UniProtKB - P06864 (BGA2_ECOLI)
Protein
Evolved beta-galactosidase subunit alpha
Gene
ebgA
Organism
Escherichia coli (strain K12)
Status
Functioni
The wild-type enzyme is an ineffective lactase. Two classes of point mutations dramatically improve activity of the enzyme.
Catalytic activityi
- Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. EC:3.2.1.23
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 449 | Proton donor1 Publication | 1 | |
Active sitei | 512 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- beta-galactosidase activity Source: GO_Central
- carbohydrate binding Source: InterPro
GO - Biological processi
- lactose catabolic process Source: GO_Central
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10252-MONOMER |
SABIO-RKi | P06864 |
Protein family/group databases
CAZyi | GH2, Glycoside Hydrolase Family 2 |
Names & Taxonomyi
Protein namesi | Recommended name: Evolved beta-galactosidase subunit alpha (EC:3.2.1.23)Short name: Beta-gal Alternative name(s): Lactase |
Gene namesi | Name:ebgA Ordered Locus Names:b3076, JW5511 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057651 | 1 – 1030 | Evolved beta-galactosidase subunit alphaAdd BLAST | 1030 |
Proteomic databases
PaxDbi | P06864 |
PRIDEi | P06864 |
Interactioni
Subunit structurei
Heterooctamer of 4 alpha and 4 beta subunits.
1 PublicationBinary interactionsi
P06864
With | #Exp. | IntAct |
---|---|---|
intA [P32053] | 3 | EBI-558098,EBI-552967 |
Protein-protein interaction databases
BioGRIDi | 4263419, 6 interactors 851899, 6 interactors |
DIPi | DIP-2893N |
IntActi | P06864, 7 interactors |
STRINGi | 511145.b3076 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 2 family.Curated
Phylogenomic databases
eggNOGi | COG3250, Bacteria |
HOGENOMi | CLU_002346_0_2_6 |
InParanoidi | P06864 |
PhylomeDBi | P06864 |
Family and domain databases
Gene3Di | 2.60.120.260, 1 hit 2.60.40.10, 2 hits 2.70.98.10, 1 hit |
InterProi | View protein in InterPro IPR004199, B-gal_small/dom_5 IPR036156, Beta-gal/glucu_dom_sf IPR011013, Gal_mutarotase_sf_dom IPR008979, Galactose-bd-like_sf IPR014718, GH-type_carb-bd IPR006101, Glyco_hydro_2 IPR023232, Glyco_hydro_2_AS IPR023933, Glyco_hydro_2_beta_Galsidase IPR006103, Glyco_hydro_2_cat IPR023230, Glyco_hydro_2_CS IPR006102, Glyco_hydro_2_Ig-like IPR006104, Glyco_hydro_2_N IPR017853, Glycoside_hydrolase_SF IPR013783, Ig-like_fold IPR032312, LacZ_4 |
PANTHERi | PTHR46323, PTHR46323, 1 hit |
Pfami | View protein in Pfam PF02929, Bgal_small_N, 1 hit PF16353, DUF4981, 1 hit PF00703, Glyco_hydro_2, 1 hit PF02836, Glyco_hydro_2_C, 1 hit PF02837, Glyco_hydro_2_N, 1 hit |
PRINTSi | PR00132, GLHYDRLASE2 |
SMARTi | View protein in SMART SM01038, Bgal_small_N, 1 hit |
SUPFAMi | SSF49303, SSF49303, 2 hits SSF49785, SSF49785, 1 hit SSF51445, SSF51445, 1 hit SSF74650, SSF74650, 1 hit |
PROSITEi | View protein in PROSITE PS00719, GLYCOSYL_HYDROL_F2_1, 1 hit PS00608, GLYCOSYL_HYDROL_F2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P06864-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNRWENIQLT HENRLAPRAY FFSYDSVAQA RTFARETSSL FLPLSGQWNF
60 70 80 90 100
