UniProtKB - P06710 (DPO3X_ECOLI)
DNA polymerase III subunit tau
dnaX
Functioni
Part of the beta sliding clamp loading complex, which hydrolyzes ATP to load the beta clamp onto primed DNA to form the DNA replication pre-initiation complex (PubMed:2040637).
DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3'-5' exonuclease activity. The gamma complex (gamma3,delta,delta') is thought to load beta dimers onto DNA by binding ATP which alters the complex's conformation so it can bind beta sliding clamp dimers and open them at one interface. Primed DNA is recognized, ATP is hydrolyzed releasing the gamma complex and closing the beta sliding clamp ring around the primed DNA (PubMed:9927437).
1 PublicationServes as a scaffold to trimerize the core complex (PubMed:7037770).
1 PublicationInteracts with the delta and delta' subunits to transfer the beta subunit on the DNA (PubMed:9927437).
Interacts with ATP, drives ATP-induced conformational changes in the gamma complex that opens the beta sliding clamp ring. After loading of primed DNA ATP is hydrolyzed and the beta sliding clamp ring closes (PubMed:9927437).
1 PublicationCatalytic activityi
- EC:2.7.7.7
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 45 – 52 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: EcoliWiki
- DNA binding Source: InterPro
- DNA-directed DNA polymerase activity Source: UniProtKB-KW
- DNA polymerase processivity factor activity Source: EcoliWiki
- identical protein binding Source: IntAct
- nucleoside-triphosphatase activity Source: EcoliWiki
GO - Biological processi
- DNA-dependent DNA replication Source: GO_Central
- DNA replication Source: EcoliWiki
Keywordsi
Molecular function | DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase |
Biological process | DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.6.4.B8, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase III subunit tau (EC:2.7.7.7)Alternative name(s): DNA polymerase III subunit gamma |
Gene namesi | Name:dnaX Synonyms:dnaZ, dnaZX Ordered Locus Names:b0470, JW0459 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 118 | G → D in dnaX2016(Ts); present in both isoforms, unable to grow at 42 degrees Celsius. 1 Publication | 1 | |
Mutagenesisi | 601 | E → K in dnaX36(Ts); present only in isoform tau, unable to grow at 42 degrees Celsius. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB02930, Adenosine 5'-[gamma-thio]triphosphate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000007360 | 2 – 643 | DNA polymerase III subunit tauAdd BLAST | 642 |
Proteomic databases
jPOSTi | P06710 |
PaxDbi | P06710 |
PRIDEi | P06710 |
Interactioni
Subunit structurei
The DNA polymerase III holoenzyme complex contains at least 10 different subunits organized into 3 functionally essential subassemblies: the Pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The Pol III core (subunits alpha, epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities (PubMed:2040637). The polymerase is tethered to the template via the dimeric beta sliding clamp processivity factor. The clamp-loading complex (also called gamma complex) assembles the beta sliding clamp onto the primed template and plays a central role in the organization and communication at the replication fork. The clamp-loading complex contains delta, delta', psi and chi, and 3 copies of either or both of two different DnaX proteins, gamma and tau. The DNA replisome complex has a single clamp loader (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase (PubMed:20413500, PubMed:22157955). The clamp loader hydrolyzes ATP to assemble the beta processivity factor onto the primed template (PubMed:2040637, PubMed:9927437) and plays a central role in the organization and communication at the replication fork; the minimal complex to load the beta sliding clamp on DNA is delta, delta', gamma (PubMed:9927437).
