UniProtKB - P06721 (METC_ECOLI)
Protein
Cystathionine beta-lyase MetC
Gene
metC
Organism
Escherichia coli (strain K12)
Status
Functioni
Primarily catalyzes the cleavage of cystathionine to homocysteine, pyruvate and ammonia during methionine biosynthesis (PubMed:7049234). Also exhibits cysteine desulfhydrase activity, producing sulfide from cysteine (PubMed:12883870). In addition, under certain growth conditions, exhibits significant alanine racemase coactivity (PubMed:21193606).3 Publications
Catalytic activityi
Cofactori
pyridoxal 5'-phosphate3 Publications
Activity regulationi
L-cysteine inhibits cystathionine beta-lyase activity competitively (PubMed:7049234). Inhibited by aminoethoxyvinylglycine (AVG) (PubMed:9376370).2 Publications
Kineticsi
- KM=0.04 mM for L-cystathionine1 Publication
- KM=0.25 mM for L-cystine1 Publication
- Vmax=249 µmol/min/mg enzyme with L-cystathionine as substrate1 Publication
- Vmax=263 µmol/min/mg enzyme with L-cystine as substrate1 Publication
pH dependencei
Optimum pH is 8.0-9.0.1 Publication
: L-methionine biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from L-cystathionine.CuratedProteins known to be involved in this subpathway in this organism are:
- Cystathionine beta-lyase MetC (metC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from L-cystathionine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.
GO - Molecular functioni
- alanine racemase activity Source: EcoCyc
- cystathionine beta-lyase activity Source: EcoCyc
- cysteine-S-conjugate beta-lyase activity Source: UniProtKB-EC
- identical protein binding Source: EcoCyc
- L-cysteine desulfhydrase activity Source: EcoCyc
- pyridoxal phosphate binding Source: EcoCyc
GO - Biological processi
- L-cysteine catabolic process to pyruvate Source: EcoCyc
- methionine biosynthetic process Source: EcoCyc
- protein homotetramerization Source: EcoCyc
- sulfur compound metabolic process Source: GO_Central
- transsulfuration Source: InterPro
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Methionine biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:CYSTATHIONINE-BETA-LYASE-MONOMER MetaCyc:CYSTATHIONINE-BETA-LYASE-MONOMER |
BRENDAi | 4.4.1.8, 2026 |
UniPathwayi | UPA00051;UER00078 |
Names & Taxonomyi
Protein namesi | Recommended name: Cystathionine beta-lyase MetC1 Publication (EC:4.4.1.131 Publication)Short name: CBL1 Publication Short name: CL1 Publication Alternative name(s): Beta-cystathionase MetC1 Publication Cysteine lyase MetC Cysteine-S-conjugate beta-lyase MetC |
Gene namesi | Name:metC1 Publication Ordered Locus Names:b3008, JW2975 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Disruption of the gene increases the amounts of L-cysteine and L-cystine produced after 72 hours of cultivation.1 Publication
Chemistry databases
ChEMBLi | CHEMBL1075079 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000114768 | 2 – 395 | Cystathionine beta-lyase MetCAdd BLAST | 394 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 210 | N6-(pyridoxal phosphate)lysine2 Publications | 1 |
Proteomic databases
jPOSTi | P06721 |
PaxDbi | P06721 |
PRIDEi | P06721 |
Interactioni
Subunit structurei
Homotetramer; dimer of dimers.
