UniProtKB - P06868 (PLMN_BOVIN)
Plasminogen
PLG
Functioni
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells (By similarity).
By similarityMiscellaneous
Catalytic activityi
- Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa, higher selectivity than trypsin. Converts fibrin into soluble products. EC:3.4.21.7
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 624 | Charge relay system | 1 | |
Active sitei | 667 | Charge relay system | 1 | |
Active sitei | 762 | Charge relay system | 1 |
GO - Molecular functioni
- protein domain specific binding Source: AgBase
- serine-type endopeptidase activity Source: UniProtKB-EC
- signaling receptor binding Source: AgBase
GO - Biological processi
- blood coagulation Source: UniProtKB-KW
- fibrinolysis Source: UniProtKB-KW
- response to heat Source: AgBase
- tissue remodeling Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Blood coagulation, Fibrinolysis, Hemostasis, Tissue remodeling |
Enzyme and pathway databases
SABIO-RKi | P06868 |
Protein family/group databases
MEROPSi | S01.233 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PLG |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Note: Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface (By similarity).By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | 1 PublicationAdd BLAST | 26 | |
ChainiPRO_0000028039 | 27 – 812 | PlasminogenAdd BLAST | 786 | |
ChainiPRO_0000028040 | 27 – 583 | Plasmin heavy chain AAdd BLAST | 557 | |
PeptideiPRO_0000028041 | 27 – 104 | Activation peptideBy similarityAdd BLAST | 78 | |
ChainiPRO_0000028042 | 105 – 583 | Plasmin heavy chain A, short formBy similarityAdd BLAST | 479 | |
ChainiPRO_0000028043 | 584 – 812 | Plasmin light chain BAdd BLAST | 229 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 56 ↔ 80 | By similarity | ||
Disulfide bondi | 60 ↔ 68 | By similarity | ||
Disulfide bondi | 110 ↔ 188 | By similarity | ||
Disulfide bondi | 131 ↔ 171 | By similarity | ||
Disulfide bondi | 159 ↔ 183 | By similarity | ||
Disulfide bondi | 192 ↔ 269 | By similarity | ||
Disulfide bondi | 195 ↔ 323 | By similarity | ||
Disulfide bondi | 213 ↔ 252 | By similarity | ||
Disulfide bondi | 241 ↔ 264 | By similarity | ||
Disulfide bondi | 282 ↔ 359 | By similarity | ||
Disulfide bondi | 303 ↔ 342 | By similarity | ||
GlycosylationiCAR_000014 | 315 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Disulfide bondi | 331 ↔ 354 | By similarity | ||
GlycosylationiCAR_000015 | 365 | O-linked (GalNAc...) serine2 Publications | 1 | |
Disulfide bondi | 384 ↔ 461 | By similarity | ||
Disulfide bondi | 405 ↔ 444 | By similarity | ||
Disulfide bondi | 433 ↔ 456 | By similarity | ||
Disulfide bondi | 485 ↔ 564 | By similarity | ||
Disulfide bondi | 506 ↔ 547 | By similarity | ||
Disulfide bondi | 535 ↔ 559 | By similarity | ||
Disulfide bondi | 570 ↔ 687 | Interchain (between A and B chains)By similarity | ||
Disulfide bondi | 580 ↔ 588 | Interchain (between A and B chains)By similarity | ||
Modified residuei | 600 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 609 ↔ 625 | By similarity | ||
Disulfide bondi | 701 ↔ 768 | By similarity | ||
Disulfide bondi | 731 ↔ 747 | By similarity | ||
Disulfide bondi | 758 ↔ 786 | By similarity |
Post-translational modificationi
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Phosphoprotein, ZymogenProteomic databases
PaxDbi | P06868 |
PeptideAtlasi | P06868 |
PRIDEi | P06868 |
PTM databases
GlyConnecti | 500, 12 N-Linked glycans (1 site), 2 O-Linked glycans (1 site) |
Interactioni
Subunit structurei
Interacts with CSPG4 and AMOT.
Interacts (via the Kringle domains) with HRG; the interaction tethers PLG to the cell surface and enhances its activation.
Interacts (via Kringle 4 domain) with ADA; the interaction stimulates PLG activation when in complex with DPP4. Angiostatin:
Interacts with ATP5F1A; the interaction inhibits most of the angiogenic effects of angiostatin.
