ENO1

Functionⁱ

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

Miscellaneous

Used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.

Catalytic activityⁱ

2-phospho-D-glycerate = phosphoenolpyruvate + H₂O.1 Publication

Cofactorⁱ

Mg²⁺1 PublicationNote: Binds two Mg²⁺ per subunit. Required for catalysis and for stabilizing the dimer.1 Publication

pH dependenceⁱ

Enolase activity is lost above pH 9.0. Immunoglobulin production stimulating activity is retained at pH 13.0.1 Publication

Pathwayⁱ: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:

This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	40	Magnesium 1	1
Binding siteⁱ	158	SubstrateBy similarity	1
Binding siteⁱ	167	SubstrateBy similarity	1
Active siteⁱ	210	Proton donorBy similarity	1
Metal bindingⁱ	245	Magnesium 2	1
Metal bindingⁱ	293	Magnesium 2	1
Binding siteⁱ	293	SubstrateBy similarity	1
Metal bindingⁱ	318	Magnesium 2	1
Binding siteⁱ	318	SubstrateBy similarity	1
Active siteⁱ	343	Proton acceptorBy similarity	1
Binding siteⁱ	394	SubstrateBy similarity	1

GO - Molecular functionⁱ

cadherin binding
Source: BHF-UCL
Inferred from high throughput direct assayⁱ
- 25468996
DNA-binding transcription repressor activity, RNA polymerase II-specific
Source: NTNU_SB
Inferred from direct assayⁱ
- 2005901
GTPase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 24337748
magnesium ion binding Source: InterPro
phosphopyruvate hydratase activity
Source: CAFA
Inferred from mutant phenotypeⁱ
- 3529090
protein homodimerization activity
Source: CAFA
Inferred from direct assayⁱ
- 3529090
proximal promoter DNA-binding transcription repressor activity, RNA polymerase II-specific
Source: CAFA
Inferred from direct assayⁱ
- 2005901
RNA binding
Source: UniProtKB
Inferred from high throughput direct assayⁱ
- 22658674
RNA polymerase II regulatory region sequence-specific DNA binding
Source: NTNU_SB
Inferred from direct assayⁱ
- 2005901

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

canonical glycolysis
Source: CAFA
Inferred from mutant phenotypeⁱ
- 3529090
gluconeogenesis Source: Reactome
negative regulation of cell growth
Source: UniProtKB
Inferred from direct assayⁱ
- 10082554
negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
Source: CAFA
Inferred from direct assayⁱ
- 15459207
negative regulation of transcription, DNA-templated
Source: UniProtKB
Inferred from direct assayⁱ
- 10082554
negative regulation of transcription by RNA polymerase II
Source: CAFA
Inferred from direct assayⁱ
- 2005901
positive regulation of ATP biosynthetic process
Source: CAFA
Inferred from direct assayⁱ
- 15459207
positive regulation of muscle contraction
Source: CAFA
Inferred from genetic interactionⁱ
- 15459207
positive regulation of plasminogen activation
Source: CAFA
Inferred from mutant phenotypeⁱ
- 12666133
response to virus
Source: UniProtKB
Inferred from expression patternⁱ
- 16548883

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Lyase, Repressor
Biological process	Glycolysis, Plasminogen activation, Transcription, Transcription regulation
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

BioCycⁱ	MetaCyc:ENSG00000074800-MONOMER
BRENDAⁱ	4.2.1.11 2681
Reactomeⁱ	R-HSA-70171 Glycolysis R-HSA-70263 Gluconeogenesis
SABIO-RKⁱ	P06733
SIGNORⁱ	P06733
UniPathwayⁱ	UPA00109;UER00187

Protein family/group databases

MoonDBⁱ	P06733 Predicted
MoonProtⁱ	P06733

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Alpha-enolase (EC:4.2.1.11) Alternative name(s): 2-phospho-D-glycerate hydro-lyase C-myc promoter-binding protein Enolase 1 MBP-1 MPB-1 Non-neural enolase Short name: NNE Phosphopyruvate hydratase Plasminogen-binding protein
Gene namesⁱ	Name:ENO1 Synonyms:ENO1L1, MBPB1, MPB1
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000074800.13
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:3350 ENO1
MIMⁱ	172430 gene
neXtProtⁱ	NX_P06733