5 PublicationsBinary interactionsi
P06710
Isoform tau [P06710-1]
With | #Exp. | IntAct |
---|---|---|
holA [P28630] | 5 | EBI-6464728,EBI-549153 |
holB [P28631] | 6 | EBI-6464728,EBI-549161 |
dnaX - isoform gamma [P06710-2]
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-2604194,EBI-2604194 |
holA [P28630] | 6 | EBI-2604194,EBI-549153 |
holD [P28632] | 2 | EBI-2604194,EBI-549176 |
mutL [P23367] | 2 | EBI-2604194,EBI-554913 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259846, 140 interactors 849494, 14 interactors |
ComplexPortali | CPX-1926, DNA polymerase III clamp loader complex |
DIPi | DIP-9464N |
IntActi | P06710, 33 interactors |
STRINGi | 511145.b0470 |
Chemistry databases
BindingDBi | P06710 |
Structurei
Secondary structure
3D structure databases
BMRBi | P06710 |
SMRi | P06710 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06710 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 385 – 404 | DisorderedSequence analysisAdd BLAST | 20 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 385 – 400 | Pro residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2812, Bacteria |
HOGENOMi | CLU_006229_6_0_6 |
InParanoidi | P06710 |
PhylomeDBi | P06710 |
Family and domain databases
CDDi | cd18137, HLD_clamp_pol_III_gamma_tau, 1 hit |
Gene3Di | 3.30.300.150, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR001270, ClpA/B IPR008921, DNA_pol3_clamp-load_cplx_C IPR022001, DNA_pol3_tau_IV IPR022754, DNA_pol_III_gamma-3 IPR012763, DNA_pol_III_sug/sutau_N IPR021029, DNA_pol_III_tau_dom-5 IPR045085, HLD_clamp_pol_III_gamma_tau IPR027417, P-loop_NTPase IPR038249, PolIII_tau_V_sf |
Pfami | View protein in Pfam PF12169, DNA_pol3_gamma3, 1 hit PF12168, DNA_pol3_tau_4, 1 hit PF12170, DNA_pol3_tau_5, 1 hit |
PRINTSi | PR00300, CLPPROTEASEA |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF48019, SSF48019, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR02397, dnaX_nterm, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSYQVLARKW RPQTFADVVG QEHVLTALAN GLSLGRIHHA YLFSGTRGVG
60 70 80 90 100
KTSIARLLAK GLNCETGITA TPCGVCDNCR EIEQGRFVDL IEIDAASRTK
110 120 130 140 150
VEDTRDLLDN VQYAPARGRF KVYLIDEVHM LSRHSFNALL KTLEEPPEHV
160 170 180 190 200
KFLLATTDPQ KLPVTILSRC LQFHLKALDV EQIRHQLEHI LNEEHIAHEP
210 220 230 240 250
RALQLLARAA EGSLRDALSL TDQAIASGDG QVSTQAVSAM LGTLDDDQAL
260 270 280 290 300
SLVEAMVEAN GERVMALINE AAARGIEWEA LLVEMLGLLH RIAMVQLSPA
310 320 330 340 350
ALGNDMAAIE LRMRELARTI PPTDIQLYYQ TLLIGRKELP YAPDRRMGVE
360 370 380 390 400
MTLLRALAFH PRMPLPEPEV PRQSFAPVAP TAVMTPTQVP PQPQSAPQQA
410 420 430 440 450
PTVPLPETTS QVLAARQQLQ RVQGATKAKK SEPAAATRAR PVNNAALERL
460 470 480 490 500
ASVTDRVQAR PVPSALEKAP AKKEAYRWKA TTPVMQQKEV VATPKALKKA
510 520 530 540 550
LEHEKTPELA AKLAAEAIER DPWAAQVSQL SLPKLVEQVA LNAWKEESDN
560 570 580 590 600
AVCLHLRSSQ RHLNNRGAQQ KLAEALSMLK GSTVELTIVE DDNPAVRTPL
610 620 630 640
EWRQAIYEEK LAQARESIIA DNNIQTLRRF FDAELDEESI RPI
The sequence of this isoform differs from the canonical sequence as follows:
431-431: S → E
432-643: Missing.