HFFDHPLQVP EAFTSELMAD WGHITVPAMW QMEGHGKLQY TDEGFPFPID
110 120 130 140 150
VPFVPSDNPT GAYQRIFTLS DGWQGKQTLI KFDGVETYFE VYVNGQYVGF
160 170 180 190 200
SKGSRLTAEF DISAMVKTGD NLLCVRVMQW ADSTYVEDQD MWWSAGIFRD
210 220 230 240 250
VYLVGKHLTH INDFTVRTDF DEAYCDATLS CEVVLENLAA SPVVTTLEYT
260 270 280 290 300
LFDGERVVHS SAIDHLAIEK LTSASFAFTV EQPQQWSAES PYLYHLVMTL
310 320 330 340 350
KDANGNVLEV VPQRVGFRDI KVRDGLFWIN NRYVMLHGVN RHDNDHRKGR
360 370 380 390 400
AVGMDRVEKD LQLMKQHNIN SVRTAHYPND PRFYELCDIY GLFVMAETDV
410 420 430 440 450
ESHGFANVGD ISRITDDPQW EKVYVERIVR HIHAQKNHPS IIIWSLGNES
460 470 480 490 500
GYGCNIRAMY HAAKALDDTR LVHYEEDRDA EVVDIISTMY TRVPLMNEFG
510 520 530 540 550
EYPHPKPRII CEYAHAMGNG PGGLTEYQNV FYKHDCIQGH YVWEWCDHGI
560 570 580 590 600
QAQDDHGNVW YKFGGDYGDY PNNYNFCLDG LIYSDQTPGP GLKEYKQVIA
610 620 630 640 650
PVKIHARDLT RGELKVENKL WFTTLDDYTL HAEVRAEGET LATQQIKLRD
660 670 680 690 700
VAPNSEAPLQ ITLPQLDARE AFLNITVTKD SRTRYSEAGH PIATYQFPLK
710 720 730 740 750
ENTAQPVPFA PNNARPLTLE DDRLSCTVRG YNFAITFSKM SGKPTSWQVN
760 770 780 790 800
GESLLTREPK INFFKPMIDN HKQEYEGLWQ PNHLQIMQEH LRDFAVEQSD
810 820 830 840 850
GEVLIISRTV IAPPVFDFGM RCTYIWRIAA DGQVNVALSG ERYGDYPHII
860 870 880 890 900
PCIGFTMGIN GEYDQVAYYG RGPGENYADS QQANIIDIWR STVDAMFENY
910 920 930 940 950
PFPQNNGNRQ HVRWTALTNR HGNGLLVVPQ RPINFSAWHY TQENIHAAQH
960 970 980 990 1000
CNELQRSDDI TLNLDHQLLG LGSNSWGSEV LDSWRVWFRD FSYGFTLLPV
1010 1020 1030
SGGEATAQSL ASYEFGAGFF STNLHSENKQ
Sequence cautioni
The sequence AAA57877 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 275 | S → T (PubMed:2515108).Curated | 1 | |
Sequence conflicti | 275 | S → T (PubMed:3939707).Curated | 1 | |
Sequence conflicti | 275 | S → T in AAA57877 (PubMed:9278503).Curated | 1 | |
Sequence conflicti | 465 | A → R (PubMed:2515108).Curated | 1 | |
Sequence conflicti | 465 | A → R (PubMed:3939707).Curated | 1 | |
Sequence conflicti | 640 | T → S (PubMed:2515108).Curated | 1 | |
Sequence conflicti | 640 | T → S (PubMed:3939707).Curated | 1 | |
Sequence conflicti | 649 | R → P (PubMed:2515108).Curated | 1 | |
Sequence conflicti | 649 | R → P (PubMed:3939707).Curated | 1 | |
Sequence conflicti | 767 | M → MM (PubMed:2515108).Curated | 1 | |
Sequence conflicti | 767 | M → MM (PubMed:3939707).Curated | 1 | |
Sequence conflicti | 891 – 892 | ST → QA (PubMed:2515108).Curated | 2 | |
Sequence conflicti | 891 – 892 | ST → QA (PubMed:3939707).Curated | 2 | |
Sequence conflicti | 891 – 892 | ST → QA in AAA57877 (PubMed:9278503).Curated | 2 | |
Sequence conflicti | 1026 | S → T (PubMed:2515108).Curated | 1 | |
Sequence conflicti | 1026 | S → T (PubMed:3939707).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 92 | D → N Improve activity. | 1 | |
Natural varianti | 93 | E → K Improve activity. | 1 | |
Natural varianti | 976 | W → C Improve activity. | 1 | |
Natural varianti | 978 | S → G Improve activity. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64441 Genomic DNA Translation: AAA61971.1 X52031 Genomic DNA Translation: CAA36274.1 X03228 Genomic DNA Translation: CAA26977.1 Sequence problems. U18997 Genomic DNA Translation: AAA57877.1 Different initiation. U00096 Genomic DNA Translation: AAT48164.1 AP009048 Genomic DNA Translation: BAE77126.1 |
