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Protein

Replication protein E1

Gene

E1

Organism
Human papillomavirus type 33
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi470 – 477ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication protein E1UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase E1UniRule annotation
Gene namesi
Name:E1UniRule annotation
OrganismiHuman papillomavirus type 33
Taxonomic identifieri10586 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeFirstpapillomavirinaeAlphapapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009118 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001331311 – 644Replication protein E1Add BLAST644

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki551Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)UniRule annotation

Post-translational modificationi

Phosphorylated.UniRule annotation
Sumoylated.UniRule annotation

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA-binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP06421
SMRiP06421
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini444 – 594SF3 helicaseUniRule annotationAdd BLAST151

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni179 – 345DNA-binding regionUniRule annotationAdd BLAST167
Regioni312 – 644Required for E2 bindingAdd BLAST333

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi86 – 88Nuclear localization signalUniRule annotation3

Sequence similaritiesi

Belongs to the papillomaviridae E1 protein family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG090000HF

Family and domain databases

HAMAPiMF_04000 PPV_E1, 1 hit
InterProiView protein in InterPro
IPR001177 DNA_helicase_E1_C_Papillomavir
IPR014000 DNA_helicase_E1_N_Papillomavir
IPR014015 Helicase_SF3_DNA-vir
IPR027417 P-loop_NTPase
IPR016393 Rep_E1_papillomaV
PfamiView protein in Pfam
PF00519 PPV_E1_C, 1 hit
PF00524 PPV_E1_N, 1 hit
PIRSFiPIRSF003383 Rep_E1_papillomaV, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51206 SF3_HELICASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P06421-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADPEGTNGA GMGCTGWFEV EAVIERRTGD NISEDEDETA DDSGTDLLEF
60 70 80 90 100
IDDSMENSIQ ADTEAARALF NIQEGEDDLN AVCALKRKFA ACSQSAAEDV
110 120 130 140 150
VDRAANPCRT SINKNKECTY RKRKIDELED SGYGNTEVET QQMVQQVESQ
160 170 180 190 200
NGDTNLNDLE SSGVGDDSEV SCETNVDSCE NVTLQEISNV LHSSNTKANI
210 220 230 240 250
LYKFKEAYGI SFMELVRPFK SDKTSCTDWC ITGYGISPSV AESLKVLIKQ
260 270 280 290 300
HSLYTHLQCL TCDRGIIILL LIRFRCSKNR LTVAKLMSNL LSIPETCMVI
310 320 330 340 350
EPPKLRSQTC ALYWFRTAMS NISDVQGTTP EWIDRLTVLQ HSFNDNIFDL
360 370 380 390 400
SEMVQWAYDN ELTDDSDIAY YYAQLADSNS NAAAFLKSNS QAKIVKDCGI
410 420 430 440 450
MCRHYKKAEK RKMSIGQWIQ SRCEKTNDGG NWRPIVQLLR YQNIEFTAFL
460 470 480 490 500
GAFKKFLKGI PKKSCMLICG PANTGKSYFG MSLIQFLKGC VISCVNSKSH
510 520 530 540 550
FWLQPLSDAK IGMIDDVTPI SWTYIDDYMR NALDGNEISI DVKHRALVQL
560 570 580 590 600
KCPPLLLTSN TNAGTDSRWP YLHSRLTVFE FKNPFPFDEN GNPVYAINDE
610 620 630 640
NWKSFFSRTW CKLDLIEEED KENHGGNIST FKCSAGENTR SLRS
Length:644
Mass (Da):72,469
Last modified:January 1, 1988 - v1
Checksum:iF6F5A93C9E32D201
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12732 Genomic DNA Translation: AAA46960.1
PIRiA03660 W1WL33

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12732 Genomic DNA Translation: AAA46960.1
PIRiA03660 W1WL33

3D structure databases

ProteinModelPortaliP06421
SMRiP06421
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG090000HF

Family and domain databases

HAMAPiMF_04000 PPV_E1, 1 hit
InterProiView protein in InterPro
IPR001177 DNA_helicase_E1_C_Papillomavir
IPR014000 DNA_helicase_E1_N_Papillomavir
IPR014015 Helicase_SF3_DNA-vir
IPR027417 P-loop_NTPase
IPR016393 Rep_E1_papillomaV
PfamiView protein in Pfam
PF00519 PPV_E1_C, 1 hit
PF00524 PPV_E1_N, 1 hit
PIRSFiPIRSF003383 Rep_E1_papillomaV, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51206 SF3_HELICASE_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVE1_HPV33
AccessioniPrimary (citable) accession number: P06421
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 10, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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