UniProtKB - P06951 (TERM_BPPZA)
DNA terminal protein
3
Functioni
Acts as a primer for DNA elongation during viral genomic replication. Acts as the small terminase protein during packaging. Recruits the phage DNA polymerase to the bacterial nucleoid. Primer terminal protein (TP) is covalently linked to the 5'-ends of both strands of the genome through a phosphodiester bond between the beta-hydroxyl group of a serine residue and the 5'-phosphate of the terminal deoxyadenylate (dAMP). To start replication, the DNA polymerase forms a heterodimer with a free TP that recognizes the replication origins at both 5' ends of the linear chromosome, and initiates replication using as primer the OH-group of Ser-232 of the TP. Since the polymerase initiates the replication on the second thymine, the TP-dAMP initiation product slides backwards to recover the template information of the first nucleotide.
By similarityHydrolyzes host peptidoglycans during virus entry.
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 230 | Positions the 3' end of the template strand at the active site of the DNA polymeraseBy similarity | 1 |
GO - Molecular functioni
- hydrolase activity Source: UniProtKB-KW
GO - Biological processi
- disruption by virus of host cell wall peptidoglycan during virus entry Source: UniProtKB-KW
- DNA replication, synthesis of RNA primer Source: InterPro
- protein-DNA covalent cross-linking Source: InterPro
- viral DNA genome replication Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: DNA terminal proteinAlternative name(s): Gene product 3 Short name: gp3 Protein p3 |
Gene namesi | Name:3 |
Organismi | Bacillus phage PZA (Bacteriophage PZA) |
Taxonomic identifieri | 10757 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Uroviricota › Caudoviricetes › Caudovirales › Salasmaviridae › Picovirinae › Salasvirus › Bacillus virus PZA |
Virus hosti | Bacillus subtilis [TaxID: 1423] |
Proteomesi |
|
Subcellular locationi
- Virion By similarity Note: Associates with the host bacterial nucleoid through its N-terminal region.By similarity
Keywords - Cellular componenti
VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000106557 | 1 – 266 | DNA terminal proteinAdd BLAST | 266 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 232 | O-(5'-phospho-DNA)-serineBy similarity | 1 |
Keywords - PTMi
Covalent protein-DNA linkage, PhosphoproteinInteractioni
Subunit structurei
Interacts with the viral polymerase; this interaction allows the initiation of TP-primed DNA replication at both viral DNA ends. Binds to ssDNA.
Interacts with the replication protein p1.
Part of a DNA-gp3-gp16 complex.
By similarityFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 73 | DisorderedBy similarityAdd BLAST | 73 | |
Regioni | 74 – 172 | Intermediate; makes extensive contacts with the phage DNA polymeraseBy similarityAdd BLAST | 99 | |
Regioni | 173 – 266 | PrimingBy similarityAdd BLAST | 94 | |
Regioni | 256 – 258 | Interaction with the viral DNA polymeraseBy similarity | 3 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 1.20.1270.230, 1 hit |
InterProi | View protein in InterPro IPR008770, DNA_terminal_Gp3 IPR043124, DNA_terminal_Gp3_C IPR037216, DNA_terminal_Gp3_sf |
Pfami | View protein in Pfam PF05435, Phi-29_GP3, 1 hit |
PIRSFi | PIRSF004179, Phi-29_GP3, 1 hit |
SUPFAMi | SSF140919, SSF140919, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MARSPRIRIK DNDKAEYARL VKNTKAKIAR TKKKYGVDLS AEINIPDLES
60 70 80 90 100
FETRAQFNKW KEQASSFTNR ANMRYQFEKN AYGVVASKAK IAEIERNTKE
110 120 130 140 150
VQRLVDEKIN AMKDKEYYAG GKPQGTIEQR IAMTSPAHVT GINRPRDFDF
160 170 180 190 200
SKVRTYSRLR TLEESMEMRT DPQYYEKKMI QLQLNFIKSV EGSFNSFDAA
210 220 230 240 250
DELIEELKKI PPDDFYELFL RISEISFEEF DSEGNTVENV EGNVYKILSY
260
LEQYRRGDFD LSLKGF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11813 Genomic DNA Translation: AAA88477.1 |
PIRi | E24528, ERBP3Z |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11813 Genomic DNA Translation: AAA88477.1 |
PIRi | E24528, ERBP3Z |
3D structure databases
SMRi | P06951 |
ModBasei | Search... |
Family and domain databases
Gene3Di | 1.20.1270.230, 1 hit |
InterProi | View protein in InterPro IPR008770, DNA_terminal_Gp3 IPR043124, DNA_terminal_Gp3_C IPR037216, DNA_terminal_Gp3_sf |
Pfami | View protein in Pfam PF05435, Phi-29_GP3, 1 hit |
PIRSFi | PIRSF004179, Phi-29_GP3, 1 hit |
SUPFAMi | SSF140919, SSF140919, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TERM_BPPZA | |
Accessioni | P06951Primary (citable) accession number: P06951 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 74 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |