UL48

Functionⁱ

Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection.

May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis.Curated

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	442	Critical role in activation		1

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA-templated viral transcription
Source: UniProtKB
Inferred from direct assayⁱ
- 8284200
interaction with host
Source: CAFA
Inferred from direct assayⁱ
- 15254172
interaction with host via substance released outside of symbiont
Source: CAFA
Inferred from direct assayⁱ
- 9199328
positive regulation of transcription, DNA-templated
Source: CAFA
Inferred from mutant phenotypeⁱ
- 9425125
positive regulation of transcription by RNA polymerase II
Source: CAFA
Inferred from genetic interactionⁱ
- 11380252
protein-containing complex assembly
Source: CAFA
Inferred from direct assayⁱ
- 10398682
regulation of viral transcription
Source: CAFA
Inferred from mutant phenotypeⁱ
- 9199328
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Host-virus interaction, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Tegument protein VP16 Alternative name(s): Alpha trans-inducing protein Alpha-TIF ICP25 Vmw65
Gene namesⁱ	ORF Names:UL48
Organismⁱ	Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifierⁱ	10299 [NCBI]
Taxonomic lineageⁱ	Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1
Virus hostⁱ	Homo sapiens (Human) [TaxID: 9606]
Proteomesⁱ	UP000009294 Componentⁱ: Genome UP000180652 Componentⁱ: Genome

Subcellular locationⁱ

GO - Cellular componentⁱ

host cell cytoplasm
Source: CAFA
Inferred from direct assayⁱ
- 15254172
host cell cytoplasmic vesicle
Source: CAFA
Inferred from direct assayⁱ
- 15254172
host cell nucleus
Source: CAFA
Inferred from direct assayⁱ
- 15254172
host cell perinuclear region of cytoplasm
Source: CAFA
Inferred from direct assayⁱ
- 15254172
replication compartment
Source: CAFA
Inferred from direct assayⁱ
- 15254172
viral tegument
Source: CAFA
Inferred from direct assayⁱ
- 25609143

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Host nucleus, Virion, Virion tegument

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL4218

ChEMBLⁱ

CHEMBL4218

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000115798	1 – 490	Tegument protein VP16Add BLAST		490

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	18	Phosphoserine1 Publication	1
Modified residueⁱ	353	Phosphoserine1 Publication	1
Modified residueⁱ	411	Phosphoserine1 Publication	1
Modified residueⁱ	452	Phosphoserine1 Publication	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PRIDEⁱ	P06492

PRIDEⁱ

P06492

PTM databases

iPTMnetⁱ	P06492

iPTMnetⁱ

P06492

Interactionⁱ

Subunit structureⁱ

Interacts with VP22. Interacts with gH (via C-terminus). Interacts with the virion host shutoff protein (vhs). Interacts with VP11/12. Associates with the VP16-induced complex; binding to host HCFC1 activates VP16 for association with the octamer motif-binding host protein POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes.5 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
MED25	Q7XYY2	2	EBI-7489933,EBI-1386292	From Arabidopsis thaliana.
UL41	P10225	3	EBI-7489933,EBI-6148417
UL46	P10230	2	EBI-7489933,EBI-7694458
UL49	P10233	2	EBI-7489933,EBI-7490002

Show more details

GO - Molecular functionⁱ

activating transcription factor binding
Source: CAFA
Inferred from physical interactionⁱ
- 16605275

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	971443, 13 interactors
Database of interacting proteins More...DIPⁱ	DIP-41776N
ELMⁱ	P06492
IntActⁱ	P06492, 9 interactors
Molecular INTeraction database More...MINTⁱ	P06492

Chemistry databases

BindingDBⁱ

P06492

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	58 – 69	Combined sources	12
Helixⁱ	74 – 83	Combined sources	10
Helixⁱ	96 – 99	Combined sources	4
Helixⁱ	103 – 105	Combined sources	3
Helixⁱ	109 – 118	Combined sources	10
Helixⁱ	143 – 145	Combined sources	3
Helixⁱ	146 – 196	Combined sources	51
Helixⁱ	203 – 247	Combined sources	45
Helixⁱ	250 – 253	Combined sources	4
Beta strandⁱ	256 – 261	Combined sources	6
Helixⁱ	264 – 267	Combined sources	4
Beta strandⁱ	278 – 282	Combined sources	5
Helixⁱ	289 – 301	Combined sources	13
Turnⁱ	310 – 312	Combined sources	3
Helixⁱ	332 – 344	Combined sources	13
Beta strandⁱ	398 – 401	Combined sources	4
Turnⁱ	466 – 469	Combined sources	4
Helixⁱ	470 – 473	Combined sources	4
Helixⁱ	475 – 479	Combined sources	5
Turnⁱ	480 – 482	Combined sources	3
Beta strandⁱ	483 – 485	Combined sources	3
Helixⁱ	486 – 488	Combined sources	3

3D structure databases

ProteinModelPortalⁱ	P06492
SMRⁱ	P06492
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P06492

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	411 – 490	Transcriptional activationAdd BLAST		80

Domainⁱ

The transcriptional activation region seems to target many proteins of the RNA polymerase II transcription machinery.

