UniProtKB - P06474 (RIR2_HHV1K)
Protein
Ribonucleoside-diphosphate reductase small subunit
Gene
RIR2
Organism
Human herpesvirus 1 (strain KOS) (HHV-1) (Human herpes simplex virus 1)
Status
Functioni
Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation
Catalytic activityi
- [thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphateUniRule annotationEC:1.17.4.1UniRule annotation
Cofactori
Fe cationUniRule annotation
: DNA replication Pathwayi
This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotationView all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 94 | Iron 1UniRule annotation | 1 | |
Metal bindingi | 124 | Iron 1UniRule annotation | 1 | |
Metal bindingi | 124 | Iron 2UniRule annotation | 1 | |
Metal bindingi | 127 | Iron 1UniRule annotation | 1 | |
Active sitei | 131 | UniRule annotation | 1 | |
Metal bindingi | 187 | Iron 2UniRule annotation | 1 | |
Metal bindingi | 221 | Iron 2UniRule annotation | 1 | |
Metal bindingi | 224 | Iron 2UniRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-UniRule
- ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-UniRule
GO - Biological processi
- deoxyribonucleoside diphosphate metabolic process Source: UniProtKB-UniRule
- deoxyribonucleotide biosynthetic process Source: InterPro
- DNA replication Source: UniProtKB-UniRule
- release from viral latency Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | DNA replication, Viral latency, Viral reactivation from latency |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00326 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonucleoside-diphosphate reductase small subunitUniRule annotation (EC:1.17.4.1UniRule annotation)Alternative name(s): Ribonucleotide reductase small subunitUniRule annotation |
Gene namesi | Name:RIR2UniRule annotation Synonyms:UL40 |
Organismi | Human herpesvirus 1 (strain KOS) (HHV-1) (Human herpes simplex virus 1) |
Taxonomic identifieri | 10306 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Peploviricota › Herviviricetes › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Subcellular locationi
- Host membrane UniRule annotation; Single-pass membrane protein UniRule annotation
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 180 – 200 | HelicalUniRule annotationAdd BLAST | 21 |
GO - Cellular componenti
- host cell membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-UniRule
Keywords - Cellular componenti
Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000190504 | 1 – 340 | Ribonucleoside-diphosphate reductase small subunitAdd BLAST | 340 |
Interactioni
Subunit structurei
Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.
UniRule annotationFamily & Domainsi
Sequence similaritiesi
Belongs to the ribonucleoside diphosphate reductase small chain family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd01049, RNRR2, 1 hit |
Gene3Di | 1.10.620.20, 1 hit |
HAMAPi | MF_04028, HSV_RIR2, 1 hit |
InterProi | View protein in InterPro IPR009078, Ferritin-like_SF IPR034715, HSV_RIR2 IPR012348, RNR-like IPR033909, RNR_small IPR030475, RNR_small_AS IPR000358, RNR_small_fam |
PANTHERi | PTHR23409, PTHR23409, 1 hit |
Pfami | View protein in Pfam PF00268, Ribonuc_red_sm, 1 hit |
SUPFAMi | SSF47240, SSF47240, 1 hit |
PROSITEi | View protein in PROSITE PS00368, RIBORED_SMALL, 1 hit |
i Sequence
Sequence statusi: Complete.
P06474-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDSAAPALSP ALTAHTGHSA TADLAIQIPK CPDPERYFYT SQCPDINHLR
60 70 80 90 100
SLSILNRWLE TELVFVGDEE DVSKLSEGEL SFYRFLFAFL SAADDLVTEN
110 120 130 140 150
LGGLSGLFEQ KDILHYYVEQ ECIEVAHSRV YNIIQLVLFH NNDQARREYV
160 170 180 190 200
AGTINHPAIR AKVDWLEARV RECASVPEKF ILMILIEGIF FAASFAAIAY
210 220 230 240 250
LRTNNLLRVT CQSNDLISRD EAVHTTASCY IYNNYLGGHA KPPPDRVYGL
260 270 280 290 300
FRQAVEIEIG FIRSQAPTDS HILSPAALAA IENYVRFSAD RLLGLIHMKP
310 320 330 340
LFSAPPPDAS FPLSLMSTDK HTNFFECRST SYAGAVVNDL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02212 Genomic DNA Translation: AAA66436.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02212 Genomic DNA Translation: AAA66436.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Enzyme and pathway databases
UniPathwayi | UPA00326 |
Family and domain databases
CDDi | cd01049, RNRR2, 1 hit |
Gene3Di | 1.10.620.20, 1 hit |
HAMAPi | MF_04028, HSV_RIR2, 1 hit |
InterProi | View protein in InterPro IPR009078, Ferritin-like_SF IPR034715, HSV_RIR2 IPR012348, RNR-like IPR033909, RNR_small IPR030475, RNR_small_AS IPR000358, RNR_small_fam |
PANTHERi | PTHR23409, PTHR23409, 1 hit |
Pfami | View protein in Pfam PF00268, Ribonuc_red_sm, 1 hit |
SUPFAMi | SSF47240, SSF47240, 1 hit |
PROSITEi | View protein in PROSITE PS00368, RIBORED_SMALL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RIR2_HHV1K | |
Accessioni | P06474Primary (citable) accession number: P06474 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families