UniProtKB - P06616 (ERA_ECOLI)
GTPase Era
era
Functioni
An essential GTPase that binds both GDP and GTP, with nucleotide exchange occurring on the order of seconds whereas hydrolysis occurs on the order of minutes. Plays a role in numerous processes, including cell cycle regulation, energy metabolism, as a chaperone for 16S rRNA processing and 30S ribosomal subunit biogenesis. One of at least 4 proteins (Era, RbfA, RimM and RsgA/YjeQ) that assist in the late assembly stage of the 30S ribosomal subunit. Its presence in the 30S subunit may prevent translation initiation. Seems to be critical for maintaining cell growth and cell divison rates; a dramatic reduction in Era protein levels temporarily arrests cell growth just before cytokinesis (at the predivisional two-cell stage) and delays cell division. Era mutant era1 suppresses some temperature-sensitive mutations that affect DNA replication and chromosome partitioning and segregation. The dominant-negative Era-de mutant which is missing residues in a putative effector region, is unable to complement the disruption mutant; upon overproduction it shows a significant decrease in cell viability and a synthetic lethal phenotype in the presence of acetate. Era function probably overlaps RbfA (PubMed:16825789).
Binds to the pre-30S ribosomal subunit through several stages of protein assembly (PubMed:20188109).
4 PublicationsMiscellaneous
Activity regulationi
Kineticsi
- KM=15.4 µM for GTP (for His-tagged protein at pH 8.0, 5 mM MgCl2)2 Publications
- KM=9.0 µM for GTP (for overexpressed protein at pH 8.0, 5 mM MgCl2)2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 15 – 22 | GTP | 8 | |
Nucleotide bindingi | 62 – 66 | GTP | 5 | |
Nucleotide bindingi | 124 – 127 | GTP | 4 |
GO - Molecular functioni
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB
- guanosine tetraphosphate binding Source: EcoCyc
- ribosomal small subunit binding Source: UniProtKB
- RNA binding Source: EcoCyc
- rRNA binding Source: GO_Central
- small ribosomal subunit rRNA binding Source: UniProtKB
GO - Biological processi
- protein autophosphorylation Source: EcoCyc
- protein phosphorylation Source: UniProtKB
- ribosomal small subunit assembly Source: GO_Central
- ribosomal small subunit biogenesis Source: EcoCyc
Keywordsi
Molecular function | RNA-binding, rRNA-binding |
Biological process | Ribosome biogenesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10270-MONOMER |
SABIO-RKi | P06616 |
Names & Taxonomyi
Protein namesi | Recommended name: GTPase EraShort name: ERA Alternative name(s): GTP-binding protein Era |
Gene namesi | Name:era Synonyms:rbaA, sdgE Ordered Locus Names:b2566, JW2550 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Plasma membrane
- Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Note: Binding is GDP or GTP-dependent, slightly more protein is bound in the presence of GTP than GDP.
