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Protein

NADP-dependent malic enzyme

Gene

Me1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102Proton donorBy similarity1
Binding sitei155NADPBy similarity1
Active sitei173Proton acceptorBy similarity1
Metal bindingi245Divalent metal cationBy similarity1
Metal bindingi246Divalent metal cationBy similarity1
Metal bindingi269Divalent metal cationBy similarity1
Binding sitei269NADPBy similarity1
Sitei269Important for activityBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi301 – 318NADPBy similarityAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NADP

Enzyme and pathway databases

ReactomeiR-MMU-70268 Pyruvate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent malic enzyme (EC:1.1.1.40)
Short name:
NADP-ME
Alternative name(s):
Malic enzyme 1
Gene namesi
Name:Me1
Synonyms:Mod-1, Mod1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:97043 Me1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001601931 – 572NADP-dependent malic enzymeAdd BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei336PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP06801
MaxQBiP06801
PaxDbiP06801
PeptideAtlasiP06801
PRIDEiP06801

2D gel databases

REPRODUCTION-2DPAGEiP06801
SWISS-2DPAGEiP06801

PTM databases

iPTMnetiP06801
PhosphoSitePlusiP06801

Expressioni

Gene expression databases

BgeeiENSMUSG00000032418 Expressed in 302 organ(s), highest expression level in adrenal gland
CleanExiMM_ME1
ExpressionAtlasiP06801 baseline and differential
GenevisibleiP06801 MM

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

IntActiP06801, 4 interactors
MINTiP06801
STRINGi10090.ENSMUSP00000034989

Structurei

3D structure databases

ProteinModelPortaliP06801
SMRiP06801
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Phylogenomic databases

eggNOGiKOG1257 Eukaryota
COG0281 LUCA
GeneTreeiENSGT00390000000754
HOGENOMiHOG000042486
HOVERGENiHBG000746
InParanoidiP06801
KOiK00029
OrthoDBiEOG091G04H9
TreeFamiTF300537

Family and domain databases

Gene3Di3.40.50.10380, 1 hit
InterProiView protein in InterPro
IPR015884 Malic_enzyme_CS
IPR012301 Malic_N_dom
IPR037062 Malic_N_dom_sf
IPR012302 Malic_NAD-bd
IPR001891 Malic_OxRdtase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00390 malic, 1 hit
PF03949 Malic_M, 1 hit
PIRSFiPIRSF000106 ME, 1 hit
PRINTSiPR00072 MALOXRDTASE
SMARTiView protein in SMART
SM01274 malic, 1 hit
SM00919 Malic_M, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00331 MALIC_ENZYMES, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P06801-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEPRAPRRRH THQRGYLLTR DPHLNKDLAF TLEERQQLNI HGLLPPCIIS
60 70 80 90 100
QELQVLRIIK NFERLNSDFD RYLLLMDLQD RNEKLFYSVL MSDVEKFMPI
110 120 130 140 150
VYTPTVGLAC QQYSLAFRKP RGLFISIHDK GHIASVLNAW PEDVVKAIVV
160 170 180 190 200
TDGERILGLG DLGCNGMGIP VGKLALYTAC GGVNPQQCLP ITLDVGTENE
210 220 230 240 250
ELLKDPLYIG LRHRRVRGPE YDAFLDEFME AASSKYGMNC LIQFEDFANR
260 270 280 290 300
NAFRLLNKYR NKYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTVLFQ
310 320 330 340 350
GAGEAALGIA HLVVMAMEKE GLSKENARKK IWLVDSKGLI VKGRASLTEE
360 370 380 390 400
KEVFAHEHEE MKNLEAIVQK IKPTALIGVA AIGGAFTEQI LKDMAAFNER
410 420 430 440 450
PIIFALSNPT SKAECSAEQC YKVTKGRAIF ASGSPFDPVT LPDGRTLFPG
460 470 480 490 500
QGNNSYVFPG VALGVVACGL RHIDDKVFLT TAEVISQQVS DKHLQEGRLY
510 520 530 540 550
PPLNTIRGVS LKIAVKIVQD AYKEKMATVY PEPQNKEEFV SSQMYSTNYD
560 570
QILPDCYPWP AEVQKIQTKV NQ
Length:572
Mass (Da):63,954
Last modified:July 27, 2011 - v2
Checksum:iD6BBF93FBEDAB1BE
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3TQP6Q3TQP6_MOUSE
Malic enzyme
Me1 Mod1
552Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti408N → S in AAA39727 (PubMed:3805042).Curated1
Sequence conflicti408N → S in AAA39489 (PubMed:3541755).Curated1
Sequence conflicti418 – 419EQ → DE in AAA39727 (PubMed:3805042).Curated2
Sequence conflicti418 – 419EQ → DE in AAA39489 (PubMed:3541755).Curated2
Sequence conflicti482A → R in AAA39727 (PubMed:3805042).Curated1
Sequence conflicti482A → R in AAA39489 (PubMed:3541755).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02652 mRNA Translation: AAA39727.1
M26756 mRNA Translation: AAA39489.1
AK004980 mRNA Translation: BAB23716.1
AK077968 mRNA Translation: BAC37086.1
CCDSiCCDS23383.1
PIRiA26683 DEMSMX
RefSeqiNP_001185862.1, NM_001198933.1
NP_032641.2, NM_008615.2
UniGeneiMm.148155

