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Protein

Polynucleotide kinase

Gene

pseT

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a 5'-hydroxyl kinase, a 3'-phosphatase and a 2',3'-cyclic phosphodiesterase. Catalyzes the transfer of the terminal phosphate of ATP to the 5'-hydroxyl termini of ribo- and deoxyribonucleotides. In the presence of ADP the enzyme also catalyzes an exchange reaction. In the exchange reaction, an excess ADP causes the enzyme to transfer the 5' terminal phosphate from phosphorylated DNA to ADP (PubMed:5323016, PubMed:199248). Involved in countering a host defense mechanism which activates T4-induced anticodon nuclease and shuts off viral translation. The polynucleotide kinase modifies the ends of nicked tRNA generated by the antiviral response of the host bacteria and facilitates repair by T4 RNA ligase (PubMed:2444436).4 Publications

Catalytic activityi

ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.
A deoxyribonucleoside 3'-phosphate + H2O = a deoxyribonucleoside + phosphate.1 Publication

Cofactori

Mg2+2 Publications

pH dependencei

Optimum pH is 6.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Kinase, Transferase
Biological processDNA damage, DNA repair, Evasion of bacteria-mediated translation shutoff by virus, Host-virus interaction
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.78 732
SABIO-RKiP06855

Names & Taxonomyi

Protein namesi
Recommended name:
Polynucleotide kinase (EC:2.7.1.78)
Short name:
PNK
Alternative name(s):
Deoxynucleotide 3'-phosphatase1 Publication (EC:3.1.3.341 Publication)
Polynucleotide 5'-hydroxyl-kinase
Gene namesi
Name:pseT
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome
  • UP000001092 Componenti: Genome
  • UP000185270 Componenti: Genome
  • UP000185269 Componenti: Genome
  • UP000185271 Componenti: Genome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5292

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001649491 – 301Polynucleotide kinaseAdd BLAST301

Proteomic databases

PRIDEiP06855

Interactioni

Subunit structurei

Homotetramer.

Chemistry databases

BindingDBiP06855

Structurei

Secondary structure

1301
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP06855
SMRiP06855
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06855

Family & Domainsi

Phylogenomic databases

OrthoDBiVOG0900002N

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR027417 P-loop_NTPase
SUPFAMiSSF52540 SSF52540, 1 hit
SSF56784 SSF56784, 1 hit

Sequencei

Sequence statusi: Complete.

P06855-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKIILTIGC PGSGKSTWAR EFIAKNPGFY NINRDDYRQS IMAHEERDEY
60 70 80 90 100
KYTKKKEGIV TGMQFDTAKS ILYGGDSVKG VIISDTNLNP ERRLAWETFA
110 120 130 140 150
KEYGWKVEHK VFDVPWTELV KRNSKRGTKA VPIDVLRSMY KSMREYLGLP
160 170 180 190 200
VYNGTPGKPK AVIFDVDGTL AKMNGRGPYD LEKCDTDVIN PMVVELSKMY
210 220 230 240 250
ALMGYQIVVV SGRESGTKED PTKYYRMTRK WVEDIAGVPL VMQCQREQGD
260 270 280 290 300
TRKDDVVKEE IFWKHIAPHF DVKLAIDDRT QVVEMWRRIG VECWQVASGD

F
Length:301
Mass (Da):34,620
Last modified:January 1, 1988 - v1
Checksum:i0EAFB6BA83236D31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03007 Genomic DNA Translation: CAA26792.1
AF158101 Genomic DNA Translation: AAD42642.1
HM137666 Genomic DNA Translation: ADJ39944.1
KJ477684 Genomic DNA Translation: AHY83527.1
KJ477685 Genomic DNA Translation: AHY83726.1
KJ477686 Genomic DNA Translation: AHY83916.1
PIRiA24642 KIBPP4
RefSeqiNP_049834.1, NC_000866.4

Genome annotation databases

GeneIDi1258796
KEGGivg:1258796

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03007 Genomic DNA Translation: CAA26792.1
AF158101 Genomic DNA Translation: AAD42642.1
HM137666 Genomic DNA Translation: ADJ39944.1
KJ477684 Genomic DNA Translation: AHY83527.1
KJ477685 Genomic DNA Translation: AHY83726.1
KJ477686 Genomic DNA Translation: AHY83916.1
PIRiA24642 KIBPP4
RefSeqiNP_049834.1, NC_000866.4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LTQX-ray2.33A1-301[»]
1LY1X-ray2.00A1-181[»]
1RC8X-ray2.75A1-301[»]
1RPZX-ray2.90A1-301[»]
1RRCX-ray2.46A1-301[»]
2IA5X-ray2.90A/B/C/D/E/F/G/H/I/J/K/L1-301[»]
5UJ0X-ray2.30A/B158-301[»]
ProteinModelPortaliP06855
SMRiP06855
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP06855
ChEMBLiCHEMBL5292

Proteomic databases

PRIDEiP06855

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258796
KEGGivg:1258796

Phylogenomic databases

OrthoDBiVOG0900002N

Enzyme and pathway databases

BRENDAi2.7.1.78 732
SABIO-RKiP06855

Miscellaneous databases

EvolutionaryTraceiP06855
PROiPR:P06855

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR027417 P-loop_NTPase
SUPFAMiSSF52540 SSF52540, 1 hit
SSF56784 SSF56784, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKIPN_BPT4
AccessioniPrimary (citable) accession number: P06855
Secondary accession number(s): D9IEQ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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