UniProtKB - P06974 (FLIM_ECOLI)
Protein
Flagellar motor switch protein FliM
Gene
fliM
Organism
Escherichia coli (strain K12)
Status
Functioni
FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.
GO - Molecular functioni
- motor activity Source: InterPro
GO - Biological processi
- bacterial-type flagellum-dependent cell motility Source: InterPro
- positive chemotaxis Source: GO_Central
Keywordsi
Biological process | Chemotaxis, Flagellar rotation |
Enzyme and pathway databases
BioCyci | EcoCyc:FLIM-FLAGELLAR-C-RING-SWITCH |
Names & Taxonomyi
Protein namesi | Recommended name: Flagellar motor switch protein FliM |
Gene namesi | Name:fliM Synonyms:cheC2, fla AII, fla QII Ordered Locus Names:b1945, JW1929 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- bacterial-type flagellum basal body Source: UniProtKB-SubCell
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Bacterial flagellum, Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 153 | V → D: Decreases binding to YcgR. 1 Publication | 1 | |
Mutagenesisi | 155 | N → E: Decreases binding to YcgR. 1 Publication | 1 | |
Mutagenesisi | 156 | R → D: Obviates binding to YcgR. 1 Publication | 1 | |
Mutagenesisi | 160 | L → E: Obviates binding to YcgR. 1 Publication | 1 | |
Mutagenesisi | 167 | D → R: Decreases binding to YcgR. 1 Publication | 1 | |
Mutagenesisi | 176 | E → R: Decreases binding to YcgR. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000180928 | 1 – 334 | Flagellar motor switch protein FliMAdd BLAST | 334 |
Proteomic databases
PaxDbi | P06974 |
PRIDEi | P06974 |
Interactioni
Subunit structurei
Interacts with flagellar brake protein YcgR.
1 PublicationBinary interactionsi
Hide detailsP06974
With | #Exp. | IntAct |
---|---|---|
fliG [P0ABZ1] | 16 | EBI-560439,EBI-1126524 |
fliN [P15070] | 7 | EBI-560439,EBI-2011987 |
ycgR [P76010] | 3 | EBI-560439,EBI-554507 |
Protein-protein interaction databases
BioGRIDi | 4261570, 21 interactors 850797, 5 interactors |
ComplexPortali | CPX-1082, Flagellar Motor Switch Complex, CW variant CPX-1085, Flagellar Motor Switch Complex, CCW variant |
DIPi | DIP-1100N |
IntActi | P06974, 18 interactors |
MINTi | P06974 |
STRINGi | 511145.b1945 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P06974 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06974 |
Family & Domainsi
Sequence similaritiesi
Belongs to the FliM family.Curated
Phylogenomic databases
eggNOGi | COG1868, Bacteria |
HOGENOMi | CLU_052646_1_2_6 |
InParanoidi | P06974 |
PhylomeDBi | P06974 |
Family and domain databases
Gene3Di | 3.40.1550.10, 1 hit |
InterProi | View protein in InterPro IPR028976, CheC-like_sf IPR001689, Flag_FliM IPR001543, SpoA IPR036429, SpoA-like_sf |
Pfami | View protein in Pfam PF02154, FliM, 1 hit PF01052, FliMN_C, 1 hit |
PIRSFi | PIRSF002888, FliM, 1 hit |
PRINTSi | PR00955, FLGMOTORFLIM |
SUPFAMi | SSF101801, SSF101801, 1 hit SSF103039, SSF103039, 1 hit |
TIGRFAMsi | TIGR01397, fliM_switch, 1 hit |
i Sequence
Sequence statusi: Complete.
P06974-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGDSILSQAE IDALLNGDSE VKDEPTASVS GESDIRPYDP NTQRRVVRER
60 70 80 90 100
LQALEIINER FARHFRMGLF NLLRRSPDIT VGAIRIQPYH EFARNLPVPT
110 120 130 140 150
NLNLIHLKPL RGTGLVVFSP SLVFIAVDNL FGGDGRFPTK VEGREFTHTE
160 170 180 190 200
QRVINRMLKL ALEGYSDAWK AINPLEVEYV RSEMQVKFTN ITTSPNDIVV
210 220 230 240 250
NTPFHVEIGN LTGEFNICLP FSMIEPLREL LVNPPLENSR NEDQNWRDNL
260 270 280 290 300
VRQVQHSQLE LVANFADISL RLSQILKLNP GDVLPIEKPD RIIAHVDGVP
310 320 330
VLTSQYGTLN GQYALRIEHL INPILNSLNE EQPK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12784 Genomic DNA Translation: AAA23786.1 U00096 Genomic DNA Translation: AAC75012.1 AP009048 Genomic DNA Translation: BAA15770.1 M26294 Unassigned DNA No translation available. |
PIRi | B29842, XMECF2 |
RefSeqi | NP_416455.1, NC_000913.3 WP_001350520.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75012; AAC75012; b1945 BAA15770; BAA15770; BAA15770 |
GeneIDi | 946442 |
KEGGi | ecj:JW1929 eco:b1945 |
PATRICi | fig|1411691.4.peg.306 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12784 Genomic DNA Translation: AAA23786.1 U00096 Genomic DNA Translation: AAC75012.1 AP009048 Genomic DNA Translation: BAA15770.1 M26294 Unassigned DNA No translation available. |
PIRi | B29842, XMECF2 |
RefSeqi | NP_416455.1, NC_000913.3 WP_001350520.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1F4V | X-ray | 2.22 | D/E/F | 1-16 | [»] | |
1U8T | X-ray | 1.50 | E/F | 1-16 | [»] | |
2B1J | X-ray | 2.40 | C/D | 1-16 | [»] | |
SMRi | P06974 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261570, 21 interactors 850797, 5 interactors |
ComplexPortali | CPX-1082, Flagellar Motor Switch Complex, CW variant CPX-1085, Flagellar Motor Switch Complex, CCW variant |
DIPi | DIP-1100N |
IntActi | P06974, 18 interactors |
MINTi | P06974 |
STRINGi | 511145.b1945 |
Proteomic databases
PaxDbi | P06974 |
PRIDEi | P06974 |
Genome annotation databases
EnsemblBacteriai | AAC75012; AAC75012; b1945 BAA15770; BAA15770; BAA15770 |
GeneIDi | 946442 |
KEGGi | ecj:JW1929 eco:b1945 |
PATRICi | fig|1411691.4.peg.306 |
Organism-specific databases
EchoBASEi | EB0319 |
Phylogenomic databases
eggNOGi | COG1868, Bacteria |
HOGENOMi | CLU_052646_1_2_6 |
InParanoidi | P06974 |
PhylomeDBi | P06974 |
Enzyme and pathway databases
BioCyci | EcoCyc:FLIM-FLAGELLAR-C-RING-SWITCH |
Miscellaneous databases
EvolutionaryTracei | P06974 |
PROi | PR:P06974 |
Family and domain databases
Gene3Di | 3.40.1550.10, 1 hit |
InterProi | View protein in InterPro IPR028976, CheC-like_sf IPR001689, Flag_FliM IPR001543, SpoA IPR036429, SpoA-like_sf |
Pfami | View protein in Pfam PF02154, FliM, 1 hit PF01052, FliMN_C, 1 hit |
PIRSFi | PIRSF002888, FliM, 1 hit |
PRINTSi | PR00955, FLGMOTORFLIM |
SUPFAMi | SSF101801, SSF101801, 1 hit SSF103039, SSF103039, 1 hit |
TIGRFAMsi | TIGR01397, fliM_switch, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FLIM_ECOLI | |
Accessioni | P06974Primary (citable) accession number: P06974 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families