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Protein

Photosystem II protein D1

Gene

psbA

Organism
Euglena gracilis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Photosystem II (PSII) is a light-driven water: plastoquinone oxidoreductase that uses light energy to abstract electrons from H2O, generating O2 and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1/D2 (PsbA/PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors.UniRule annotation

Miscellaneous

2 of the reaction center chlorophylls (ChlD1 and ChlD2) are entirely coordinated by water.UniRule annotation
Herbicides such as atrazine, BNT, diuron or ioxynil bind in the Q(B) binding site and block subsequent electron transfer.UniRule annotation

Catalytic activityi

2 H2O + 2 plastoquinone + 4 light = O2 + 2 plastoquinol.UniRule annotation

Cofactori

Note: The D1/D2 heterodimer binds P680, chlorophylls that are the primary electron donor of PSII, and subsequent electron acceptors. It shares a non-heme iron and each subunit binds pheophytin, quinone, additional chlorophylls, carotenoids and lipids. D1 provides most of the ligands for the Mn4-Ca-O5 cluster of the oxygen-evolving complex (OEC). There is also a Cl(-1) ion associated with D1 and D2, which is required for oxygen evolution. The PSII complex binds additional chlorophylls, carotenoids and specific lipids.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi119Magnesium (chlorophyll-a ChlzD1 axial ligand); via tele nitrogenUniRule annotation1
Binding sitei127Pheophytin D1UniRule annotation1
Sitei162Tyrosine radical intermediateUniRule annotation1
Metal bindingi171Calcium-manganese-oxide [Ca-4Mn-5O]; calciumUniRule annotation1
Metal bindingi171Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 4UniRule annotation1
Metal bindingi190Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1UniRule annotation1
Sitei191Stabilizes free radical intermediateUniRule annotation1
Metal bindingi199Magnesium (chlorophyll-a PD1 axial ligand); via tele nitrogenUniRule annotation1
Metal bindingi216Iron; shared with heterodimeric partner; via tele nitrogenUniRule annotation1
Binding sitei216Quinone (B)UniRule annotation1
Metal bindingi273Iron; shared with heterodimeric partner; via tele nitrogenUniRule annotation1
Metal bindingi333Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1; via tele nitrogenUniRule annotation1
Metal bindingi334Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 3UniRule annotation1
Metal bindingi334Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 4UniRule annotation1
Metal bindingi343Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1UniRule annotation1
Metal bindingi343Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 2UniRule annotation1
Metal bindingi345Calcium-manganese-oxide [Ca-4Mn-5O]; calcium; via carboxylateUniRule annotation1
Metal bindingi345Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 2; via carboxylateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Herbicide resistance, Photosynthesis, Transport
LigandCalcium, Chlorophyll, Chromophore, Iron, Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem II protein D1UniRule annotation (EC:1.10.3.9UniRule annotation)
Short name:
PSII D1 proteinUniRule annotation
Alternative name(s):
32 kDa thylakoid membrane protein1 Publication
Photosystem II Q(B) proteinUniRule annotation
Gene namesi
Name:psbAUniRule annotation
Encoded oniPlastid; Chloroplast
OrganismiEuglena gracilis
Taxonomic identifieri3039 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaEuglenidaEuglenalesEuglenaceaeEuglena

Subcellular locationi

  • chloroplast thylakoid membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei30 – 47HelicalUniRule annotationAdd BLAST18
Transmembranei119 – 134HelicalUniRule annotationAdd BLAST16
Transmembranei143 – 157HelicalUniRule annotationAdd BLAST15
Transmembranei198 – 219HelicalUniRule annotationAdd BLAST22
Transmembranei275 – 289HelicalUniRule annotationAdd BLAST15

GO - Cellular componenti

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem II, Plastid, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi265S → A: Herbicide resistance. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000904401 – 345Photosystem II protein D1UniRule annotationAdd BLAST345

Post-translational modificationi

Tyr-162 forms a radical intermediate that is referred to as redox-active TyrZ, YZ or Y-Z.UniRule annotation

Proteomic databases

PRIDEiP06631

Interactioni

Subunit structurei

PSII is composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral proteins of the oxygen-evolving complex and a large number of cofactors. It forms dimeric complexes.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP06631
SMRiP06631
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 266Quinone (B)UniRule annotation2

Sequence similaritiesi

Belongs to the reaction center PufL/M/PsbA/D family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

CDDicd09289 Photosystem-II_D1, 1 hit
Gene3Di1.20.85.10, 1 hit
HAMAPiMF_01379 PSII_PsbA_D1, 1 hit
InterProiView protein in InterPro
IPR036854 Photo_II_D1/D2_sf
IPR000484 Photo_RC_L/M
IPR005867 PSII_D1
PANTHERiPTHR33149 PTHR33149, 1 hit
PfamiView protein in Pfam
PF00124 Photo_RC, 1 hit
PRINTSiPR00256 REACTNCENTRE
SUPFAMiSSF81483 SSF81483, 1 hit
TIGRFAMsiTIGR01151 psbA, 1 hit
PROSITEiView protein in PROSITE
PS00244 REACTION_CENTER, 1 hit

Sequencei

Sequence statusi: Complete.

P06631-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISPVLKKYA RPSLWYRFCA WVASKKNRLY VGWFGVLMIP TLLTAATVFI
60 70 80 90 100
IAFIAAPPVD IDGIREPVSG SLFYGNNIIT GAVVPTSNAI GLHFYPIWEA
110 120 130 140 150
TSLDEWLYNG GPYQLIVCHF FIGICSYMGR EWELSFRLGM RPWIAVAYSA
160 170 180 190 200
PVAAASAVFI VYPLGQGSFS DGMPLGISGT FNFMIVFQAE HNILMHPFHM
210 220 230 240 250
LGVAGVFGGS LFSAMHGSLV TSSLLRETTE NESINVGYKF GQEEETYNII
260 270 280 290 300
AAHAYFGRLI FQYASFNNSR SLHFFLAVWP VVGIWFTALG VSTMAFNLNG
310 320 330 340
FNFNQSVIDS QGRVINTWAD IINRANLGME VMHERNAHNF PLDLA
Length:345
Mass (Da):38,365
Last modified:February 1, 1994 - v2
Checksum:iC2F3B5435EAFFDAD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79I → L in CAA25447 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11874 Genomic DNA No translation available.
X00929 Genomic DNA Translation: CAA25447.1
X00735 Genomic DNA Translation: CAA25319.1
X70810 Genomic DNA Translation: CAA50082.1
PIRiA22883 S34503
RefSeqiNP_041895.1, NC_001603.2

Genome annotation databases

GeneIDi807514

Similar proteinsi

Entry informationi

Entry nameiPSBA_EUGGR
AccessioniPrimary (citable) accession number: P06631
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: February 1, 1994
Last modified: February 28, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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