UniProtKB - P06687 (AT1A3_RAT)
Sodium/potassium-transporting ATPase subunit alpha-3
Atp1a3
Functioni
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Catalytic activityi
- EC:7.2.2.13
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 366 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 707 | MagnesiumBy similarity | 1 | |
Metal bindingi | 711 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- amyloid-beta binding Source: ARUK-UCL
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- chaperone binding Source: RGD
- D1 dopamine receptor binding Source: RGD
- heparan sulfate proteoglycan binding Source: RGD
- ion antiporter activity involved in regulation of presynaptic membrane potential Source: RGD
- metal ion binding Source: UniProtKB-KW
- P-type sodium:potassium-exchanging transporter activity Source: UniProtKB
- P-type sodium:potassium-exchanging transporter activity involved in regulation of cardiac muscle cell membrane potential Source: RGD
GO - Biological processi
- adult locomotory behavior Source: RGD
- cardiac muscle contraction Source: RGD
- cellular potassium ion homeostasis Source: RGD
- cellular response to amyloid-beta Source: ARUK-UCL
- cellular response to retinoic acid Source: RGD
- cellular response to thyroid hormone stimulus Source: RGD
- cellular sodium ion homeostasis Source: RGD
- cerebral cortex development Source: RGD
- ionotropic glutamate receptor signaling pathway Source: RGD
- memory Source: RGD
- neuron projection maintenance Source: RGD
- potassium ion import across plasma membrane Source: RGD
- potassium ion transport Source: RGD
- proton transmembrane transport Source: GO_Central
- regulation of cardiac muscle cell membrane potential Source: RGD
- response to xenobiotic stimulus Source: RGD
- sodium ion export across plasma membrane Source: RGD
- sodium ion transport Source: RGD
- visual learning Source: RGD
Keywordsi
Molecular function | Translocase |
Biological process | Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium |
Enzyme and pathway databases
Reactomei | R-RNO-5578775, Ion homeostasis R-RNO-936837, Ion transport by P-type ATPases |
SABIO-RKi | P06687 |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium/potassium-transporting ATPase subunit alpha-3 (EC:7.2.2.13)Short name: Na(+)/K(+) ATPase alpha-3 subunit Alternative name(s): Na(+)/K(+) ATPase alpha(III) subunit Sodium pump subunit alpha-3 |
Gene namesi | Name:Atp1a3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2169, Atp1a3 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: UniProtKB
Nucleus
- nucleus Source: RGD
Plasma Membrane
- integral component of presynaptic membrane Source: SynGO
- plasma membrane Source: UniProtKB
- sarcolemma Source: RGD
- sodium:potassium-exchanging ATPase complex Source: RGD
Other locations
- axon Source: RGD
- calyx of Held Source: SynGO
- cytoplasm Source: RGD
- dendritic spine head Source: RGD
- dendritic spine neck Source: RGD
- membrane Source: ARUK-UCL
- myelin sheath Source: UniProtKB
- neuron to neuron synapse Source: RGD
- neuronal cell body Source: RGD
- organelle membrane Source: RGD
- photoreceptor inner segment Source: RGD
- postsynapse Source: SynGO
- synapse Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 77 | CytoplasmicSequence analysisAdd BLAST | 77 | |
Transmembranei | 78 – 98 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 99 – 121 | ExtracellularSequence analysisAdd BLAST | 23 | |
Transmembranei | 122 – 142 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 143 – 278 | CytoplasmicSequence analysisAdd BLAST | 136 | |
Transmembranei | 279 – 298 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 299 – 310 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 311 – 328 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 329 – 762 | CytoplasmicSequence analysisAdd BLAST | 434 | |
Transmembranei | 763 – 782 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 783 – 792 | ExtracellularSequence analysis | 10 | |
Transmembranei | 793 – 813 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 814 – 833 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 834 – 856 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 857 – 908 | ExtracellularSequence analysisAdd BLAST | 52 | |
Transmembranei | 909 – 928 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 929 – 941 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 942 – 960 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 961 – 975 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 976 – 996 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 997 – 1013 | CytoplasmicSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046300 | 1 – 1013 | Sodium/potassium-transporting ATPase subunit alpha-3Add BLAST | 1013 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 37 | PhosphoserineCombined sources | 1 | |
Modified residuei | 56 | PhosphoserineBy similarity | 1 | |
Modified residuei | 218 | PhosphoserineCombined sources | 1 | |
Modified residuei | 265 | PhosphoserineBy similarity | 1 | |
Modified residuei | 442 | PhosphoserineCombined sources | 1 | |
Modified residuei | 548 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 933 | Phosphoserine; by PKABy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P06687 |
PaxDbi | P06687 |
PRIDEi | P06687 |
PTM databases
CarbonylDBi | P06687 |
iPTMneti | P06687 |
PhosphoSitePlusi | P06687 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000020263, Expressed in brain and 22 other tissues |
Genevisiblei | P06687, RN |
Interactioni
Subunit structurei
The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.