Keywords - Cellular componentⁱ

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	94	M → I: MBP1 protein production. No MBP1 protein production; when associated with I-97. 1 Publication	1
Mutagenesisⁱ	97	M → I: MBP1 protein production. No MBP1 protein production; when associated with I-94. 1 Publication	1
Mutagenesisⁱ	384	L → A: Loss of transcriptional repression and cell growth inhibition; when associated with A-388. 1 Publication	1
Mutagenesisⁱ	388	L → A: Loss of transcriptional repression and cell growth inhibition; when associated with A-384. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	2023
Open Targets More...OpenTargetsⁱ	ENSG00000074800
PharmGKBⁱ	PA27786

Chemistry databases

ChEMBLⁱ	CHEMBL3298

ChEMBLⁱ

CHEMBL3298

Polymorphism and mutation databases

BioMutaⁱ	ENO1
DMDMⁱ	119339

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources1 Publication
ChainⁱPRO_0000134097	2 – 434	Alpha-enolaseAdd BLAST		433

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylserineCombined sources1 Publication	1
Modified residueⁱ	5	N6-acetyllysineCombined sources	1
Modified residueⁱ	27	PhosphoserineCombined sources	1
Modified residueⁱ	44	PhosphotyrosineCombined sources	1
Modified residueⁱ	60	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	60	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	64	N6-acetyllysineCombined sources	1
Modified residueⁱ	71	N6-acetyllysineCombined sources	1
Modified residueⁱ	89	N6-acetyllysine; alternateCombined sources	1
Modified residueⁱ	89	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	92	N6-acetyllysineBy similarity	1
Modified residueⁱ	126	N6-acetyllysineCombined sources	1
Modified residueⁱ	193	N6-acetyllysineCombined sources	1
Modified residueⁱ	199	N6-acetyllysineCombined sources	1
Modified residueⁱ	202	N6-acetyllysine; alternateBy similarity	1
Cross-linkⁱ	202	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residueⁱ	228	N6-acetyllysine; alternateCombined sources	1
Modified residueⁱ	228	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	233	N6-acetyllysine; alternateCombined sources	1
Modified residueⁱ	233	N6-malonyllysine; alternate1 Publication	1
Modified residueⁱ	254	PhosphoserineCombined sources	1
Modified residueⁱ	256	N6-acetyllysineCombined sources	1
Modified residueⁱ	263	PhosphoserineCombined sources	1
Modified residueⁱ	272	PhosphoserineCombined sources	1
Modified residueⁱ	281	N6-acetyllysineCombined sources	1
Modified residueⁱ	285	N6-acetyllysineCombined sources	1
Modified residueⁱ	287	PhosphotyrosineCombined sources	1
Modified residueⁱ	291	PhosphoserineCombined sources	1
Modified residueⁱ	335	N6-acetyllysineBy similarity	1
Modified residueⁱ	343	N6-acetyllysineBy similarity	1
Modified residueⁱ	406	N6-acetyllysineBy similarity	1
Modified residueⁱ	420	N6-acetyllysine; alternateCombined sources	1
Modified residueⁱ	420	N6-malonyllysine; alternate1 Publication	1
Modified residueⁱ	420	N6-succinyllysine; alternateBy similarity	1

Post-translational modificationⁱ

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	P06733
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P06733
PaxDbⁱ	P06733
PeptideAtlas More...PeptideAtlasⁱ	P06733
PRIDEⁱ	P06733
ProteomicsDBⁱ	51919 51920 [P06733-2]
TopDownProteomicsⁱ	P06733-1 [P06733-1] P06733-2 [P06733-2]

2D gel databases

DOSAC-COBS 2D-PAGE database More...DOSAC-COBS-2DPAGEⁱ	P06733
USC-OGP 2-DE database More...OGPⁱ	P06733
REPRODUCTION-2DPAGE More...REPRODUCTION-2DPAGEⁱ	IPI00465248 P06733
SWISS-2DPAGEⁱ	P06733
UCD-2DPAGEⁱ	P06733

PTM databases

iPTMnetⁱ	P06733
PhosphoSitePlusⁱ	P06733
SwissPalmⁱ	P06733

Miscellaneous databases

PMAP-CutDBⁱ	P06733

PMAP-CutDBⁱ

P06733

Expressionⁱ

Tissue specificityⁱ

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Developmental stageⁱ

During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

Inductionⁱ

Induced in diffuse large cell lymphoma (DLCL) after treatment with the natural biological agent, Bryo1. Up-regulated in response to enterovirus 71 (EV71) infection (at protein level).2 Publications

