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Protein

DNA polymerase III subunit tau

Gene

dnaX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the beta sliding clamp loading complex, which hydrolyzes ATP to load the beta clamp onto primed DNA to form the DNA replication pre-initiation complex (PubMed:2040637). DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3'-5' exonuclease activity. The gamma complex (gamma3,delta,delta') is thought to load beta dimers onto DNA by binding ATP which alters the complex's conformation so it can bind beta sliding clamp dimers and open them at one interface. Primed DNA is recognized, ATP is hydrolyzed releasing the gamma complex and closing the beta sliding clamp ring around the primed DNA (PubMed:9927437).1 Publication
Isoform tau: serves as a scaffold to trimerize the core complex (PubMed:7037770).1 Publication
Isoform gamma: interacts with the delta and delta' subunits to transfer the beta subunit on the DNA (PubMed:9927437). Interacts with ATP, drives ATP-induced conformational changes in the gamma complex that opens the beta sliding clamp ring. After loading of primed DNA ATP is hydrolyzed and the beta sliding clamp ring closes (PubMed:9927437).1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • DNA-dependent DNA replication Source: GO_Central
  • DNA replication Source: EcoliWiki

Keywordsi

Molecular functionDNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:EG10245-MONOMER
MetaCyc:MONOMER0-2383

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit tau (EC:2.7.7.7)
Alternative name(s):
DNA polymerase III subunit gamma
Gene namesi
Name:dnaX
Synonyms:dnaZ, dnaZX
Ordered Locus Names:b0470, JW0459
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10245 dnaX

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi118G → D in dnaX2016(Ts); present in both isoforms, unable to grow at 42 degrees Celsius. 1 Publication1
Mutagenesisi601E → K in dnaX36(Ts); present only in isoform tau, unable to grow at 42 degrees Celsius. 1 Publication1

Chemistry databases

DrugBankiDB02930 Phosphothiophosphoric Acid-Adenylate Ester

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000073602 – 643DNA polymerase III subunit tauAdd BLAST642

Proteomic databases

EPDiP06710
PaxDbiP06710
PRIDEiP06710

Interactioni

Subunit structurei

The DNA polymerase III holoenzyme complex contains at least 10 different subunits organized into 3 functionally essential subassemblies: the Pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The Pol III core (subunits alpha, epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities (PubMed:2040637). The polymerase is tethered to the template via the dimeric beta sliding clamp processivity factor. The clamp-loading complex (also called gamma complex) assembles the beta sliding clamp onto the primed template and plays a central role in the organization and communication at the replication fork. The clamp-loading complex contains delta, delta', psi and chi, and 3 copies of either or both of two different DnaX proteins, gamma and tau. The DNA replisome complex has a single clamp loader (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase (PubMed:20413500, PubMed:22157955). The clamp loader hydrolyzes ATP to assemble the beta processivity factor onto the primed template (PubMed:2040637, PubMed:9927437) and plays a central role in the organization and communication at the replication fork; the minimal complex to load the beta sliding clamp on DNA is delta, delta', gamma (PubMed:9927437).5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4259846, 140 interactors
ComplexPortaliCPX-1926 DNA polymerase III clamp loader complex [P06710-2]
DIPiDIP-9464N
IntActiP06710, 33 interactors
STRINGi316385.ECDH10B_0426

Chemistry databases

BindingDBiP06710

Structurei

Secondary structure

1643
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP06710
SMRiP06710
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06710

Family & Domainsi

Sequence similaritiesi

Belongs to the DnaX/STICHEL family.Curated

Phylogenomic databases

eggNOGiENOG4107QMP Bacteria
COG2812 LUCA
HOGENOMiHOG000083934
InParanoidiP06710
KOiK02343
OMAiFFYQVIV
PhylomeDBiP06710