1 PublicationGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261419, 41 interactors |
DIPi | DIP-10193N |
IntActi | P06721, 6 interactors |
STRINGi | 511145.b3008 |
Chemistry databases
BindingDBi | P06721 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P06721 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06721 |
Family & Domainsi
Sequence similaritiesi
Belongs to the trans-sulfuration enzymes family.Curated
Phylogenomic databases
eggNOGi | COG0626, Bacteria |
HOGENOMi | CLU_018986_5_1_6 |
InParanoidi | P06721 |
PhylomeDBi | P06721 |
Family and domain databases
CDDi | cd00614, CGS_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000277, Cys/Met-Metab_PyrdxlP-dep_enz IPR006233, Cys_b_lyase_bac IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR43500, PTHR43500, 1 hit |
Pfami | View protein in Pfam PF01053, Cys_Met_Meta_PP, 1 hit |
PIRSFi | PIRSF001434, CGS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR01324, cysta_beta_ly_B, 1 hit |
PROSITEi | View protein in PROSITE PS00868, CYS_MET_METAB_PP, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P06721-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADKKLDTQL VNAGRSKKYT LGAVNSVIQR ASSLVFDSVE AKKHATRNRA
60 70 80 90 100
NGELFYGRRG TLTHFSLQQA MCELEGGAGC VLFPCGAAAV ANSILAFIEQ
110 120 130 140 150
GDHVLMTNTA YEPSQDFCSK ILSKLGVTTS WFDPLIGADI VKHLQPNTKI
160 170 180 190 200
VFLESPGSIT MEVHDVPAIV AAVRSVVPDA IIMIDNTWAA GVLFKALDFG
210 220 230 240 250
IDVSIQAATK YLVGHSDAMI GTAVCNARCW EQLRENAYLM GQMVDADTAY
260 270 280 290 300
ITSRGLRTLG VRLRQHHESS LKVAEWLAEH PQVARVNHPA LPGSKGHEFW
310 320 330 340 350
KRDFTGSSGL FSFVLKKKLN NEELANYLDN FSLFSMAYSW GGYESLILAN
360 370 380 390
QPEHIAAIRP QGEIDFSGTL IRLHIGLEDV DDLIADLDAG FARIV
Sequence cautioni
The sequence AAA69175 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12858 Genomic DNA Translation: AAA24158.1 U28377 Genomic DNA Translation: AAA69175.1 Different initiation. U00096 Genomic DNA Translation: AAC76044.1 AP009048 Genomic DNA Translation: BAE77065.1 |
PIRi | A25153, WZECCB |
RefSeqi | NP_417481.1, NC_000913.3 WP_001301079.1, NZ_SSZK01000023.1 |
Genome annotation databases
EnsemblBacteriai | AAC76044; AAC76044; b3008 BAE77065; BAE77065; BAE77065 |
GeneIDi | 946240 |
KEGGi | ecj:JW2975 eco:b3008 |
PATRICi | fig|511145.12.peg.3102 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12858 Genomic DNA Translation: AAA24158.1 U28377 Genomic DNA Translation: AAA69175.1 Different initiation. U00096 Genomic DNA Translation: AAC76044.1 AP009048 Genomic DNA Translation: BAE77065.1 |
PIRi | A25153, WZECCB |
RefSeqi | NP_417481.1, NC_000913.3 WP_001301079.1, NZ_SSZK01000023.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CL1 | X-ray | 1.83 | A/B | 1-395 | [»] | |
1CL2 | X-ray | 2.20 | A/B | 1-395 | [»] | |
2FQ6 | X-ray | 1.78 | A/B | 1-395 | [»] | |
2GQN | X-ray | 1.80 | A/B | 1-395 | [»] | |
4ITG | X-ray | 1.74 | A/B | 1-395 | [»] | |
4ITX | X-ray | 1.61 | A/B | 1-395 | [»] | |
SMRi | P06721 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261419, 41 interactors |
DIPi | DIP-10193N |
IntActi | P06721, 6 interactors |
STRINGi | 511145.b3008 |
Chemistry databases
BindingDBi | P06721 |
ChEMBLi | CHEMBL1075079 |
Proteomic databases
jPOSTi | P06721 |
PaxDbi | P06721 |
PRIDEi | P06721 |
Genome annotation databases
EnsemblBacteriai | AAC76044; AAC76044; b3008 BAE77065; BAE77065; BAE77065 |
GeneIDi | 946240 |
KEGGi | ecj:JW2975 eco:b3008 |
PATRICi | fig|511145.12.peg.3102 |
Organism-specific databases
EchoBASEi | EB0578 |
Phylogenomic databases
eggNOGi | COG0626, Bacteria |
HOGENOMi | CLU_018986_5_1_6 |
InParanoidi | P06721 |
PhylomeDBi | P06721 |
Enzyme and pathway databases
UniPathwayi | UPA00051;UER00078 |
BioCyci | EcoCyc:CYSTATHIONINE-BETA-LYASE-MONOMER MetaCyc:CYSTATHIONINE-BETA-LYASE-MONOMER |
BRENDAi | 4.4.1.8, 2026 |
Miscellaneous databases
EvolutionaryTracei | P06721 |
PROi | PR:P06721 |
Family and domain databases
CDDi | cd00614, CGS_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000277, Cys/Met-Metab_PyrdxlP-dep_enz IPR006233, Cys_b_lyase_bac IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR43500, PTHR43500, 1 hit |
Pfami | View protein in Pfam PF01053, Cys_Met_Meta_PP, 1 hit |
PIRSFi | PIRSF001434, CGS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR01324, cysta_beta_ly_B, 1 hit |
PROSITEi | View protein in PROSITE PS00868, CYS_MET_METAB_PP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | METC_ECOLI | |
Accessioni | P06721Primary (citable) accession number: P06721 Secondary accession number(s): Q2M9J1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 175 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families