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UniProtKB - P06989 (HIS2_ECOLI)

Entry version 165 (02 Dec 2020)
Sequence version 3 (01 Feb 1995)
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Protein

Histidine biosynthesis bifunctional protein HisIE

Gene

hisI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-histidine biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG), ATP phosphoribosyltransferase (hisG)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI), Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI), Histidine biosynthesis bifunctional protein HisIE (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisH (hisH)
  6. Histidine biosynthesis bifunctional protein HisB (hisB), Histidine biosynthesis bifunctional protein HisB (hisB)
  7. Histidinol-phosphate aminotransferase (hisC), Histidinol-phosphate aminotransferase (hisC)
  8. Histidine biosynthesis bifunctional protein HisB (hisB), Histidine biosynthesis bifunctional protein HisB (hisB)
  9. Histidinol dehydrogenase (hisD), Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoribosyl-AMP cyclohydrolase activity Source: EcoCyc
  • phosphoribosyl-ATP diphosphatase activity Source: EcoCyc
Complete GO annotation on QuickGO ...

GO - Biological processi

  • histidine biosynthetic process Source: EcoCyc
Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Multifunctional enzyme
Biological processAmino-acid biosynthesis, Histidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:HISTCYCLOPRATPPHOS
MetaCyc:HISTCYCLOPRATPPHOS

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00031;UER00007
UPA00031;UER00008

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Histidine biosynthesis bifunctional protein HisIE
Including the following 2 domains:
Phosphoribosyl-AMP cyclohydrolase (EC:3.5.4.19)
Short name:
PRA-CH
Phosphoribosyl-ATP pyrophosphatase (EC:3.6.1.31)
Short name:
PRA-PH
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:hisI
Synonyms:hisIE
Ordered Locus Names:b2026, JW2008
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Complete GO annotation on QuickGO ...

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001364111 – 203Histidine biosynthesis bifunctional protein HisIEAdd BLAST203

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P06989

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P06989

PRoteomics IDEntifications database

More...PRIDEi		P06989

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4260421, 19 interactors

Database of interacting proteins

More...DIPi		DIP-9907N

Protein interaction database and analysis system

More...IntActi		P06989, 15 interactors

STRING: functional protein association networks

More...STRINGi		511145.b2026

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P06989

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 114Phosphoribosyl-AMP cyclohydrolaseAdd BLAST114
Regioni115 – 203Phosphoribosyl-ATP pyrophosphohydrolaseAdd BLAST89

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the N-terminal section; belongs to the PRA-CH family.Curated
In the C-terminal section; belongs to the PRA-PH family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0139, Bacteria
COG0140, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_048577_3_1_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P06989

Database for complete collections of gene phylogenies

More...PhylomeDBi		P06989

Family and domain databases

Conserved Domains Database

More...CDDi		cd11534, NTP-PPase_HisIE_like, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.10.20.400, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_01020, HisE, 1 hit
MF_01019, HisIE, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR023019, His_synth_HisIE
IPR008179, HisE
IPR021130, PRib-ATP_PPHydrolase-like
IPR002496, PRib_AMP_CycHydrolase_dom
IPR038019, PRib_AMP_CycHydrolase_sf

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01502, PRA-CH, 1 hit
PF01503, PRA-PH, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF141734, SSF141734, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR03188, histidine_hisI, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P06989-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLTEQQRREL DWEKTDGLMP VIVQHAVSGE VLMLGYMNPE ALDKTLESGK 
        60         70         80         90        100
VTFFSRTKQR LWTKGETSGN FLNVVSIAPD CDNDTLLVLA NPIGPTCHKG 
       110        120        130        140        150
TSSCFGDTAH QWLFLYQLEQ LLAERKSADP ETSYTAKLYA SGTKRIAQKV 
       160        170        180        190        200
GEEGVETALA ATVHDRFELT NEASDLMYHL LVLLQDQGLD LTTVIENLRK 

