UniProtKB - P06774 (HAP2_YEAST)
Transcriptional activator HAP2
HAP2
Functioni
Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'. HAP2 has primarily a structural role in the HAP complexes I and II.
1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 192 – 217 | NFYA/HAP2-typePROSITE-ProRule annotationAdd BLAST | 26 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
GO - Biological processi
- carbon catabolite activation of transcription from RNA polymerase II promoter Source: SGD
- regulation of carbohydrate metabolic process Source: ComplexPortal
- regulation of cellular respiration Source: SGD
- regulation of transcription, DNA-templated Source: ComplexPortal
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional activator HAP2 |
Gene namesi | Name:HAP2 Ordered Locus Names:YGL237C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003206, HAP2 |
VEuPathDBi | FungiDB:YGL237C |
Subcellular locationi
Nucleus
Nucleus
- CCAAT-binding factor complex Source: SGD
- nucleus Source: SGD
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 199 | R → L: Favors CCACC binding over CCAAT. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000198781 | 2 – 265 | Transcriptional activator HAP2Add BLAST | 264 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 52 | PhosphoserineCombined sources | 1 | |
Modified residuei | 265 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P06774 |
PaxDbi | P06774 |
PRIDEi | P06774 |
PTM databases
iPTMneti | P06774 |
Interactioni
Subunit structurei
Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4.
1 PublicationBinary interactionsi
P06774
With | #Exp. | IntAct |
---|---|---|
HAP5 [Q02516] | 7 | EBI-8152,EBI-8165 |
Protein-protein interaction databases
BioGRIDi | 33002, 173 interactors |
ComplexPortali | CPX-1830, CCAAT-binding factor complex |
DIPi | DIP-1361N |
IntActi | P06774, 142 interactors |
MINTi | P06774 |
STRINGi | 4932.YGL237C |
Miscellaneous databases
RNActi | P06774, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 64 | DisorderedSequence analysisAdd BLAST | 64 | |
Regioni | 23 – 180 | Interaction with the HAP2-HAP3-HAP5 heteromerAdd BLAST | 158 | |
Regioni | 78 – 129 | DisorderedSequence analysisAdd BLAST | 52 | |
Regioni | 220 – 265 | DisorderedSequence analysisAdd BLAST | 46 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 162 – 185 | Subunit association domain (SAD)Add BLAST | 24 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 18 – 34 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 225 – 247 | Basic and acidic residuesSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1561, Eukaryota |
GeneTreei | ENSGT00390000015714 |
HOGENOMi | CLU_1046461_0_0_1 |
InParanoidi | P06774 |
OMAi | QYHRIMV |
Family and domain databases
InterProi | View protein in InterPro IPR018362, CCAAT-binding_factor_CS IPR001289, NFYA |
PANTHERi | PTHR12632, PTHR12632, 1 hit |
Pfami | View protein in Pfam PF02045, CBFB_NFYA, 1 hit |
PRINTSi | PR00616, CCAATSUBUNTB |
SMARTi | View protein in SMART SM00521, CBF, 1 hit |
PROSITEi | View protein in PROSITE PS00686, NFYA_HAP2_1, 1 hit PS51152, NFYA_HAP2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSADETDAKF HPLETDLQSD TAAATSTAAA SRSPSLQEKP IEMPLDMGKA
60 70 80 90 100
PSPRGEDQRV TNEEDLFLFN RLRASQNRVM DSLEPQQQSQ YTSSSVSTME
110 120 130 140 150
PSADFTSFSA VTTLPPPPHQ QQQQQQQQQQ QQQLVVQAQY TQNQPNLQSD
160 170 180 190 200
VLGTAIAEQP FYVNAKQYYR ILKRRYARAK LEEKLRISRE RKPYLHESRH
210 220 230 240 250
KHAMRRPRGE GGRFLTAAEI KAMKSKKSGA SDDPDDSHED KKITTKIIQE
260
QPHATSTAAA ADKKT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15243 Genomic DNA Translation: AAA34663.1 Z72759 Genomic DNA Translation: CAA96955.1 BK006941 Genomic DNA Translation: DAA07881.1 |
PIRi | A26771 |
RefSeqi | NP_011277.1, NM_001181103.1 |
Genome annotation databases
EnsemblFungii | YGL237C_mRNA; YGL237C; YGL237C |
GeneIDi | 852614 |
KEGGi | sce:YGL237C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15243 Genomic DNA Translation: AAA34663.1 Z72759 Genomic DNA Translation: CAA96955.1 BK006941 Genomic DNA Translation: DAA07881.1 |
PIRi | A26771 |
RefSeqi | NP_011277.1, NM_001181103.1 |
3D structure databases
AlphaFoldDBi | P06774 |
SMRi | P06774 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33002, 173 interactors |
ComplexPortali | CPX-1830, CCAAT-binding factor complex |
DIPi | DIP-1361N |
IntActi | P06774, 142 interactors |
MINTi | P06774 |
STRINGi | 4932.YGL237C |
PTM databases
iPTMneti | P06774 |
Proteomic databases
MaxQBi | P06774 |
PaxDbi | P06774 |
PRIDEi | P06774 |
Genome annotation databases
EnsemblFungii | YGL237C_mRNA; YGL237C; YGL237C |
GeneIDi | 852614 |
KEGGi | sce:YGL237C |
Organism-specific databases
SGDi | S000003206, HAP2 |
VEuPathDBi | FungiDB:YGL237C |
Phylogenomic databases
eggNOGi | KOG1561, Eukaryota |
GeneTreei | ENSGT00390000015714 |
HOGENOMi | CLU_1046461_0_0_1 |
InParanoidi | P06774 |
OMAi | QYHRIMV |
Miscellaneous databases
PROi | PR:P06774 |
RNActi | P06774, protein |
Family and domain databases
InterProi | View protein in InterPro IPR018362, CCAAT-binding_factor_CS IPR001289, NFYA |
PANTHERi | PTHR12632, PTHR12632, 1 hit |
Pfami | View protein in Pfam PF02045, CBFB_NFYA, 1 hit |
PRINTSi | PR00616, CCAATSUBUNTB |
SMARTi | View protein in SMART SM00521, CBF, 1 hit |
PROSITEi | View protein in PROSITE PS00686, NFYA_HAP2_1, 1 hit PS51152, NFYA_HAP2_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HAP2_YEAST | |
Accessioni | P06774Primary (citable) accession number: P06774 Secondary accession number(s): D6VV97 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | May 25, 2022 | |
This is version 185 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - SIMILARITY comments
Index of protein domains and families