UniProtKB - P06750 (AGGL_RICCO)

BLAST

>sp|P06750|AGGL_RICCO Agglutinin OS=Ricinus communis OX=3988 PE=1 SV=1
MYAVATWLCFGSTSGWSFTLEDNNIFPKQYPIINFTTADATVESYTNFIRAVRSHLTTGA
DVRHEIPVLPNRVGLPISQRFILVELSNHAELSVTLALDVTNAYVVGCRAGNSAYFFHPD
NQEDAEAITHLFTDVQNSFTFAFGGNYDRLEQLGGLRENIELGTGPLEDAISALYYYSTC
GTQIPTLARSFMVCIQMISEAARFQYIEGEMRTRIRYNRRSAPDPSVITLENSWGRLSTA
IQESNQGAFASPIQLQRRNGSKFNVYDVSILIPIIALMVYRCAPPPSSQFSLLIRPVVPN
FNADVCMDPEPIVRIVGRNGLCVDVTGEEFFDGNPIQLWPCKSNTDWNQLWTLRKDSTIR
SNGKCLTISKSSPRQQVVIYNCSTATVGATRWQIWDNRTIINPRSGLVLAATSGNSGTKL
TVQTNIYAVSQGWLPTNNTQPFVTTIVGLYGMCLQANSGKVWLEDCTSEKAEQQWALYAD
GSIRPQQNRDNCLTTDANIKGTVVKILSCGPASSGQRWMFKNDGTILNLYNGLVLDVRRS
DPSLKQIIVHPFHGNLNQIWLPLF

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History
Entry version 125 (18 Jul 2018)
Sequence version 1 (01 Jan 1988)
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Protein

Agglutinin

Gene

N/A

Organism

Ricinus communis (Castor bean)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalytic activityⁱ

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	74	Carbohydrate; via amide nitrogenBy similarity	1
Active siteⁱ	200	By similarity	1
Binding siteⁱ	312	Carbohydrate; via carbonyl oxygen	1
Binding siteⁱ	337	Carbohydrate	1
Binding siteⁱ	342	Carbohydrate	1
Binding siteⁱ	348	Carbohydrate	1
Binding siteⁱ	381	Carbohydrate	1
Binding siteⁱ	397	Carbohydrate	1
Binding siteⁱ	437	Carbohydrate	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	104 – 105	AMPBy similarity		2
Nucleotide bindingⁱ	145 – 147	AMPBy similarity		3

GO - Molecular functionⁱ

carbohydrate binding Source: UniProtKB-KW
nucleotide binding Source: UniProtKB-KW
rRNA N-glycosylase activity Source: UniProtKB-EC
toxin activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

defense response Source: UniProtKB-KW
negative regulation of translation Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protein synthesis inhibitor, Toxin
Biological process	Plant defense
Ligand	Lectin, Nucleotide-binding

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	CBM13 Carbohydrate-Binding Module Family 13
UniLectinⁱ	P06750

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Agglutinin Alternative name(s): RCA Cleaved into the following 2 chains: Agglutinin A chain (EC:3.2.2.22) Alternative name(s): rRNA N-glycosidase Agglutinin B chain
Organismⁱ	Ricinus communis (Castor bean)
Taxonomic identifierⁱ	3988 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Malpighiales › Euphorbiaceae › Acalyphoideae › Acalypheae › Ricinus

Subcellular locationⁱ

GO - Cellular component

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – 24	Sequence analysisAdd BLAST	24
ChainⁱPRO_0000030726	25 – 290	Agglutinin A chainAdd BLAST	266
PropeptideⁱPRO_0000030727	291 – 302	Linker peptide2 PublicationsAdd BLAST	12
ChainⁱPRO_0000030728	303 – 564	Agglutinin B chain1 PublicationAdd BLAST	262