Gene expression databases

Bgeeⁱ	ENSG00000074800 Expressed in 231 organ(s), highest expression level in cortex of kidney
ExpressionAtlasⁱ	P06733 baseline and differential
Genevisibleⁱ	P06733 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB018614 CAB069394 HPA068284 HPA068721

HPAⁱ

CAB018614
CAB069394
HPA068284
HPA068721

Interactionⁱ

Subunit structureⁱ

Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific (PubMed:18560153). ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding (PubMed:9308760). Isoform MBP-1 interacts with TRAPPC2B (PubMed:11134351). Interacts with ENO4 and PGAM2 (By similarity). Interacts with CMTM6 (PubMed:28813417).By similarity4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
ACHE	P22303	2	EBI-353877,EBI-1637793
Akt2	Q60823	2	EBI-353877,EBI-400263	From Mus musculus.
PCNA	P12004	3	EBI-353877,EBI-358311
TTN	Q8WZ42	3	EBI-353877,EBI-681210
YWHAZ	P63104	2	EBI-353877,EBI-347088

GO - Molecular functionⁱ

cadherin binding
Source: BHF-UCL
Inferred from high throughput direct assayⁱ
- 25468996
GTPase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 24337748
protein homodimerization activity
Source: CAFA
Inferred from direct assayⁱ
- 3529090

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	108338, 296 interactors
IntActⁱ	P06733, 90 interactors
Molecular INTeraction database More...MINTⁱ	P06733
STRINGⁱ	9606.ENSP00000234590

Chemistry databases

BindingDBⁱ

P06733

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	5 – 12	Combined sources	8
Beta strandⁱ	18 – 26	Combined sources	9
Beta strandⁱ	29 – 34	Combined sources	6
Helixⁱ	57 – 59	Combined sources	3
Helixⁱ	63 – 71	Combined sources	9
Helixⁱ	73 – 79	Combined sources	7
Helixⁱ	87 – 98	Combined sources	12
Turnⁱ	104 – 106	Combined sources	3
Helixⁱ	108 – 125	Combined sources	18
Helixⁱ	130 – 138	Combined sources	9
Beta strandⁱ	147 – 154	Combined sources	8
Helixⁱ	156 – 158	Combined sources	3
Beta strandⁱ	159 – 162	Combined sources	4
Beta strandⁱ	167 – 171	Combined sources	5
Helixⁱ	178 – 200	Combined sources	23
Helixⁱ	202 – 204	Combined sources	3
Helixⁱ	220 – 233	Combined sources	14
Turnⁱ	237 – 239	Combined sources	3
Beta strandⁱ	241 – 245	Combined sources	5
Helixⁱ	248 – 250	Combined sources	3
Turnⁱ	259 – 262	Combined sources	4
Helixⁱ	267 – 269	Combined sources	3
Helixⁱ	273 – 286	Combined sources	14
Beta strandⁱ	289 – 293	Combined sources	5
Helixⁱ	301 – 311	Combined sources	11
Beta strandⁱ	313 – 318	Combined sources	6
Turnⁱ	319 – 323	Combined sources	5
Helixⁱ	325 – 334	Combined sources	10
Beta strandⁱ	338 – 342	Combined sources	5
Helixⁱ	344 – 347	Combined sources	4
Helixⁱ	350 – 362	Combined sources	13
Beta strandⁱ	366 – 370	Combined sources	5
Helixⁱ	380 – 387	Combined sources	8
Beta strandⁱ	391 – 394	Combined sources	4
Helixⁱ	401 – 417	Combined sources	17
Helixⁱ	418 – 420	Combined sources	3
Helixⁱ	425 – 427	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	P06733
SMRⁱ	P06733
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P06733

EvolutionaryTraceⁱ

P06733

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	31 – 38	Epitope recognized by CAR and healthy patient antibodies	8
Regionⁱ	56 – 63	Epitope recognized by CAR antibodies	8
Regionⁱ	97 – 237	Required for repression of c-myc promoter activityAdd BLAST	141
Regionⁱ	370 – 373	Substrate bindingBy similarity	4
Regionⁱ	405 – 434	Required for interaction with PLGBy similarityAdd BLAST	30