Interacts with regulatory subunit FXYD1.
1 PublicationGO - Molecular functioni
- chaperone binding Source: RGD
- D1 dopamine receptor binding Source: RGD
- heparan sulfate proteoglycan binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 246401, 9 interactors |
IntActi | P06687, 4 interactors |
MINTi | P06687 |
STRINGi | 10116.ENSRNOP00000027497 |
Chemistry databases
BindingDBi | P06687 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 24 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 72 – 74 | Interaction with phosphoinositide-3 kinaseBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0203, Eukaryota |
GeneTreei | ENSGT00940000160476 |
HOGENOMi | CLU_002360_4_3_1 |
InParanoidi | P06687 |
OMAi | RRFLTYH |
OrthoDBi | 388324at2759 |
Family and domain databases
CDDi | cd02608, P-type_ATPase_Na-K_like, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005775, P-type_ATPase_IIC IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01106, ATPase-IIC_X-K, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MGDKKDDKSS PKKSKAKERR DLDDLKKEVA MTEHKMSVEE VCRKYNTDCV
60 70 80 90 100
QGLTHSKAQE ILARDGPNAL TPPPTTPEWV KFCRQLFGGF SILLWIGAIL
110 120 130 140 150
CFLAYGIQAG TEDDPSGDNL YLGIVLAAVV IITGCFSYYQ EAKSSKIMES
160 170 180 190 200
FKNMVPQQAL VIREGEKMQV NAEEVVVGDL VEIKGGDRVP ADLRIISAHG
210 220 230 240 250
CKVDNSSLTG ESEPQTRSPD CTHDNPLETR NITFFSTNCV EGTARGVVVA
260 270 280 290 300
TGDRTVMGRI ATLASGLEVG KTPIAIEIEH FIQLITGVAV FLGVSFFILS
310 320 330 340 350
LILGYTWLEA VIFLIGIIVA NVPEGLLATV TVCLTLTAKR MARKNCLVKN
360 370 380 390 400
LEAVETLGST STICSDKTGT LTQNRMTVAH MWFDNQIHEA DTTEDQSGTS
410 420 430 440 450
FDKSSHTWVA LSHIAGLCNR AVFKGGQDNI PVLKRDVAGD ASESALLKCI
460 470 480 490 500
ELSSGSVKLM RERNKKVAEI PFNSTNKYQL SIHETEDPND NRYLLVMKGA
510 520 530 540 550
PERILDRCAT ILLQGKEQPL DEEMKEAFQN AYLELGGLGE RVLGFCHYYL
560 570 580 590 600
PEEQFPKGFA FDCDDVNFTT DNLCFVGLMS MIDPPRAAVP DAVGKCRSAG
610 620 630 640 650
IKVIMVTGDH PITAKAIAKG VGIISEGNET VEDIAARLNI PVSQVNPRDA
660 670 680 690 700
KACVIHGTDL KDFTSEQIDE ILQNHTEIVF ARTSPQQKLI IVEGCQRQGA
710 720 730 740 750
IVAVTGDGVN DSPALKKADI GVAMGIAGSD VSKQAADMIL LDDNFASIVT
760 770 780 790 800
GVEEGRLIFD NLKKSIAYTL TSNIPEITPF LLFIMANIPL PLGTITILCI
810 820 830 840 850
DLGTDMVPAI SLAYEAAESD IMKRQPRNPR TDKLVNERLI SMAYGQIGMI
860 870 880 890 900
QALGGFFSYF VILAENGFLP GNLVGIRLNW DDRTVNDLED SYGQQWTYEQ
910 920 930 940 950
RKVVEFTCHT AFFVSIVVVQ WADLIICKTR RNSVFQQGMK NKILIFGLFE
960 970 980 990 1000
ETALAAFLSY CPGMDVALRM YPLKPSWWFC AFPYSFLIFV YDEIRKLILR
1010
RNPGGWVEKE TYY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A8I5ZQ22 | A0A8I5ZQ22_RAT | Sodium/potassium-transporting ATPas... | Atp1a3 | 1,010 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 3 | MGD → MNL in AAA41672 (PubMed:2822726).Curated | 3 | |
Sequence conflicti | 104 | A → R in AAA41672 (PubMed:2822726).Curated | 1 | |
Sequence conflicti | 114 | D → E in AAA41672 (PubMed:2822726).Curated | 1 | |