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	34	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	259	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	282 ↔ 306	Interchain (between A and B chains)PROSITE-ProRule annotation1 Publication
Disulfide bondⁱ	322 ↔ 341	PROSITE-ProRule annotation1 Publication
Disulfide bondⁱ	365 ↔ 382	PROSITE-ProRule annotation1 Publication
Glycosylationⁱ	397	N-linked (GlcNAc...) asparagine	1
Glycosylationⁱ	437	N-linked (GlcNAc...) asparagine	1
Disulfide bondⁱ	453 ↔ 466	PROSITE-ProRule annotation1 Publication
Disulfide bondⁱ	492 ↔ 509	PROSITE-ProRule annotation1 Publication

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

PTM databases

GlyConnectⁱ	10
UniCarbKBⁱ	P06750

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	32 – 36	Combined sources	5
Helixⁱ	42 – 56	Combined sources	15
Beta strandⁱ	66 – 68	Combined sources	3
Helixⁱ	77 – 79	Combined sources	3
Beta strandⁱ	80 – 87	Combined sources	8
Beta strandⁱ	93 – 99	Combined sources	7
Turnⁱ	100 – 102	Combined sources	3
Beta strandⁱ	105 – 109	Combined sources	5
Beta strandⁱ	111 – 116	Combined sources	6
Helixⁱ	122 – 127	Combined sources	6
Helixⁱ	128 – 130	Combined sources	3
Beta strandⁱ	137 – 143	Combined sources	7
Helixⁱ	147 – 154	Combined sources	8
Helixⁱ	157 – 159	Combined sources	3
Helixⁱ	164 – 176	Combined sources	13
Helixⁱ	177 – 179	Combined sources	3
Helixⁱ	184 – 203	Combined sources	20
Helixⁱ	205 – 217	Combined sources	13
Helixⁱ	225 – 242	Combined sources	18
Beta strandⁱ	245 – 256	Combined sources	12
Beta strandⁱ	262 – 268	Combined sources	7
Helixⁱ	269 – 271	Combined sources	3
Turnⁱ	272 – 274	Combined sources	3
Helixⁱ	318 – 320	Combined sources	3
Beta strandⁱ	321 – 325	Combined sources	5
Helixⁱ	326 – 328	Combined sources	3
Beta strandⁱ	335 – 340	Combined sources	6
Helixⁱ	347 – 349	Combined sources	3
Beta strandⁱ	364 – 369	Combined sources	6
Beta strandⁱ	376 – 381	Combined sources	6
Turnⁱ	382 – 384	Combined sources	3
Helixⁱ	387 – 389	Combined sources	3
Beta strandⁱ	398 – 402	Combined sources	5
Turnⁱ	403 – 406	Combined sources	4
Beta strandⁱ	407 – 410	Combined sources	4
Beta strandⁱ	421 – 424	Combined sources	4
Helixⁱ	429 – 431	Combined sources	3
Beta strandⁱ	442 – 447	Combined sources	6
Helixⁱ	449 – 451	Combined sources	3
Beta strandⁱ	452 – 457	Combined sources	6
Beta strandⁱ	460 – 465	Combined sources	6
Helixⁱ	471 – 473	Combined sources	3
Beta strandⁱ	475 – 477	Combined sources	3
Beta strandⁱ	481 – 485	Combined sources	5
Beta strandⁱ	491 – 494	Combined sources	4
Beta strandⁱ	505 – 509	Combined sources	5
Turnⁱ	529 – 531	Combined sources	3
Beta strandⁱ	534 – 537	Combined sources	4
Helixⁱ	538 – 540	Combined sources	3
Beta strandⁱ	547 – 550	Combined sources	4
Helixⁱ	556 – 558	Combined sources	3
Beta strandⁱ	561 – 563	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	P06750
SMRⁱ	P06750
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P06750

