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Protein

Glucose-6-phosphate isomerase

Gene

Gpi

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Gpi), Glucose-6-phosphate isomerase (Gpi1), Glucose-6-phosphate isomerase (Gpi1), Glucose-6-phosphate isomerase (Gpi1)
  3. ATP-dependent 6-phosphofructokinase, muscle type (Pfkm), ATP-dependent 6-phosphofructokinase, liver type (Pfkl), ATP-dependent 6-phosphofructokinase, platelet type (Pfkp), ATP-dependent 6-phosphofructokinase (Pfkm), ATP-dependent 6-phosphofructokinase (Pfkp)
  4. Fructose-bisphosphate aldolase C (Aldoc), Fructose-bisphosphate aldolase B (Aldob), Fructose-bisphosphate aldolase A (Aldoa), Fructose-bisphosphate aldolase (Aldob), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoart1), Fructose-bisphosphate aldolase (Aldoart2), Fructose-bisphosphate aldolase (Aldob), Fructose-bisphosphate aldolase (Aldoart1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei358Proton donor1 Publication1
Active sitei3891 Publication1
Active sitei5191 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine, Growth factor, Isomerase
Biological processGluconeogenesis, Glycolysis

Enzyme and pathway databases

BRENDAi5.3.1.9 3474
ReactomeiR-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-6798695 Neutrophil degranulation
R-MMU-70171 Glycolysis
R-MMU-70263 Gluconeogenesis
SABIO-RKiP06745
UniPathwayi
UPA00109;UER00181

Protein family/group databases

MoonProtiP06745

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Autocrine motility factor
Short name:
AMF
Neuroleukin
Short name:
NLK
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:Gpi
Synonyms:Gpi1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:95797 Gpi1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001805382 – 558Glucose-6-phosphate isomeraseAdd BLAST557

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei12N6-acetyllysineBy similarity1
Modified residuei86PhosphoserineBy similarity1
Modified residuei107PhosphoserineBy similarity1
Modified residuei109PhosphothreonineBy similarity1
Modified residuei142N6-acetyllysineCombined sources1
Modified residuei185Phosphoserine; by CK2By similarity1
Modified residuei250PhosphothreonineBy similarity1
Modified residuei454N6-acetyllysine; alternateCombined sources1
Modified residuei454N6-malonyllysine; alternateBy similarity1
Modified residuei454N6-succinyllysine; alternateCombined sources1
Modified residuei455PhosphoserineCombined sources1

Post-translational modificationi

ISGylated.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP06745
MaxQBiP06745
PaxDbiP06745
PeptideAtlasiP06745
PRIDEiP06745

PTM databases

iPTMnetiP06745
PhosphoSitePlusiP06745
SwissPalmiP06745

Expressioni

Gene expression databases

BgeeiENSMUSG00000036427 Expressed in 338 organ(s), highest expression level in CA1 field of hippocampus
CleanExiMM_GPI1
ExpressionAtlasiP06745 baseline and differential
GenevisibleiP06745 MM

Interactioni

Subunit structurei

Homodimer in the catalytically active form, monomer in the secreted form.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Parp14Q2EMV94EBI-1534927,EBI-1534943

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200021, 5 interactors
IntActiP06745, 7 interactors
MINTiP06745
STRINGi10090.ENSMUSP00000049355

Structurei

Secondary structure

1558
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP06745
SMRiP06745
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06745

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Phylogenomic databases

eggNOGiKOG2446 Eukaryota
COG0166 LUCA
GeneTreeiENSGT00390000000707
HOVERGENiHBG002877
InParanoidiP06745
KOiK01810
OMAiTNSQHAF
OrthoDBiEOG091G03ZI
PhylomeDBiP06745
TreeFamiTF300436

Family and domain databases

CDDicd05015 SIS_PGI_1, 1 hit
cd05016 SIS_PGI_2, 1 hit
Gene3Di1.10.1390.10, 1 hit
HAMAPiMF_00473 G6P_isomerase, 1 hit
InterProiView protein in InterPro
IPR001672 G6P_Isomerase
IPR023096 G6P_Isomerase_C
IPR018189 Phosphoglucose_isomerase_CS
IPR035476 SIS_PGI_1
IPR035482 SIS_PGI_2
PANTHERiPTHR11469 PTHR11469, 1 hit
PfamiView protein in Pfam
PF00342 PGI, 1 hit
PRINTSiPR00662 G6PISOMERASE
PROSITEiView protein in PROSITE
PS00765 P_GLUCOSE_ISOMERASE_1, 1 hit
PS00174 P_GLUCOSE_ISOMERASE_2, 1 hit
PS51463 P_GLUCOSE_ISOMERASE_3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

