UniProtKB - P06492 (VP16_HHV11)
Tegument protein VP16
UL48
Functioni
Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection.
May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis.
CuratedSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 442 | Critical role in activation | 1 |
GO - Molecular functioni
- core promoter sequence-specific DNA binding Source: CAFA
- DNA binding Source: CAFA
- DNA-binding transcription factor activity Source: CAFA
- DNA-binding transcription factor binding Source: CAFA
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: CAFA
- structural constituent of virion Source: CAFA
- transcription coactivator activity Source: CAFA
GO - Biological processi
- biological process involved in interaction with host Source: CAFA
- DNA-templated viral transcription Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: CAFA
- positive regulation of transcription by RNA polymerase II Source: CAFA
- protein-containing complex assembly Source: CAFA
- regulation of viral transcription Source: CAFA
Keywordsi
Molecular function | DNA-binding |
Biological process | Host-virus interaction, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Tegument protein VP16Alternative name(s): Alpha trans-inducing protein Alpha-TIF ICP25 Vmw65 |
Gene namesi | ORF Names:UL48 |
Organismi | Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) |
Taxonomic identifieri | 10299 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Peploviricota › Herviviricetes › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
Other locations
- Virion tegument By similarity
- Host nucleus By similarity
Other locations
- host cell cytoplasm Source: CAFA
- host cell cytoplasmic vesicle Source: CAFA
- host cell nucleus Source: CAFA
- host cell perinuclear region of cytoplasm Source: CAFA
- replication compartment Source: CAFA
- viral tegument Source: CAFA
Keywords - Cellular componenti
Host nucleus, Virion, Virion tegumentPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000115798 | 1 – 490 | Tegument protein VP16Add BLAST | 490 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 18 | Phosphoserine1 Publication | 1 | |
Modified residuei | 353 | Phosphoserine1 Publication | 1 | |
Modified residuei | 411 | Phosphoserine1 Publication | 1 | |
Modified residuei | 452 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | P06492 |
PTM databases
iPTMneti | P06492 |
Interactioni
Subunit structurei
Interacts with VP22.
Interacts with gH (via C-terminus).
Interacts with the virion host shutoff protein (vhs).
Interacts with VP11/12. Associates with the VP16-induced complex; binding to host HCFC1 activates VP16 for association with the octamer motif-binding host protein POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes.
5 PublicationsBinary interactionsi
P06492
With | #Exp. | IntAct |
---|---|---|
UL41 [P10225] | 3 | EBI-7489933,EBI-6148417 |
UL46 [P10230] | 2 | EBI-7489933,EBI-7694458 |
UL49 [P10233] | 2 | EBI-7489933,EBI-7490002 |
MED25 [Q7XYY2] from Arabidopsis thaliana. | 2 | EBI-7489933,EBI-1386292 |
GO - Molecular functioni
- DNA-binding transcription factor binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 971443, 17 interactors |
DIPi | DIP-41776N |
ELMi | P06492 |
IntActi | P06492, 9 interactors |
MINTi | P06492 |
Chemistry databases
BindingDBi | P06492 |
Structurei
Secondary structure
3D structure databases
BMRBi | P06492 |
SMRi | P06492 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06492 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 12 – 35 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 411 – 490 | Transcriptional activationAdd BLAST | 80 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 17 – 31 | Pro residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Family and domain databases