EvolutionaryTraceⁱ

P06750

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	309 – 436	Ricin B-type lectin 1PROSITE-ProRule annotationAdd BLAST	128
Repeatⁱ	319 – 361	1-alphaAdd BLAST	43
Repeatⁱ	362 – 402	1-betaAdd BLAST	41
Repeatⁱ	405 – 437	1-gammaAdd BLAST	33
Domainⁱ	439 – 563	Ricin B-type lectin 2PROSITE-ProRule annotationAdd BLAST	125
Repeatⁱ	450 – 485	2-alphaAdd BLAST	36
Repeatⁱ	489 – 528	2-betaAdd BLAST	40
Repeatⁱ	531 – 558	2-gammaAdd BLAST	28

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	32 – 34	Carbohydrate binding	3
Regionⁱ	164 – 169	Carbohydrate bindingBy similarity	6
Regionⁱ	218 – 220	Carbohydrate bindingBy similarity	3
Regionⁱ	324 – 328	Carbohydrate binding	5
Regionⁱ	536 – 539	Carbohydrate bindingBy similarity	4
Regionⁱ	553 – 557	Carbohydrate bindingBy similarity	5

Sequence similaritiesⁱ

In the N-terminal section; belongs to the ribosome-inactivating protein family. Type 2 RIP subfamily.Curated

Keywords - Domainⁱ

Repeat, Signal

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00161 RICIN, 2 hits
Gene3Dⁱ	3.40.420.10, 1 hit 4.10.470.10, 1 hit
InterProⁱ	View protein in InterPro IPR036041 Ribosome-inact_prot_sf IPR017989 Ribosome_inactivat_1/2 IPR001574 Ribosome_inactivat_prot IPR017988 Ribosome_inactivat_prot_CS IPR016138 Ribosome_inactivat_prot_sub1 IPR016139 Ribosome_inactivat_prot_sub2 IPR035992 Ricin_B-like_lectins IPR000772 Ricin_B_lectin
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00652 Ricin_B_lectin, 2 hits PF00161 RIP, 1 hit
PRINTSⁱ	PR00396 SHIGARICIN
SMARTⁱ	View protein in SMART SM00458 RICIN, 2 hits
SUPFAMⁱ	SSF50370 SSF50370, 2 hits SSF56371 SSF56371, 1 hit
PROSITEⁱ	View protein in PROSITE PS50231 RICIN_B_LECTIN, 2 hits PS00275 SHIGA_RICIN, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P06750-1 [UniParc]FASTA Add to basket

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        10         20         30         40         50
MYAVATWLCF GSTSGWSFTL EDNNIFPKQY PIINFTTADA TVESYTNFIR 
        60         70         80         90        100
AVRSHLTTGA DVRHEIPVLP NRVGLPISQR FILVELSNHA ELSVTLALDV 
       110        120        130        140        150
TNAYVVGCRA GNSAYFFHPD NQEDAEAITH LFTDVQNSFT FAFGGNYDRL 
       160        170        180        190        200
EQLGGLRENI ELGTGPLEDA ISALYYYSTC GTQIPTLARS FMVCIQMISE 
       210        220        230        240        250
AARFQYIEGE MRTRIRYNRR SAPDPSVITL ENSWGRLSTA IQESNQGAFA 
       260        270        280        290        300
SPIQLQRRNG SKFNVYDVSI LIPIIALMVY RCAPPPSSQF SLLIRPVVPN 
       310        320        330        340        350
FNADVCMDPE PIVRIVGRNG LCVDVTGEEF FDGNPIQLWP CKSNTDWNQL 
       360        370        380        390        400
WTLRKDSTIR SNGKCLTISK SSPRQQVVIY NCSTATVGAT RWQIWDNRTI 
       410        420        430        440        450
INPRSGLVLA ATSGNSGTKL TVQTNIYAVS QGWLPTNNTQ PFVTTIVGLY 
       460        470        480        490        500
GMCLQANSGK VWLEDCTSEK AEQQWALYAD GSIRPQQNRD NCLTTDANIK 
       510        520        530        540        550
GTVVKILSCG PASSGQRWMF KNDGTILNLY NGLVLDVRRS DPSLKQIIVH 
       560 
PFHGNLNQIW LPLF