Sequence similaritiesⁱ

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG2670 Eukaryota COG0148 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00910000144064
HOVERGENⁱ	HBG000067
InParanoidⁱ	P06733
KEGG Orthology (KO) More...KOⁱ	K01689
OMAⁱ	EFMIIPV
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G07NH
PhylomeDBⁱ	P06733
TreeFamⁱ	TF300391

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd03313 enolase, 1 hit
Gene3Dⁱ	3.20.20.120, 1 hit 3.30.390.10, 1 hit
HAMAPⁱ	MF_00318 Enolase, 1 hit
InterProⁱ	View protein in InterPro IPR000941 Enolase IPR036849 Enolase-like_C_sf IPR029017 Enolase-like_N IPR034390 Enolase-like_superfamily IPR020810 Enolase_C IPR020809 Enolase_CS IPR020811 Enolase_N
PANTHERⁱ	PTHR11902 PTHR11902, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00113 Enolase_C, 1 hit PF03952 Enolase_N, 1 hit
PIRSFⁱ	PIRSF001400 Enolase, 1 hit
PRINTSⁱ	PR00148 ENOLASE
SFLDⁱ	SFLDG00178 enolase, 1 hit SFLDS00001 Enolase, 1 hit
SMARTⁱ	View protein in SMART SM01192 Enolase_C, 1 hit SM01193 Enolase_N, 1 hit
SUPFAMⁱ	SSF51604 SSF51604, 1 hit
TIGRFAMsⁱ	TIGR01060 eno, 1 hit
PROSITEⁱ	View protein in PROSITE PS00164 ENOLASE, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative initiation. Align Add to basket

This entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show all Align All

Isoform alpha-enolase (identifier: P06733-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR 
        60         70         80         90        100
DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT 
       110        120        130        140        150
ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF 
       160        170        180        190        200
NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY 
       210        220        230        240        250
GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE 
       260        270        280        290        300
FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD 
       310        320        330        340        350
WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV 
       360        370        380        390        400
TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR 
       410        420        430 
SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK

Length:434

Mass (Da):47,169

Last modified:January 23, 2007 - v2

Checksum:ⁱA0ED663FCC15ADA5

GO

Isoform MBP-1 (identifier: P06733-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-93: Missing.

« Hide

        10         20         30         40         50
MIEMDGTENK SKFGANAILG VSLAVCKAGA VEKGVPLYRH IADLAGNSEV 
        60         70         80         90        100
ILPVPAFNVI NGGSHAGNKL AMQEFMILPV GAANFREAMR IGAEVYHNLK 
       110        120        130        140        150
NVIKEKYGKD ATNVGDEGGF APNILENKEG LELLKTAIGK AGYTDKVVIG 
       160        170        180        190        200
MDVAASEFFR SGKYDLDFKS PDDPSRYISP DQLADLYKSF IKDYPVVSIE 
       210        220        230        240        250
DPFDQDDWGA WQKFTASAGI QVVGDDLTVT NPKRIAKAVN EKSCNCLLLK 
       260        270        280        290        300
VNQIGSVTES LQACKLAQAN GWGVMVSHRS GETEDTFIAD LVVGLCTGQI 
       310        320        330        340 
KTGAPCRSER LAKYNQLLRI EEELGSKAKF AGRNFRNPLA K

Note: It is uncertain whether the alternative initiation site is at Met-94 or at Met-97.

Show »

Length:341

Mass (Da):36,928

Checksum:ⁱ96D437CF21772928

GO

Computationally mapped potential isoform sequencesⁱ

There are 8 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A0A2R8Y6G6	A0A2R8Y6G6_HUMAN	Alpha-enolase Alpha-enolase	ENO1	434	Annotation score:
K7EM90	K7EM90_HUMAN	Alpha-enolase Alpha-enolase	ENO1	195	Annotation score:
A0A2R8YEM5	A0A2R8YEM5_HUMAN	Alpha-enolase Alpha-enolase	ENO1	171	Annotation score:
A0A2R8Y879	A0A2R8Y879_HUMAN	Alpha-enolase Alpha-enolase	ENO1	125	Annotation score:
K7ERS8	K7ERS8_HUMAN	Alpha-enolase Alpha-enolase	ENO1	83	Annotation score:
A0A2R8YEG5	A0A2R8YEG5_HUMAN	Alpha-enolase Alpha-enolase	ENO1	101	Annotation score:
A0A2R8Y798	A0A2R8Y798_HUMAN	Alpha-enolase Alpha-enolase	ENO1	70	Annotation score:
A0A2R8Y6I8	A0A2R8Y6I8_HUMAN	Alpha-enolase Alpha-enolase	ENO1	33	Annotation score:

