UniProtKB - P06725 (PP65_HCMVA)
Protein
65 kDa phosphoprotein
Gene
UL83
Organism
Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Status
Functioni
Counteracts the host antiviral immune response when activated and phosphorylated, by preventing IRF3 from entering the nucleus. Also participates in the transactivation of viral major immediate-early genes by the recruitment of host IFI16 to the promoters pf these genes.2 Publications
GO - Biological processi
- pathogenesis Source: InterPro
- regulation of immune response Source: Reactome
- suppression by virus of host IRF3 activity Source: UniProtKB-KW
Keywordsi
Biological process | Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host RLR pathway by virus, Viral immunoevasion |
Enzyme and pathway databases
Reactomei | R-HSA-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell |
Names & Taxonomyi
Protein namesi | Recommended name: 65 kDa phosphoproteinShort name: pp65 Alternative name(s): 65 kDa matrix phosphoprotein Phosphoprotein UL83 Tegument protein UL83 |
Gene namesi | Name:UL83 |
Organismi | Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5) |
Taxonomic identifieri | 10360 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Peploviricota › Herviviricetes › Herpesvirales › Herpesviridae › Betaherpesvirinae › Cytomegalovirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion tegument Curated
- Host nucleus 2 Publications
- Host cytoplasm 1 Publication Note: As part of the incoming virion, pp65 is targeted to the nucleus immediately after infection (PubMed:7815485). The newly synthesized pp65 is observed in the nucleus until some time after 48 hours postinfection (PubMed:17715235). Thereafter, pp65 is probably exported and accumulates in the cytoplasm (PubMed:17715235). Also found in dense bodies (PubMed:7815485).
GO - Cellular componenti
- host cell cytoplasm Source: UniProtKB-SubCell
- host cell nucleus Source: UniProtKB-SubCell
- viral tegument Source: Reactome
Keywords - Cellular componenti
Host cytoplasm, Host nucleus, Virion, Virion tegumentPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000116292 | 1 – 561 | 65 kDa phosphoproteinAdd BLAST | 561 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 472 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylation may play a role in the localization of the protein.
Keywords - PTMi
PhosphoproteinInteractioni
Subunit structurei
Interacts with host NCL/nucleolin.
Interacts with host IFI16.
2 PublicationsBinary interactionsi
P06725
With | #Exp. | IntAct |
---|---|---|
CHEK2 [O96017] from Homo sapiens. | 2 | EBI-9545359,EBI-1180783 |
Protein-protein interaction databases
DIPi | DIP-61111N |
IntActi | P06725, 4 interactors |
Structurei
3D structure databases
SMRi | P06725 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06725 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 537 – 560 | Bipartite nuclear localization signalCuratedAdd BLAST | 24 |
Sequence similaritiesi
Belongs to the herpesviridae pp65 family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR036157, dUTPase-like_sf IPR008649, Herpes_UL82/UL83 |
Pfami | View protein in Pfam PF05784, Herpes_UL82_83, 1 hit |
SUPFAMi | SSF51283, SSF51283, 1 hit |
i Sequence
Sequence statusi: Complete.
P06725-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESRGRRCPE MISVLGPISG HVLKAVFSRG DTPVLPHETR LLQTGIHVRV
60 70 80 90 100
SQPSLILVSQ YTPDSTPCHR GDNQLQVQHT YFTGSEVENV SVNVHNPTGR
110 120 130 140 150
SICPSQEPMS IYVYALPLKM LNIPSINVHH YPSAAERKHR HLPVADAVIH
160 170 180 190 200
ASGKQMWQAR LTVSGLAWTR QQNQWKEPDV YYTSAFVFPT KDVALRHVVC
210 220 230 240 250
AHELVCSMEN TRATKMQVIG DQYVKVYLES FCEDVPSGKL FMHVTLGSDV
260 270 280 290 300
EEDLTMTRNP QPFMRPHERN GFTVLCPKNM IIKPGKISHI MLDVAFTSHE
310 320 330 340 350
HFGLLCPKSI PGLSISGNLL MNGQQIFLEV QAIRETVELR QYDPVAALFF
360 370 380 390 400
FDIDLLLQRG PQYSEHPTFT SQYRIQGKLE YRHTWDRHDE GAAQGDDDVW
410 420 430 440 450
TSGSDSDEEL VTTERKTPRV TGGGAMAGAS TSAGRKRKSA SSATACTSGV
460 470 480 490 500
MTRGRLKAES TVAPEEDTDE DSDNEIHNPA VFTWPPWQAG ILARNLVPMV
510 520 530 540 550
ATVQGQNLKY QEFFWDANDI YRIFAELEGV WQPAAQPKRR RHRQDALPGP
560
CIASTPKKHR G
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 94 – 106 | Missing in AAA45996 (PubMed:2161319).CuratedAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15120 Genomic DNA Translation: AAA45996.1 Sequence problems. X17403 Genomic DNA Translation: CAA35357.1 K02531 Genomic DNA Translation: AAA45983.1 BK000394 Genomic DNA Translation: DAA00180.1 |
PIRi | A26793, WMBE65 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15120 Genomic DNA Translation: AAA45996.1 Sequence problems. X17403 Genomic DNA Translation: CAA35357.1 K02531 Genomic DNA Translation: AAA45983.1 BK000394 Genomic DNA Translation: DAA00180.1 |
PIRi | A26793, WMBE65 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2X4R | X-ray | 2.30 | C/F | 495-503 | [»] | |
2X4T | X-ray | 2.30 | C/F | 495-503 | [»] | |
3GSO | X-ray | 1.60 | P | 495-503 | [»] | |
SMRi | P06725 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-61111N |
IntActi | P06725, 4 interactors |
Protocols and materials databases
ABCDi | P06725, 8 sequenced antibodies |
Enzyme and pathway databases
Reactomei | R-HSA-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell |
Miscellaneous databases
EvolutionaryTracei | P06725 |
Family and domain databases
InterProi | View protein in InterPro IPR036157, dUTPase-like_sf IPR008649, Herpes_UL82/UL83 |
Pfami | View protein in Pfam PF05784, Herpes_UL82_83, 1 hit |
SUPFAMi | SSF51283, SSF51283, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PP65_HCMVA | |
Accessioni | P06725Primary (citable) accession number: P06725 Secondary accession number(s): Q7M6K8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | July 1, 1989 | |
Last modified: | December 2, 2020 | |
This is version 91 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families