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Protein

Protein E4

Gene

E4

Organism
Human papillomavirus type 5
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Contributes to multiple aspects of the viral life cycle including viral genome amplification, suppression of suprabasal cell differentiation and egress of newly formed virions. Induces host cell cycle arrest at the G2 phase by associating with and preventing the nuclear entry of host CDK1/cyclin B1 complexes. Inhibits cellular DNA replication by preventing loading of host replication licensing proteins MCM2 and MCM7 onto chromatin. Within the cytoplasm, associates with host kinase SRPK1, a splicing factor regulator, and inhibits its activity. Therefore, E4 favors expression of late viral transcripts by inhibiting SRPK1-mediated phosphorylation of host serine-arginine (SR) proteins that have critical roles in mRNA metabolism. Late in the infectious cycle, E4 also acts to diminish the integrity of the keratinocyte by disrupting the keratin cytoskeleton and inducing apoptosis through alteration of mitochondrial function to facilitate egress of the newly formed virions.By similarity

Miscellaneous

The major E4 form is first synthesized as an E1^E4 fusion protein from spliced E1^E4 transcripts, such that the first few amino acids of the E4 protein are derived from the N terminus of E1.By similarity

GO - Biological processi

Keywordsi

Biological processHost G2/M cell cycle arrest by virus, Host-virus interaction, Modulation of host cell cycle by virus

Names & Taxonomyi

Protein namesi
Recommended name:
Protein E4
Gene namesi
Name:E4
OrganismiHuman papillomavirus type 5
Taxonomic identifieri333923 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeFirstpapillomavirinaeBetapapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009252 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001332571 – 240Protein E4Add BLAST240

Post-translational modificationi

Phosphorylated by host ERK. The phosphorylation triggers a structural change that enhances keratin binding and protein stability.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP06924

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Assembles into oligomeric complexes. Interacts with host CDK1. Interacts with host SRPK1; this interaction may favor expression of late viral transcripts. Interacts with host cytokeratin components KRT8 and KRT18.By similarity

Structurei

3D structure databases

SMRiP06924
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the papillomaviridae E4 protein family.Curated

Phylogenomic databases

OrthoDBiVOG090001PD

Sequencei

Sequence statusi: Complete.

P06924-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTDPNSKAPR LQGRQEDKQT QTPPPRPPPP PQPPLTPRPD SSPHQNSHNK
60 70 80 90 100
PKPEEEGTDG GPPASQGDRK RSKGDQGPDT GPGLGPGRGP SPKPTPLGPP
110 120 130 140 150
PGPGPRRSPR LGPLQADRDP EEGPQPPAEG EVEGHPGGDQ GHPPPPPPAP
160 170 180 190 200
HNGHSGHEPK VQQPEGPEGR EGHEEGAVGG EGGDEEGHPP PPPPPTNGHE
210 220 230 240
GGLLSSVASL LVKWEGHFDQ LVQSIQDDLE DYWKKLATPQ
Length:240
Mass (Da):25,150
Last modified:January 18, 2017 - v3
Checksum:i6BE5CC0FAE47F107
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17463 Genomic DNA Translation: AAA46987.1 Sequence problems.
PIRiE26277 W4WL5
RefSeqiNP_041369.1, NC_001531.1

Genome annotation databases

GeneIDi1489051
KEGGivg:1489051

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17463 Genomic DNA Translation: AAA46987.1 Sequence problems.
PIRiE26277 W4WL5
RefSeqiNP_041369.1, NC_001531.1

3D structure databases

SMRiP06924
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP06924

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1489051
KEGGivg:1489051

Phylogenomic databases

OrthoDBiVOG090001PD

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiVE4_HPV05
AccessioniPrimary (citable) accession number: P06924
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 18, 2017
Last modified: June 20, 2018
This is version 45 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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