Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Urokinase-type plasminogen activator

Gene

Plau

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityi

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Activity regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei226Charge relay system1
Active sitei277Charge relay system1
Active sitei378Charge relay system1

GO - Molecular functioni

  • peptidase activity Source: MGI
  • serine-type endopeptidase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processPlasminogen activation

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-75205 Dissolution of Fibrin Clot

Protein family/group databases

MEROPSiS01.231

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase-type plasminogen activator (EC:3.4.21.73)
Short name:
U-plasminogen activator
Short name:
uPA
Cleaved into the following 3 chains:
Gene namesi
Name:Plau
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:97611 Plau

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075311

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000002832221 – 433Urokinase-type plasminogen activatorAdd BLAST413
ChainiPRO_000002832321 – 178Urokinase-type plasminogen activator long chain ABy similarityAdd BLAST158
ChainiPRO_0000028324157 – 178Urokinase-type plasminogen activator short chain ABy similarityAdd BLAST22
ChainiPRO_0000028325180 – 433Urokinase-type plasminogen activator chain BBy similarityAdd BLAST254

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi32 ↔ 401 Publication
Disulfide bondi34 ↔ 521 Publication
Disulfide bondi54 ↔ 631 Publication
Disulfide bondi71 ↔ 1521 Publication
Disulfide bondi92 ↔ 1341 Publication
Disulfide bondi123 ↔ 1471 Publication
Modified residuei159PhosphoserineBy similarity1
Disulfide bondi169 ↔ 301Interchain (between A and B chains)PROSITE-ProRule annotation
Disulfide bondi211 ↔ 227By similarity
Disulfide bondi219 ↔ 290By similarity
Disulfide bondi315 ↔ 384By similarity
Disulfide bondi347 ↔ 363By similarity
Disulfide bondi374 ↔ 402By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP06869
PeptideAtlasiP06869
PRIDEiP06869

PTM databases

PhosphoSitePlusiP06869

Expressioni

Gene expression databases

BgeeiENSMUSG00000021822 Expressed in 106 organ(s), highest expression level in entire extraembryonic component
CleanExiMM_PLAU
ExpressionAtlasiP06869 baseline and differential
GenevisibleiP06869 MM

Interactioni

Subunit structurei

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Slurp1Q9Z0K74EBI-8365661,EBI-14060702

Protein-protein interaction databases

BioGridi202230, 1 interactor
ComplexPortaliCPX-495 uPA-PAI-1 complex
CPX-510 uPA-uPAR complex
CPX-526 uPA-uPAR-vitronectin complex
CORUMiP06869
IntActiP06869, 4 interactors
MINTiP06869
STRINGi10090.ENSMUSP00000022368

Chemistry databases

BindingDBiP06869

Structurei

Secondary structure

1433
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP06869
SMRiP06869
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06869

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 64EGF-likePROSITE-ProRule annotationAdd BLAST37
Domaini71 – 152KringlePROSITE-ProRule annotationAdd BLAST82
Domaini180 – 426Peptidase S1PROSITE-ProRule annotationAdd BLAST247

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 58Binds urokinase plasminogen activator surface receptorBy similarityAdd BLAST24
Regioni153 – 179Connecting peptideAdd BLAST27

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGiENOG410IGFI Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000119133
HOGENOMiHOG000237314
HOVERGENiHBG008633
InParanoidiP06869
KOiK01348
OMAiKFQGEHC
OrthoDBiEOG091G0AH5
PhylomeDBiP06869
TreeFamiTF329901

