UniProtKB - P06702 (S10A9_HUMAN)
Protein S100-A9
S100A9
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 20 | ZincCurated | 1 | |
Metal bindingi | 30 | ZincCurated | 1 | |
Metal bindingi | 91 | ZincCurated | 1 | |
Metal bindingi | 95 | ZincCurated | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 23 – 36 | 1; low affinityAdd BLAST | 14 | |
Calcium bindingi | 67 – 78 | 2; high affinityCuratedAdd BLAST | 12 |
GO - Molecular functioni
- antioxidant activity Source: UniProtKB-KW
- arachidonic acid binding Source: UniProtKB
- calcium-dependent protein binding Source: GO_Central
- calcium ion binding Source: GO_Central
- microtubule binding Source: UniProtKB
- RAGE receptor binding Source: UniProtKB
- Toll-like receptor 4 binding Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
- antimicrobial humoral response Source: Reactome
- apoptotic process Source: UniProtKB-KW
- astrocyte development Source: GO_Central
- autocrine signaling Source: GO_Central
- autophagy Source: UniProtKB
- cell-cell signaling Source: ProtInc
- chemokine production Source: UniProtKB
- chronic inflammatory response Source: GO_Central
- cytokine production Source: UniProtKB
- defense response to bacterium Source: UniProtKB
- defense response to fungus Source: UniProtKB
- inflammatory response Source: ProtInc
- innate immune response Source: UniProtKB-KW
- leukocyte migration involved in inflammatory response Source: UniProtKB
- modulation of process of other organism Source: UniProtKB
- neutrophil aggregation Source: UniProtKB
- neutrophil chemotaxis Source: UniProtKB
- neutrophil degranulation Source: Reactome
- peptidyl-cysteine S-nitrosylation Source: GO_Central
- peptidyl-cysteine S-trans-nitrosylation Source: UniProtKB
- positive regulation of cell growth Source: UniProtKB
- positive regulation of inflammatory response Source: UniProtKB
- positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
- positive regulation of neuron projection development Source: ARUK-UCL
- positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
- regulation of cytoskeleton organization Source: UniProtKB
- regulation of integrin biosynthetic process Source: GO_Central
- response to lipopolysaccharide Source: GO_Central
- sequestering of zinc ion Source: UniProtKB
- toll-like receptor signaling pathway Source: Reactome
Keywordsi
Molecular function | Antimicrobial, Antioxidant |
Biological process | Apoptosis, Autophagy, Chemotaxis, Immunity, Inflammatory response, Innate immunity |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | P06702 |
Reactomei | R-HSA-5668599, RHO GTPases Activate NADPH Oxidases R-HSA-5686938, Regulation of TLR by endogenous ligand R-HSA-6798695, Neutrophil degranulation R-HSA-6799990, Metal sequestration by antimicrobial proteins |
SIGNORi | P06702 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein S100-A9Alternative name(s): Calgranulin-B Calprotectin L1H subunit Leukocyte L1 complex heavy chain Migration inhibitory factor-related protein 14 Short name: MRP-14 Short name: p14 S100 calcium-binding protein A9 |
Gene namesi | Name:S100A9 Synonyms:CAGB, CFAG, MRP14 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10499, S100A9 |
MIMi | 123886, gene |
neXtProti | NX_P06702 |
VEuPathDBi | HostDB:ENSG00000163220.10 |
Subcellular locationi
Extracellular region or secreted
Plasma membrane
Cytoskeleton
Other locations
Note: Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alternative pathway.