Sequence similaritiesⁱ

Belongs to the herpesviridae tegument protein VP16 protein family.Curated

Phylogenomic databases

KEGG Orthology (KO) More...KOⁱ	K19444
Database of Orthologous Groups More...OrthoDBⁱ	VOG09000087

Family and domain databases

Gene3Dⁱ	1.10.1290.10, 1 hit
InterProⁱ	View protein in InterPro IPR003174 Alpha_TIF IPR036538 Alpha_TIF_sf IPR021051 HSV_VP16_C
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02232 Alpha_TIF, 1 hit PF12149 HSV_VP16_C, 1 hit
SMARTⁱ	View protein in SMART SM00814 Alpha_TIF, 1 hit
SUPFAMⁱ	SSF56548 SSF56548, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P06492-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDLLVDELFA DMNADGASPP PPRPAGGPKN TPAAPPLYAT GRLSQAQLMP 
        60         70         80         90        100
SPPMPVPPAA LFNRLLDDLG FSAGPALCTM LDTWNEDLFS ALPTNADLYR 
       110        120        130        140        150
ECKFLSTLPS DVVEWGDAYV PERTQIDIRA HGDVAFPTLP ATRDGLGLYY 
       160        170        180        190        200
EALSRFFHAE LRAREESYRT VLANFCSALY RYLRASVRQL HRQAHMRGRD 
       210        220        230        240        250
RDLGEMLRAT IADRYYRETA RLARVLFLHL YLFLTREILW AAYAEQMMRP 
       260        270        280        290        300
DLFDCLCCDL ESWRQLAGLF QPFMFVNGAL TVRGVPIEAR RLRELNHIRE 
       310        320        330        340        350
HLNLPLVRSA ATEEPGAPLT TPPTLHGNQA RASGYFMVLI RAKLDSYSSF 
       360        370        380        390        400
TTSPSEAVMR EHAYSRARTK NNYGSTIEGL LDLPDDDAPE EAGLAAPRLS 
       410        420        430        440        450
FLPAGHTRRL STAPPTDVSL GDELHLDGED VAMAHADALD DFDLDMLGDG 
       460        470        480        490
DSPGPGFTPH DSAPYGALDM ADFEFEQMFT DALGIDEYGG

Length:490

Mass (Da):54,345

Last modified:January 1, 1988 - v1

Checksum:ⁱ8DDDEDEDB2A699D3

GO

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱ	14	A → S in strain: Nonneuroinvasive mutant HF10.	1
Natural variantⁱ	124	T → A in strain: Nonneuroinvasive mutant HF10.	1
Natural variantⁱ	235	T → A in strain: 17 syn+.	1
Natural variantⁱ	390	E → K in strain: Nonneuroinvasive mutant HF10.	1
Natural variantⁱ	413	A → P in strain: Nonneuroinvasive mutant HF10.	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X14112 Genomic DNA Translation: CAA32298.1 X03141 Genomic DNA Translation: CAA26913.1 DQ889502 Genomic DNA Translation: ABI63509.1 FJ593289 Genomic DNA Translation: ACM62271.1
PIRⁱ	A24118 IXBE17
NCBI Reference Sequences More...RefSeqⁱ	YP_009137123.1, NC_001806.2

Genome annotation databases

GeneIDⁱ	24271473
KEGGⁱ	vg:24271473

Protein	Similar proteins		Species	Score	Length
P06492	Transactivating tegument protein VP16		HHV1		490
Tegument protein VP16	)	HHV1F		490
Tegument protein VP16		HHV11		490
Transactivating tegument protein VP16		Herpes simplex virus (type 1 /strain RH2)		490
Transactivating tegument protein VP16		HHV1		526
+24

Protein	Similar proteins		Species	Score	Length
P06492	Transactivating tegument protein VP16		HHV1		490
Tegument protein VP16		HHV11		490
Tegument protein VP16	)	HHV1F		490
Transactivating tegument protein VP16		Herpes simplex virus (type 1 /strain RH2)		490
Transactivating tegument protein VP16		HHV2		590
+59

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X14112 Genomic DNA Translation: CAA32298.1 X03141 Genomic DNA Translation: CAA26913.1 DQ889502 Genomic DNA Translation: ABI63509.1 FJ593289 Genomic DNA Translation: ACM62271.1
PIRⁱ	A24118 IXBE17
NCBI Reference Sequences More...RefSeqⁱ	YP_009137123.1, NC_001806.2

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	16VP	X-ray	2.10	A	47-412	[»]
	2PHE	NMR	-	C	465-490	[»]
	2PHG	NMR	-	B	465-490	[»]
ProteinModelPortalⁱ	P06492
SMRⁱ	P06492
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	971443, 13 interactors
Database of interacting proteins More...DIPⁱ	DIP-41776N
ELMⁱ	P06492
IntActⁱ	P06492, 9 interactors
Molecular INTeraction database More...MINTⁱ	P06492

Chemistry databases

BindingDBⁱ	P06492
ChEMBLⁱ	CHEMBL4218

PTM databases

iPTMnetⁱ	P06492

iPTMnetⁱ

P06492

Proteomic databases

PRIDEⁱ	P06492

PRIDEⁱ

P06492

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	24271473
KEGGⁱ	vg:24271473

Phylogenomic databases

KEGG Orthology (KO) More...KOⁱ	K19444
Database of Orthologous Groups More...OrthoDBⁱ	VOG09000087

Miscellaneous databases

EvolutionaryTraceⁱ	P06492
Protein Ontology More...PROⁱ	PR:P06492

Family and domain databases

Gene3Dⁱ	1.10.1290.10, 1 hit
InterProⁱ	View protein in InterPro IPR003174 Alpha_TIF IPR036538 Alpha_TIF_sf IPR021051 HSV_VP16_C
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02232 Alpha_TIF, 1 hit PF12149 HSV_VP16_C, 1 hit
SMARTⁱ	View protein in SMART SM00814 Alpha_TIF, 1 hit
SUPFAMⁱ	SSF56548 SSF56548, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	VP16_HHV11
Accessionⁱ	P06492Primary (citable) accession number: P06492 Secondary accession number(s): B9VQH6, Q09I86
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
	Last sequence update:	January 1, 1988
	Last modified:	July 18, 2018
	This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P06492 (VP16_HHV11)

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Tegument protein VP16

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Natural variant

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