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UniProtKB - P06750 (AGGL_RICCO)
Protein
Agglutinin
Gene
N/A
Organism
Ricinus communis (Castor bean)
Status
Functioni
Catalytic activityi
- Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. EC:3.2.2.22
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 104 | By similarity | 1 | |
Active sitei | 147 | By similarity | 1 | |
Active sitei | 200 | By similarity | 1 | |
Active sitei | 203 | By similarity | 1 | |
Binding sitei | 312 | Beta-D-galactose 3; via carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 337 | Beta-D-galactose 2Combined sources1 Publication | 1 | |
Binding sitei | 342 | Beta-D-galactose 2Combined sources1 Publication | 1 | |
Binding sitei | 348 | Beta-D-galactose 2Combined sources1 Publication | 1 | |
Binding sitei | 437 | Beta-D-galactose 3Combined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 104 – 105 | AMPBy similarity | 2 | |
Nucleotide bindingi | 145 – 147 | AMPBy similarity | 3 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- nucleotide binding Source: UniProtKB-KW
- rRNA N-glycosylase activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- defense response Source: UniProtKB-KW
- negative regulation of translation Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protein synthesis inhibitor, Toxin |
Biological process | Plant defense |
Ligand | Lectin, Nucleotide-binding |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 |
UniLectini | P06750 |
Names & Taxonomyi
Protein namesi | Recommended name: AgglutininAlternative name(s): RCA Cleaved into the following 2 chains: |
Organismi | Ricinus communis (Castor bean) |
Taxonomic identifieri | 3988 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Malpighiales › Euphorbiaceae › Acalyphoideae › Acalypheae › Ricinus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
ChainiPRO_0000030726 | 25 – 290 | Agglutinin A chainAdd BLAST | 266 | |
PropeptideiPRO_0000030727 | 291 – 302 | Linker peptide2 PublicationsAdd BLAST | 12 | |
ChainiPRO_0000030728 | 303 – 564 | Agglutinin B chain1 PublicationAdd BLAST | 262 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 34 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 259 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 282 ↔ 306 | Interchain (between A and B chains)PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 322 ↔ 341 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 365 ↔ 382 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 397 | N-linked (GlcNAc...) asparagine | 1 | |
Glycosylationi | 437 | N-linked (GlcNAc...) asparagine | 1 | |
Disulfide bondi | 453 ↔ 466 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 492 ↔ 509 | PROSITE-ProRule annotation1 Publication |
Keywords - PTMi
Disulfide bond, GlycoproteinPTM databases
GlyConnecti | 10, 3 N-Linked glycans |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P06750 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06750 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 309 – 436 | Ricin B-type lectin 1PROSITE-ProRule annotationAdd BLAST | 128 | |
Repeati | 319 – 361 | 1-alphaAdd BLAST | 43 | |
Repeati | 362 – 402 | 1-betaAdd BLAST | 41 | |
Repeati | 405 – 437 | 1-gammaAdd BLAST | 33 | |
Domaini | 439 – 563 | Ricin B-type lectin 2PROSITE-ProRule annotationAdd BLAST | 125 | |
Repeati | 450 – 485 | 2-alphaAdd BLAST | 36 | |
Repeati | 489 – 528 | 2-betaAdd BLAST | 40 | |
Repeati | 531 – 558 | 2-gammaAdd BLAST | 28 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 32 – 34 | Beta-D-galactose 1 bindingCombined sources1 Publication | 3 | |
Regioni | 324 – 328 | Beta-D-galactose 2 bindingCombined sources1 Publication | 5 |
Sequence similaritiesi
In the N-terminal section; belongs to the ribosome-inactivating protein family. Type 2 RIP subfamily.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502QUVN, Eukaryota |
