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Protein

Holin

Gene

t

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space. Determines the precise timing of host cell lysis. Participates with the endolysin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles from the host cell.UniRule annotation2 Publications

GO - Molecular functioni

  • canonical holin activity Source: CACAO

GO - Biological processi

Keywordsi

Biological processCytolysis, Host cell lysis by virus, Viral release from host cell

Protein family/group databases

TCDBi1.E.8.1.1 the t4 holin (t4 holin) family

Names & Taxonomyi

Protein namesi
Recommended name:
HolinUniRule annotation
Alternative name(s):
Lysis protein
Protein rV
Gene namesi
Name:t
Synonyms:rV
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 34CytoplasmicUniRule annotation1 PublicationAdd BLAST34
Transmembranei35 – 49Helical; Signal-anchor for type II membrane proteinUniRule annotation1 PublicationAdd BLAST15
Topological domaini50 – 218PeriplasmicUniRule annotation1 PublicationAdd BLAST169

GO - Cellular componenti

  • host cell plasma membrane Source: CACAO
  • integral component of membrane Source: UniProtKB-KW

Keywords - Cellular componenti

Host cell inner membrane, Host cell membrane, Host membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi175C → S: Complete loss of lysis. 1 Publication1
Mutagenesisi207C → S: Complete loss of lysis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001650661 – 218HolinAdd BLAST218

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi175 ↔ 207UniRule annotation1 Publication

Post-translational modificationi

Disulfide bond is required for functionality.UniRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homomultimer. Interacts (via C-terminus) with antiholin (via C-terminus); this interaction blocks the holin homomultimerization and delays host cell lysis (PubMed:16166524). Interacts (via N-terminus) with the lysis inhibition accessory protein rIII; this interaction stabilizes the holin-antiholin complex thereby resulting in a robust block of the hole formation (PubMed:27381920).UniRule annotation2 Publications

Family & Domainsi

Sequence similaritiesi

Belongs to the T4likevirus holin family.UniRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090000P3

Family and domain databases

HAMAPiMF_04104 HOLIN_T4, 1 hit
InterProiView protein in InterPro
IPR020982 Phage_T4_GpT_holin
PfamiView protein in Pfam
PF11031 Phage_holin_T, 1 hit

Sequencei

Sequence statusi: Complete.

P06808-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAPRISFSP SDILFGVLDR LFKDNATGKV LASRVAVVIL LFIMAIVWYR
60 70 80 90 100
GDSFFEYYKQ SKYETYSEII EKERTARFES VALEQLQIVH ISSEADFSAV
110 120 130 140 150
YSFRPKNLNY FVDIIAYEGK LPSTISEKSL GGYPVDKTMD EYTVHLNGRH
160 170 180 190 200
YYSNSKFAFL PTKKPTPEIN YMYSCPYFNL DNIYAGTITM YWYRNDHISN
210
DRLESICAQA ARILGRAK
Length:218
Mass (Da):25,176
Last modified:January 1, 1988 - v1
Checksum:i9110BE111D772DF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00408 Genomic DNA Translation: CAA68470.1
AF158101 Genomic DNA Translation: AAD42661.1
X05677 Genomic DNA Translation: CAA29164.1
M99441 Genomic DNA Translation: AAA32481.1
PIRiJF0028 YVBPT4
RefSeqiNP_049865.1, NC_000866.4

Genome annotation databases

GeneIDi1258610
KEGGivg:1258610

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00408 Genomic DNA Translation: CAA68470.1
AF158101 Genomic DNA Translation: AAD42661.1
X05677 Genomic DNA Translation: CAA29164.1
M99441 Genomic DNA Translation: AAA32481.1
PIRiJF0028 YVBPT4
RefSeqiNP_049865.1, NC_000866.4

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.E.8.1.1 the t4 holin (t4 holin) family

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258610
KEGGivg:1258610

Phylogenomic databases

OrthoDBiVOG090000P3

Family and domain databases

HAMAPiMF_04104 HOLIN_T4, 1 hit
InterProiView protein in InterPro
IPR020982 Phage_T4_GpT_holin
PfamiView protein in Pfam
PF11031 Phage_holin_T, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHOLIN_BPT4
AccessioniPrimary (citable) accession number: P06808
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 25, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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