UniProtKB - P06776 (PDE2_YEAST)
Protein
3',5'-cyclic-nucleotide phosphodiesterase 2
Gene
PDE2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Controls the level of cAMP in yeast cells, together with the low-affinity cAMP phosphodiesterase (PDE1).
Miscellaneous
Present with 6510 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:3.1.4.53
Cofactori
a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 265 | Proton donorBy similarity | 1 | |
Metal bindingi | 269 | Divalent metal cation 1By similarity | 1 | |
Metal bindingi | 302 | Divalent metal cation 1By similarity | 1 | |
Metal bindingi | 303 | Divalent metal cation 1By similarity | 1 | |
Metal bindingi | 303 | Divalent metal cation 2By similarity | 1 | |
Metal bindingi | 400 | Divalent metal cation 1By similarity | 1 |
GO - Molecular functioni
- 3',5'-cyclic-AMP phosphodiesterase activity Source: SGD
- 3',5'-cyclic-nucleotide phosphodiesterase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cAMP-mediated signaling Source: SGD
- signal transduction Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Ligand | cAMP, Metal-binding |
Enzyme and pathway databases
Reactomei | R-SCE-418457, cGMP effects R-SCE-418555, G alpha (s) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: 3',5'-cyclic-nucleotide phosphodiesterase 2 (EC:3.1.4.53)Short name: PDEase 2 Alternative name(s): High-affinity cAMP phosphodiesterase |
Gene namesi | Name:PDE2 Synonyms:SRA5 Ordered Locus Names:YOR360C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005887, PDE2 |
VEuPathDBi | FungiDB:YOR360C |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198851 | 1 – 526 | 3',5'-cyclic-nucleotide phosphodiesterase 2Add BLAST | 526 |
Proteomic databases
MaxQBi | P06776 |
PaxDbi | P06776 |
PRIDEi | P06776 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 34745, 631 interactors |
DIPi | DIP-4055N |
IntActi | P06776, 3 interactors |
MINTi | P06776 |
STRINGi | 4932.YOR360C |
Miscellaneous databases
RNActi | P06776, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 182 – 526 | PDEasePROSITE-ProRule annotationAdd BLAST | 345 |
Sequence similaritiesi
Belongs to the cyclic nucleotide phosphodiesterase family.Curated
Phylogenomic databases
eggNOGi | KOG3689, Eukaryota |
GeneTreei | ENSGT00960000189193 |
HOGENOMi | CLU_028903_0_0_1 |
OMAi | QIFFINT |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
Gene3Di | 1.10.1300.10, 1 hit |
InterProi | View protein in InterPro IPR003607, HD/PDEase_dom IPR023088, PDEase IPR002073, PDEase_catalytic_dom IPR036971, PDEase_catalytic_dom_sf IPR023174, PDEase_CS |
Pfami | View protein in Pfam PF00233, PDEase_I, 1 hit |
PRINTSi | PR00387, PDIESTERASE1 |
SMARTi | View protein in SMART SM00471, HDc, 1 hit |
PROSITEi | View protein in PROSITE PS00126, PDEASE_I_1, 1 hit PS51845, PDEASE_I_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P06776-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTLFLIGIH EIEKSQTIVQ NEHYFDRVIE LQDLDSLMVA LYKDRVSPFP
60 70 80 90 100
NVHNFETGVS IVLYDPSKFQ LSVRQLDVLF KRFFPSFNIS AIDHTREENL
110 120 130 140 150
QRLECVEREN SICRNRITRI NHWMYHHHDD TPDGINKNSY GTVNGNSVPT
160 170 180 190 200
QACEANIYTL LLHLNDSKAQ HLRKASVPRL IRNIEFMSFL SDPIEKISQE
210 220 230 240 250
GSHYWNILST WDFCALSLST QELIWCGFTL IKKLSKDAKV LIADNKLLLL
260 270 280 290 300
LFTLESSYHQ VNKFHNFRHA IDVMQATWRL CTYLLKDNPV QTLLLCMAAI
310 320 330 340 350
GHDVGHPGTN NQLLCNCESE VAQNFKNVSI LENFHRELFQ QLLSEHWPQL
360 370 380 390 400
LSISKKKFDF ISEAILATDM ALHSQYEDRL MHENPMKQIT LISLIIKAAD
410 420 430 440 450
ISNVTRTLSI SARWAYLITL EFNDCALLET FHKAHRPEQD CFGDSYKNVD
460 470 480 490 500
SPKEDLESIQ NILVNVTDPD DIIKDHPHIP NGQIFFINTF AEVFFNALSQ
510 520
KFSGLKFLSD NVKINKEYWM KHKKPQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 82 | R → G in AAA34846 (PubMed:3025832).Curated | 1 | |
Sequence conflicti | 349 – 350 | QL → LK in AAA34846 (PubMed:3025832).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14563 Genomic DNA Translation: AAA34846.1 Z75268 Genomic DNA Translation: CAA99689.1 BK006948 Genomic DNA Translation: DAA11121.1 |
PIRi | S67272 |
RefSeqi | NP_015005.1, NM_001183780.1 |
Genome annotation databases
EnsemblFungii | YOR360C_mRNA; YOR360C; YOR360C |
GeneIDi | 854542 |
KEGGi | sce:YOR360C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14563 Genomic DNA Translation: AAA34846.1 Z75268 Genomic DNA Translation: CAA99689.1 BK006948 Genomic DNA Translation: DAA11121.1 |
PIRi | S67272 |
RefSeqi | NP_015005.1, NM_001183780.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 34745, 631 interactors |
DIPi | DIP-4055N |
IntActi | P06776, 3 interactors |
MINTi | P06776 |
STRINGi | 4932.YOR360C |
Proteomic databases
MaxQBi | P06776 |
PaxDbi | P06776 |
PRIDEi | P06776 |
Genome annotation databases
EnsemblFungii | YOR360C_mRNA; YOR360C; YOR360C |
GeneIDi | 854542 |
KEGGi | sce:YOR360C |
Organism-specific databases
SGDi | S000005887, PDE2 |
VEuPathDBi | FungiDB:YOR360C |
Phylogenomic databases
eggNOGi | KOG3689, Eukaryota |
GeneTreei | ENSGT00960000189193 |
HOGENOMi | CLU_028903_0_0_1 |
OMAi | QIFFINT |
Enzyme and pathway databases
Reactomei | R-SCE-418457, cGMP effects R-SCE-418555, G alpha (s) signalling events |
Miscellaneous databases
PROi | PR:P06776 |
RNActi | P06776, protein |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
Gene3Di | 1.10.1300.10, 1 hit |
InterProi | View protein in InterPro IPR003607, HD/PDEase_dom IPR023088, PDEase IPR002073, PDEase_catalytic_dom IPR036971, PDEase_catalytic_dom_sf IPR023174, PDEase_CS |
Pfami | View protein in Pfam PF00233, PDEase_I, 1 hit |
PRINTSi | PR00387, PDIESTERASE1 |
SMARTi | View protein in SMART SM00471, HDc, 1 hit |
PROSITEi | View protein in PROSITE PS00126, PDEASE_I_1, 1 hit PS51845, PDEASE_I_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDE2_YEAST | |
Accessioni | P06776Primary (citable) accession number: P06776 Secondary accession number(s): D6W355, Q08836 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - SIMILARITY comments
Index of protein domains and families - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names