RHQ
Length:203
Mass (Da):22,756
Last modified:February 1, 1995 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i34019009D82E0122
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti68S → P in CAA31818 (PubMed:3062174).Curated1
Sequence conflicti193T → P (PubMed:3062174).Curated1
Sequence conflicti193T → P (PubMed:3018428).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural varianti5Q → L in strain: F719. 1
Natural varianti46L → I in strain: F719. 1
Natural varianti164H → N in strain: F719. 1
Natural varianti192 – 193TT → GE in strain: F719. 2
Natural varianti196E → D in strain: F719. 1
Natural varianti199 – 200RK → KN in strain: F719. 2
Natural varianti203Missing in strain: F719. 1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X13462 Genomic DNA Translation: CAA31818.1
X03974 Genomic DNA Translation: CAA27613.1
U02072 Unassigned DNA Translation: AAA19744.1
D43637 Genomic DNA Translation: BAA07753.1
U00096 Genomic DNA Translation: AAC75087.1
AP009048 Genomic DNA Translation: BAA15858.1

Protein sequence database of the Protein Information Resource

More...PIRi		JS0135, YNECHI

NCBI Reference Sequences

More...RefSeqi		NP_416530.1, NC_000913.3
WP_000954911.1, NZ_SSUR01000003.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC75087; AAC75087; b2026
BAA15858; BAA15858; BAA15858

Database of genes from NCBI RefSeq genomes

More...GeneIDi		49584196
946515

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW2008
eco:b2026

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.226

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13462 Genomic DNA Translation: CAA31818.1
X03974 Genomic DNA Translation: CAA27613.1
U02072 Unassigned DNA Translation: AAA19744.1
D43637 Genomic DNA Translation: BAA07753.1
U00096 Genomic DNA Translation: AAC75087.1
AP009048 Genomic DNA Translation: BAA15858.1
PIRiJS0135, YNECHI
RefSeqiNP_416530.1, NC_000913.3
WP_000954911.1, NZ_SSUR01000003.1

3D structure databases

SMRiP06989
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4260421, 19 interactors
DIPiDIP-9907N
IntActiP06989, 15 interactors
STRINGi511145.b2026

Proteomic databases

jPOSTiP06989
PaxDbiP06989
PRIDEiP06989

Genome annotation databases

EnsemblBacteriaiAAC75087; AAC75087; b2026
BAA15858; BAA15858; BAA15858
GeneIDi49584196
946515
KEGGiecj:JW2008
eco:b2026
PATRICifig|1411691.4.peg.226

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB0446

Phylogenomic databases

eggNOGiCOG0139, Bacteria
COG0140, Bacteria
HOGENOMiCLU_048577_3_1_6
InParanoidiP06989
PhylomeDBiP06989

Enzyme and pathway databases

UniPathwayiUPA00031;UER00007
UPA00031;UER00008
BioCyciEcoCyc:HISTCYCLOPRATPPHOS
MetaCyc:HISTCYCLOPRATPPHOS

Miscellaneous databases

Protein Ontology

More...PROi		PR:P06989

Family and domain databases

CDDicd11534, NTP-PPase_HisIE_like, 1 hit
Gene3Di3.10.20.400, 1 hit
HAMAPiMF_01020, HisE, 1 hit
MF_01019, HisIE, 1 hit
InterProiView protein in InterPro
IPR023019, His_synth_HisIE
IPR008179, HisE
IPR021130, PRib-ATP_PPHydrolase-like
IPR002496, PRib_AMP_CycHydrolase_dom
IPR038019, PRib_AMP_CycHydrolase_sf
PfamiView protein in Pfam
PF01502, PRA-CH, 1 hit
PF01503, PRA-PH, 1 hit
SUPFAMiSSF141734, SSF141734, 1 hit
TIGRFAMsiTIGR03188, histidine_hisI, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHIS2_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P06989
Secondary accession number(s): P78079, Q47596
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1995
Last modified: December 2, 2020
This is version 165 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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