UniProtKB - P06720 (AGAL_ECOLI)
Protein
Alpha-galactosidase
Gene
melA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. EC:3.2.1.22
Cofactori
Protein has several cofactor binding sites:- NAD+1 PublicationNote: Binds 1 NAD+ per subunit.1 Publication
- Mn2+1 PublicationNote: Binds 1 Mn2+ ion per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 151 | SubstrateBy similarity | 1 | |
Metal bindingi | 173 | ManganeseBy similarity | 1 | |
Active sitei | 174 | Proton donorBy similarity | 1 | |
Metal bindingi | 203 | ManganeseBy similarity | 1 | |
Binding sitei | 287 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 5 – 71 | NADBy similarityAdd BLAST | 67 |
GO - Molecular functioni
- alpha-galactosidase activity Source: EcoCyc
- manganese ion binding Source: EcoCyc
- NAD+ binding Source: EcoCyc
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
- raffinose alpha-galactosidase activity Source: UniProtKB-EC
GO - Biological processi
- melibiose catabolic process Source: EcoCyc
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Manganese, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:ALPHAGALACTOSID-MONOMER MetaCyc:ALPHAGALACTOSID-MONOMER |
Protein family/group databases
CAZyi | GH4, Glycoside Hydrolase Family 4 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:melA Synonyms:mel-7 Ordered Locus Names:b4119, JW4080 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169852 | 1 – 451 | Alpha-galactosidaseAdd BLAST | 451 |
Proteomic databases
jPOSTi | P06720 |
PaxDbi | P06720 |
PRIDEi | P06720 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262685, 8 interactors |
DIPi | DIP-10179N |
IntActi | P06720, 3 interactors |
STRINGi | 511145.b4119 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 4 family.Curated
Phylogenomic databases
eggNOGi | COG1486, Bacteria |
HOGENOMi | CLU_045951_1_1_6 |
InParanoidi | P06720 |
PhylomeDBi | P06720 |
Family and domain databases
InterProi | View protein in InterPro IPR019802, GlycHydrolase_4_CS IPR001088, Glyco_hydro_4 IPR022616, Glyco_hydro_4_C IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR32092, PTHR32092, 1 hit |
Pfami | View protein in Pfam PF02056, Glyco_hydro_4, 1 hit PF11975, Glyco_hydro_4C, 1 hit |
PRINTSi | PR00732, GLHYDRLASE4 |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS01324, GLYCOSYL_HYDROL_F4, 1 hit |
i Sequence
Sequence statusi: Complete.
P06720-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMSAPKITFI GAGSTIFVKN ILGDVFHREA LKTAHIALMD IDPTRLEESH
60 70 80 90 100
IVVRKLMDSA GASGKITCHT QQKEALEDAD FVVVAFQIGG YEPCTVTDFE
110 120 130 140 150
VCKRHGLEQT IADTLGPGGI MRALRTIPHL WQICEDMTEV CPDATMLNYV
160 170 180 190 200
NPMAMNTWAM YARYPHIKQV GLCHSVQGTA EELARDLNID PATLRYRCAG
210 220 230 240 250
INHMAFYLEL ERKTADGSYV NLYPELLAAY EAGQAPKPNI HGNTRCQNIV
260 270 280 290 300
RYEMFKKLGY FVTESSEHFA EYTPWFIKPG REDLIERYKV PLDEYPKRCV
310 320 330 340 350
EQLANWHKEL EEYKKASRID IKPSREYAST IMNAIWTGEP SVIYGNVRND
360 370 380 390 400
GLIDNLPQGC CVEVACLVDA NGIQPTKVGT LPSHLAALMQ TNINVQTLLT
410 420 430 440 450
EAILTENRDR VYHAAMMDPH TAAVLGIDEI YALVDDLIAA HGDWLPGWLH
R
Sequence cautioni
The sequence AAA24149 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04894 Genomic DNA Translation: CAA28581.1 K01490 Genomic DNA Translation: AAA24149.1 Different initiation. U14003 Genomic DNA Translation: AAA97019.1 U00096 Genomic DNA Translation: AAC77080.1 AP009048 Genomic DNA Translation: BAE78121.1 |
PIRi | A26571, GBECAG |
RefSeqi | NP_418543.1, NC_000913.3 WP_000986601.1, NZ_SSZK01000018.1 |
Genome annotation databases
EnsemblBacteriai | AAC77080; AAC77080; b4119 BAE78121; BAE78121; BAE78121 |
GeneIDi | 948636 |
KEGGi | ecj:JW4080 eco:b4119 |
PATRICi | fig|1411691.4.peg.2581 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04894 Genomic DNA Translation: CAA28581.1 K01490 Genomic DNA Translation: AAA24149.1 Different initiation. U14003 Genomic DNA Translation: AAA97019.1 U00096 Genomic DNA Translation: AAC77080.1 AP009048 Genomic DNA Translation: BAE78121.1 |
PIRi | A26571, GBECAG |
RefSeqi | NP_418543.1, NC_000913.3 WP_000986601.1, NZ_SSZK01000018.1 |
3D structure databases
SMRi | P06720 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262685, 8 interactors |
DIPi | DIP-10179N |
IntActi | P06720, 3 interactors |
STRINGi | 511145.b4119 |
Chemistry databases
ChEMBLi | CHEMBL3696 |
Protein family/group databases
CAZyi | GH4, Glycoside Hydrolase Family 4 |
Proteomic databases
jPOSTi | P06720 |
PaxDbi | P06720 |
PRIDEi | P06720 |
Genome annotation databases
EnsemblBacteriai | AAC77080; AAC77080; b4119 BAE78121; BAE78121; BAE78121 |
GeneIDi | 948636 |
KEGGi | ecj:JW4080 eco:b4119 |
PATRICi | fig|1411691.4.peg.2581 |
Organism-specific databases
EchoBASEi | EB0572 |
Phylogenomic databases
eggNOGi | COG1486, Bacteria |
HOGENOMi | CLU_045951_1_1_6 |
InParanoidi | P06720 |
PhylomeDBi | P06720 |
Enzyme and pathway databases
BioCyci | EcoCyc:ALPHAGALACTOSID-MONOMER MetaCyc:ALPHAGALACTOSID-MONOMER |
Miscellaneous databases
PROi | PR:P06720 |
Family and domain databases
InterProi | View protein in InterPro IPR019802, GlycHydrolase_4_CS IPR001088, Glyco_hydro_4 IPR022616, Glyco_hydro_4_C IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR32092, PTHR32092, 1 hit |
Pfami | View protein in Pfam PF02056, Glyco_hydro_4, 1 hit PF11975, Glyco_hydro_4C, 1 hit |
PRINTSi | PR00732, GLHYDRLASE4 |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS01324, GLYCOSYL_HYDROL_F4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AGAL_ECOLI | |
Accessioni | P06720Primary (citable) accession number: P06720 Secondary accession number(s): Q2M6I5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families