Family and domain databases

CDDicd00108 KR, 1 hit
cd00190 Tryp_SPc, 1 hit
Gene3Di2.40.20.10, 1 hit
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000001 Kringle
IPR013806 Kringle-like
IPR018056 Kringle_CS
IPR038178 Kringle_sf
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR033116 TRYPSIN_SER
IPR034814 Urokinase
PANTHERiPTHR24264:SF38 PTHR24264:SF38, 1 hit
PfamiView protein in Pfam
PF00051 Kringle, 1 hit
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00130 KR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57440 SSF57440, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit
PS00021 KRINGLE_1, 1 hit
PS50070 KRINGLE_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P06869-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVWLASLFL CALVVKNSEG GSVLGAPDES NCGCQNGGVC VSYKYFSRIR
60 70 80 90 100
RCSCPRKFQG EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV
110 120 130 140 150
LQKPYNAHRP DAISLGLGKH NYCRNPDNQK RPWCYVQIGL RQFVQECMVH
160 170 180 190 200
DCSLSKKPSS SVDQQGFQCG QKALRPRFKI VGGEFTEVEN QPWFAAIYQK
210 220 230 240 250
NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY LGQSKESSYN
260 270 280 290 300
PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI
310 320 330 340 350
CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP
360 370 380 390 400
HYYGSEINYK MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR
410 420 430
GCAEKNKPGV YTRVSHFLDW IQSHIGEEKG LAF
Length:433
Mass (Da):48,268
Last modified:January 1, 1988 - v1
Checksum:iA99C35F6250443F9
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A286YE40A0A286YE40_MOUSE
Urokinase-type plasminogen activato...
Plau
35Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02389 mRNA Translation: CAA26231.1
M17922 Genomic DNA Translation: AAA40539.1
CCDSiCCDS26858.1
PIRiA29420 UKMS
RefSeqiNP_032899.1, NM_008873.3
XP_017171408.1, XM_017315919.1
UniGeneiMm.4183

Genome annotation databases

EnsembliENSMUST00000022368; ENSMUSP00000022368; ENSMUSG00000021822
GeneIDi18792
KEGGimmu:18792
UCSCiuc007skx.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02389 mRNA Translation: CAA26231.1
M17922 Genomic DNA Translation: AAA40539.1
CCDSiCCDS26858.1
PIRiA29420 UKMS
RefSeqiNP_032899.1, NM_008873.3
XP_017171408.1, XM_017315919.1
UniGeneiMm.4183

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LAQX-ray3.20A/B21-154[»]
5LHNX-ray2.55A180-426[»]
5LHPX-ray2.63A180-426[»]
5LHQX-ray2.60A180-426[»]
5LHRX-ray2.30A180-426[»]
5LHSX-ray3.05A/B/C/D180-426[»]
ProteinModelPortaliP06869
SMRiP06869
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202230, 1 interactor
ComplexPortaliCPX-495 uPA-PAI-1 complex
CPX-510 uPA-uPAR complex
CPX-526 uPA-uPAR-vitronectin complex
CORUMiP06869
IntActiP06869, 4 interactors
MINTiP06869
STRINGi10090.ENSMUSP00000022368

Chemistry databases

BindingDBiP06869
ChEMBLiCHEMBL1075311

Protein family/group databases

MEROPSiS01.231

PTM databases

PhosphoSitePlusiP06869

Proteomic databases

PaxDbiP06869
PeptideAtlasiP06869
PRIDEiP06869

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022368; ENSMUSP00000022368; ENSMUSG00000021822
GeneIDi18792
KEGGimmu:18792
UCSCiuc007skx.2 mouse

Organism-specific databases

CTDi5328
MGIiMGI:97611 Plau

Phylogenomic databases

eggNOGiENOG410IGFI Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000119133
HOGENOMiHOG000237314
HOVERGENiHBG008633
InParanoidiP06869
KOiK01348
OMAiKFQGEHC
OrthoDBiEOG091G0AH5
PhylomeDBiP06869
TreeFamiTF329901

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-75205 Dissolution of Fibrin Clot

Miscellaneous databases

EvolutionaryTraceiP06869
PROiPR:P06869
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021822 Expressed in 106 organ(s), highest expression level in entire extraembryonic component
CleanExiMM_PLAU
ExpressionAtlasiP06869 baseline and differential
GenevisibleiP06869 MM

Family and domain databases

CDDicd00108 KR, 1 hit
cd00190 Tryp_SPc, 1 hit
Gene3Di2.40.20.10, 1 hit
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000001 Kringle
IPR013806 Kringle-like
IPR018056 Kringle_CS
IPR038178 Kringle_sf
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR033116 TRYPSIN_SER
IPR034814 Urokinase
PANTHERiPTHR24264:SF38 PTHR24264:SF38, 1 hit
PfamiView protein in Pfam
PF00051 Kringle, 1 hit
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00130 KR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57440 SSF57440, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit
PS00021 KRINGLE_1, 1 hit
PS50070 KRINGLE_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUROK_MOUSE
AccessioniPrimary (citable) accession number: P06869
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 7, 2018
This is version 181 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again