P06745-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAALTRNPQF QKLLEWHRAN SANLKLRELF EADPERFNNF SLNLNTNHGH
60 70 80 90 100
ILVDYSKNLV NKEVMQMLVE LAKSRGVEAA RDNMFSGSKI NYTENRAVLH
110 120 130 140 150
VALRNRSNTP IKVDGKDVMP EVNRVLDKMK SFCQRVRSGD WKGYTGKSIT
160 170 180 190 200
DIINIGIGGS DLGPLMVTEA LKPYSKGGPR VWFVSNIDGT HIAKTLASLS
210 220 230 240 250
PETSLFIIAS KTFTTQETIT NAETAKEWFL EAAKDPSAVA KHFVALSTNT
260 270 280 290 300
AKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DHFEQLLSGA
310 320 330 340 350
HWMDQHFLKT PLEKNAPVLL ALLGIWYINC YGCETHALLP YDQYMHRFAA
360 370 380 390 400
YFQQGDMESN GKYITKSGAR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK
410 420 430 440 450
MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMKGK LPEEARKELQ
460 470 480 490 500
AAGKSPEDLE KLLPHKVFEG NRPTNSIVFT KLTPFILGAL IAMYEHKIFV
510 520 530 540 550
QGIMWDINSF DQWGVELGKQ LAKKIEPELE GSSAVTSHDS STNGLISFIK

QQRDTKLE
Length:558
Mass (Da):62,767
Last modified:May 1, 2007 - v4
Checksum:i7299E98B12B4C375
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RQ18A0A0U1RQ18_MOUSE
Glucose-6-phosphate isomerase
Gpi1
191Annotation score:
A0A0U1RP97A0A0U1RP97_MOUSE
Glucose phosphate isomerase 1
Gpi1
152Annotation score:
A0A0U1RPC9A0A0U1RPC9_MOUSE
Glucose phosphate isomerase 1
Gpi1
83Annotation score:
A0A0U1RQ72A0A0U1RQ72_MOUSE
Glucose phosphate isomerase 1
Gpi1
130Annotation score:
A0A0U1RP58A0A0U1RP58_MOUSE
Glucose phosphate isomerase 1
Gpi1
34Annotation score:

Sequence cautioni

The sequence BAC36335 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95N → D in AAA39825 (PubMed:3764429).Curated1
Sequence conflicti95N → D in BAE41301 (PubMed:3764429).Curated1
Sequence conflicti119M → K in BAE21866 (PubMed:16141072).Curated1
Sequence conflicti191H → R in BAE22329 (PubMed:16141072).Curated1
Sequence conflicti238A → E in AAC36515 (PubMed:9798653).Curated1
Sequence conflicti264 – 266FEF → LEL in AAC36515 (PubMed:9798653).Curated3
Sequence conflicti285A → V in AAC36515 (PubMed:9798653).Curated1
Sequence conflicti303M → T in AAC36515 (PubMed:9798653).Curated1
Sequence conflicti313E → G in BAE35416 (PubMed:16141072).Curated1
Sequence conflicti357M → V in AAC36515 (PubMed:9798653).Curated1
Sequence conflicti372D → N in AAC36515 (PubMed:9798653).Curated1
Sequence conflicti380W → L in AAC36515 (PubMed:9798653).Curated1
Sequence conflicti499F → L in BAE23502 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14220 mRNA Translation: AAA39825.1
AK076424 mRNA Translation: BAC36335.1 Different initiation.
AK133827 mRNA Translation: BAE21866.1
AK134890 mRNA Translation: BAE22329.1
AK137805 mRNA Translation: BAE23502.1
AK147124 mRNA Translation: BAE27695.1
AK150341 mRNA Translation: BAE29481.1
AK159838 mRNA Translation: BAE35416.1
AK169681 mRNA Translation: BAE41301.1
BX537302 Genomic DNA No translation available.
BC086640 mRNA Translation: AAH86640.1
BC088995 mRNA Translation: AAH88995.1
U89408 mRNA Translation: AAC36515.1
L09104 mRNA Translation: AAA65641.1
AF108354 mRNA Translation: AAF28799.1
CCDSiCCDS21138.1
PIRiA24439 NUMS
RefSeqiNP_032181.2, NM_008155.4
UniGeneiMm.589

