UniProtKB - P06971 (FHUA_ECOLI)
Protein
Ferrichrome outer membrane transporter/phage receptor
Gene
fhuA
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the uptake of iron in complex with ferrichrome, a hydroxamate-type siderophore. Binds and transports ferrichrome-iron across the outer membrane (PubMed:1089064, PubMed:2066336). In addition to its role in ferrichrome-iron transport, transports the antibiotic albomycin, which is a structural analog of ferrichrome, and acts as a receptor for colicin M, microcin J25 and bacteriophages T1, T5, phi80 and UC-1 (PubMed:1089064, PubMed:353030, PubMed:2066336, PubMed:8617231). The energy source, which is required for all FhuA functions except infection by phage T5, is provided by the inner membrane TonB system (PubMed:353030, PubMed:9353297, PubMed:12427941).6 Publications
Activity regulationi
Binding of ferrichrome or colicin M enhances the interaction between FhuA and TonB (PubMed:9353297). TonB activates FhuA through interaction with the beta-barrel (PubMed:12427941).2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 114 | Ferrichrome3 Publications | 1 | |
Binding sitei | 133 | Ferrichrome3 Publications | 1 | |
Binding sitei | 424 | Ferrichrome1 Publication | 1 | |
Binding sitei | 735 | Ferrichrome1 Publication | 1 |
GO - Molecular functioni
- iron ion binding Source: EcoliWiki
- protein domain specific binding Source: CAFA
- siderophore uptake transmembrane transporter activity Source: GO_Central
- signaling receptor activity Source: InterPro
- toxic substance binding Source: EcoliWiki
- virion binding Source: EcoliWiki
GO - Biological processi
- iron ion homeostasis Source: UniProtKB-KW
Keywordsi
Molecular function | Receptor |
Biological process | Ion transport, Iron transport, Transport |
Ligand | Iron |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10302-MONOMER MetaCyc:EG10302-MONOMER |
Protein family/group databases
TCDBi | 1.B.14.1.2, the outer membrane receptor (omr) family |
Names & Taxonomyi
Protein namesi | Recommended name: Ferrichrome outer membrane transporter/phage receptorCuratedAlternative name(s): Ferric hydroxamate receptorCurated Ferric hydroxamate uptakeCurated Ferrichrome-iron receptor1 Publication |
Gene namesi | Name:fhuA1 Publication Synonyms:tonA1 Publication Ordered Locus Names:b0150, JW0146 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Cell outer membrane 4 Publications; Multi-pass membrane protein 2 Publications
Other locations
- cell outer membrane Source: EcoCyc
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 34 – 192 | PeriplasmicAdd BLAST | 159 | |
Transmembranei | 193 – 201 | Beta stranded | 9 | |
Topological domaini | 202 – 206 | Extracellular | 5 | |
Transmembranei | 207 – 215 | Beta stranded | 9 | |
Topological domaini | 216 – 222 | Periplasmic | 7 | |
Transmembranei | 223 – 231 | Beta stranded | 9 | |
Topological domaini | 232 – 245 | ExtracellularAdd BLAST | 14 | |
Transmembranei | 246 – 255 | Beta stranded | 10 | |
Topological domaini | 256 – 259 | Periplasmic | 4 | |
Transmembranei | 260 – 268 | Beta stranded | 9 | |
Topological domaini | 269 – 312 | ExtracellularAdd BLAST | 44 | |
Transmembranei | 313 – 321 | Beta stranded | 9 | |
Topological domaini | 322 – 326 | Periplasmic | 5 | |
Transmembranei | 327 – 335 | Beta stranded | 9 | |
Topological domaini | 336 – 387 | ExtracellularAdd BLAST | 52 | |
Transmembranei | 388 – 396 | Beta stranded | 9 | |
Topological domaini | 397 – 404 | Periplasmic | 8 | |
Transmembranei | 405 – 413 | Beta stranded | 9 | |