Family and domain databases

Gene3Di3.30.300.150, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR001270 ClpA/B
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR022001 DNA_pol3_tau_IV
IPR022754 DNA_pol_III_gamma-3
IPR012763 DNA_pol_III_sug/sutau
IPR021029 DNA_pol_III_tau_dom-5
IPR027417 P-loop_NTPase
IPR038249 PolIII_tau_C_sf
PfamiView protein in Pfam
PF12169 DNA_pol3_gamma3, 1 hit
PF12168 DNA_pol3_tau_4, 1 hit
PF12170 DNA_pol3_tau_5, 1 hit
PRINTSiPR00300 CLPPROTEASEA
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02397 dnaX_nterm, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket
Note: The production of the two protein products from this region is due to programmed ribosomal frameshifting. Frameshifting is about 40% efficient.3 Publications
Isoform tau (identifier: P06710-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSYQVLARKW RPQTFADVVG QEHVLTALAN GLSLGRIHHA YLFSGTRGVG
60 70 80 90 100
KTSIARLLAK GLNCETGITA TPCGVCDNCR EIEQGRFVDL IEIDAASRTK
110 120 130 140 150
VEDTRDLLDN VQYAPARGRF KVYLIDEVHM LSRHSFNALL KTLEEPPEHV
160 170 180 190 200
KFLLATTDPQ KLPVTILSRC LQFHLKALDV EQIRHQLEHI LNEEHIAHEP
210 220 230 240 250
RALQLLARAA EGSLRDALSL TDQAIASGDG QVSTQAVSAM LGTLDDDQAL
260 270 280 290 300
SLVEAMVEAN GERVMALINE AAARGIEWEA LLVEMLGLLH RIAMVQLSPA
310 320 330 340 350
ALGNDMAAIE LRMRELARTI PPTDIQLYYQ TLLIGRKELP YAPDRRMGVE
360 370 380 390 400
MTLLRALAFH PRMPLPEPEV PRQSFAPVAP TAVMTPTQVP PQPQSAPQQA
410 420 430 440 450
PTVPLPETTS QVLAARQQLQ RVQGATKAKK SEPAAATRAR PVNNAALERL
460 470 480 490 500
ASVTDRVQAR PVPSALEKAP AKKEAYRWKA TTPVMQQKEV VATPKALKKA
510 520 530 540 550
LEHEKTPELA AKLAAEAIER DPWAAQVSQL SLPKLVEQVA LNAWKEESDN
560 570 580 590 600
AVCLHLRSSQ RHLNNRGAQQ KLAEALSMLK GSTVELTIVE DDNPAVRTPL
610 620 630 640
EWRQAIYEEK LAQARESIIA DNNIQTLRRF FDAELDEESI RPI
Note: Produced by full-length translation of the dnaX gene. This isoform is essential, constructs that express only the gamma isoform are not viable (PubMed:8376347).1 Publication
Length:643
Mass (Da):71,138
Last modified:January 1, 1988 - v1
Checksum:iD2028BD99E375150
GO
Isoform gamma (identifier: P06710-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     431-431: S → E
     432-643: Missing.

Note: Formed by programmed ribosomal frameshifting to a premature stop codon in the -1 frame at codon 430, the last residue is thus Glu and not Ser. Mutants which remove the frameshift are viable, suggesting strongly that gamma is not essential for viability (PubMed:8376347).4 Publications
Show »
Length:431
Mass (Da):47,545
Checksum:i8177A07F31BC86E3
GO

Sequence cautioni

The sequence CAA28175 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042848431S → E in isoform gamma. Curated1
Alternative sequenceiVSP_042849432 – 643Missing in isoform gamma. CuratedAdd BLAST212

