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UniProtKB - P06611 (BTUD_ECOLI)
Protein
Vitamin B12 import ATP-binding protein BtuD
Gene
btuD
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Responsible for energy coupling to the transport system.
UniRule annotationCatalytic activityi
- an R-cob(III)alamin(out) + ATP + H2O = ADP + an R-cob(III)alamin(in) + H+ + phosphateUniRule annotationEC:7.6.2.8UniRule annotation
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 33 – 40 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ABC-type vitamin B12 transporter activity Source: EcoCyc
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: EcoCyc
- ATP hydrolysis activity Source: InterPro
GO - Biological processi
- cobalamin transport Source: EcoCyc
Keywordsi
Molecular function | Translocase |
Biological process | Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:BTUD-MONOMER |
BRENDAi | 7.6.2.8, 2026 |
Protein family/group databases
TCDBi | 3.A.1.13.1, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Vitamin B12 import ATP-binding protein BtuDUniRule annotation (EC:7.6.2.8UniRule annotation)Alternative name(s): Vitamin B12-transporting ATPaseUniRule annotation |
Gene namesi | Name:btuDUniRule annotation Ordered Locus Names:b1709, JW1699 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- ATP-binding cassette (ABC) transporter complex Source: EcoCyc
- cobalamin transport complex Source: ComplexPortal
Other locations
- extrinsic component of membrane Source: EcoliWiki
- membrane Source: ComplexPortal
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000091950 | 1 – 249 | Vitamin B12 import ATP-binding protein BtuDAdd BLAST | 249 |
Proteomic databases
jPOSTi | P06611 |
PaxDbi | P06611 |
PRIDEi | P06611 |
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (BtuD), two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).
UniRule annotation1 PublicationBinary interactionsi
P06611
With | #Exp. | IntAct |
---|---|---|
btuC [P06609] | 13 | EBI-1033420,EBI-1033427 |
Protein-protein interaction databases
BioGRIDi | 4260301, 185 interactors |
ComplexPortali | CPX-2105, Cobalamin ABC transporter complex CPX-2106, BtuCD complex |
DIPi | DIP-9234N |
IntActi | P06611, 9 interactors |
MINTi | P06611 |
STRINGi | 511145.b1709 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P06611 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06611 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 233 | ABC transporterUniRule annotationAdd BLAST | 233 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily. Vitamin B12 importer (TC 3.A.1.13.1) family. [View classification]UniRule annotation
Phylogenomic databases
eggNOGi | COG4138, Bacteria |
HOGENOMi | CLU_000604_1_11_6 |
InParanoidi | P06611 |
PhylomeDBi | P06611 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
HAMAPi | MF_01005, BtuD, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR023693, ABC_transptr_BtuD IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P06611-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSIVMQLQDV AESTRLGPLS GEVRAGEILH LVGPNGAGKS TLLARMAGMT
60 70 80 90 100
SGKGSIQFAG QPLEAWSATK LALHRAYLSQ QQTPPFATPV WHYLTLHQHD
110 120 130 140 150
KTRTELLNDV AGALALDDKL GRSTNQLSGG EWQRVRLAAV VLQITPQANP
160 170 180 190 200
AGQLLLLDEP MNSLDVAQQS ALDKILSALC QQGLAIVMSS HDLNHTLRHA
210 220 230 240
HRAWLLKGGK MLASGRREEV LTPPNLAQAY GMNFRRLDIE GHRMLISTI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14031 Genomic DNA Translation: AAA23528.1 U00096 Genomic DNA Translation: AAC74779.1 AP009048 Genomic DNA Translation: BAA15477.1 |
PIRi | C24498, QRECBD |
RefSeqi | NP_416224.1, NC_000913.3 WP_000029474.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74779; AAC74779; b1709 BAA15477; BAA15477; BAA15477 |
GeneIDi | 945751 |
KEGGi | ecj:JW1699 eco:b1709 |
PATRICi | fig|1411691.4.peg.548 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M14031 Genomic DNA Translation: AAA23528.1 U00096 Genomic DNA Translation: AAC74779.1 AP009048 Genomic DNA Translation: BAA15477.1 |
PIRi | C24498, QRECBD |
RefSeqi | NP_416224.1, NC_000913.3 WP_000029474.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1L7V | X-ray | 3.20 | C/D | 1-249 | [»] | |
2QI9 | X-ray | 2.60 | C/D | 1-249 | [»] | |
4DBL | X-ray | 3.49 | C/D/H/I | 1-249 | [»] | |
4FI3 | X-ray | 3.47 | C/D | 1-249 | [»] | |
4R9U | X-ray | 2.78 | C/D | 1-249 | [»] | |
SMRi | P06611 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260301, 185 interactors |
ComplexPortali | CPX-2105, Cobalamin ABC transporter complex CPX-2106, BtuCD complex |
DIPi | DIP-9234N |
IntActi | P06611, 9 interactors |
MINTi | P06611 |
STRINGi | 511145.b1709 |
Protein family/group databases
TCDBi | 3.A.1.13.1, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P06611 |
PaxDbi | P06611 |
PRIDEi | P06611 |
Genome annotation databases
EnsemblBacteriai | AAC74779; AAC74779; b1709 BAA15477; BAA15477; BAA15477 |
GeneIDi | 945751 |
KEGGi | ecj:JW1699 eco:b1709 |
PATRICi | fig|1411691.4.peg.548 |
Organism-specific databases
EchoBASEi | EB0126 |
Phylogenomic databases
eggNOGi | COG4138, Bacteria |
HOGENOMi | CLU_000604_1_11_6 |
InParanoidi | P06611 |
PhylomeDBi | P06611 |
Enzyme and pathway databases
BioCyci | EcoCyc:BTUD-MONOMER |
BRENDAi | 7.6.2.8, 2026 |
Miscellaneous databases
EvolutionaryTracei | P06611 |
PROi | PR:P06611 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
HAMAPi | MF_01005, BtuD, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR023693, ABC_transptr_BtuD IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BTUD_ECOLI | |
Accessioni | P06611Primary (citable) accession number: P06611 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- UniProtKB entry view manual
User manual for the UniProtKB entry view - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families