Tegument protein VP16

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• activating transcription factor binding Source: CAFA <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • "The intrinsically unstructured domain of PC4 modulates the activity of the structured core through inter- and intramolecular interactions."
    Jonker H.R.A., Wechselberger R.W., Boelens R., Kaptein R., Folkers G.E.
    Biochemistry 45:5067-5081(2006) [PubMed] [Europe PMC] [Abstract]
• core promoter binding Source: CAFA <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Structural and functional properties of the N transcriptional activation domain of thyroid transcription factor-1: similarities with the acidic activation domains."
    Tell G., Perrone L., Fabbro D., Pellizzari L., Pucillo C., De Felice M., Acquaviva R., Formisano S., Damante G.
    Biochem. J. 329:395-403(1998) [PubMed] [Europe PMC] [Abstract]
• DNA binding Source: CAFA <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • Ref.12

    "Crystal structure of the conserved core of the herpes simplex virus transcriptional regulatory protein VP16."
    Liu Y., Gong W., Huang C.C., Herr W., Cheng X.
    Genes Dev. 13:1692-1703(1999) [PubMed] [Europe PMC] [Abstract]

    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 49-412, INTERACTION WITH HUMAN HCFC1 AND HUMAN POU2F1.
  • "Interdigitated residues within a small region of VP16 interact with Oct-1, HCF, and DNA."
    Lai J.S., Herr W.
    Mol. Cell. Biol. 17:3937-3946(1997) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription factor activity Source: CAFAInferred from mutant phenotypeⁱ
  • "Structural and functional properties of the N transcriptional activation domain of thyroid transcription factor-1: similarities with the acidic activation domains."
    Tell G., Perrone L., Fabbro D., Pellizzari L., Pucillo C., De Felice M., Acquaviva R., Formisano S., Damante G.
    Biochem. J. 329:395-403(1998) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase II proximal promoter sequence-specific DNA binding Source: CAFAInferred from direct assayⁱ
  • "Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator."
    Lee L., Stollar E., Chang J., Grossmann J.G., O'Brien R., Ladbury J., Carpenter B., Roberts S., Luisi B.
    Biochemistry 40:6580-6588(2001) [PubMed] [Europe PMC] [Abstract]
• structural constituent of virion Source: CAFAInferred from direct assayⁱ
  • Ref.12

    "Crystal structure of the conserved core of the herpes simplex virus transcriptional regulatory protein VP16."
    Liu Y., Gong W., Huang C.C., Herr W., Cheng X.
    Genes Dev. 13:1692-1703(1999) [PubMed] [Europe PMC] [Abstract]

    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 49-412, INTERACTION WITH HUMAN HCFC1 AND HUMAN POU2F1.
• transcription coactivator activity Source: CAFAInferred from direct assayⁱ
  • "Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator."
    Lee L., Stollar E., Chang J., Grossmann J.G., O'Brien R., Ladbury J., Carpenter B., Roberts S., Luisi B.
    Biochemistry 40:6580-6588(2001) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• DNA-templated viral transcription Source: UniProtKBInferred from direct assayⁱ
  • "Specific transcriptional activation in vitro by the herpes simplex virus protein VP16."
    Arnosti D.N., Preston C.M., Hagmann M., Schaffner W., Hope R.G., Laughlan G., Luisi B.F.
    Nucleic Acids Res. 21:5570-5576(1993) [PubMed] [Europe PMC] [Abstract]
• interaction with host Source: CAFAInferred from direct assayⁱ
  • "Compartmentalization of VP16 in cells infected with recombinant herpes simplex virus expressing VP16-green fluorescent protein fusion proteins."
    La Boissiere S., Izeta A., Malcomber S., O'Hare P.
    J. Virol. 78:8002-8014(2004) [PubMed] [Europe PMC] [Abstract]
• positive regulation of transcription, DNA-templated Source: CAFAInferred from mutant phenotypeⁱ
  • "Structural and functional properties of the N transcriptional activation domain of thyroid transcription factor-1: similarities with the acidic activation domains."
    Tell G., Perrone L., Fabbro D., Pellizzari L., Pucillo C., De Felice M., Acquaviva R., Formisano S., Damante G.
    Biochem. J. 329:395-403(1998) [PubMed] [Europe PMC] [Abstract]
• positive regulation of transcription by RNA polymerase II Source: CAFA <p>Inferred from Genetic Interaction</p> <p>Used to describe “traditional” genetic interactions such as suppressors and synthetic lethals as well as other techniques such as functional complementation, rescue experiments, or inferences about a gene drawn from the phenotype of a mutation in a different gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#igi">GO evidence code guide</a></p> Inferred from genetic interactionⁱ
  • "Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator."
    Lee L., Stollar E., Chang J., Grossmann J.G., O'Brien R., Ladbury J., Carpenter B., Roberts S., Luisi B.
    Biochemistry 40:6580-6588(2001) [PubMed] [Europe PMC] [Abstract]
• protein-containing complex assembly Source: CAFAInferred from direct assayⁱ
  • Ref.12