Cytosol
- cytosol Source: EcoCyc
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: EcoCyc
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, Cytoplasm, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 8 | C → A in era770; 20-fold reduction in GTP-binding. Confers growth sensitivity at 42 degrees Celsius; when associated with an unpublished 22 residue replacement in the C-terminus. 1 Publication | 1 | |
Mutagenesisi | 17 | P → R in era1; suppresses a number of temperature-sensitive mutations affecting cell cycle. 2 Publications | 1 | |
Mutagenesisi | 17 | P → V: Confers sensitivity to cold. 2 Publications | 1 | |
Mutagenesisi | 26 | N → S: Confers sensitivity to cold; overexpression does not suppress an rbfA disruption. 1 Publication | 1 | |
Mutagenesisi | 39 – 49 | KAQTTRHRIVG → R in Era-de; does not complement a disruption mutant, overexpression in a wt cells inhibits growth. Binds GTP poorly, Km for GTP increases 5-fold, Vmax for GTPase is 55% that of wt. Does not autophosphorylate. Complements cold-sensitivity and 16S rRNA processing in an rbfA disruption mutant. 1 PublicationAdd BLAST | 11 | |
Mutagenesisi | 42 – 43 | TT → AA: Does not complement a disruption mutant, Km for GTP increases 12-fold, Vmax for GTPase is 49% that of wt. Overexpression partially suppresses an rbfA disruption. 2 Publications | 2 | |
Mutagenesisi | 156 | A → D: Confers sensitivity to cold; overexpression partially suppresses an rbfA disruption. 1 Publication | 1 | |
Mutagenesisi | 200 | E → K: Confers sensitivity to cold, cells do not divide properly but do replicate DNA and segregate nucleoids normally. 16S rRNA processing is decreased, ribosome assembly is defective. Suppressed by overexpression of RsmA. Overexpression does not suppress an rbfA disruption, while at 37 degrees Celsius the rbfA/era E220K mutant grows very poorly, a dominant-negative effect. This mutant still binds 30S ribosomes. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000180012 | 2 – 301 | GTPase EraAdd BLAST | 300 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 36 | Phosphothreonine; by autocatalysis1 Publication | 1 | |
Modified residuei | 37 | Phosphoserine; by autocatalysis1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P06616 |
PaxDbi | P06616 |
PRIDEi | P06616 |
PTM databases
iPTMneti | P06616 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Monomer. Binds both 16S rRNA and 30S ribosomal subunits; binding is inhibited by GDP and GTP (PubMed:10094501). Bind preferentially to mature 30S ribosomal subunits over immature subunits in the presence of GMP-PNP (PubMed:27382067). Binds to MazG; GDP-bound Era binds more tightly to MazG than GTP-bound Era (PubMed:19706445).
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4263220, 465 interactors 851375, 7 interactors |
DIPi | DIP-9521N |
IntActi | P06616, 29 interactors |
STRINGi | 511145.b2566 |
Structurei
Secondary structure
3D structure databases
SMRi | P06616 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06616 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 175 | Era-type GPROSITE-ProRule annotationAdd BLAST | 169 | |
Domaini | 206 – 283 | KH type-2Add BLAST | 78 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 15 – 22 | G1PROSITE-ProRule annotation | 8 | |
Regioni | 41 – 45 | G2PROSITE-ProRule annotation | 5 | |
Regioni | 62 – 65 | G3PROSITE-ProRule annotation | 4 | |
Regioni | 124 – 127 | G4PROSITE-ProRule annotation | 4 | |
Regioni | 154 – 156 | G5PROSITE-ProRule annotation | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1159, Bacteria |
HOGENOMi | CLU_038009_1_2_6 |
InParanoidi | P06616 |
PhylomeDBi | P06616 |
Family and domain databases
CDDi | cd04163, Era, 1 hit |
Gene3Di | 3.30.300.20, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_00367, GTPase_Era, 1 hit |