Cytoskeleton
- cytoskeleton Source: UniProtKB
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- collagen-containing extracellular matrix Source: BHF-UCL
- extracellular exosome Source: UniProtKB
- extracellular region Source: BHF-UCL
- extracellular space Source: UniProtKB
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cell junction Source: HPA
- cytoplasm Source: UniProtKB
- secretory granule lumen Source: Reactome
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, Membrane, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 3 | C → A: Disrupts interaction with NOS2 and inhibits LDL(ox)-induced GAPDH S-nitrosylation; no effect on interaction with S100A8. 1 Publication | 1 | |
Mutagenesisi | 36 | E → Q: Loss of resistance to bacterial invasion; when associated with Q-78. 1 Publication | 1 | |
Mutagenesisi | 63 | M → A: Loss of antifungal activity. 1 Publication | 1 | |
Mutagenesisi | 78 | E → Q: Loss of resistance to bacterial invasion; when associated with Q-36. 1 Publication | 1 | |
Mutagenesisi | 81 | M → A: No effect on antifungal activity. 1 Publication | 1 | |
Mutagenesisi | 83 | M → A: Loss of antifungal activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6280 |
OpenTargetsi | ENSG00000163220 |
PharmGKBi | PA34911 |
Miscellaneous databases
Pharosi | P06702, Tbio |
Chemistry databases
DrugBanki | DB01373, Calcium DB01593, Zinc DB14487, Zinc acetate DB14533, Zinc chloride DB14548, Zinc sulfate, unspecified form |
Genetic variation databases
BioMutai | S100A9 |
DMDMi | 115444 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000143997 | 1 – 114 | Protein S100-A9Add BLAST | 114 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | Blocked amino end (Thr) | 1 | |
Modified residuei | 3 | S-nitrosocysteine; transient1 Publication | 1 | |
Modified residuei | 105 | Pros-methylhistidineBy similarity | 1 | |
Modified residuei | 113 | Phosphothreonine; by MAPK14Combined sources3 Publications | 1 |
Post-translational modificationi
Keywords - PTMi
Methylation, Phosphoprotein, S-nitrosylationProteomic databases
CPTACi | CPTAC-1294 CPTAC-1295 non-CPTAC-1155 |
EPDi | P06702 |
jPOSTi | P06702 |
MassIVEi | P06702 |
MaxQBi | P06702 |
PaxDbi | P06702 |
PeptideAtlasi | P06702 |
PRIDEi | P06702 |
ProteomicsDBi | 51910 |
TopDownProteomicsi | P06702 |
2D gel databases
OGPi | P06702 |
SWISS-2DPAGEi | P06702 |
UCD-2DPAGEi | P06702 |
PTM databases
iPTMneti | P06702 |
MetOSitei | P06702 |
PhosphoSitePlusi | P06702 |
SwissPalmi | P06702 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000163220, Expressed in ectocervix and 199 other tissues |
Genevisiblei | P06702, HS |
Organism-specific databases
HPAi | ENSG00000163220, Tissue enhanced (blood, bone marrow, esophagus, tongue) |
Interactioni
Subunit structurei
Homodimer. Preferentially exists as a heterodimer or heterotetramer with S100A8 known as calprotectin (S100A8/A9). S100A9 interacts with ATP2A2 (By similarity). S100A9 interacts with AGER, and with the heterodimeric complex formed by TLR4 and LY96 in the presence of calcium and/or zinc ions. S100A9 binds quinoline-3-carboxamides in the presence of calcium and/or zinc ions. S100A9 interacts with amyloid-beta protein 40. Calprotectin (S100A8/9) interacts with CEACAM3 and tubulin filaments in a calcium-dependent manner. Heterotetrameric calprotectin (S100A8/A9) interacts with ANXA6 and associates with tubulin filaments in activated monocytes. Calprotectin (S100A8/9) interacts with NCF2/P67PHOX, RAC1, RAC2, CYBA and CYBB. Calprotectin (S100A8/9) interacts with NOS2 to form the iNOS-S100A8/A9 transnitrosylase complex; induced by LDL(ox) (PubMed:25417112).
