UniProtKB - P06983 (HEM3_ECOLI)
Protein
Porphobilinogen deaminase
Gene
hemC
Organism
Escherichia coli (strain K12)
Status
Functioni
Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.1 Publication
Miscellaneous
Arginine residues that are closely associated with one another might be involved in substrate binding.
The porphobilinogen subunits are added to the dipyrromethane group.
Catalytic activityi
- EC:2.5.1.61
Cofactori
dipyrromethaneNote: Binds 1 dipyrromethane group covalently.
: protoporphyrin-IX biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Delta-aminolevulinic acid dehydratase (hemB), Delta-aminolevulinic acid dehydratase (hemB)
- Porphobilinogen deaminase (hemC), Porphobilinogen deaminase (hemC)
- Uroporphyrinogen-III synthase (hemD), Uroporphyrinogen-III synthase (hemD)
- Uroporphyrinogen decarboxylase (hemE), Uroporphyrinogen decarboxylase (hemE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.
GO - Molecular functioni
- hydroxymethylbilane synthase activity Source: EcoCyc
GO - Biological processi
- heme biosynthetic process Source: EcoliWiki
- peptidyl-pyrromethane cofactor linkage Source: EcoliWiki
- protoporphyrinogen IX biosynthetic process Source: EcoCyc
- tetrapyrrole biosynthetic process Source: EcoliWiki
Keywordsi
Molecular function | Transferase |
Biological process | Porphyrin biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:OHMETHYLBILANESYN-MONOMER MetaCyc:OHMETHYLBILANESYN-MONOMER |
UniPathwayi | UPA00251;UER00319 |
Names & Taxonomyi
Protein namesi | Recommended name: Porphobilinogen deaminase (EC:2.5.1.61)Short name: PBG Alternative name(s): Hydroxymethylbilane synthase Short name: HMBS Pre-uroporphyrinogen synthase |
Gene namesi | Name:hemC Synonyms:popE Ordered Locus Names:b3805, JW5932 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 7 | R → L: No loss of activity. | 1 | |
Mutagenesisi | 11 | R → L: Loss of activity. | 1 | |
Mutagenesisi | 55 | K → Q: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 59 | K → Q: 25-fold decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 101 | R → L: No loss of activity. | 1 | |
Mutagenesisi | 131 | R → L: Complete loss of activity. | 1 | |
Mutagenesisi | 132 | R → L: Complete loss of activity. | 1 | |
Mutagenesisi | 155 | R → L: Loss of activity. | 1 | |
Mutagenesisi | 176 | R → L: Loss of activity. | 1 |
Chemistry databases
DrugBanki | DB04517, Dipyrromethane Cofactor |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000142934 | 1 – 313 | Porphobilinogen deaminaseAdd BLAST | 313 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 242 | S-(dipyrrolylmethanemethyl)cysteine | 1 |
Proteomic databases
jPOSTi | P06983 |
PaxDbi | P06983 |
PRIDEi | P06983 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4259657, 9 interactors |
DIPi | DIP-9879N |
IntActi | P06983, 1 interactor |
STRINGi | 511145.b3805 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P06983 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06983 |
Family & Domainsi
Sequence similaritiesi
Belongs to the HMBS family.Curated
Phylogenomic databases
eggNOGi | COG0181, Bacteria |
HOGENOMi | CLU_019704_0_2_6 |
InParanoidi | P06983 |
PhylomeDBi | P06983 |
Family and domain databases
Gene3Di | 3.30.160.40, 1 hit |
HAMAPi | MF_00260, Porphobil_deam, 1 hit |
InterProi | View protein in InterPro IPR000860, HemC IPR022419, Porphobilin_deaminase_cofac_BS IPR022417, Porphobilin_deaminase_N IPR022418, Porphobilinogen_deaminase_C IPR036803, Porphobilinogen_deaminase_C_sf |
PANTHERi | PTHR11557, PTHR11557, 1 hit |
Pfami | View protein in Pfam PF01379, Porphobil_deam, 1 hit PF03900, Porphobil_deamC, 1 hit |
PIRSFi | PIRSF001438, 4pyrrol_synth_OHMeBilane_synth, 1 hit |
PRINTSi | PR00151, PORPHBDMNASE |
SUPFAMi | SSF54782, SSF54782, 1 hit |
TIGRFAMsi | TIGR00212, hemC, 1 hit |
PROSITEi | View protein in PROSITE PS00533, PORPHOBILINOGEN_DEAM, 1 hit |
i Sequence
Sequence statusi: Complete.