InterProi | View protein in InterPro IPR030388, G_ERA_dom IPR006073, GTP-bd IPR005662, GTP-bd_Era IPR015946, KH_dom-like_a/b IPR004044, KH_dom_type_2 IPR009019, KH_sf_prok-type IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom |
PANTHERi | PTHR42698, PTHR42698, 1 hit |
Pfami | View protein in Pfam PF07650, KH_2, 1 hit PF01926, MMR_HSR1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF54814, SSF54814, 1 hit |
TIGRFAMsi | TIGR00436, era, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51713, G_ERA, 1 hit PS50823, KH_TYPE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSIDKSYCGF IAIVGRPNVG KSTLLNKLLG QKISITSRKA QTTRHRIVGI
60 70 80 90 100
HTEGAYQAIY VDTPGLHMEE KRAINRLMNK AASSSIGDVE LVIFVVEGTR
110 120 130 140 150
WTPDDEMVLN KLREGKAPVI LAVNKVDNVQ EKADLLPHLQ FLASQMNFLD
160 170 180 190 200
IVPISAETGL NVDTIAAIVR KHLPEATHHF PEDYITDRSQ RFMASEIIRE
210 220 230 240 250
KLMRFLGAEL PYSVTVEIER FVSNERGGYD INGLILVERE GQKKMVIGNK
260 270 280 290 300
GAKIKTIGIE ARKDMQEMFE APVHLELWVK VKSGWADDER ALRSLGYVDD
L
Mass spectrometryi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14658 Unassigned DNA Translation: AAA03242.1 D64044 Genomic DNA Translation: BAA10913.1 U36841 Genomic DNA Translation: AAA79828.1 U00096 Genomic DNA Translation: AAC75619.1 AP009048 Genomic DNA Translation: BAE76742.1 X02673 Genomic DNA Translation: CAA26505.1 |
PIRi | S44713, RGECGT |
RefSeqi | NP_417061.1, NC_000913.3 WP_000020749.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75619; AAC75619; b2566 BAE76742; BAE76742; BAE76742 |
GeneIDi | 58391288 947036 |
KEGGi | ecj:JW2550 eco:b2566 |
PATRICi | fig|1411691.4.peg.4168 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14658 Unassigned DNA Translation: AAA03242.1 D64044 Genomic DNA Translation: BAA10913.1 U36841 Genomic DNA Translation: AAA79828.1 U00096 Genomic DNA Translation: AAC75619.1 AP009048 Genomic DNA Translation: BAE76742.1 X02673 Genomic DNA Translation: CAA26505.1 |
PIRi | S44713, RGECGT |
RefSeqi | NP_417061.1, NC_000913.3 WP_000020749.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EGA | X-ray | 2.40 | A/B | 1-301 | [»] | |
3IEU | X-ray | 2.80 | A/B | 1-301 | [»] | |
SMRi | P06616 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263220, 465 interactors 851375, 7 interactors |
DIPi | DIP-9521N |
IntActi | P06616, 29 interactors |
STRINGi | 511145.b2566 |
PTM databases
iPTMneti | P06616 |
Proteomic databases
jPOSTi | P06616 |
PaxDbi | P06616 |
PRIDEi | P06616 |
Genome annotation databases
EnsemblBacteriai | AAC75619; AAC75619; b2566 BAE76742; BAE76742; BAE76742 |
GeneIDi | 58391288 947036 |
KEGGi | ecj:JW2550 eco:b2566 |
PATRICi | fig|1411691.4.peg.4168 |
Organism-specific databases
EchoBASEi | EB0266 |
Phylogenomic databases
eggNOGi | COG1159, Bacteria |
HOGENOMi | CLU_038009_1_2_6 |
InParanoidi | P06616 |
PhylomeDBi | P06616 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10270-MONOMER |
SABIO-RKi | P06616 |
Miscellaneous databases
EvolutionaryTracei | P06616 |
PROi | PR:P06616 |
Family and domain databases
CDDi | cd04163, Era, 1 hit |
Gene3Di | 3.30.300.20, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_00367, GTPase_Era, 1 hit |
InterProi | View protein in InterPro IPR030388, G_ERA_dom IPR006073, GTP-bd IPR005662, GTP-bd_Era IPR015946, KH_dom-like_a/b IPR004044, KH_dom_type_2 IPR009019, KH_sf_prok-type IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom |
PANTHERi | PTHR42698, PTHR42698, 1 hit |
Pfami | View protein in Pfam PF07650, KH_2, 1 hit PF01926, MMR_HSR1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF54814, SSF54814, 1 hit |
TIGRFAMsi | TIGR00436, era, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51713, G_ERA, 1 hit PS50823, KH_TYPE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ERA_ECOLI | |
Accessioni | P06616Primary (citable) accession number: P06616 Secondary accession number(s): Q2MAG4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 202 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families