Sequence cautionⁱ

The sequence AAA35698 differs from that shown. Sequencing errors.Curated

The sequence AAA35698 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	55	T → A in CAD97642 (PubMed:17974005).Curated	1
Sequence conflictⁱ	78	V → A in BAD96237 (Ref. 7) Curated	1
Sequence conflictⁱ	187	E → G in CAD97642 (PubMed:17974005).Curated	1
Sequence conflictⁱ	199	K → R in BAD96912 (Ref. 7) Curated	1
Sequence conflictⁱ	252	F → S in CAA59331 (PubMed:8824716).Curated	1
Sequence conflictⁱ	310	S → I in CAD97642 (PubMed:17974005).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_025172	177	N → K1 PublicationCorresponds to variant dbSNP:rs11544513Ensembl.		1
Natural variantⁱVAR_048936	325	P → Q. Corresponds to variant dbSNP:rs11544514Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_018725	1 – 93	Missing in isoform MBP-1. 1 PublicationAdd BLAST		93

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M14328 mRNA Translation: AAA52387.1 X16288, X16289, X16290 Genomic DNA Translation: CAA34360.1 M55914 mRNA Translation: AAA35698.1 Frameshift. X84907 mRNA Translation: CAA59331.1 BT007163 mRNA Translation: AAP35827.1 AK315417 mRNA Translation: BAG37806.1 AL833741 mRNA Translation: CAH56247.1 BX537400 mRNA Translation: CAD97642.1 AK222517 mRNA Translation: BAD96237.1 AK223192 mRNA Translation: BAD96912.1 DQ056744 Genomic DNA Translation: AAY43128.1 AL139415 Genomic DNA Translation: CAC42425.1 CH471130 Genomic DNA Translation: EAW71604.1 BC001810 mRNA Translation: AAH01810.1 BC004325 mRNA Translation: AAH04325.1 BC004458 mRNA Translation: AAH04458.1 BC009218 mRNA Translation: AAH09218.2 BC009912 mRNA Translation: AAH09912.1 BC011130 mRNA Translation: AAH11130.1 BC015641 mRNA Translation: AAH15641.1 BC021166 mRNA Translation: AAH21166.2 BC022545 mRNA Translation: AAH22545.1 BC027725 mRNA Translation: AAH27725.1 BC050642 mRNA Translation: AAH50642.1 U88968 mRNA Translation: AAC39935.1 AF035286 mRNA Translation: AAB88178.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS97.1 [P06733-1]
PIRⁱ	A39579 S11696 A29170
NCBI Reference Sequences More...RefSeqⁱ	NP_001188412.1, NM_001201483.1 [P06733-2] NP_001419.1, NM_001428.3 [P06733-1]
UniGeneⁱ	Hs.517145

Genome annotation databases

Ensemblⁱ	ENST00000234590; ENSP00000234590; ENSG00000074800 [P06733-1] ENST00000646539; ENSP00000493526; ENSG00000074800 [P06733-1]
GeneIDⁱ	2023
KEGGⁱ	hsa:2023
UCSC genome browser More...UCSCⁱ	uc001apj.3 human [P06733-1]

Keywords - Coding sequence diversityⁱ

Alternative initiation, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P06733	Enolase 1, (Alpha), isoform CRA_a		HUMAN		434	UniRef100_P06733
Alpha-enolase		PANTR		434
Alpha-enolase		PONAB		434
Uncharacterized protein		PANPA		434
Enolase 1		GORGO		434
+1
P06733-2	C-myc promoter-binding protein 1		HUMAN		338	UniRef100_P06733-2
Uncharacterized protein		MACFA		434
Uncharacterized protein		CERAT		434
Uncharacterized protein		MACNE		377
UPI000DC15FAC		THEGE		448