By similarity1 Publication15 PublicationsBinary interactionsi
Hide detailsP06702
GO - Molecular functioni
- calcium-dependent protein binding Source: GO_Central
- microtubule binding Source: UniProtKB
- RAGE receptor binding Source: UniProtKB
- Toll-like receptor 4 binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 112188, 140 interactors |
ComplexPortali | CPX-37, Calprotectin heterotetramer CPX-39, Calprotectin heterodimer CPX-48, S100A9 complex CPX-52, iNOS-S100A8/A9 complex |
CORUMi | P06702 |
DIPi | DIP-1166N |
IntActi | P06702, 59 interactors |
MINTi | P06702 |
STRINGi | 9606.ENSP00000357727 |
Chemistry databases
BindingDBi | P06702 |
Miscellaneous databases
RNActi | P06702, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P06702 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06702 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 47 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 54 – 89 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502SA01, Eukaryota |
GeneTreei | ENSGT00940000161606 |
HOGENOMi | CLU_138624_6_0_1 |
InParanoidi | P06702 |
OMAi | NTFHHYS |
OrthoDBi | 1521098at2759 |
PhylomeDBi | P06702 |
TreeFami | TF332727 |
Family and domain databases
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR001751, S100/CaBP-9k_CS IPR013787, S100_Ca-bd_sub |
Pfami | View protein in Pfam PF01023, S_100, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 1 hit SM01394, S_100, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit PS00303, S100_CABP, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTCKMSQLER NIETIINTFH QYSVKLGHPD TLNQGEFKEL VRKDLQNFLK
60 70 80 90 100
KENKNEKVIE HIMEDLDTNA DKQLSFEEFI MLMARLTWAS HEKMHEGDEG
110
PGHHHKPGLG EGTP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | S → H AA sequence (PubMed:8423249).Curated | 1 | |
Sequence conflicti | 25 | K → F AA sequence (PubMed:8423249).Curated | 1 | |
Sequence conflicti | 28 | H → L AA sequence (PubMed:8423249).Curated | 1 |
Mass spectrometryi
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_013008 | 20 | H → R1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06233 mRNA Translation: CAA29579.1 M21064 Genomic DNA Translation: AAA36326.1 M26311 mRNA Translation: AAA68480.1 AF237581 Genomic DNA Translation: AAF62536.1 AF237582 Genomic DNA Translation: AAF62537.1 CR542207 mRNA Translation: CAG47003.1 CR542224 mRNA Translation: CAG47020.1 AL591704 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW53333.1 CH471121 Genomic DNA Translation: EAW53334.1 BC047681 mRNA Translation: AAH47681.1 |
CCDSi | CCDS1036.1 |
PIRi | B31848 |
RefSeqi | NP_002956.1, NM_002965.3 |
Genome annotation databases
Ensembli | ENST00000368738; ENSP00000357727; ENSG00000163220 |
GeneIDi | 6280 |
KEGGi | hsa:6280 |
UCSCi | uc001fbq.3, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06233 mRNA Translation: CAA29579.1 M21064 Genomic DNA Translation: AAA36326.1 M26311 mRNA Translation: AAA68480.1 AF237581 Genomic DNA Translation: AAF62536.1 AF237582 Genomic DNA Translation: AAF62537.1 CR542207 mRNA Translation: CAG47003.1 CR542224 mRNA Translation: CAG47020.1 AL591704 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW53333.1 CH471121 Genomic DNA Translation: EAW53334.1 BC047681 mRNA Translation: AAH47681.1 |
CCDSi | CCDS1036.1 |
PIRi | B31848 |
RefSeqi | NP_002956.1, NM_002965.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IRJ | X-ray | 2.10 | A/B/C/D/E/F/G/H | 2-114 | [»] | |
1XK4 | X-ray | 1.80 | C/D/G/H/K/L | 2-114 | [»] | |
