UniProtKB - P06738 (PHSG_YEAST)
Protein
Glycogen phosphorylase
Gene
GPH1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Catalytic activityi
- EC:2.4.1.1
Cofactori
Activity regulationi
Activated by phosphorylation of Thr-31.
GO - Molecular functioni
- glycogen phosphorylase activity Source: SGD
- linear malto-oligosaccharide phosphorylase activity Source: UniProtKB-EC
- pyridoxal phosphate binding Source: GO_Central
- SHG alpha-glucan phosphorylase activity Source: UniProtKB-EC
GO - Biological processi
- glycogen catabolic process Source: SGD
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Carbohydrate metabolism, Glycogen metabolism |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | MetaCyc:YPR160W-MONOMER |
Reactomei | R-SCE-6798695, Neutrophil degranulation R-SCE-70221, Glycogen breakdown (glycogenolysis) |
Protein family/group databases
CAZyi | GT35, Glycosyltransferase Family 35 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:GPH1 Ordered Locus Names:YPR160W ORF Names:P9584.1 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006364, GPH1 |
VEuPathDBi | FungiDB:YPR160W |
Subcellular locationi
Other locations
- cytoplasm Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000188545 | 2 – 902 | Glycogen phosphorylaseAdd BLAST | 901 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 31 | PhosphothreonineCombined sources2 Publications | 1 | |
Modified residuei | 333 | PhosphoserineCombined sources | 1 | |
Modified residuei | 751 | N6-(pyridoxal phosphate)lysine | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P06738 |
PaxDbi | P06738 |
PRIDEi | P06738 |
PTM databases
iPTMneti | P06738 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 36333, 285 interactors |
DIPi | DIP-2648N |
IntActi | P06738, 26 interactors |
MINTi | P06738 |
STRINGi | 4932.YPR160W |
Miscellaneous databases
RNActi | P06738, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P06738 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06738 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycogen phosphorylase family.Curated
Phylogenomic databases
eggNOGi | KOG2099, Eukaryota |
GeneTreei | ENSGT00950000183148 |
HOGENOMi | CLU_010198_1_0_1 |
InParanoidi | P06738 |
OMAi | KSPIDFN |
Family and domain databases
InterProi | View protein in InterPro IPR011833, Glycg_phsphrylas IPR000811, Glyco_trans_35 IPR035090, Pyridoxal_P_attach_site |
PANTHERi | PTHR11468, PTHR11468, 1 hit |
Pfami | View protein in Pfam PF00343, Phosphorylase, 1 hit |
PIRSFi | PIRSF000460, Pprylas_GlgP, 1 hit |
TIGRFAMsi | TIGR02093, P_ylase, 1 hit |
PROSITEi | View protein in PROSITE PS00102, PHOSPHORYLASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P06738-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPPASTSTTN DMITEEPTSP HQIPRLTRRL TGFLPQEIKS IDTMIPLKSR
60 70 80 90 100
ALWNKHQVKK FNKAEDFQDR FIDHVETTLA RSLYNCDDMA AYEAASMSIR
110 120 130 140 150
DNLVIDWNKT QQKFTTRDPK RVYYLSLEFL MGRALDNALI NMKIEDPEDP
160 170 180 190 200
AASKGKPREM IKGALDDLGF KLEDVLDQEP DAGLGNGGLG RLAACFVDSM
210 220 230 240 250
ATEGIPAWGY GLRYEYGIFA QKIIDGYQVE TPDYWLNSGN PWEIERNEVQ
260 270 280 290 300
IPVTFYGYVD RPEGGKTTLS ASQWIGGERV LAVAYDFPVP GFKTSNVNNL
310 320 330 340 350
RLWQARPTTE FDFAKFNNGD YKNSVAQQQR AESITAVLYP NDNFAQGKEL
360 370 380 390 400
RLKQQYFWCA ASLHDILRRF KKSKRPWTEF PDQVAIQLND THPTLAIVEL
410 420 430 440 450
QRVLVDLEKL DWHEAWDIVT KTFAYTNHTV MQEALEKWPV GLFGHLLPRH
460 470 480 490 500
LEIIYDINWF FLQDVAKKFP KDVDLLSRIS IIEENSPERQ IRMAFLAIVG
510 520 530 540 550
SHKVNGVAEL HSELIKTTIF KDFVKFYGPS KFVNVTNGIT PRRWLKQANP
560 570 580 590 600
SLAKLISETL NDPTEEYLLD MAKLTQLGKY VEDKEFLKKW NQVKLNNKIR
610 620 630 640 650
LVDLIKKEND GVDIINREYL DDTLFDMQVK RIHEYKRQQL NVFGIIYRYL
660 670 680 690 700
AMKNMLKNGA SIEEVAKKYP RKVSIFGGKS APGYYMAKLI IKLINCVADI
710 720 730 740 750
VNNDESIEHL LKVVFVADYN VSKAEIIIPA SDLSEHISTA GTEASGTSNM
760 770 780 790 800
KFVMNGGLII GTVDGANVEI TREIGEDNVF LFGNLSENVE ELRYNHQYHP