Protein	Similar proteins		Species	Score	Length	Source
P06733	Alpha-enolase		PONAB		434	UniRef90_P06733
Alpha-enolase		MACFA		434
Alpha-enolase		BOVIN		434
Enolase 1, (Alpha), isoform CRA_a		HUMAN		434
Alpha-enolase		PANTR		434
+99
P06733-2	Alpha-enolase		RAT		434	UniRef90_P06733-2
Alpha-enolase		SCEUN		434
Alpha-enolase		MOUSE		434
Alpha-enolase		TRASE		434
Alpha-enolase	)	CHICK		434
+332

Protein	Similar proteins		Species	Score	Length	Source
P06733	Alpha-enolase		BOVIN		434	UniRef50_P06733
Alpha-enolase		PONAB		434
Alpha-enolase		MACFA		434
Beta-enolase		RAT		434
Beta-enolase		PIG		434
+268
P06733-2	Alpha-enolase		TRASE		434	UniRef50_P06733-2
Alpha-enolase		SCEUN		434
Alpha-enolase		RAT		434
Alpha-enolase		MOUSE		434
Alpha-enolase	)	CHICK		434
+1349

Cross-referencesⁱ

Web resourcesⁱ

NIEHS-SNPs

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M14328 mRNA Translation: AAA52387.1 X16288, X16289, X16290 Genomic DNA Translation: CAA34360.1 M55914 mRNA Translation: AAA35698.1 Frameshift. X84907 mRNA Translation: CAA59331.1 BT007163 mRNA Translation: AAP35827.1 AK315417 mRNA Translation: BAG37806.1 AL833741 mRNA Translation: CAH56247.1 BX537400 mRNA Translation: CAD97642.1 AK222517 mRNA Translation: BAD96237.1 AK223192 mRNA Translation: BAD96912.1 DQ056744 Genomic DNA Translation: AAY43128.1 AL139415 Genomic DNA Translation: CAC42425.1 CH471130 Genomic DNA Translation: EAW71604.1 BC001810 mRNA Translation: AAH01810.1 BC004325 mRNA Translation: AAH04325.1 BC004458 mRNA Translation: AAH04458.1 BC009218 mRNA Translation: AAH09218.2 BC009912 mRNA Translation: AAH09912.1 BC011130 mRNA Translation: AAH11130.1 BC015641 mRNA Translation: AAH15641.1 BC021166 mRNA Translation: AAH21166.2 BC022545 mRNA Translation: AAH22545.1 BC027725 mRNA Translation: AAH27725.1 BC050642 mRNA Translation: AAH50642.1 U88968 mRNA Translation: AAC39935.1 AF035286 mRNA Translation: AAB88178.1
CCDSⁱ	CCDS97.1 [P06733-1]
PIRⁱ	A39579 S11696 A29170
RefSeqⁱ	NP_001188412.1, NM_001201483.1 [P06733-2] NP_001419.1, NM_001428.3 [P06733-1]
UniGeneⁱ	Hs.517145

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2PSN	X-ray	2.20	A/B/C/D	1-434	[»]
	3B97	X-ray	2.20	A/B/C/D	2-434	[»]
	5JLZ	X-ray	1.99	E/F	26-40	[»]
	5LAX	X-ray	2.60	E/F	26-40	[»]
	5NI9	X-ray	1.33	C	326-340	[»]
	5NIG	X-ray	1.35	C	326-340	[»]
ProteinModelPortalⁱ	P06733
SMRⁱ	P06733
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	108338, 296 interactors
IntActⁱ	P06733, 90 interactors
MINTⁱ	P06733
STRINGⁱ	9606.ENSP00000234590

Chemistry databases

BindingDBⁱ	P06733
ChEMBLⁱ	CHEMBL3298

Protein family/group databases

MoonDBⁱ	P06733 Predicted
MoonProtⁱ	P06733

PTM databases

iPTMnetⁱ	P06733
PhosphoSitePlusⁱ	P06733
SwissPalmⁱ	P06733

Polymorphism and mutation databases

BioMutaⁱ	ENO1
DMDMⁱ	119339

2D gel databases

DOSAC-COBS-2DPAGEⁱ	P06733
OGPⁱ	P06733
REPRODUCTION-2DPAGEⁱ	IPI00465248 P06733
SWISS-2DPAGEⁱ	P06733
UCD-2DPAGEⁱ	P06733

Proteomic databases

EPDⁱ	P06733
MaxQBⁱ	P06733
PaxDbⁱ	P06733
PeptideAtlasⁱ	P06733
PRIDEⁱ	P06733
ProteomicsDBⁱ	51919 51920 [P06733-2]
TopDownProteomicsⁱ	P06733-1 [P06733-1] P06733-2 [P06733-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	2023
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000234590; ENSP00000234590; ENSG00000074800 [P06733-1] ENST00000646539; ENSP00000493526; ENSG00000074800 [P06733-1]
GeneIDⁱ	2023
KEGGⁱ	hsa:2023
UCSCⁱ	uc001apj.3 human [P06733-1]