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042848 | 431 | S → E in isoform gamma. Curated | 1 | |
Alternative sequenceiVSP_042849 | 432 – 643 | Missing in isoform gamma. CuratedAdd BLAST | 212 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04487 Genomic DNA Translation: CAA28174.1 X04487 Genomic DNA Translation: CAA28175.1 Different initiation. X04275 Genomic DNA Translation: CAA27827.1 U82664 Genomic DNA Translation: AAB40224.1 U00096 Genomic DNA Translation: AAC73572.1 U00096 Genomic DNA Translation: AYC08179.1 AP009048 Genomic DNA Translation: BAE76249.1 M38777 Genomic DNA Translation: AAA23457.1 |
PIRi | A25549, DJEC3G |
RefSeqi | NP_415003.1, NC_000913.3 WP_000122013.1, NZ_SSUW01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC73572; AAC73572; b0470 AYC08179; AYC08179; b0470 BAE76249; BAE76249; BAE76249 |
GeneIDi | 945105 |
KEGGi | ecj:JW0459 eco:b0470 |
PATRICi | fig|1411691.4.peg.1806 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04487 Genomic DNA Translation: CAA28174.1 X04487 Genomic DNA Translation: CAA28175.1 Different initiation. X04275 Genomic DNA Translation: CAA27827.1 U82664 Genomic DNA Translation: AAB40224.1 U00096 Genomic DNA Translation: AAC73572.1 U00096 Genomic DNA Translation: AYC08179.1 AP009048 Genomic DNA Translation: BAE76249.1 M38777 Genomic DNA Translation: AAA23457.1 |
PIRi | A25549, DJEC3G |
RefSeqi | NP_415003.1, NC_000913.3 WP_000122013.1, NZ_SSUW01000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JR3 | X-ray | 2.70 | A/B/C | 1-373 | [»] | |
1NJF | X-ray | 2.30 | A/B/C/D | 1-243 | [»] | |
1NJG | X-ray | 2.20 | A/B | 1-243 | [»] | |
1XXH | X-ray | 3.45 | B/C/D/G/H/I | 1-373 | [»] | |
1XXI | X-ray | 4.10 | B/C/D/G/H/I | 1-368 | [»] | |
2AYA | NMR | - | A | 499-625 | [»] | |
3GLF | X-ray | 3.39 | B/C/D/G/H/I | 1-373 | [»] | |
3GLG | X-ray | 3.25 | B/C/D/G/H/I | 1-373 | [»] | |
3GLH | X-ray | 3.89 | B/C/D/G/H/I/L/M/N | 1-373 | [»] | |
3GLI | X-ray | 3.50 | B/C/D/G/H/I | 1-373 | [»] | |
5FKU | electron microscopy | 8.34 | E | 500-643 | [»] | |
5FKV | electron microscopy | 8.00 | E | 500-643 | [»] | |
BMRBi | P06710 | |||||
SMRi | P06710 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259846, 140 interactors 849494, 14 interactors |
ComplexPortali | CPX-1926, DNA polymerase III clamp loader complex |
DIPi | DIP-9464N |
IntActi | P06710, 33 interactors |
STRINGi | 511145.b0470 |
Chemistry databases
BindingDBi | P06710 |
DrugBanki | DB02930, Adenosine 5'-[gamma-thio]triphosphate |
Proteomic databases
jPOSTi | P06710 |
PaxDbi | P06710 |
PRIDEi | P06710 |
Genome annotation databases
EnsemblBacteriai | AAC73572; AAC73572; b0470 AYC08179; AYC08179; b0470 BAE76249; BAE76249; BAE76249 |
GeneIDi | 945105 |
KEGGi | ecj:JW0459 eco:b0470 |
PATRICi | fig|1411691.4.peg.1806 |
Organism-specific databases
EchoBASEi | EB0241 |
Phylogenomic databases
eggNOGi | COG2812, Bacteria |
HOGENOMi | CLU_006229_6_0_6 |
InParanoidi | P06710 |
PhylomeDBi | P06710 |
Enzyme and pathway databases
BRENDAi | 3.6.4.B8, 2026 |
Miscellaneous databases
EvolutionaryTracei | P06710 |
PROi | PR:P06710 |
Family and domain databases
CDDi | cd18137, HLD_clamp_pol_III_gamma_tau, 1 hit |
Gene3Di | 3.30.300.150, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR001270, ClpA/B IPR008921, DNA_pol3_clamp-load_cplx_C IPR022001, DNA_pol3_tau_IV IPR022754, DNA_pol_III_gamma-3 IPR012763, DNA_pol_III_sug/sutau_N IPR021029, DNA_pol_III_tau_dom-5 IPR045085, HLD_clamp_pol_III_gamma_tau IPR027417, P-loop_NTPase IPR038249, PolIII_tau_V_sf |
Pfami | View protein in Pfam PF12169, DNA_pol3_gamma3, 1 hit PF12168, DNA_pol3_tau_4, 1 hit PF12170, DNA_pol3_tau_5, 1 hit |
PRINTSi | PR00300, CLPPROTEASEA |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF48019, SSF48019, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR02397, dnaX_nterm, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPO3X_ECOLI | |
Accessioni | P06710Primary (citable) accession number: P06710 Secondary accession number(s): A0A385XJC4, Q2MBV7, Q47721 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 202 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families