Length:564

Mass (Da):62,851

Last modified:January 1, 1988 - v1

Checksum:ⁱD455F2A72F609759

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	331	F → T AA sequence (Ref. 2) Curated	1
Sequence conflictⁱ	362	N → D AA sequence (Ref. 2) Curated	1
Sequence conflictⁱ	374	R → G AA sequence (Ref. 2) Curated	1
Sequence conflictⁱ	404	R → T AA sequence (Ref. 2) Curated	1
Sequence conflictⁱ	552	F → V AA sequence (Ref. 2) Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M12089 mRNA Translation: AAA33869.1 S40368 mRNA Translation: AAB22584.1
PIRⁱ	A24261 RLCSAG

Protein	Similar proteins		Species	Score	Length	Source
P06750	rRNA N-glycosidase		RICCO		575	UniRef90_P06750
UPI000049E3CA		RICCO		262
UPI000049E3C9		RICCO		262
UPI00077227F0		RICCO		605

Protein	Similar proteins		Species	Score	Length	Source
P06750	Ricin	)	RICCO		576	UniRef50_P02879
Abrin-a	)	ABRPR		528
Beta-galactoside-specific lectin 3		VISAL		569
rRNA N-glycosidase		CINCA		585
rRNA N-glycosidase		CAMSI		570
+101

Cross-referencesⁱ

Web resourcesⁱ

Functional Glycomics Gateway - Glycan Binding

RCA

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M12089 mRNA Translation: AAA33869.1 S40368 mRNA Translation: AAB22584.1
PIRⁱ	A24261 RLCSAG

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1RZO	X-ray	2.63	A/C	25-286	[»]
				B/D	303-564	[»]
ProteinModelPortalⁱ	P06750
SMRⁱ	P06750
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein family/group databases

CAZyⁱ	CBM13 Carbohydrate-Binding Module Family 13
UniLectinⁱ	P06750

PTM databases

GlyConnectⁱ	10
UniCarbKBⁱ	P06750

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P06750

EvolutionaryTraceⁱ

P06750

Family and domain databases

CDDⁱ	cd00161 RICIN, 2 hits
Gene3Dⁱ	3.40.420.10, 1 hit 4.10.470.10, 1 hit
InterProⁱ	View protein in InterPro IPR036041 Ribosome-inact_prot_sf IPR017989 Ribosome_inactivat_1/2 IPR001574 Ribosome_inactivat_prot IPR017988 Ribosome_inactivat_prot_CS IPR016138 Ribosome_inactivat_prot_sub1 IPR016139 Ribosome_inactivat_prot_sub2 IPR035992 Ricin_B-like_lectins IPR000772 Ricin_B_lectin
Pfamⁱ	View protein in Pfam PF00652 Ricin_B_lectin, 2 hits PF00161 RIP, 1 hit
PRINTSⁱ	PR00396 SHIGARICIN
SMARTⁱ	View protein in SMART SM00458 RICIN, 2 hits
SUPFAMⁱ	SSF50370 SSF50370, 2 hits SSF56371 SSF56371, 1 hit
PROSITEⁱ	View protein in PROSITE PS50231 RICIN_B_LECTIN, 2 hits PS00275 SHIGA_RICIN, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	AGGL_RICCO
Accessionⁱ	P06750Primary (citable) accession number: P06750
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
	Last sequence update:	January 1, 1988
	Last modified:	July 18, 2018
	This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

UniProtKB

UniParc

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Proteomes

Annotation systems

Supporting data

UniProtKB - P06750 (AGGL_RICCO)

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Agglutinin

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

PTM databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Family and domain databases

Experimental Info

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein family/group databases

PTM databases

Protocols and materials databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