UniProtKB - P06989 (HIS2_ECOLI)
Protein
Histidine biosynthesis bifunctional protein HisIE
Gene
hisI
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- EC:3.6.1.31
- 1-(5-phospho-β-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-β-D-ribosyl)-5-[(5-phospho-β-D-ribosylamino)methylideneamino]imidazole-4-carboxamideEC:3.5.4.19
: L-histidine biosynthesis Pathwayi
This protein is involved in step 2 and 3 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.Proteins known to be involved in the 9 steps of the subpathway in this organism are:
- ATP phosphoribosyltransferase (hisG), ATP phosphoribosyltransferase (hisG)
- Histidine biosynthesis bifunctional protein HisIE (hisI), Histidine biosynthesis bifunctional protein HisIE (hisI)
- Histidine biosynthesis bifunctional protein HisIE (hisI), Histidine biosynthesis bifunctional protein HisIE (hisI)
- 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
- Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisH (hisH)
- Histidine biosynthesis bifunctional protein HisB (hisB), Histidine biosynthesis bifunctional protein HisB (hisB)
- Histidinol-phosphate aminotransferase (hisC), Histidinol-phosphate aminotransferase (hisC)
- Histidine biosynthesis bifunctional protein HisB (hisB), Histidine biosynthesis bifunctional protein HisB (hisB)
- Histidinol dehydrogenase (hisD), Histidinol dehydrogenase (hisD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- phosphoribosyl-AMP cyclohydrolase activity Source: EcoCyc
- phosphoribosyl-ATP diphosphatase activity Source: EcoCyc
GO - Biological processi
- histidine biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Hydrolase, Multifunctional enzyme |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:HISTCYCLOPRATPPHOS MetaCyc:HISTCYCLOPRATPPHOS |
UniPathwayi | UPA00031;UER00007 UPA00031;UER00008 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:hisI Synonyms:hisIE Ordered Locus Names:b2026, JW2008 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000136411 | 1 – 203 | Histidine biosynthesis bifunctional protein HisIEAdd BLAST | 203 |
Proteomic databases
jPOSTi | P06989 |
PaxDbi | P06989 |
PRIDEi | P06989 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260421, 19 interactors |
DIPi | DIP-9907N |
IntActi | P06989, 15 interactors |
STRINGi | 511145.b2026 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 114 | Phosphoribosyl-AMP cyclohydrolaseAdd BLAST | 114 | |
Regioni | 115 – 203 | Phosphoribosyl-ATP pyrophosphohydrolaseAdd BLAST | 89 |
Sequence similaritiesi
In the N-terminal section; belongs to the PRA-CH family.Curated
In the C-terminal section; belongs to the PRA-PH family.Curated
Phylogenomic databases
eggNOGi | COG0139, Bacteria COG0140, Bacteria |
HOGENOMi | CLU_048577_3_1_6 |
InParanoidi | P06989 |
PhylomeDBi | P06989 |
Family and domain databases
CDDi | cd11534, NTP-PPase_HisIE_like, 1 hit |
Gene3Di | 3.10.20.400, 1 hit |
HAMAPi | MF_01020, HisE, 1 hit MF_01019, HisIE, 1 hit |
InterProi | View protein in InterPro IPR023019, His_synth_HisIE IPR008179, HisE IPR021130, PRib-ATP_PPHydrolase-like IPR002496, PRib_AMP_CycHydrolase_dom IPR038019, PRib_AMP_CycHydrolase_sf |
Pfami | View protein in Pfam PF01502, PRA-CH, 1 hit PF01503, PRA-PH, 1 hit |
SUPFAMi | SSF141734, SSF141734, 1 hit |
TIGRFAMsi | TIGR03188, histidine_hisI, 1 hit |
i Sequence
Sequence statusi: Complete.