Topological domaini | 414 – 464 | ExtracellularAdd BLAST | 51 | |
Transmembranei | 465 – 473 | Beta stranded | 9 | |
Topological domaini | 474 – 477 | Periplasmic | 4 | |
Transmembranei | 478 – 486 | Beta stranded | 9 | |
Topological domaini | 487 – 508 | ExtracellularAdd BLAST | 22 | |
Transmembranei | 509 – 517 | Beta stranded | 9 | |
Topological domaini | 518 – 522 | Periplasmic | 5 | |
Transmembranei | 523 – 531 | Beta stranded | 9 | |
Topological domaini | 532 – 551 | ExtracellularAdd BLAST | 20 | |
Transmembranei | 552 – 560 | Beta stranded | 9 | |
Topological domaini | 561 – 565 | Periplasmic | 5 | |
Transmembranei | 566 – 574 | Beta stranded | 9 | |
Topological domaini | 575 – 601 | ExtracellularAdd BLAST | 27 | |
Transmembranei | 602 – 610 | Beta stranded | 9 | |
Topological domaini | 611 – 613 | Periplasmic | 3 | |
Transmembranei | 614 – 622 | Beta stranded | 9 | |
Topological domaini | 623 – 645 | ExtracellularAdd BLAST | 23 | |
Transmembranei | 646 – 654 | Beta stranded | 9 | |
Topological domaini | 655 – 661 | Periplasmic | 7 | |
Transmembranei | 662 – 670 | Beta stranded | 9 | |
Topological domaini | 671 – 689 | ExtracellularAdd BLAST | 19 | |
Transmembranei | 690 – 698 | Beta stranded | 9 | |
Topological domaini | 699 – 705 | Periplasmic | 7 | |
Transmembranei | 706 – 714 | Beta stranded | 9 | |
Topological domaini | 715 – 737 | ExtracellularAdd BLAST | 23 | |
Transmembranei | 738 – 746 | Beta stranded | 9 | |
Topological domaini | 747 | Periplasmic | 1 |
Keywords - Cellular componenti
Cell outer membrane, MembranePathology & Biotechi
Chemistry databases
DrugBanki | DB02767, (R)-3-hydroxytetradecanoic acid DB02907, 2-Amino-Vinyl-Phosphate DB01814, 2-Tridecanoyloxy-Pentadecanoic Acid DB03548, 3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid DB02700, 3-Deoxy-D-Glucosamine DB04039, 3-Oxo-Pentadecanoic Acid DB02379, Beta-D-Glucose DB02613, Capric dimethyl amine oxide DB04220, CGP 4832 DB02724, Delta-2-Albomycin A1 DB03574, Ferricrocin-iron DB03111, Glucosamine 1-Phosphate DB02865, Glucosamine 4-Phosphate DB04526, L-Glycero-D-Manno-Heptopyranose DB03017, Lauric acid DB08231, Myristic acid DB02415, N-Octyl-2-Hydroxyethyl Sulfoxide DB02626, Phenylferricrocin-iron |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 33 | 1 PublicationAdd BLAST | 33 | |
ChainiPRO_0000034748 | 34 – 747 | Ferrichrome outer membrane transporter/phage receptorAdd BLAST | 714 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 351 ↔ 362 | |||
Disulfide bondi | 725 ↔ 731 |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | P06971 |
PRIDEi | P06971 |
PTM databases
CarbonylDBi | P06971 |
Expressioni
Inductioni
Induced 1.6-fold by hydroxyurea.1 Publication
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsP06971
With | #Exp. | IntAct |
---|---|---|
tonB [P02929] | 4 | EBI-1116714,EBI-6399993 |
mcjA [Q9X2V7] from Escherichia coli. | 2 | EBI-1116714,EBI-16100378 |
GO - Molecular functioni
- protein domain specific binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 4259745, 139 interactors |
ComplexPortali | CPX-2843, Ferrichrome outer membrane transporter complex |
DIPi | DIP-9602N |
IntActi | P06971, 7 interactors |
STRINGi | 511145.b0150 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
PCDDBi | P06971 |
SMRi | P06971 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06971 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 148 – 149 | Ferrichrome binding3 Publications | 2 | |
Regioni | 277 – 279 | Ferrichrome binding3 Publications | 3 | |
