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Protein

Cathepsin L1

Gene

Ctsl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Important for the overall degradation of proteins in lysosomes.

Catalytic activityi

Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei138By similarity1
Active sitei276By similarity1
Active sitei300By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14812
BRENDAi3.4.22.15 3474
ReactomeiR-MMU-1474228 Degradation of the extracellular matrix
R-MMU-1592389 Activation of Matrix Metalloproteinases
R-MMU-1679131 Trafficking and processing of endosomal TLR
R-MMU-2022090 Assembly of collagen fibrils and other multimeric structures
R-MMU-2132295 MHC class II antigen presentation
R-MMU-8939242 RUNX1 regulates transcription of genes involved in differentiation of keratinocytes

Protein family/group databases

MEROPSiC01.032

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin L1 (EC:3.4.22.15)
Alternative name(s):
Cathepsin L
Major excreted protein
Short name:
MEP
p39 cysteine proteinase
Cleaved into the following 2 chains:
Gene namesi
Name:Ctsl
Synonyms:Ctsl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:88564 Ctsl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5291

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
PropeptideiPRO_000002624818 – 113Activation peptideAdd BLAST96
ChainiPRO_0000026249114 – 288Cathepsin L1 heavy chainAdd BLAST175
PropeptideiPRO_0000026250289 – 2902
ChainiPRO_0000026251291 – 334Cathepsin L1 light chainAdd BLAST44

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi135 ↔ 178By similarity
Disulfide bondi169 ↔ 211By similarity
Glycosylationi221N-linked (GlcNAc...) (high mannose) asparagine2 Publications1
Disulfide bondi269 ↔ 322Interchain (between heavy and light chains)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP06797
PaxDbiP06797
PeptideAtlasiP06797
PRIDEiP06797

PTM databases

iPTMnetiP06797
PhosphoSitePlusiP06797
SwissPalmiP06797
UniCarbKBiP06797

Expressioni

Gene expression databases

BgeeiENSMUSG00000021477 Expressed in 294 organ(s), highest expression level in embryo
CleanExiMM_CTSL
ExpressionAtlasiP06797 baseline and differential
GenevisibleiP06797 MM

Interactioni

Subunit structurei

Dimer of a heavy and a light chain linked by disulfide bonds.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198975, 2 interactors
IntActiP06797, 5 interactors
MINTiP06797
STRINGi10090.ENSMUSP00000021933

Chemistry databases

BindingDBiP06797

Structurei

3D structure databases

ProteinModelPortaliP06797
SMRiP06797
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543 Eukaryota
COG4870 LUCA
GeneTreeiENSGT00900000140823
HOGENOMiHOG000230774
HOVERGENiHBG011513
InParanoidiP06797
KOiK01365
OMAiFEGANSD
OrthoDBiEOG091G0AKT
PhylomeDBiP06797
TreeFamiTF313739

Family and domain databases

CDDicd02248 Peptidase_C1A, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR039417 Peptidase_C1A_papain-like
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P06797-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNLLLLLAVL CLGTALATPK FDQTFSAEWH QWKSTHRRLY GTNEEEWRRA
60 70 80 90 100
IWEKNMRMIQ LHNGEYSNGQ HGFSMEMNAF GDMTNEEFRQ VVNGYRHQKH
110 120 130 140 150
KKGRLFQEPL MLKIPKSVDW REKGCVTPVK NQGQCGSCWA FSASGCLEGQ
160 170 180 190 200
MFLKTGKLIS LSEQNLVDCS HAQGNQGCNG GLMDFAFQYI KENGGLDSEE
210 220 230 240 250
SYPYEAKDGS CKYRAEFAVA NDTGFVDIPQ QEKALMKAVA TVGPISVAMD
260 270 280 290 300
ASHPSLQFYS SGIYYEPNCS SKNLDHGVLL VGYGYEGTDS NKNKYWLVKN
310 320 330
SWGSEWGMEG YIKIAKDRDN HCGLATAASY PVVN
Length:334
Mass (Da):37,547
Last modified:November 1, 1990 - v2
Checksum:iFE6747043307AD98
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1Y7VNQ4A0A1Y7VNQ4_MOUSE
Cathepsin L1
Ctsl
163Annotation score:
A0A1Y7VNM3A0A1Y7VNM3_MOUSE
Cathepsin L1
Ctsl
205Annotation score:
A0A1Y7VJC1A0A1Y7VJC1_MOUSE
Cathepsin L1
Ctsl
94Annotation score:
A0A1Y7VP49A0A1Y7VP49_MOUSE
Cathepsin L1
Ctsl
157Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58M → I in AAA39984 (PubMed:2835398).Curated1
Sequence conflicti58M → I in AAD32136 (PubMed:2835398).Curated1
Sequence conflicti58M → I in AAD32137 (PubMed:2835398).Curated1
Sequence conflicti58M → I in AAD32138 (PubMed:2835398).Curated1
Sequence conflicti177G → R (PubMed:3533924).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06086 mRNA Translation: CAA29470.1
J02583 mRNA Translation: AAA37445.1
M20495 Genomic DNA Translation: AAA39984.1
AF121837 mRNA Translation: AAD32136.1
AF121838 mRNA Translation: AAD32137.1
AF121839 mRNA Translation: AAD32138.1
BC068163 mRNA Translation: AAH68163.1
X04392 mRNA Translation: CAA27980.1
CCDSiCCDS26600.1
PIRiS01177 KHMSL
RefSeqiNP_034114.1, NM_009984.4
XP_006517143.1, XM_006517080.1
UniGeneiMm.471755
Mm.930