810 820 830 840 850
QDLPSSLDSV LSYIESGQFS PENPNEFKPL VDSIKYHGDY YLVSDDFESY
860 870 880 890 900
LATHELVDQE FHNQRSEWLK KSVLSVANVG FFSSDRCIEE YSDTIWNVEP
VT
Sequence cautioni
The sequence CAA28273 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 167 – 168 | DL → EG in CAA28273 (PubMed:3537803).Curated | 2 | |
Sequence conflicti | 167 – 168 | DL → EG in AAB59313 (PubMed:7871892).Curated | 2 | |
Sequence conflicti | 326 | A → P in CAA28273 (PubMed:3537803).Curated | 1 | |
Sequence conflicti | 326 | A → P in AAB59313 (PubMed:7871892).Curated | 1 | |
Sequence conflicti | 440 – 441 | VG → RR in CAA28273 (PubMed:3537803).Curated | 2 | |
Sequence conflicti | 440 – 441 | VG → RR in AAB59313 (PubMed:7871892).Curated | 2 | |
Sequence conflicti | 508 | A → V in CAA28273 (PubMed:3537803).Curated | 1 | |
Sequence conflicti | 508 | A → V in AAB59313 (PubMed:7871892).Curated | 1 | |
Sequence conflicti | 524 | V → I in CAA28273 (PubMed:3537803).Curated | 1 | |
Sequence conflicti | 524 | V → I in AAB59313 (PubMed:7871892).Curated | 1 | |
Sequence conflicti | 578 | G → E in CAA28273 (PubMed:3537803).Curated | 1 | |
Sequence conflicti | 578 | G → E in AAB59313 (PubMed:7871892).Curated | 1 | |
Sequence conflicti | 876 | V → L in CAA28273 (PubMed:3537803).Curated | 1 | |
Sequence conflicti | 876 | V → L in AAB59313 (PubMed:7871892).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04604 Genomic DNA Translation: CAA28273.1 Different initiation. L33835 Genomic DNA Translation: AAB59313.1 U28371 Genomic DNA Translation: AAB68057.1 BK006949 Genomic DNA Translation: DAA11577.1 |
PIRi | S61144 |
RefSeqi | NP_015486.1, NM_001184257.1 |
Genome annotation databases
EnsemblFungii | YPR160W_mRNA; YPR160W; YPR160W |
GeneIDi | 856289 |
KEGGi | sce:YPR160W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04604 Genomic DNA Translation: CAA28273.1 Different initiation. L33835 Genomic DNA Translation: AAB59313.1 U28371 Genomic DNA Translation: AAB68057.1 BK006949 Genomic DNA Translation: DAA11577.1 |
PIRi | S61144 |
RefSeqi | NP_015486.1, NM_001184257.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YGP | X-ray | 2.80 | A/B | 24-902 | [»] | |
SMRi | P06738 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36333, 285 interactors |
DIPi | DIP-2648N |
IntActi | P06738, 26 interactors |
MINTi | P06738 |
STRINGi | 4932.YPR160W |
Protein family/group databases
CAZyi | GT35, Glycosyltransferase Family 35 |
PTM databases
iPTMneti | P06738 |
Proteomic databases
MaxQBi | P06738 |
PaxDbi | P06738 |
PRIDEi | P06738 |
Genome annotation databases
EnsemblFungii | YPR160W_mRNA; YPR160W; YPR160W |
GeneIDi | 856289 |
KEGGi | sce:YPR160W |
Organism-specific databases
SGDi | S000006364, GPH1 |
VEuPathDBi | FungiDB:YPR160W |
Phylogenomic databases
eggNOGi | KOG2099, Eukaryota |
GeneTreei | ENSGT00950000183148 |
HOGENOMi | CLU_010198_1_0_1 |
InParanoidi | P06738 |
OMAi | KSPIDFN |
Enzyme and pathway databases
BioCyci | MetaCyc:YPR160W-MONOMER |
Reactomei | R-SCE-6798695, Neutrophil degranulation R-SCE-70221, Glycogen breakdown (glycogenolysis) |
Miscellaneous databases
EvolutionaryTracei | P06738 |
PROi | PR:P06738 |
RNActi | P06738, protein |
Family and domain databases
InterProi | View protein in InterPro IPR011833, Glycg_phsphrylas IPR000811, Glyco_trans_35 IPR035090, Pyridoxal_P_attach_site |
PANTHERi | PTHR11468, PTHR11468, 1 hit |
Pfami | View protein in Pfam PF00343, Phosphorylase, 1 hit |
PIRSFi | PIRSF000460, Pprylas_GlgP, 1 hit |
TIGRFAMsi | TIGR02093, P_ylase, 1 hit |
PROSITEi | View protein in PROSITE PS00102, PHOSPHORYLASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHSG_YEAST | |
Accessioni | P06738Primary (citable) accession number: P06738 Secondary accession number(s): D6W4G1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | October 5, 2010 | |
Last modified: | February 10, 2021 | |
This is version 198 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families