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Protein

DNA repair protein RAD1

Gene

RAD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in nucleotide excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Along with RAD10 forms an endonuclease that specifically degrades single-stranded DNA.1 Publication

Miscellaneous

Present with 1400 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • damaged DNA binding Source: GO_Central
  • single-stranded DNA binding Source: SGD
  • single-stranded DNA endodeoxyribonuclease activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Endonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-33940-MONOMER
ReactomeiR-SCE-5696395 Formation of Incision Complex in GG-NER
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD1
Gene namesi
Name:RAD1
Ordered Locus Names:YPL022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

SGDiS000005943 RAD1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988571 – 1100DNA repair protein RAD1Add BLAST1100

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei613PhosphoserineCombined sources1
Modified residuei1071PhosphoserineCombined sources1
Modified residuei1072PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06777
PaxDbiP06777
PRIDEiP06777

PTM databases

iPTMnetiP06777

Interactioni

Subunit structurei

Component of the nucleotide excision repair factor 1 (NEF1) complex consisting of RAD1, RAD10 and RAD14. Interacts with SAW1.4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi36155, 483 interactors
ComplexPortaliCPX-1362 SLX4-RAD1-RAD10 endonuclease complex
CPX-1363 SAW1-RAD1-RAD10 endonuclease complex
CPX-1708 Nucleotide-excision repair factor 1 complex
DIPiDIP-2424N
IntActiP06777, 46 interactors
MINTiP06777
STRINGi4932.YPL022W

Structurei

3D structure databases

ProteinModelPortaliP06777
SMRiP06777
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini821 – 901ERCC4Add BLAST81

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 110Asp/Glu-rich (acidic)Add BLAST110
Compositional biasi516 – 576Arg/Lys-rich (basic)Add BLAST61
Compositional biasi595 – 689Asp/Glu-rich (acidic)Add BLAST95
Compositional biasi1041 – 1100Asp/Glu-rich (acidic)Add BLAST60

Sequence similaritiesi

Belongs to the XPF family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000004394
HOGENOMiHOG000202204
InParanoidiP06777
KOiK10848
OMAiVENCVTK
OrthoDBiEOG092C0B3U

Family and domain databases

InterProiView protein in InterPro
IPR006166 ERCC4_domain
IPR011335 Restrct_endonuc-II-like
IPR010994 RuvA_2-like
IPR006167 XPF
PfamiView protein in Pfam
PF02732 ERCC4, 1 hit
SMARTiView protein in SMART
SM00891 ERCC4, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF52980 SSF52980, 1 hit
TIGRFAMsiTIGR00596 rad1, 1 hit

Sequencei

Sequence statusi: Complete.

P06777-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSQLFYQGDS DDELQEELTR QTTQASQSSK IKNEDEPDDS NHLNEVENED
60 70 80 90 100
SKVLDDDAVL YPLIPNEPDD IETSKPNIND IRPVDIQLTL PLPFQQKVVE
110 120 130 140 150
NSLITEDALI IMGKGLGLLD IVANLLHVLA TPTSINGQLK RALVLVLNAK
160 170 180 190 200
PIDNVRIKEA LEELSWFSNT GKDDDDTAVE SDDELFERPF NVVTADSLSI
210 220 230 240 250
EKRRKLYISG GILSITSRIL IVDLLSGIVH PNRVTGMLVL NADSLRHNSN
260 270 280 290 300
ESFILEIYRS KNTWGFIKAF SEAPETFVME FSPLRTKMKE LRLKNVLLWP
310 320 330 340 350
RFRVEVSSCL NATNKTSHNK VIEVKVSLTN SMSQIQFGLM ECLKKCIAEL
360 370 380 390 400
SRKNPELALD WWNMENVLDI NFIRSIDSVM VPNWHRISYE SKQLVKDIRF
410 420 430 440 450
LRHLLKMLVT SDAVDFFGEI QLSLDANKPS VSRKYSESPW LLVDEAQLVI
460 470 480 490 500
SYAKKRIFYK NEYTLEENPK WEQLIHILHD ISHERMTNHL QGPTLVACSD
510 520 530 540 550
NLTCLELAKV LNASNKKRGV RQVLLNKLKW YRKQREETKK LVKEVQSQDT
560 570 580 590 600
FPENATLNVS STFSKEQVTT KRRRTRGASQ VAAVEKLRNA GTNVDMEVVF
610 620 630 640 650
EDHKLSEEIK KGSGDDLDDG QEENAANDSK IFEIQEQENE ILIDDGDAEF
660 670 680 690 700
DNGELEYVGD LPQHITTHFN KDLWAEHCNE YEYVDRQDEI LISTFKSLND
710 720 730 740 750
NCSLQEMMPS YIIMFEPDIS FIRQIEVYKA IVKDLQPKVY FMYYGESIEE
760 770 780 790 800
QSHLTAIKRE KDAFTKLIRE NANLSHHFET NEDLSHYKNL AERKLKLSKL
810 820 830 840 850
RKSNTRNAGG QQGFHNLTQD VVIVDTREFN ASLPGLLYRY GIRVIPCMLT
860 870 880 890 900
VGDYVITPDI CLERKSISDL IGSLQNNRLA NQCKKMLKYY AYPTLLIEFD
910 920 930 940 950
EGQSFSLEPF SERRNYKNKD ISTVHPISSK LSQDEIQLKL AKLVLRFPTL
960 970 980 990 1000
KIIWSSSPLQ TVNIILELKL GREQPDPSNA VILGTNKVRS DFNSTAKGLK
1010 1020 1030 1040 1050
DGDNESKFKR LLNVPGVSKI DYFNLRKKIK SFNKLQKLSW NEINELINDE
1060 1070 1080 1090 1100
DLTDRIYYFL RTEKEEQEQE STDENLESPG KTTDDNALHD HHNDVPEAPV
Length:1,100
Mass (Da):126,370
Last modified:January 1, 1988 - v1
Checksum:i06FDA601F3F59B10
GO