Genome annotation databases

EnsembliENSMUST00000021933; ENSMUSP00000021933; ENSMUSG00000021477
ENSMUST00000222517; ENSMUSP00000152169; ENSMUSG00000021477
GeneIDi13039
KEGGimmu:13039
UCSCiuc007qyw.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06086 mRNA Translation: CAA29470.1
J02583 mRNA Translation: AAA37445.1
M20495 Genomic DNA Translation: AAA39984.1
AF121837 mRNA Translation: AAD32136.1
AF121838 mRNA Translation: AAD32137.1
AF121839 mRNA Translation: AAD32138.1
BC068163 mRNA Translation: AAH68163.1
X04392 mRNA Translation: CAA27980.1
CCDSiCCDS26600.1
PIRiS01177 KHMSL
RefSeqiNP_034114.1, NM_009984.4
XP_006517143.1, XM_006517080.1
UniGeneiMm.471755
Mm.930

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MVVmodel-A22-334[»]
ProteinModelPortaliP06797
SMRiP06797
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198975, 2 interactors
IntActiP06797, 5 interactors
MINTiP06797
STRINGi10090.ENSMUSP00000021933

Chemistry databases

BindingDBiP06797
ChEMBLiCHEMBL5291

Protein family/group databases

MEROPSiC01.032

PTM databases

iPTMnetiP06797
PhosphoSitePlusiP06797
SwissPalmiP06797
UniCarbKBiP06797

Proteomic databases

EPDiP06797
PaxDbiP06797
PeptideAtlasiP06797
PRIDEiP06797

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021933; ENSMUSP00000021933; ENSMUSG00000021477
ENSMUST00000222517; ENSMUSP00000152169; ENSMUSG00000021477
GeneIDi13039
KEGGimmu:13039
UCSCiuc007qyw.1 mouse

Organism-specific databases

CTDi1514
MGIiMGI:88564 Ctsl

Phylogenomic databases

eggNOGiKOG1543 Eukaryota
COG4870 LUCA
GeneTreeiENSGT00900000140823
HOGENOMiHOG000230774
HOVERGENiHBG011513
InParanoidiP06797
KOiK01365
OMAiFEGANSD
OrthoDBiEOG091G0AKT
PhylomeDBiP06797
TreeFamiTF313739

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14812
BRENDAi3.4.22.15 3474
ReactomeiR-MMU-1474228 Degradation of the extracellular matrix
R-MMU-1592389 Activation of Matrix Metalloproteinases
R-MMU-1679131 Trafficking and processing of endosomal TLR
R-MMU-2022090 Assembly of collagen fibrils and other multimeric structures
R-MMU-2132295 MHC class II antigen presentation
R-MMU-8939242 RUNX1 regulates transcription of genes involved in differentiation of keratinocytes

Miscellaneous databases

ChiTaRSiCtsl mouse
PROiPR:P06797
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021477 Expressed in 294 organ(s), highest expression level in embryo
CleanExiMM_CTSL
ExpressionAtlasiP06797 baseline and differential
GenevisibleiP06797 MM

Family and domain databases

CDDicd02248 Peptidase_C1A, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR039417 Peptidase_C1A_papain-like
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCATL1_MOUSE
AccessioniPrimary (citable) accession number: P06797
Secondary accession number(s): Q91UZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1990
Last modified: November 7, 2018
This is version 187 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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