By similarityGO - Molecular functioni
- protein domain specific binding Source: AgBase
- signaling receptor binding Source: AgBase
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000001674 |
Chemistry databases
BindingDBi | P06868 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 105 | PANPROSITE-ProRule annotationAdd BLAST | 77 | |
Domaini | 110 – 188 | Kringle 1PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 192 – 269 | Kringle 2PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 282 – 359 | Kringle 3PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 384 – 461 | Kringle 4PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 485 – 564 | Kringle 5PROSITE-ProRule annotationAdd BLAST | 80 | |
Domaini | 584 – 810 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 227 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Kringle, Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502QVNP, Eukaryota |
InParanoidi | P06868 |
OrthoDBi | 164039at2759 |
Family and domain databases
CDDi | cd00108, KR, 5 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 1 hit 2.40.20.10, 4 hits |
InterProi | View protein in InterPro IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR003609, Pan_app IPR023317, Pept_S1A_plasmin IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00051, Kringle, 5 hits PF00024, PAN_1, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001150, Plasmin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00130, KR, 5 hits SM00473, PAN_AP, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57440, SSF57440, 5 hits |
PROSITEi | View protein in PROSITE PS00021, KRINGLE_1, 5 hits PS50070, KRINGLE_2, 5 hits PS50948, PAN, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MLPASPKMEH KAVVFLLLLF LKSGLGDLLD DYVNTQGASL LSLSRKNLAG
60 70 80 90 100
RSVEDCAAKC EEETDFVCRA FQYHSKEQQC VVMAENSKNT PVFRMRDVIL
110 120 130 140 150
YEKRIYLLEC KTGNGQTYRG TTAETKSGVT CQKWSATSPH VPKFSPEKFP
160 170 180 190 200
LAGLEENYCR NPDNDENGPW CYTTDPDKRY DYCDIPECED KCMHCSGENY
210 220 230 240 250
EGKIAKTMSG RDCQAWDSQS PHAHGYIPSK FPNKNLKMNY CRNPDGEPRP
260 270 280 290 300
WCFTTDPQKR WEFCDIPRCT TPPPSSGPKY QCLKGTGKNY GGTVAVTESG
310 320 330 340 350
HTCQRWSEQT PHKHNRTPEN FPCKNLEENY CRNPNGEKAP WCYTTNSEVR
360 370 380 390 400
WEYCTIPSCE SSPLSTERMD VPVPPEQTPV PQDCYHGNGQ SYRGTSSTTI
410 420 430 440 450
TGRKCQSWSS MTPHRHLKTP ENYPNAGLTM NYCRNPDADK SPWCYTTDPR
460 470 480 490 500
VRWEFCNLKK CSETPEQVPA APQAPGVENP PEADCMIGTG KSYRGKKATT
510 520 530 540 550
VAGVPCQEWA AQEPHQHSIF TPETNPQSGL ERNYCRNPDG DVNGPWCYTM
560 570 580 590 600
NPRKPFDYCD VPQCESSFDC GKPKVEPKKC SGRIVGGCVS KPHSWPWQVS
610 620 630 640 650
LRRSSRHFCG GTLISPKWVL TAAHCLDNIL ALSFYKVILG AHNEKVREQS
660 670 680 690 700
VQEIPVSRLF REPSQADIAL LKLSRPAIIT KEVIPACLPP PNYMVAARTE
710 720 730 740 750
CYITGWGETQ GTFGEGLLKE AHLPVIENKV CNRNEYLDGR VKPTELCAGH
760 770 780 790 800
LIGGTDSCQG DSGGPLVCFE KDKYILQGVT SWGLGCARPN KPGVYVRVSP
810
YVPWIEETMR RN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 335 | N → D AA sequence (PubMed:3846532).Curated | 1 | |
Sequence conflicti | 516 | Q → H AA sequence (PubMed:3846532).Curated | 1 | |
Sequence conflicti | 555 | P → L AA sequence (PubMed:3846532).Curated | 1 | |
Sequence conflicti | 744 | T → R in AAA30714 (PubMed:6148961).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X79402 mRNA Translation: CAA55939.1 K02935 mRNA Translation: AAA30714.1 |
PIRi | S45046, PLBO |
RefSeqi | NP_776376.1, NM_173951.2 |
Genome annotation databases
GeneIDi | 280897 |
KEGGi | bta:280897 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X79402 mRNA Translation: CAA55939.1 K02935 mRNA Translation: AAA30714.1 |
PIRi | S45046, PLBO |
RefSeqi | NP_776376.1, NM_173951.2 |
3D structure databases
AlphaFoldDBi | P06868 |
SMRi | P06868 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000001674 |
Chemistry databases
BindingDBi | P06868 |
ChEMBLi | CHEMBL2957 |
Protein family/group databases
MEROPSi | S01.233 |
PTM databases
GlyConnecti | 500, 12 N-Linked glycans (1 site), 2 O-Linked glycans (1 site) |
Proteomic databases
PaxDbi | P06868 |
PeptideAtlasi | P06868 |
PRIDEi | P06868 |
Genome annotation databases
GeneIDi | 280897 |
KEGGi | bta:280897 |
Organism-specific databases
CTDi | 5340 |
Phylogenomic databases
eggNOGi | ENOG502QVNP, Eukaryota |
InParanoidi | P06868 |
OrthoDBi | 164039at2759 |
Enzyme and pathway databases
SABIO-RKi | P06868 |
Miscellaneous databases
PROi | PR:P06868 |
Family and domain databases
CDDi | cd00108, KR, 5 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 1 hit 2.40.20.10, 4 hits |
InterProi | View protein in InterPro IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR003609, Pan_app IPR023317, Pept_S1A_plasmin IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00051, Kringle, 5 hits PF00024, PAN_1, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001150, Plasmin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00130, KR, 5 hits SM00473, PAN_AP, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57440, SSF57440, 5 hits |
PROSITEi | View protein in PROSITE PS00021, KRINGLE_1, 5 hits PS50070, KRINGLE_2, 5 hits PS50948, PAN, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PLMN_BOVIN | |
Accessioni | P06868Primary (citable) accession number: P06868 Secondary accession number(s): Q28162 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 169 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families