UniProtKB - P06986 (HIS8_ECOLI)
Protein
Histidinol-phosphate aminotransferase
Gene
hisC
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- 2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate1 PublicationEC:2.6.1.91 Publication
Cofactori
pyridoxal 5'-phosphate2 Publications
: L-histidine biosynthesis Pathwayi
This protein is involved in step 7 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.1 PublicationProteins known to be involved in the 9 steps of the subpathway in this organism are:
- ATP phosphoribosyltransferase (hisG), ATP phosphoribosyltransferase (hisG)
- Histidine biosynthesis bifunctional protein HisIE (hisI), Histidine biosynthesis bifunctional protein HisIE (hisI)
- Histidine biosynthesis bifunctional protein HisIE (hisI), Histidine biosynthesis bifunctional protein HisIE (hisI)
- 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
- Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisH (hisH)
- Histidine biosynthesis bifunctional protein HisB (hisB), Histidine biosynthesis bifunctional protein HisB (hisB)
- Histidinol-phosphate aminotransferase (hisC), Histidinol-phosphate aminotransferase (hisC)
- Histidine biosynthesis bifunctional protein HisB (hisB), Histidine biosynthesis bifunctional protein HisB (hisB)
- Histidinol dehydrogenase (hisD), Histidinol dehydrogenase (hisD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- histidinol-phosphate transaminase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- histidine biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:HISTPHOSTRANS-MONOMER MetaCyc:HISTPHOSTRANS-MONOMER |
BRENDAi | 2.6.1.9, 2026 |
UniPathwayi | UPA00031;UER00012 |
Names & Taxonomyi
Protein namesi | Recommended name: Histidinol-phosphate aminotransferase (EC:2.6.1.9)Alternative name(s): Imidazole acetol-phosphate transaminase Short name: HPAT Short name: HspAT |
Gene namesi | Name:hisC Ordered Locus Names:b2021, JW2003 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Chemistry databases
DrugBanki | DB03997, L-histidinol phosphate DB02142, Pyridoxamine-5'-Phosphate DB01813, Pyridoxyl-Glutamic Acid-5'-Monophosphate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000153360 | 1 – 356 | Histidinol-phosphate aminotransferaseAdd BLAST | 356 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 214 | N6-(pyridoxal phosphate)lysine | 1 |
Proteomic databases
jPOSTi | P06986 |
PaxDbi | P06986 |
PRIDEi | P06986 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260404, 25 interactors |
DIPi | DIP-9902N |
IntActi | P06986, 5 interactors |
STRINGi | 511145.b2021 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P06986 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06986 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.Curated
Phylogenomic databases
eggNOGi | COG0079, Bacteria |
HOGENOMi | CLU_017584_3_1_6 |
InParanoidi | P06986 |
PhylomeDBi | P06986 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_01023, HisC_aminotrans_2, 1 hit |
InterProi | View protein in InterPro IPR001917, Aminotrans_II_pyridoxalP_BS IPR004839, Aminotransferase_I/II IPR005861, HisP_aminotrans IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR01141, hisC, 1 hit |
PROSITEi | View protein in PROSITE PS00599, AA_TRANSFER_CLASS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P06986-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTVTITDLA RENVRNLTPY QSARRLGGNG DVWLNANEYP TAVEFQLTQQ
60 70 80 90 100
TLNRYPECQP KAVIENYAQY AGVKPEQVLV SRGADEGIEL LIRAFCEPGK