P06983-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLDNVLRIAT RQSPLALWQA HYVKDKLMAS HPGLVVELVP MVTRGDVILD
60 70 80 90 100
TPLAKVGGKG LFVKELEVAL LENRADIAVH SMKDVPVEFP QGLGLVTICE
110 120 130 140 150
REDPRDAFVS NNYDSLDALP AGSIVGTSSL RRQCQLAERR PDLIIRSLRG
160 170 180 190 200
NVGTRLSKLD NGEYDAIILA VAGLKRLGLE SRIRAALPPE ISLPAVGQGA
210 220 230 240 250
VGIECRLDDS RTRELLAALN HHETALRVTA ERAMNTRLEG GCQVPIGSYA
260 270 280 290 300
ELIDGEIWLR ALVGAPDGSQ IIRGERRGAP QDAEQMGISL AEELLNNGAR
310
EILAEVYNGD APA
Sequence cautioni
The sequence AAA67601 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 137 | A → G in AAA67601 (PubMed:1379743).Curated | 1 | |
Sequence conflicti | 186 | A → G in AAA67601 (PubMed:1379743).Curated | 1 | |
Sequence conflicti | 241 | G → A in CAA27813 (PubMed:3529035).Curated | 1 | |
Sequence conflicti | 261 | A → G in CAA27813 (PubMed:3529035).Curated | 1 | |
Sequence conflicti | 265 | A → R in AAA67601 (PubMed:1379743).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04242 Genomic DNA Translation: CAA27813.1 X12614 Genomic DNA Translation: CAA31132.1 M87049 Genomic DNA Translation: AAA67601.1 Different initiation. U00096 Genomic DNA Translation: AAT48218.1 AP009048 Genomic DNA Translation: BAE77496.1 X66782 Genomic DNA Translation: CAA47279.1 |
PIRi | F65184, IBEC |
RefSeqi | WP_001338644.1, NZ_SSZK01000025.1 YP_026260.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48218; AAT48218; b3805 BAE77496; BAE77496; BAE77496 |
GeneIDi | 57730178 947759 |
KEGGi | ecj:JW5932 eco:b3805 |
PATRICi | fig|511145.12.peg.3920 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04242 Genomic DNA Translation: CAA27813.1 X12614 Genomic DNA Translation: CAA31132.1 M87049 Genomic DNA Translation: AAA67601.1 Different initiation. U00096 Genomic DNA Translation: AAT48218.1 AP009048 Genomic DNA Translation: BAE77496.1 X66782 Genomic DNA Translation: CAA47279.1 |
PIRi | F65184, IBEC |
RefSeqi | WP_001338644.1, NZ_SSZK01000025.1 YP_026260.1, NC_000913.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AH5 | X-ray | 2.40 | A | 1-313 | [»] | |
1GTK | X-ray | 1.66 | A | 1-313 | [»] | |
1PDA | X-ray | 1.76 | A | 1-313 | [»] | |
1YPN | X-ray | 2.30 | A | 1-313 | [»] | |
2YPN | X-ray | 2.30 | A | 1-313 | [»] | |
SMRi | P06983 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259657, 9 interactors |
DIPi | DIP-9879N |
IntActi | P06983, 1 interactor |
STRINGi | 511145.b3805 |
Chemistry databases
DrugBanki | DB04517, Dipyrromethane Cofactor |
Proteomic databases
jPOSTi | P06983 |
PaxDbi | P06983 |
PRIDEi | P06983 |
Genome annotation databases
EnsemblBacteriai | AAT48218; AAT48218; b3805 BAE77496; BAE77496; BAE77496 |
GeneIDi | 57730178 947759 |
KEGGi | ecj:JW5932 eco:b3805 |
PATRICi | fig|511145.12.peg.3920 |
Organism-specific databases
EchoBASEi | EB0424 |
Phylogenomic databases
eggNOGi | COG0181, Bacteria |
HOGENOMi | CLU_019704_0_2_6 |
InParanoidi | P06983 |
PhylomeDBi | P06983 |
Enzyme and pathway databases
UniPathwayi | UPA00251;UER00319 |
BioCyci | EcoCyc:OHMETHYLBILANESYN-MONOMER MetaCyc:OHMETHYLBILANESYN-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P06983 |
PROi | PR:P06983 |
Family and domain databases
Gene3Di | 3.30.160.40, 1 hit |
HAMAPi | MF_00260, Porphobil_deam, 1 hit |
InterProi | View protein in InterPro IPR000860, HemC IPR022419, Porphobilin_deaminase_cofac_BS IPR022417, Porphobilin_deaminase_N IPR022418, Porphobilinogen_deaminase_C IPR036803, Porphobilinogen_deaminase_C_sf |
PANTHERi | PTHR11557, PTHR11557, 1 hit |
Pfami | View protein in Pfam PF01379, Porphobil_deam, 1 hit PF03900, Porphobil_deamC, 1 hit |
PIRSFi | PIRSF001438, 4pyrrol_synth_OHMeBilane_synth, 1 hit |
PRINTSi | PR00151, PORPHBDMNASE |
SUPFAMi | SSF54782, SSF54782, 1 hit |
TIGRFAMsi | TIGR00212, hemC, 1 hit |
PROSITEi | View protein in PROSITE PS00533, PORPHOBILINOGEN_DEAM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HEM3_ECOLI | |
Accessioni | P06983Primary (citable) accession number: P06983 Secondary accession number(s): P78125, Q2M8B0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 198 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families