AAA19744.1 D43637 Genomic DNA Translation: BAA07753.1 U00096 Genomic DNA Translation: AAC75087.1 AP009048 Genomic DNA Translation: BAA15858.1
PIRⁱ	JS0135 YNECHI
NCBI Reference Sequences More...RefSeqⁱ	NP_416530.1, NC_000913.3 WP_000954911.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC75087; AAC75087; b2026 BAA15858; BAA15858; BAA15858
GeneIDⁱ	946515
KEGGⁱ	ecj:JW2008 eco:b2026
PATRICⁱ	fig\|1411691.4.peg.226

Protein	Similar proteins		Species	Score	Length	Source
P06989	Histidine biosynthesis bifunctional protein HisIE		uncultured bacterium		203	UniRef100_P06989
Histidine biosynthesis bifunctional protein HisIE		uncultured actinobacterium HF0130_15N16		203
Histidine biosynthesis bifunctional protein HisIE		ECOLX		203
Histidine biosynthesis bifunctional protein HisIE		Escherichia coli 907672		203
Histidine biosynthesis bifunctional protein HisIE		ECOSM		203
+21

Protein	Similar proteins		Species	Score	Length	Source
P06989	Histidine biosynthesis bifunctional protein HisIE		SALTY		203	UniRef90_P06989
Histidine biosynthesis bifunctional protein HisIE		uncultured bacterium		203
Histidine biosynthesis bifunctional protein HisIE		uncultured actinobacterium HF0130_15N16		203
Histidine biosynthesis bifunctional protein HisIE		ECOLX		203
Histidine biosynthesis bifunctional protein HisIE		Escherichia coli 907672		203
+368

Protein	Similar proteins		Species	Score	Length	Source
P06989	Histidine biosynthesis bifunctional protein HisIE		SALTY		203	UniRef50_P06989
Histidine biosynthesis bifunctional protein HisIE		VIBVY		211
Histidine biosynthesis bifunctional protein HisIE		VIBVU		211
Histidine biosynthesis bifunctional protein HisIE		HAEIN		221
Histidine biosynthesis bifunctional protein HisIE		VIBPA		211
+2086

Entry informationⁱ

Entry nameⁱ	HIS2_ECOLI
Accessionⁱ	P06989Primary (citable) accession number: P06989 Secondary accession number(s): P78079, Q47596
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
	Last sequence update:	February 1, 1995
	Last modified:	March 28, 2018
	This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB - P06989 (HIS2_ECOLI)

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Histidine biosynthesis bifunctional protein HisIE

Select a section on the left to see content.

Functionⁱ

Catalytic activityⁱ

Pathwayⁱ: L-histidine biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Enzyme and pathway databases

Names & Taxonomyⁱ

Organism-specific databases

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Molecule processing

Proteomic databases

Interactionⁱ

Protein-protein interaction databases

Structureⁱ

3D structure databases

Family & Domainsⁱ

Region

Sequence similaritiesⁱ

Phylogenomic databases

Family and domain databases

Sequenceⁱ

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Similar proteinsⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ

UniProt is an ELIXIR core data resource

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P06989 (HIS2_ECOLI)

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Histidine biosynthesis bifunctional protein HisIE

hisI

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-histidine biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

UniProt is an ELIXIR core data resource

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Functionⁱ

Pathwayⁱ: L-histidine biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