4GGF | X-ray | 1.60 | C/L/T/V | 1-114 | [»] | |
4XJK | X-ray | 1.76 | B/D/F/H/J | 1-114 | [»] | |
5I8N | NMR | - | A/B | 1-114 | [»] | |
5W1F | X-ray | 2.60 | B/D/F/H | 1-114 | [»] | |
6DS2 | X-ray | 2.10 | B/D/F/H | 1-114 | [»] | |
SMRi | P06702 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112188, 140 interactors |
ComplexPortali | CPX-37, Calprotectin heterotetramer CPX-39, Calprotectin heterodimer CPX-48, S100A9 complex CPX-52, iNOS-S100A8/A9 complex |
CORUMi | P06702 |
DIPi | DIP-1166N |
IntActi | P06702, 59 interactors |
MINTi | P06702 |
STRINGi | 9606.ENSP00000357727 |
Chemistry databases
BindingDBi | P06702 |
DrugBanki | DB01373, Calcium DB01593, Zinc DB14487, Zinc acetate DB14533, Zinc chloride DB14548, Zinc sulfate, unspecified form |
PTM databases
iPTMneti | P06702 |
MetOSitei | P06702 |
PhosphoSitePlusi | P06702 |
SwissPalmi | P06702 |
Genetic variation databases
BioMutai | S100A9 |
DMDMi | 115444 |
2D gel databases
OGPi | P06702 |
SWISS-2DPAGEi | P06702 |
UCD-2DPAGEi | P06702 |
Proteomic databases
CPTACi | CPTAC-1294 CPTAC-1295 non-CPTAC-1155 |
EPDi | P06702 |
jPOSTi | P06702 |
MassIVEi | P06702 |
MaxQBi | P06702 |
PaxDbi | P06702 |
PeptideAtlasi | P06702 |
PRIDEi | P06702 |
ProteomicsDBi | 51910 |
TopDownProteomicsi | P06702 |
Protocols and materials databases
Antibodypediai | 1084, 1165 antibodies |
CPTCi | P06702, 2 antibodies |
Genome annotation databases
Ensembli | ENST00000368738; ENSP00000357727; ENSG00000163220 |
GeneIDi | 6280 |
KEGGi | hsa:6280 |
UCSCi | uc001fbq.3, human |
Organism-specific databases
CTDi | 6280 |
DisGeNETi | 6280 |
GeneCardsi | S100A9 |
HGNCi | HGNC:10499, S100A9 |
HPAi | ENSG00000163220, Tissue enhanced (blood, bone marrow, esophagus, tongue) |
MIMi | 123886, gene |
neXtProti | NX_P06702 |
OpenTargetsi | ENSG00000163220 |
PharmGKBi | PA34911 |
VEuPathDBi | HostDB:ENSG00000163220.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502SA01, Eukaryota |
GeneTreei | ENSGT00940000161606 |
HOGENOMi | CLU_138624_6_0_1 |
InParanoidi | P06702 |
OMAi | NTFHHYS |
OrthoDBi | 1521098at2759 |
PhylomeDBi | P06702 |
TreeFami | TF332727 |
Enzyme and pathway databases
PathwayCommonsi | P06702 |
Reactomei | R-HSA-5668599, RHO GTPases Activate NADPH Oxidases R-HSA-5686938, Regulation of TLR by endogenous ligand R-HSA-6798695, Neutrophil degranulation R-HSA-6799990, Metal sequestration by antimicrobial proteins |
SIGNORi | P06702 |
Miscellaneous databases
BioGRID-ORCSi | 6280, 7 hits in 876 CRISPR screens |
ChiTaRSi | S100A9, human |
EvolutionaryTracei | P06702 |
GeneWikii | S100A9 |
GenomeRNAii | 6280 |
Pharosi | P06702, Tbio |
PROi | PR:P06702 |
RNActi | P06702, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163220, Expressed in ectocervix and 199 other tissues |
Genevisiblei | P06702, HS |
Family and domain databases
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR001751, S100/CaBP-9k_CS IPR013787, S100_Ca-bd_sub |
Pfami | View protein in Pfam PF01023, S_100, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 1 hit SM01394, S_100, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit PS00303, S100_CABP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | S10A9_HUMAN | |
Accessioni | P06702Primary (citable) accession number: P06702 Secondary accession number(s): D3DV36 Q9UCJ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | February 10, 2021 | |
This is version 228 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families