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04487 Genomic DNA Translation: CAA28174.1
X04487 Genomic DNA Translation: CAA28175.1 Different initiation.
X04275 Genomic DNA Translation: CAA27827.1
U82664 Genomic DNA Translation: AAB40224.1
U00096 Genomic DNA Translation: AAC73572.1
AP009048 Genomic DNA Translation: BAE76249.1
M38777 Genomic DNA Translation: AAA23457.1
PIRiA25549 DJEC3G
RefSeqiNP_415003.1, NC_000913.3
WP_000122013.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73572; AAC73572; b0470
BAE76249; BAE76249; BAE76249
GeneIDi945105
KEGGiecj:JW0459
eco:b0470
PATRICifig|1411691.4.peg.1806

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04487 Genomic DNA Translation: CAA28174.1
X04487 Genomic DNA Translation: CAA28175.1 Different initiation.
X04275 Genomic DNA Translation: CAA27827.1
U82664 Genomic DNA Translation: AAB40224.1
U00096 Genomic DNA Translation: AAC73572.1
AP009048 Genomic DNA Translation: BAE76249.1
M38777 Genomic DNA Translation: AAA23457.1
PIRiA25549 DJEC3G
RefSeqiNP_415003.1, NC_000913.3
WP_000122013.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JR3X-ray2.70A/B/C1-373[»]
1NJFX-ray2.30A/B/C/D1-243[»]
1NJGX-ray2.20A/B1-243[»]
1XXHX-ray3.45B/C/D/G/H/I1-373[»]
1XXIX-ray4.10B/C/D/G/H/I1-368[»]
2AYANMR-A499-625[»]
3GLFX-ray3.39B/C/D/G/H/I1-373[»]
3GLGX-ray3.25B/C/D/G/H/I1-373[»]
3GLHX-ray3.89B/C/D/G/H/I/L/M/N1-373[»]
3GLIX-ray3.50B/C/D/G/H/I1-373[»]
5FKUelectron microscopy8.34E500-643[»]
5FKVelectron microscopy8.00E500-643[»]
ProteinModelPortaliP06710
SMRiP06710
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259846, 140 interactors
ComplexPortaliCPX-1926 DNA polymerase III clamp loader complex [P06710-2]
DIPiDIP-9464N
IntActiP06710, 33 interactors
STRINGi316385.ECDH10B_0426

Chemistry databases

BindingDBiP06710
DrugBankiDB02930 Phosphothiophosphoric Acid-Adenylate Ester

Proteomic databases

EPDiP06710
PaxDbiP06710
PRIDEiP06710

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73572; AAC73572; b0470
BAE76249; BAE76249; BAE76249
GeneIDi945105
KEGGiecj:JW0459
eco:b0470
PATRICifig|1411691.4.peg.1806

Organism-specific databases

EchoBASEiEB0241
EcoGeneiEG10245 dnaX

Phylogenomic databases

eggNOGiENOG4107QMP Bacteria
COG2812 LUCA
HOGENOMiHOG000083934
InParanoidiP06710
KOiK02343
OMAiFFYQVIV
PhylomeDBiP06710

Enzyme and pathway databases

BioCyciMetaCyc:EG10245-MONOMER
MetaCyc:MONOMER0-2383

Miscellaneous databases

EvolutionaryTraceiP06710
PROiPR:P06710

Family and domain databases

Gene3Di3.30.300.150, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR001270 ClpA/B
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR022001 DNA_pol3_tau_IV
IPR022754 DNA_pol_III_gamma-3
IPR012763 DNA_pol_III_sug/sutau
IPR021029 DNA_pol_III_tau_dom-5
IPR027417 P-loop_NTPase
IPR038249 PolIII_tau_C_sf
PfamiView protein in Pfam
PF12169 DNA_pol3_gamma3, 1 hit
PF12168 DNA_pol3_tau_4, 1 hit
PF12170 DNA_pol3_tau_5, 1 hit
PRINTSiPR00300 CLPPROTEASEA
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02397 dnaX_nterm, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPO3X_ECOLI
AccessioniPrimary (citable) accession number: P06710
Secondary accession number(s): Q2MBV7, Q47721
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 10, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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