DisProti | DP01642 |
Gene3Di | 1.10.1290.10, 1 hit |
IDEALi | IID90007 |
InterProi | View protein in InterPro IPR003174, Alpha_TIF IPR036538, Alpha_TIF_sf IPR021051, HSV_VP16_C |
Pfami | View protein in Pfam PF02232, Alpha_TIF, 1 hit PF12149, HSV_VP16_C, 1 hit |
SMARTi | View protein in SMART SM00814, Alpha_TIF, 1 hit |
SUPFAMi | SSF56548, SSF56548, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDLLVDELFA DMNADGASPP PPRPAGGPKN TPAAPPLYAT GRLSQAQLMP
60 70 80 90 100
SPPMPVPPAA LFNRLLDDLG FSAGPALCTM LDTWNEDLFS ALPTNADLYR
110 120 130 140 150
ECKFLSTLPS DVVEWGDAYV PERTQIDIRA HGDVAFPTLP ATRDGLGLYY
160 170 180 190 200
EALSRFFHAE LRAREESYRT VLANFCSALY RYLRASVRQL HRQAHMRGRD
210 220 230 240 250
RDLGEMLRAT IADRYYRETA RLARVLFLHL YLFLTREILW AAYAEQMMRP
260 270 280 290 300
DLFDCLCCDL ESWRQLAGLF QPFMFVNGAL TVRGVPIEAR RLRELNHIRE
310 320 330 340 350
HLNLPLVRSA ATEEPGAPLT TPPTLHGNQA RASGYFMVLI RAKLDSYSSF
360 370 380 390 400
TTSPSEAVMR EHAYSRARTK NNYGSTIEGL LDLPDDDAPE EAGLAAPRLS
410 420 430 440 450
FLPAGHTRRL STAPPTDVSL GDELHLDGED VAMAHADALD DFDLDMLGDG
460 470 480 490
DSPGPGFTPH DSAPYGALDM ADFEFEQMFT DALGIDEYGG
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 14 | A → S in strain: Nonneuroinvasive mutant HF10. | 1 | |
Natural varianti | 124 | T → A in strain: Nonneuroinvasive mutant HF10. | 1 | |
Natural varianti | 235 | T → A in strain: 17 syn+. | 1 | |
Natural varianti | 390 | E → K in strain: Nonneuroinvasive mutant HF10. | 1 | |
Natural varianti | 413 | A → P in strain: Nonneuroinvasive mutant HF10. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14112 Genomic DNA Translation: CAA32298.1 X03141 Genomic DNA Translation: CAA26913.1 DQ889502 Genomic DNA Translation: ABI63509.1 FJ593289 Genomic DNA Translation: ACM62271.1 |
PIRi | A24118, IXBE17 |
RefSeqi | YP_009137123.1, NC_001806.2 |
Genome annotation databases
GeneIDi | 24271473 |
KEGGi | vg:24271473 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14112 Genomic DNA Translation: CAA32298.1 X03141 Genomic DNA Translation: CAA26913.1 DQ889502 Genomic DNA Translation: ABI63509.1 FJ593289 Genomic DNA Translation: ACM62271.1 |
PIRi | A24118, IXBE17 |
RefSeqi | YP_009137123.1, NC_001806.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
16VP | X-ray | 2.10 | A | 47-412 | [»] | |
2PHE | NMR | - | C | 465-490 | [»] | |
2PHG | NMR | - | B | 465-490 | [»] | |
BMRBi | P06492 | |||||
SMRi | P06492 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 971443, 17 interactors |
DIPi | DIP-41776N |
ELMi | P06492 |
IntActi | P06492, 9 interactors |
MINTi | P06492 |
Chemistry databases
BindingDBi | P06492 |
ChEMBLi | CHEMBL4218 |
PTM databases
iPTMneti | P06492 |
Proteomic databases
PRIDEi | P06492 |
Protocols and materials databases
DNASUi | 24271473 |
Genome annotation databases
GeneIDi | 24271473 |
KEGGi | vg:24271473 |
Miscellaneous databases
EvolutionaryTracei | P06492 |
PROi | PR:P06492 |
Family and domain databases
DisProti | DP01642 |
Gene3Di | 1.10.1290.10, 1 hit |
IDEALi | IID90007 |
InterProi | View protein in InterPro IPR003174, Alpha_TIF IPR036538, Alpha_TIF_sf IPR021051, HSV_VP16_C |
Pfami | View protein in Pfam PF02232, Alpha_TIF, 1 hit PF12149, HSV_VP16_C, 1 hit |
SMARTi | View protein in SMART SM00814, Alpha_TIF, 1 hit |
SUPFAMi | SSF56548, SSF56548, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VP16_HHV11 | |
Accessioni | P06492Primary (citable) accession number: P06492 Secondary accession number(s): B9VQH6, Q09I86 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | September 29, 2021 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families