Regioni | 346 – 348 | Ferrichrome binding3 Publications | 3 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 40 – 47 | TonB box | 8 | |
Motifi | 730 – 747 | TonB C-terminal boxAdd BLAST | 18 |
Domaini
Has two distinct conformations in the presence and absence of ferrichrome. The globular N-terminal domain acts a plug that closes the channel formed by the beta-barrel. Binding of ferrichrome at the cell surface induces a conformational change in FhuA, but does not open the channel. Structural changes are propagated and amplified across the plug, and may facilitate binding of FhuA to TonB (PubMed:9865695, PubMed:9353297). TonB binding promotes conformational changes in outer surface-exposed loops of FhuA (PubMed:18653801). Phage T5 is a TonB-independent ligand, and its binding to FhuA results in the formation of high conductance ion channels (PubMed:8617231, PubMed:9353297).4 Publications
Sequence similaritiesi
Belongs to the TonB-dependent receptor family.Curated
Keywords - Domaini
Signal, TonB box, Transmembrane, Transmembrane beta strandPhylogenomic databases
eggNOGi | COG4774, Bacteria |
HOGENOMi | CLU_008287_9_0_6 |
InParanoidi | P06971 |
PhylomeDBi | P06971 |
Family and domain databases
Gene3Di | 2.170.130.10, 1 hit 2.40.170.20, 1 hit |
InterProi | View protein in InterPro IPR012910, Plug_dom IPR037066, Plug_dom_sf IPR039423, TonB-dep_rcpt IPR000531, TonB-dep_rcpt_b-brl IPR010916, TonB_box_CS IPR036942, TonB_rcpt_b-brl_sf IPR010917, TonB_rcpt_CS IPR010105, TonB_sidphr_rcpt |
PANTHERi | PTHR32552, PTHR32552, 1 hit |
Pfami | View protein in Pfam PF07715, Plug, 1 hit PF00593, TonB_dep_Rec, 1 hit |
TIGRFAMsi | TIGR01783, TonB-siderophor, 1 hit |
PROSITEi | View protein in PROSITE PS00430, TONB_DEPENDENT_REC_1, 1 hit PS01156, TONB_DEPENDENT_REC_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P06971-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARSKTAQPK HSLRKIAVVV ATAVSGMSVY AQAAVEPKED TITVTAAPAP
60 70 80 90 100
QESAWGPAAT IAARQSATGT KTDTPIQKVP QSISVVTAEE MALHQPKSVK
110 120 130 140 150
EALSYTPGVS VGTRGASNTY DHLIIRGFAA EGQSQNNYLN GLKLQGNFYN
160 170 180 190 200
DAVIDPYMLE RAEIMRGPVS VLYGKSSPGG LLNMVSKRPT TEPLKEVQFK
210 220 230 240 250
AGTDSLFQTG FDFSDSLDDD GVYSYRLTGL ARSANAQQKG SEEQRYAIAP
260 270 280 290 300
AFTWRPDDKT NFTFLSYFQN EPETGYYGWL PKEGTVEPLP NGKRLPTDFN
310 320 330 340 350
EGAKNNTYSR NEKMVGYSFD HEFNDTFTVR QNLRFAENKT SQNSVYGYGV
360 370 380 390 400
CSDPANAYSK QCAALAPADK GHYLARKYVV DDEKLQNFSV DTQLQSKFAT
410 420 430 440 450
GDIDHTLLTG VDFMRMRNDI NAWFGYDDSV PLLNLYNPVN TDFDFNAKDP
460 470 480 490 500
ANSGPYRILN KQKQTGVYVQ DQAQWDKVLV TLGGRYDWAD QESLNRVAGT
510 520 530 540 550
TDKRDDKQFT WRGGVNYLFD NGVTPYFSYS ESFEPSSQVG KDGNIFAPSK
560 570 580 590 600
GKQYEVGVKY VPEDRPIVVT GAVYNLTKTN NLMADPEGSF FSVEGGEIRA
610 620 630 640 650
RGVEIEAKAA LSASVNVVGS YTYTDAEYTT DTTYKGNTPA QVPKHMASLW
660 670 680 690 700
ADYTFFDGPL SGLTLGTGGR YTGSSYGDPA NSFKVGSYTV VDALVRYDLA
710 720 730 740
RVGMAGSNVA LHVNNLFDRE YVASCFNTYG CFWGAERQVV ATATFRF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 737 | R → P in CAA29253 (PubMed:2823072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12486 Genomic DNA Translation: AAB61768.1 U00096 Genomic DNA Translation: AAC73261.1 AP009048 Genomic DNA Translation: BAB96726.2 U70214 Genomic DNA Translation: AAB08580.1 X05810 Genomic DNA Translation: CAA29253.1 |
PIRi | F64738, QRECFE |
RefSeqi | NP_414692.1, NC_000913.3 WP_000124438.1, NZ_SSZK01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC73261; AAC73261; b0150 BAB96726; BAB96726; BAB96726 |