P06989-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLTEQQRREL DWEKTDGLMP VIVQHAVSGE VLMLGYMNPE ALDKTLESGK
60 70 80 90 100
VTFFSRTKQR LWTKGETSGN FLNVVSIAPD CDNDTLLVLA NPIGPTCHKG
110 120 130 140 150
TSSCFGDTAH QWLFLYQLEQ LLAERKSADP ETSYTAKLYA SGTKRIAQKV
160 170 180 190 200
GEEGVETALA ATVHDRFELT NEASDLMYHL LVLLQDQGLD LTTVIENLRK
RHQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 68 | S → P in CAA31818 (PubMed:3062174).Curated | 1 | |
Sequence conflicti | 193 | T → P (PubMed:3062174).Curated | 1 | |
Sequence conflicti | 193 | T → P (PubMed:3018428).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 5 | Q → L in strain: F719. | 1 | |
Natural varianti | 46 | L → I in strain: F719. | 1 | |
Natural varianti | 164 | H → N in strain: F719. | 1 | |
Natural varianti | 192 – 193 | TT → GE in strain: F719. | 2 | |
Natural varianti | 196 | E → D in strain: F719. | 1 | |
Natural varianti | 199 – 200 | RK → KN in strain: F719. | 2 | |
Natural varianti | 203 | Missing in strain: F719. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13462 Genomic DNA Translation: CAA31818.1 X03974 Genomic DNA Translation: CAA27613.1 U02072 Unassigned DNA Translation: AAA19744.1 D43637 Genomic DNA Translation: BAA07753.1 U00096 Genomic DNA Translation: AAC75087.1 AP009048 Genomic DNA Translation: BAA15858.1 |
PIRi | JS0135, YNECHI |
RefSeqi | NP_416530.1, NC_000913.3 WP_000954911.1, NZ_SSUR01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC75087; AAC75087; b2026 BAA15858; BAA15858; BAA15858 |
GeneIDi | 49584196 946515 |
KEGGi | ecj:JW2008 eco:b2026 |
PATRICi | fig|1411691.4.peg.226 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13462 Genomic DNA Translation: CAA31818.1 X03974 Genomic DNA Translation: CAA27613.1 U02072 Unassigned DNA Translation: AAA19744.1 D43637 Genomic DNA Translation: BAA07753.1 U00096 Genomic DNA Translation: AAC75087.1 AP009048 Genomic DNA Translation: BAA15858.1 |
PIRi | JS0135, YNECHI |
RefSeqi | NP_416530.1, NC_000913.3 WP_000954911.1, NZ_SSUR01000003.1 |
3D structure databases
SMRi | P06989 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260421, 19 interactors |
DIPi | DIP-9907N |
IntActi | P06989, 15 interactors |
STRINGi | 511145.b2026 |
Proteomic databases
jPOSTi | P06989 |
PaxDbi | P06989 |
PRIDEi | P06989 |
Genome annotation databases
EnsemblBacteriai | AAC75087; AAC75087; b2026 BAA15858; BAA15858; BAA15858 |
GeneIDi | 49584196 946515 |
KEGGi | ecj:JW2008 eco:b2026 |
PATRICi | fig|1411691.4.peg.226 |
Organism-specific databases
EchoBASEi | EB0446 |
Phylogenomic databases
eggNOGi | COG0139, Bacteria COG0140, Bacteria |
HOGENOMi | CLU_048577_3_1_6 |
InParanoidi | P06989 |
PhylomeDBi | P06989 |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00007 UPA00031;UER00008 |
BioCyci | EcoCyc:HISTCYCLOPRATPPHOS MetaCyc:HISTCYCLOPRATPPHOS |
Miscellaneous databases
PROi | PR:P06989 |
Family and domain databases
CDDi | cd11534, NTP-PPase_HisIE_like, 1 hit |
Gene3Di | 3.10.20.400, 1 hit |
HAMAPi | MF_01020, HisE, 1 hit MF_01019, HisIE, 1 hit |
InterProi | View protein in InterPro IPR023019, His_synth_HisIE IPR008179, HisE IPR021130, PRib-ATP_PPHydrolase-like IPR002496, PRib_AMP_CycHydrolase_dom IPR038019, PRib_AMP_CycHydrolase_sf |
Pfami | View protein in Pfam PF01502, PRA-CH, 1 hit PF01503, PRA-PH, 1 hit |
SUPFAMi | SSF141734, SSF141734, 1 hit |
TIGRFAMsi | TIGR03188, histidine_hisI, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HIS2_ECOLI | |
Accessioni | P06989Primary (citable) accession number: P06989 Secondary accession number(s): P78079, Q47596 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | February 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families