Genome annotation databases

EnsembliENSMUST00000038027; ENSMUSP00000049355; ENSMUSG00000036427
GeneIDi14751
KEGGimmu:14751
UCSCiuc009gix.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14220 mRNA Translation: AAA39825.1
AK076424 mRNA Translation: BAC36335.1 Different initiation.
AK133827 mRNA Translation: BAE21866.1
AK134890 mRNA Translation: BAE22329.1
AK137805 mRNA Translation: BAE23502.1
AK147124 mRNA Translation: BAE27695.1
AK150341 mRNA Translation: BAE29481.1
AK159838 mRNA Translation: BAE35416.1
AK169681 mRNA Translation: BAE41301.1
BX537302 Genomic DNA No translation available.
BC086640 mRNA Translation: AAH86640.1
BC088995 mRNA Translation: AAH88995.1
U89408 mRNA Translation: AAC36515.1
L09104 mRNA Translation: AAA65641.1
AF108354 mRNA Translation: AAF28799.1
CCDSiCCDS21138.1
PIRiA24439 NUMS
RefSeqiNP_032181.2, NM_008155.4
UniGeneiMm.589

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U0EX-ray1.60A/B1-558[»]
1U0FX-ray1.60A/B1-558[»]
1U0GX-ray1.70A/B1-558[»]
2CVPX-ray1.80A/B1-557[»]
2CXNX-ray1.40A/B1-557[»]
2CXOX-ray1.80A/B1-557[»]
2CXPX-ray1.70A/B1-557[»]
2CXQX-ray1.50A/B1-557[»]
2CXRX-ray1.70A/B1-557[»]
2CXSX-ray1.50A/B1-557[»]
2CXTX-ray1.50A/B1-557[»]
2CXUX-ray1.65A/B1-557[»]
ProteinModelPortaliP06745
SMRiP06745
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200021, 5 interactors
IntActiP06745, 7 interactors
MINTiP06745
STRINGi10090.ENSMUSP00000049355

Protein family/group databases

MoonProtiP06745

PTM databases

iPTMnetiP06745
PhosphoSitePlusiP06745
SwissPalmiP06745

Proteomic databases

EPDiP06745
MaxQBiP06745
PaxDbiP06745
PeptideAtlasiP06745
PRIDEiP06745

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038027; ENSMUSP00000049355; ENSMUSG00000036427
GeneIDi14751
KEGGimmu:14751
UCSCiuc009gix.3 mouse

Organism-specific databases

CTDi14751
MGIiMGI:95797 Gpi1

Phylogenomic databases

eggNOGiKOG2446 Eukaryota
COG0166 LUCA
GeneTreeiENSGT00390000000707
HOVERGENiHBG002877
InParanoidiP06745
KOiK01810
OMAiTNSQHAF
OrthoDBiEOG091G03ZI
PhylomeDBiP06745
TreeFamiTF300436

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00181

BRENDAi5.3.1.9 3474
ReactomeiR-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-6798695 Neutrophil degranulation
R-MMU-70171 Glycolysis
R-MMU-70263 Gluconeogenesis
SABIO-RKiP06745

Miscellaneous databases

EvolutionaryTraceiP06745
PROiPR:P06745
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036427 Expressed in 338 organ(s), highest expression level in CA1 field of hippocampus
CleanExiMM_GPI1
ExpressionAtlasiP06745 baseline and differential
GenevisibleiP06745 MM

Family and domain databases

CDDicd05015 SIS_PGI_1, 1 hit
cd05016 SIS_PGI_2, 1 hit
Gene3Di1.10.1390.10, 1 hit
HAMAPiMF_00473 G6P_isomerase, 1 hit
InterProiView protein in InterPro
IPR001672 G6P_Isomerase
IPR023096 G6P_Isomerase_C
IPR018189 Phosphoglucose_isomerase_CS
IPR035476 SIS_PGI_1
IPR035482 SIS_PGI_2
PANTHERiPTHR11469 PTHR11469, 1 hit
PfamiView protein in Pfam
PF00342 PGI, 1 hit
PRINTSiPR00662 G6PISOMERASE
PROSITEiView protein in PROSITE
PS00765 P_GLUCOSE_ISOMERASE_1, 1 hit
PS00174 P_GLUCOSE_ISOMERASE_2, 1 hit
PS51463 P_GLUCOSE_ISOMERASE_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiG6PI_MOUSE
AccessioniPrimary (citable) accession number: P06745
Secondary accession number(s): O89062
, Q3TEE7, Q3TW50, Q3UUX1, Q3UY84, Q3UZJ1, Q5RJI3, Q8C675, Q9JM07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: May 1, 2007
Last modified: November 7, 2018
This is version 167 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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