110 120 130 140 150
DAILYCPPTY GMYSVSAETI GVECRTVPTL DNWQLDLQGI SDKLDGVKVV
160 170 180 190 200
YVCSPNNPTG QLINPQDFRT LLELTRGKAI VVADEAYIEF CPQASLAGWL
210 220 230 240 250
AEYPHLAILR TLSKAFALAG LRCGFTLANE EVINLLMKVI APYPLSTPVA
260 270 280 290 300
DIAAQALSPQ GIVAMRERVA QIIAEREYLI AALKEIPCVE QVFDSETNYI
310 320 330 340 350
LARFKASSAV FKSLWDQGII LRDQNKQPSL SGCLRITVGT REESQRVIDA
LRAEQV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 130 | L → P (PubMed:3062174).Curated | 1 | |
Sequence conflicti | 130 | L → P (PubMed:2999081).Curated | 1 | |
Sequence conflicti | 149 | V → A (PubMed:3062174).Curated | 1 | |
Sequence conflicti | 149 | V → A (PubMed:2999081).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03416 Genomic DNA Translation: CAA27150.1 X13462 Genomic DNA Translation: CAA31813.1 U02071 Unassigned DNA Translation: AAA19743.1 U00096 Genomic DNA Translation: AAC75082.1 AP009048 Genomic DNA Translation: BAA15852.1 |
PIRi | D64967, XNECHC |
RefSeqi | NP_416525.1, NC_000913.3 WP_000108941.1, NZ_SSTT01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75082; AAC75082; b2021 BAA15852; BAA15852; BAA15852 |
GeneIDi | 946551 |
KEGGi | ecj:JW2003 eco:b2021 |
PATRICi | fig|1411691.4.peg.231 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03416 Genomic DNA Translation: CAA27150.1 X13462 Genomic DNA Translation: CAA31813.1 U02071 Unassigned DNA Translation: AAA19743.1 U00096 Genomic DNA Translation: AAC75082.1 AP009048 Genomic DNA Translation: BAA15852.1 |
PIRi | D64967, XNECHC |
RefSeqi | NP_416525.1, NC_000913.3 WP_000108941.1, NZ_SSTT01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FG3 | X-ray | 2.20 | A | 1-356 | [»] | |
1FG7 | X-ray | 1.50 | A | 1-356 | [»] | |
1GEW | X-ray | 2.00 | A | 1-356 | [»] | |
1GEX | X-ray | 2.20 | A | 1-356 | [»] | |
1GEY | X-ray | 2.30 | A | 1-356 | [»] | |
1IJI | X-ray | 2.20 | A | 1-356 | [»] | |
SMRi | P06986 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260404, 25 interactors |
DIPi | DIP-9902N |
IntActi | P06986, 5 interactors |
STRINGi | 511145.b2021 |
Chemistry databases
DrugBanki | DB03997, L-histidinol phosphate DB02142, Pyridoxamine-5'-Phosphate DB01813, Pyridoxyl-Glutamic Acid-5'-Monophosphate |
Proteomic databases
jPOSTi | P06986 |
PaxDbi | P06986 |
PRIDEi | P06986 |
Genome annotation databases
EnsemblBacteriai | AAC75082; AAC75082; b2021 BAA15852; BAA15852; BAA15852 |
GeneIDi | 946551 |
KEGGi | ecj:JW2003 eco:b2021 |
PATRICi | fig|1411691.4.peg.231 |
Organism-specific databases
EchoBASEi | EB0441 |
Phylogenomic databases
eggNOGi | COG0079, Bacteria |
HOGENOMi | CLU_017584_3_1_6 |
InParanoidi | P06986 |
PhylomeDBi | P06986 |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00012 |
BioCyci | EcoCyc:HISTPHOSTRANS-MONOMER MetaCyc:HISTPHOSTRANS-MONOMER |
BRENDAi | 2.6.1.9, 2026 |
Miscellaneous databases
EvolutionaryTracei | P06986 |
PROi | PR:P06986 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_01023, HisC_aminotrans_2, 1 hit |
InterProi | View protein in InterPro IPR001917, Aminotrans_II_pyridoxalP_BS IPR004839, Aminotransferase_I/II IPR005861, HisP_aminotrans IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR01141, hisC, 1 hit |
PROSITEi | View protein in PROSITE PS00599, AA_TRANSFER_CLASS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HIS8_ECOLI | |
Accessioni | P06986Primary (citable) accession number: P06986 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | February 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families