PIRi | A65096, GBECE |
RefSeqi | WP_001082856.1, NZ_LN832404.1 YP_026199.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48164; AAT48164; b3076 BAE77126; BAE77126; BAE77126 |
GeneIDi | 947583 |
KEGGi | ecj:JW5511 eco:b3076 |
PATRICi | fig|511145.12.peg.3170 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64441 Genomic DNA Translation: AAA61971.1 X52031 Genomic DNA Translation: CAA36274.1 X03228 Genomic DNA Translation: CAA26977.1 Sequence problems. U18997 Genomic DNA Translation: AAA57877.1 Different initiation. U00096 Genomic DNA Translation: AAT48164.1 AP009048 Genomic DNA Translation: BAE77126.1 |
PIRi | A65096, GBECE |
RefSeqi | WP_001082856.1, NZ_LN832404.1 YP_026199.1, NC_000913.3 |
3D structure databases
SMRi | P06864 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263419, 6 interactors 851899, 6 interactors |
DIPi | DIP-2893N |
IntActi | P06864, 7 interactors |
STRINGi | 511145.b3076 |
Chemistry databases
DrugBanki | DB00581, Lactulose |
Protein family/group databases
CAZyi | GH2, Glycoside Hydrolase Family 2 |
Proteomic databases
PaxDbi | P06864 |
PRIDEi | P06864 |
Genome annotation databases
EnsemblBacteriai | AAT48164; AAT48164; b3076 BAE77126; BAE77126; BAE77126 |
GeneIDi | 947583 |
KEGGi | ecj:JW5511 eco:b3076 |
PATRICi | fig|511145.12.peg.3170 |
Organism-specific databases
EchoBASEi | EB0248 |
Phylogenomic databases
eggNOGi | COG3250, Bacteria |
HOGENOMi | CLU_002346_0_2_6 |
InParanoidi | P06864 |
PhylomeDBi | P06864 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10252-MONOMER |
SABIO-RKi | P06864 |
Miscellaneous databases
PROi | PR:P06864 |
Family and domain databases
Gene3Di | 2.60.120.260, 1 hit 2.60.40.10, 2 hits 2.70.98.10, 1 hit |
InterProi | View protein in InterPro IPR004199, B-gal_small/dom_5 IPR036156, Beta-gal/glucu_dom_sf IPR011013, Gal_mutarotase_sf_dom IPR008979, Galactose-bd-like_sf IPR014718, GH-type_carb-bd IPR006101, Glyco_hydro_2 IPR023232, Glyco_hydro_2_AS IPR023933, Glyco_hydro_2_beta_Galsidase IPR006103, Glyco_hydro_2_cat IPR023230, Glyco_hydro_2_CS IPR006102, Glyco_hydro_2_Ig-like IPR006104, Glyco_hydro_2_N IPR017853, Glycoside_hydrolase_SF IPR013783, Ig-like_fold IPR032312, LacZ_4 |
PANTHERi | PTHR46323, PTHR46323, 1 hit |
Pfami | View protein in Pfam PF02929, Bgal_small_N, 1 hit PF16353, DUF4981, 1 hit PF00703, Glyco_hydro_2, 1 hit PF02836, Glyco_hydro_2_C, 1 hit PF02837, Glyco_hydro_2_N, 1 hit |
PRINTSi | PR00132, GLHYDRLASE2 |
SMARTi | View protein in SMART SM01038, Bgal_small_N, 1 hit |
SUPFAMi | SSF49303, SSF49303, 2 hits SSF49785, SSF49785, 1 hit SSF51445, SSF51445, 1 hit SSF74650, SSF74650, 1 hit |
PROSITEi | View protein in PROSITE PS00719, GLYCOSYL_HYDROL_F2_1, 1 hit PS00608, GLYCOSYL_HYDROL_F2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BGA2_ECOLI | |
Accessioni | P06864Primary (citable) accession number: P06864 Secondary accession number(s): P76660, Q2M9D0, Q6BF50 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | October 11, 2004 | |
Last modified: | December 2, 2020 | |
This is version 178 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families