GeneIDi | 944856 |
KEGGi | ecj:JW0146 eco:b0150 |
PATRICi | fig|1411691.4.peg.2130 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12486 Genomic DNA Translation: AAB61768.1 U00096 Genomic DNA Translation: AAC73261.1 AP009048 Genomic DNA Translation: BAB96726.2 U70214 Genomic DNA Translation: AAB08580.1 X05810 Genomic DNA Translation: CAA29253.1 |
PIRi | F64738, QRECFE |
RefSeqi | NP_414692.1, NC_000913.3 WP_000124438.1, NZ_SSZK01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BY3 | X-ray | 2.74 | A | 34-747 | [»] | |
1BY5 | X-ray | 2.60 | A | 34-747 | [»] | |
1FCP | X-ray | 2.70 | A | 52-747 | [»] | |
1FI1 | X-ray | 2.90 | A | 52-747 | [»] | |
1QFF | X-ray | 2.70 | A | 34-747 | [»] | |
1QFG | X-ray | 2.50 | A | 34-747 | [»] | |
1QJQ | X-ray | 2.95 | A | 34-747 | [»] | |
1QKC | X-ray | 3.10 | A | 34-747 | [»] | |
2FCP | X-ray | 2.50 | A | 34-747 | [»] | |
2GRX | X-ray | 3.30 | A/B | 34-747 | [»] | |
4CU4 | X-ray | 2.30 | A | 53-747 | [»] | |
PCDDBi | P06971 | |||||
SMRi | P06971 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259745, 139 interactors |
ComplexPortali | CPX-2843, Ferrichrome outer membrane transporter complex |
DIPi | DIP-9602N |
IntActi | P06971, 7 interactors |
STRINGi | 511145.b0150 |
Chemistry databases
DrugBanki | DB02767, (R)-3-hydroxytetradecanoic acid DB02907, 2-Amino-Vinyl-Phosphate DB01814, 2-Tridecanoyloxy-Pentadecanoic Acid DB03548, 3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid DB02700, 3-Deoxy-D-Glucosamine DB04039, 3-Oxo-Pentadecanoic Acid DB02379, Beta-D-Glucose DB02613, Capric dimethyl amine oxide DB04220, CGP 4832 DB02724, Delta-2-Albomycin A1 DB03574, Ferricrocin-iron DB03111, Glucosamine 1-Phosphate DB02865, Glucosamine 4-Phosphate DB04526, L-Glycero-D-Manno-Heptopyranose DB03017, Lauric acid DB08231, Myristic acid DB02415, N-Octyl-2-Hydroxyethyl Sulfoxide DB02626, Phenylferricrocin-iron |
Protein family/group databases
TCDBi | 1.B.14.1.2, the outer membrane receptor (omr) family |
PTM databases
CarbonylDBi | P06971 |
Proteomic databases
PaxDbi | P06971 |
PRIDEi | P06971 |
Genome annotation databases
EnsemblBacteriai | AAC73261; AAC73261; b0150 BAB96726; BAB96726; BAB96726 |
GeneIDi | 944856 |
KEGGi | ecj:JW0146 eco:b0150 |
PATRICi | fig|1411691.4.peg.2130 |
Organism-specific databases
EchoBASEi | EB0298 |
Phylogenomic databases
eggNOGi | COG4774, Bacteria |
HOGENOMi | CLU_008287_9_0_6 |
InParanoidi | P06971 |
PhylomeDBi | P06971 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10302-MONOMER MetaCyc:EG10302-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P06971 |
PROi | PR:P06971 |
Family and domain databases
Gene3Di | 2.170.130.10, 1 hit 2.40.170.20, 1 hit |
InterProi | View protein in InterPro IPR012910, Plug_dom IPR037066, Plug_dom_sf IPR039423, TonB-dep_rcpt IPR000531, TonB-dep_rcpt_b-brl IPR010916, TonB_box_CS IPR036942, TonB_rcpt_b-brl_sf IPR010917, TonB_rcpt_CS IPR010105, TonB_sidphr_rcpt |
PANTHERi | PTHR32552, PTHR32552, 1 hit |
Pfami | View protein in Pfam PF07715, Plug, 1 hit PF00593, TonB_dep_Rec, 1 hit |
TIGRFAMsi | TIGR01783, TonB-siderophor, 1 hit |
PROSITEi | View protein in PROSITE PS00430, TONB_DEPENDENT_REC_1, 1 hit PS01156, TONB_DEPENDENT_REC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FHUA_ECOLI | |
Accessioni | P06971Primary (citable) accession number: P06971 Secondary accession number(s): P71280, P75665 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 207 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families