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Protein

DNA repair and recombination protein RAD52

Gene

RAD52

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in DNA double-strand break (DSB) repair and recombination. Promotes the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.

Miscellaneous

Present with 1080 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA/DNA annealing activity Source: SGD
  • recombinase activity Source: SGD

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA recombination, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-32633-MONOMER
ReactomeiR-SCE-3108214 SUMOylation of DNA damage response and repair proteins
R-SCE-5685938 HDR through Single Strand Annealing (SSA)

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair and recombination protein RAD52
Gene namesi
Name:RAD52
Ordered Locus Names:YML032C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML032C
SGDiS000004494 RAD52

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738921 – 471DNA repair and recombination protein RAD52Add BLAST471

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP06778
PRIDEiP06778

PTM databases

iPTMnetiP06778

Interactioni

Subunit structurei

Part of a complex that includes RAD51, RAD52 and RAD59. Interacts with SAW1.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi35137, 564 interactors
DIPiDIP-3N
IntActiP06778, 32 interactors
MINTiP06778
STRINGi4932.YML032C

Structurei

3D structure databases

ProteinModelPortaliP06778
SMRiP06778
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RAD52 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000008766
HOGENOMiHOG000154067
InParanoidiP06778
KOiK10873
OMAiCLRCFGN
OrthoDBiEOG092C4C2A

Family and domain databases

InterProiView protein in InterPro
IPR004585 DNA_recomb/repair_Rad52
IPR007232 Rad52_Rad59_Rad22
PANTHERiPTHR12132 PTHR12132, 1 hit
PfamiView protein in Pfam
PF04098 Rad52_Rad22, 1 hit
TIGRFAMsiTIGR00607 rad52, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket
Isoform 1 (identifier: P06778-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNEIMDMDEK KPVFGNHSED IQTKLDKKLG PEYISKRVGF GTSRIAYIEG
60 70 80 90 100
WRVINLANQI FGYNGWSTEV KSVVIDFLDE RQGKFSIGCT AIVRVTLTSG
110 120 130 140 150
TYREDIGYGT VENERRKPAA FERAKKSAVT DALKRSLRGF GNALGNCLYD
160 170 180 190 200
KDFLAKIDKV KFDPPDFDEN NLFRPTDEIS ESSRTNTLHE NQEQQQYPNK
210 220 230 240 250
RRQLTKVTNT NPDSTKNLVK IENTVSRGTP MMAAPAEANS KNSSNKDTDL
260 270 280 290 300
KSLDASKQDQ DDLLDDSLMF SDDFQDDDLI NMGNTNSNVL TTEKDPVVAK
310 320 330 340 350
QSPTASSNPE AEQITFVTAK AATSVQNERY IGEESIFDPK YQAQSIRHTV
360 370 380 390 400
DQTTSKHIPA SVLKDKTMTT ARDSVYEKFA PKGKQLSMKN NDKELGPHML
410 420 430 440 450
EGAGNQVPRE TTPIKTNATA FPPAAAPRFA PPSKVVHPNG NGAVPAVPQQ
460 470
RSTRREVGRP KINPLHARKP T
Length:471
Mass (Da):52,404
Last modified:June 27, 2006 - v2
Checksum:i4F5D07480A05B564
GO
Isoform 2 (identifier: P06778-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.

Note: Produced by alternative initiation at Met-5 of isoform 1.
Show »
Length:467
Mass (Da):51,916
Checksum:i857A5AD7AA014748
GO
Isoform 3 (identifier: P06778-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Note: Produced by alternative initiation at Met-7 of isoform 1.
Show »
Length:465
Mass (Da):51,670
Checksum:i426DFDCAB969A5A4
GO

Sequence cautioni

The sequence AAA50352 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAT93163 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA86623 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0196131 – 6Missing in isoform 3. Curated6
Alternative sequenceiVSP_0196121 – 4Missing in isoform 2. Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10249 Genomic DNA Translation: AAA50352.1 Different initiation.
Z46659 Genomic DNA Translation: CAA86623.1 Different initiation.
AY693144 Genomic DNA Translation: AAT93163.1 Different initiation.
BK006946 Genomic DNA Translation: DAA09866.1
PIRiA23282
RefSeqiNP_013680.2, NM_001182390.1 [P06778-1]

Genome annotation databases

EnsemblFungiiYML032C; YML032C; YML032C [P06778-1]
GeneIDi854976
KEGGisce:YML032C

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10249 Genomic DNA Translation: AAA50352.1 Different initiation.
Z46659 Genomic DNA Translation: CAA86623.1 Different initiation.
AY693144 Genomic DNA Translation: AAT93163.1 Different initiation.
BK006946 Genomic DNA Translation: DAA09866.1
PIRiA23282
RefSeqiNP_013680.2, NM_001182390.1 [P06778-1]

3D structure databases

ProteinModelPortaliP06778
SMRiP06778
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35137, 564 interactors
DIPiDIP-3N
IntActiP06778, 32 interactors
MINTiP06778
STRINGi4932.YML032C

PTM databases

iPTMnetiP06778

Proteomic databases

PaxDbiP06778
PRIDEiP06778

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML032C; YML032C; YML032C [P06778-1]
GeneIDi854976
KEGGisce:YML032C

Organism-specific databases

EuPathDBiFungiDB:YML032C
SGDiS000004494 RAD52

Phylogenomic databases

GeneTreeiENSGT00390000008766
HOGENOMiHOG000154067
InParanoidiP06778
KOiK10873
OMAiCLRCFGN
OrthoDBiEOG092C4C2A

Enzyme and pathway databases

BioCyciYEAST:G3O-32633-MONOMER
ReactomeiR-SCE-3108214 SUMOylation of DNA damage response and repair proteins
R-SCE-5685938 HDR through Single Strand Annealing (SSA)

Miscellaneous databases

PROiPR:P06778

Family and domain databases

InterProiView protein in InterPro
IPR004585 DNA_recomb/repair_Rad52
IPR007232 Rad52_Rad59_Rad22
PANTHERiPTHR12132 PTHR12132, 1 hit
PfamiView protein in Pfam
PF04098 Rad52_Rad22, 1 hit
TIGRFAMsiTIGR00607 rad52, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAD52_YEAST
AccessioniPrimary (citable) accession number: P06778
Secondary accession number(s): D6VZE2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: June 27, 2006
Last modified: September 12, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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