Sequence conflicti | 128 | A → G in AAA41672 (PubMed:2822726).Curated | 1 | |
Sequence conflicti | 149 | E → Q in AAA41672 (PubMed:2822726).Curated | 1 | |
Sequence conflicti | 152 | K → T in AAA41672 (PubMed:2822726).Curated | 1 | |
Sequence conflicti | 166 | E → D in AAA41672 (PubMed:2822726).Curated | 1 | |
Sequence conflicti | 192 – 194 | DLR → ELG in AAA41672 (PubMed:2822726).Curated | 3 | |
Sequence conflicti | 200 | G → R in AAA41672 (PubMed:2822726).Curated | 1 | |
Sequence conflicti | 339 | Missing in AAA41672 (PubMed:2822726).Curated | 1 | |
Sequence conflicti | 621 | V → K in AAA41672 (PubMed:2822726).Curated | 1 | |
Sequence conflicti | 807 – 809 | VPA → DPT in AAA41672 (PubMed:2822726).Curated | 3 | |
Sequence conflicti | 908 | C → F in AAA40777 (PubMed:3028470).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14513 mRNA Translation: AAA40777.1 X05883 mRNA Translation: CAA29307.1 M28648 mRNA Translation: AAA41672.1 |
PIRi | C24639 |
RefSeqi | NP_036638.1, NM_012506.1 |
Genome annotation databases
GeneIDi | 24213 |
KEGGi | rno:24213 |
UCSCi | RGD:2169, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14513 mRNA Translation: AAA40777.1 X05883 mRNA Translation: CAA29307.1 M28648 mRNA Translation: AAA41672.1 |
PIRi | C24639 |
RefSeqi | NP_036638.1, NM_012506.1 |
3D structure databases
AlphaFoldDBi | P06687 |
SMRi | P06687 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 246401, 9 interactors |
IntActi | P06687, 4 interactors |
MINTi | P06687 |
STRINGi | 10116.ENSRNOP00000027497 |
Chemistry databases
BindingDBi | P06687 |
ChEMBLi | CHEMBL3885640 CHEMBL4106149 |
PTM databases
CarbonylDBi | P06687 |
iPTMneti | P06687 |
PhosphoSitePlusi | P06687 |
Proteomic databases
jPOSTi | P06687 |
PaxDbi | P06687 |
PRIDEi | P06687 |
Genome annotation databases
GeneIDi | 24213 |
KEGGi | rno:24213 |
UCSCi | RGD:2169, rat |
Organism-specific databases
CTDi | 478 |
RGDi | 2169, Atp1a3 |
Phylogenomic databases
eggNOGi | KOG0203, Eukaryota |
GeneTreei | ENSGT00940000160476 |
HOGENOMi | CLU_002360_4_3_1 |
InParanoidi | P06687 |
OMAi | RRFLTYH |
OrthoDBi | 388324at2759 |
Enzyme and pathway databases
Reactomei | R-RNO-5578775, Ion homeostasis R-RNO-936837, Ion transport by P-type ATPases |
SABIO-RKi | P06687 |
Miscellaneous databases
PROi | PR:P06687 |
Gene expression databases
Bgeei | ENSRNOG00000020263, Expressed in brain and 22 other tissues |
Genevisiblei | P06687, RN |
Family and domain databases
CDDi | cd02608, P-type_ATPase_Na-K_like, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005775, P-type_ATPase_IIC IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01106, ATPase-IIC_X-K, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AT1A3_RAT | |
Accessioni | P06687Primary (citable) accession number: P06687 Secondary accession number(s): Q16732, Q9Z1G6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | October 18, 2001 | |
Last modified: | May 25, 2022 | |
This is version 202 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families