Organism-specific databases

CTDⁱ	2023
DisGeNETⁱ	2023
EuPathDBⁱ	HostDB:ENSG00000074800.13
GeneCardsⁱ	ENO1
HGNCⁱ	HGNC:3350 ENO1
HPAⁱ	CAB018614 CAB069394 HPA068284 HPA068721
MIMⁱ	172430 gene
neXtProtⁱ	NX_P06733
OpenTargetsⁱ	ENSG00000074800
PharmGKBⁱ	PA27786
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG2670 Eukaryota COG0148 LUCA
GeneTreeⁱ	ENSGT00910000144064
HOVERGENⁱ	HBG000067
InParanoidⁱ	P06733
KOⁱ	K01689
OMAⁱ	EFMIIPV
OrthoDBⁱ	EOG091G07NH
PhylomeDBⁱ	P06733
TreeFamⁱ	TF300391

Enzyme and pathway databases

UniPathwayⁱ	UPA00109;UER00187
BioCycⁱ	MetaCyc:ENSG00000074800-MONOMER
BRENDAⁱ	4.2.1.11 2681
Reactomeⁱ	R-HSA-70171 Glycolysis R-HSA-70263 Gluconeogenesis
SABIO-RKⁱ	P06733
SIGNORⁱ	P06733

Miscellaneous databases

ChiTaRSⁱ	ENO1 human
EvolutionaryTraceⁱ	P06733
GeneWikiⁱ	Alpha-enolase
GenomeRNAiⁱ	2023
PMAP-CutDBⁱ	P06733
Protein Ontology More...PROⁱ	PR:P06733
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000074800 Expressed in 231 organ(s), highest expression level in cortex of kidney
ExpressionAtlasⁱ	P06733 baseline and differential
Genevisibleⁱ	P06733 HS

Family and domain databases

CDDⁱ	cd03313 enolase, 1 hit
Gene3Dⁱ	3.20.20.120, 1 hit 3.30.390.10, 1 hit
HAMAPⁱ	MF_00318 Enolase, 1 hit
InterProⁱ	View protein in InterPro IPR000941 Enolase IPR036849 Enolase-like_C_sf IPR029017 Enolase-like_N IPR034390 Enolase-like_superfamily IPR020810 Enolase_C IPR020809 Enolase_CS IPR020811 Enolase_N
PANTHERⁱ	PTHR11902 PTHR11902, 1 hit
Pfamⁱ	View protein in Pfam PF00113 Enolase_C, 1 hit PF03952 Enolase_N, 1 hit
PIRSFⁱ	PIRSF001400 Enolase, 1 hit
PRINTSⁱ	PR00148 ENOLASE
SFLDⁱ	SFLDG00178 enolase, 1 hit SFLDS00001 Enolase, 1 hit
SMARTⁱ	View protein in SMART SM01192 Enolase_C, 1 hit SM01193 Enolase_N, 1 hit
SUPFAMⁱ	SSF51604 SSF51604, 1 hit
TIGRFAMsⁱ	TIGR01060 eno, 1 hit
PROSITEⁱ	View protein in PROSITE PS00164 ENOLASE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ENOA_HUMAN
Accessionⁱ	P06733Primary (citable) accession number: P06733 Secondary accession number(s): B2RD59 Q9UM55
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
	Last sequence update:	January 23, 2007
	Last modified:	November 7, 2018
	This is version 228 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - P06733 (ENOA_HUMAN)

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Alpha-enolase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

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<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

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<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

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Keywords - PTMi

Proteomic databases

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<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

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<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

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<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

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<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

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Sequence databases

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Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

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Functionⁱ

pH dependenceⁱ

Pathwayⁱ: glycolysis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