Sequence cautioni

The sequence AAA34929 differs from that shown. Reason: Frameshift at positions 923, 934 and 948.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223D → N in AAA34929 (PubMed:6095044).Curated1
Sequence conflicti883C → Y in AAA34929 (PubMed:6095044).Curated1
Sequence conflicti886M → I in AAA34929 (PubMed:6095044).Curated1
Sequence conflicti912E → K in AAA34929 (PubMed:6095044).Curated1
Sequence conflicti924V → I in AAA34929 (PubMed:6095044).Curated1
Sequence conflicti1016G → R in AAA34929 (PubMed:6095044).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15435 Genomic DNA Translation: AAA34934.1
U36624 Genomic DNA Translation: AAB68165.1
K02070 Genomic DNA Translation: AAA34929.1 Frameshift.
BK006949 Genomic DNA Translation: DAA11406.1
PIRiA26129 DDBYD1
RefSeqiNP_015303.1, NM_001183836.1

Genome annotation databases

EnsemblFungiiYPL022W; YPL022W; YPL022W
GeneIDi856085
KEGGisce:YPL022W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15435 Genomic DNA Translation: AAA34934.1
U36624 Genomic DNA Translation: AAB68165.1
K02070 Genomic DNA Translation: AAA34929.1 Frameshift.
BK006949 Genomic DNA Translation: DAA11406.1
PIRiA26129 DDBYD1
RefSeqiNP_015303.1, NM_001183836.1

3D structure databases

ProteinModelPortaliP06777
SMRiP06777
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36155, 483 interactors
ComplexPortaliCPX-1362 SLX4-RAD1-RAD10 endonuclease complex
CPX-1363 SAW1-RAD1-RAD10 endonuclease complex
CPX-1708 Nucleotide-excision repair factor 1 complex
DIPiDIP-2424N
IntActiP06777, 46 interactors
MINTiP06777
STRINGi4932.YPL022W

PTM databases

iPTMnetiP06777

Proteomic databases

MaxQBiP06777
PaxDbiP06777
PRIDEiP06777

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL022W; YPL022W; YPL022W
GeneIDi856085
KEGGisce:YPL022W

Organism-specific databases

SGDiS000005943 RAD1

Phylogenomic databases

GeneTreeiENSGT00390000004394
HOGENOMiHOG000202204
InParanoidiP06777
KOiK10848
OMAiVENCVTK
OrthoDBiEOG092C0B3U

Enzyme and pathway databases

BioCyciYEAST:G3O-33940-MONOMER
ReactomeiR-SCE-5696395 Formation of Incision Complex in GG-NER
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER

Miscellaneous databases

PROiPR:P06777

Family and domain databases

InterProiView protein in InterPro
IPR006166 ERCC4_domain
IPR011335 Restrct_endonuc-II-like
IPR010994 RuvA_2-like
IPR006167 XPF
PfamiView protein in Pfam
PF02732 ERCC4, 1 hit
SMARTiView protein in SMART
SM00891 ERCC4, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF52980 SSF52980, 1 hit
TIGRFAMsiTIGR00596 rad1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAD1_YEAST
AccessioniPrimary (citable) accession number: P06777
Secondary accession number(s): D6W3Z0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 7, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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