Family and domain databases
CDDi | cd00161, RICIN, 2 hits |
Gene3Di | 3.40.420.10, 1 hit 4.10.470.10, 1 hit |
InterProi | View protein in InterPro IPR036041, Ribosome-inact_prot_sf IPR017989, Ribosome_inactivat_1/2 IPR001574, Ribosome_inactivat_prot IPR017988, Ribosome_inactivat_prot_CS IPR016138, Ribosome_inactivat_prot_sub1 IPR016139, Ribosome_inactivat_prot_sub2 IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
PANTHERi | PTHR33453, PTHR33453, 1 hit |
Pfami | View protein in Pfam PF00652, Ricin_B_lectin, 2 hits PF00161, RIP, 1 hit |
PRINTSi | PR00396, SHIGARICIN |
SMARTi | View protein in SMART SM00458, RICIN, 2 hits |
SUPFAMi | SSF50370, SSF50370, 2 hits SSF56371, SSF56371, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 2 hits PS00275, SHIGA_RICIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P06750-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYAVATWLCF GSTSGWSFTL EDNNIFPKQY PIINFTTADA TVESYTNFIR
60 70 80 90 100
AVRSHLTTGA DVRHEIPVLP NRVGLPISQR FILVELSNHA ELSVTLALDV
110 120 130 140 150
TNAYVVGCRA GNSAYFFHPD NQEDAEAITH LFTDVQNSFT FAFGGNYDRL
160 170 180 190 200
EQLGGLRENI ELGTGPLEDA ISALYYYSTC GTQIPTLARS FMVCIQMISE
210 220 230 240 250
AARFQYIEGE MRTRIRYNRR SAPDPSVITL ENSWGRLSTA IQESNQGAFA
260 270 280 290 300
SPIQLQRRNG SKFNVYDVSI LIPIIALMVY RCAPPPSSQF SLLIRPVVPN
310 320 330 340 350
FNADVCMDPE PIVRIVGRNG LCVDVTGEEF FDGNPIQLWP CKSNTDWNQL
360 370 380 390 400
WTLRKDSTIR SNGKCLTISK SSPRQQVVIY NCSTATVGAT RWQIWDNRTI
410 420 430 440 450
INPRSGLVLA ATSGNSGTKL TVQTNIYAVS QGWLPTNNTQ PFVTTIVGLY
460 470 480 490 500
GMCLQANSGK VWLEDCTSEK AEQQWALYAD GSIRPQQNRD NCLTTDANIK
510 520 530 540 550
GTVVKILSCG PASSGQRWMF KNDGTILNLY NGLVLDVRRS DPSLKQIIVH
560
PFHGNLNQIW LPLF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 331 | F → T AA sequence (Ref. 2) Curated | 1 | |
Sequence conflicti | 362 | N → D AA sequence (Ref. 2) Curated | 1 | |
Sequence conflicti | 374 | R → G AA sequence (Ref. 2) Curated | 1 | |
Sequence conflicti | 404 | R → T AA sequence (Ref. 2) Curated | 1 | |
Sequence conflicti | 552 | F → V AA sequence (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12089 mRNA Translation: AAA33869.1 S40368 mRNA Translation: AAB22584.1 |
PIRi | A24261, RLCSAG |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - Glycan Binding RCA |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12089 mRNA Translation: AAA33869.1 S40368 mRNA Translation: AAB22584.1 |
PIRi | A24261, RLCSAG |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1RZO | X-ray | 2.63 | A/C | 25-286 | [»] | |
B/D | 303-564 | [»] | ||||
SMRi | P06750 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 |
UniLectini | P06750 |
PTM databases
GlyConnecti | 10, 3 N-Linked glycans |
Phylogenomic databases
eggNOGi | ENOG502QUVN, Eukaryota |
Miscellaneous databases
EvolutionaryTracei | P06750 |
Family and domain databases
CDDi | cd00161, RICIN, 2 hits |
Gene3Di | 3.40.420.10, 1 hit 4.10.470.10, 1 hit |
InterProi | View protein in InterPro IPR036041, Ribosome-inact_prot_sf IPR017989, Ribosome_inactivat_1/2 IPR001574, Ribosome_inactivat_prot IPR017988, Ribosome_inactivat_prot_CS IPR016138, Ribosome_inactivat_prot_sub1 IPR016139, Ribosome_inactivat_prot_sub2 IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
PANTHERi | PTHR33453, PTHR33453, 1 hit |
Pfami | View protein in Pfam PF00652, Ricin_B_lectin, 2 hits PF00161, RIP, 1 hit |
PRINTSi | PR00396, SHIGARICIN |
SMARTi | View protein in SMART SM00458, RICIN, 2 hits |
SUPFAMi | SSF50370, SSF50370, 2 hits SSF56371, SSF56371, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 2 hits PS00275, SHIGA_RICIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AGGL_RICCO | |
Accessioni | P06750Primary (citable) accession number: P06750 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families