Genome annotation databases

EnsembliENSMUST00000034989; ENSMUSP00000034989; ENSMUSG00000032418
GeneIDi17436
KEGGimmu:17436
UCSCiuc009qxs.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02652 mRNA Translation: AAA39727.1
M26756 mRNA Translation: AAA39489.1
AK004980 mRNA Translation: BAB23716.1
AK077968 mRNA Translation: BAC37086.1
CCDSiCCDS23383.1
PIRiA26683 DEMSMX
RefSeqiNP_001185862.1, NM_001198933.1
NP_032641.2, NM_008615.2
UniGeneiMm.148155

3D structure databases

ProteinModelPortaliP06801
SMRiP06801
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP06801, 4 interactors
MINTiP06801
STRINGi10090.ENSMUSP00000034989

PTM databases

iPTMnetiP06801
PhosphoSitePlusiP06801

2D gel databases

REPRODUCTION-2DPAGEiP06801
SWISS-2DPAGEiP06801

Proteomic databases

EPDiP06801
MaxQBiP06801
PaxDbiP06801
PeptideAtlasiP06801
PRIDEiP06801

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034989; ENSMUSP00000034989; ENSMUSG00000032418
GeneIDi17436
KEGGimmu:17436
UCSCiuc009qxs.2 mouse

Organism-specific databases

CTDi4199
MGIiMGI:97043 Me1

Phylogenomic databases

eggNOGiKOG1257 Eukaryota
COG0281 LUCA
GeneTreeiENSGT00390000000754
HOGENOMiHOG000042486
HOVERGENiHBG000746
InParanoidiP06801
KOiK00029
OrthoDBiEOG091G04H9
TreeFamiTF300537

Enzyme and pathway databases

ReactomeiR-MMU-70268 Pyruvate metabolism

Miscellaneous databases

ChiTaRSiMe1 mouse
PROiPR:P06801
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032418 Expressed in 302 organ(s), highest expression level in adrenal gland
CleanExiMM_ME1
ExpressionAtlasiP06801 baseline and differential
GenevisibleiP06801 MM

Family and domain databases

Gene3Di3.40.50.10380, 1 hit
InterProiView protein in InterPro
IPR015884 Malic_enzyme_CS
IPR012301 Malic_N_dom
IPR037062 Malic_N_dom_sf
IPR012302 Malic_NAD-bd
IPR001891 Malic_OxRdtase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00390 malic, 1 hit
PF03949 Malic_M, 1 hit
PIRSFiPIRSF000106 ME, 1 hit
PRINTSiPR00072 MALOXRDTASE
SMARTiView protein in SMART
SM01274 malic, 1 hit
SM00919 Malic_M, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00331 MALIC_ENZYMES, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMAOX_MOUSE
AccessioniPrimary (citable) accession number: P06801
Secondary accession number(s): Q9DBF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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