    "Crystal structure of the conserved core of the herpes simplex virus transcriptional regulatory protein VP16."
    Liu Y., Gong W., Huang C.C., Herr W., Cheng X.
    Genes Dev. 13:1692-1703(1999) [PubMed] [Europe PMC] [Abstract]

    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 49-412, INTERACTION WITH HUMAN HCFC1 AND HUMAN POU2F1.
• regulation of viral transcription Source: CAFAInferred from mutant phenotypeⁱ
  • "Interdigitated residues within a small region of VP16 interact with Oct-1, HCF, and DNA."
    Lai J.S., Herr W.
    Mol. Cell. Biol. 17:3937-3946(1997) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

GO - Cellular componentⁱ

• host cell cytoplasm Source: CAFAInferred from direct assayⁱ
  • "Compartmentalization of VP16 in cells infected with recombinant herpes simplex virus expressing VP16-green fluorescent protein fusion proteins."
    La Boissiere S., Izeta A., Malcomber S., O'Hare P.
    J. Virol. 78:8002-8014(2004) [PubMed] [Europe PMC] [Abstract]
• host cell cytoplasmic vesicle Source: CAFAInferred from direct assayⁱ
  • "Compartmentalization of VP16 in cells infected with recombinant herpes simplex virus expressing VP16-green fluorescent protein fusion proteins."
    La Boissiere S., Izeta A., Malcomber S., O'Hare P.
    J. Virol. 78:8002-8014(2004) [PubMed] [Europe PMC] [Abstract]
• host cell nucleus Source: CAFAInferred from direct assayⁱ
  • "Compartmentalization of VP16 in cells infected with recombinant herpes simplex virus expressing VP16-green fluorescent protein fusion proteins."
    La Boissiere S., Izeta A., Malcomber S., O'Hare P.
    J. Virol. 78:8002-8014(2004) [PubMed] [Europe PMC] [Abstract]
• host cell perinuclear region of cytoplasm Source: CAFAInferred from direct assayⁱ
  • "Compartmentalization of VP16 in cells infected with recombinant herpes simplex virus expressing VP16-green fluorescent protein fusion proteins."
    La Boissiere S., Izeta A., Malcomber S., O'Hare P.
    J. Virol. 78:8002-8014(2004) [PubMed] [Europe PMC] [Abstract]
• replication compartment Source: CAFAInferred from direct assayⁱ
  • "Compartmentalization of VP16 in cells infected with recombinant herpes simplex virus expressing VP16-green fluorescent protein fusion proteins."
    La Boissiere S., Izeta A., Malcomber S., O'Hare P.
    J. Virol. 78:8002-8014(2004) [PubMed] [Europe PMC] [Abstract]
• viral tegument Source: CAFAInferred from direct assayⁱ
  • "Structural analysis of herpes simplex virus by optical super-resolution imaging."
    Laine R.F., Albecka A., van de Linde S., Rees E.J., Crump C.M., Kaminski C.F.
    Nat Commun 6:5980-5980(2015) [PubMed] [Europe PMC] [Abstract]

Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

GO - Molecular functionⁱ

• activating transcription factor binding Source: CAFAInferred from physical interactionⁱ
  • "The intrinsically unstructured domain of PC4 modulates the activity of the structured core through inter- and intramolecular interactions."
    Jonker H.R.A., Wechselberger R.W., Boelens R., Kaptein R., Folkers G.E.
    Biochemistry 45:5067-5081(2006) [PubMed] [Europe PMC] [Abstract]

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Region

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domainⁱ

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MDLLVDELFA DMNADGASPP PPRPAGGPKN TPAAPPLYAT GRLSQAQLMP 
        60         70         80         90        100
SPPMPVPPAA LFNRLLDDLG FSAGPALCTM LDTWNEDLFS ALPTNADLYR 
       110        120        130        140        150
ECKFLSTLPS DVVEWGDAYV PERTQIDIRA HGDVAFPTLP ATRDGLGLYY 
       160        170        180        190        200
EALSRFFHAE LRAREESYRT VLANFCSALY RYLRASVRQL HRQAHMRGRD 
       210        220        230        240        250
RDLGEMLRAT IADRYYRETA RLARVLFLHL YLFLTREILW AAYAEQMMRP 
       260        270        280        290        300
DLFDCLCCDL ESWRQLAGLF QPFMFVNGAL TVRGVPIEAR RLRELNHIRE 
       310        320        330        340        350
HLNLPLVRSA ATEEPGAPLT TPPTLHGNQA RASGYFMVLI RAKLDSYSSF 
       360        370        380        390        400
TTSPSEAVMR EHAYSRARTK NNYGSTIEGL LDLPDDDAPE EAGLAAPRLS 
       410        420        430        440        450
FLPAGHTRRL STAPPTDVSL GDELHLDGED VAMAHADALD DFDLDMLGDG 
       460        470        480        490
DSPGPGFTPH DSAPYGALDM ADFEFEQMFT DALGIDEYGG            

Natural variant

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Proteomic databases

Genome annotation databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
2. SIMILARITY comments
   Index of protein domains and families