Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Renin-1

Gene

Ren1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Catalytic activityi

Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

Activity regulationi

Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102PROSITE-ProRule annotation1
Active sitei287PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

ReactomeiR-MMU-2022377 Metabolism of Angiotensinogen to Angiotensins

Names & Taxonomyi

Protein namesi
Recommended name:
Renin-1 (EC:3.4.23.15)
Alternative name(s):
Angiotensinogenase
Kidney renin
Gene namesi
Name:Ren1
Synonyms:Ren, Ren-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:97898 Ren1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2615

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
PropeptideiPRO_000002608922 – 71Activation peptide1 PublicationAdd BLAST50
ChainiPRO_000002609072 – 402Renin-1Add BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi69N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi115 ↔ 122By similarity
Glycosylationi139N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi278 ↔ 282By similarity
Glycosylationi320N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP06281
PaxDbiP06281
PeptideAtlasiP06281
PRIDEiP06281

PTM databases

PhosphoSitePlusiP06281

Expressioni

Tissue specificityi

Kidney.

Inductioni

Renal renin is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration.

Gene expression databases

BgeeiENSMUSG00000070645 Expressed in 82 organ(s), highest expression level in submandibular gland
CleanExiMM_REN1
ExpressionAtlasiP06281 baseline and differential
GenevisibleiP06281 MM

Interactioni

Subunit structurei

Interacts with ATP6AP2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP06281, 1 interactor
MINTiP06281
STRINGi10090.ENSMUSP00000092135

Chemistry databases

BindingDBiP06281

Structurei

Secondary structure

1402
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP06281
SMRiP06281
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini84 – 399Peptidase A1PROSITE-ProRule annotationAdd BLAST316

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
GeneTreeiENSGT00760000118929
HOGENOMiHOG000197681
HOVERGENiHBG000482
InParanoidiP06281
KOiK01380
OMAiLGKYYTE
OrthoDBiEOG091G0JP7
PhylomeDBiP06281
TreeFamiTF314990

Family and domain databases

CDDicd05487 renin_like, 1 hit
Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
IPR034135 Renin-like_dom
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P06281-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDRRRMPLWA LLLLWSPCTF SLPTRTATFE RIPLKKMPSV REILEERGVD
60 70 80 90 100
MTRLSAEWGV FTKRPSLTNL TSPVVLTNYL NTQYYGEIGI GTPPQTFKVI
110 120 130 140 150
FDTGSANLWV PSTKCSRLYL ACGIHSLYES SDSSSYMENG SDFTIHYGSG
160 170 180 190 200
RVKGFLSQDS VTVGGITVTQ TFGEVTELPL IPFMLAKFDG VLGMGFPAQA
210 220 230 240 250
VGGVTPVFDH ILSQGVLKEE VFSVYYNRGS HLLGGEVVLG GSDPQHYQGN
260 270 280 290 300
FHYVSISKTD SWQITMKGVS VGSSTLLCEE GCAVVVDTGS SFISAPTSSL
310 320 330 340 350
KLIMQALGAK EKRIEEYVVN CSQVPTLPDI SFDLGGRAYT LSSTDYVLQY
360 370 380 390 400
PNRRDKLCTL ALHAMDIPPP TGPVWVLGAT FIRKFYTEFD RHNNRIGFAL

AR
Length:402
Mass (Da):44,343
Last modified:January 1, 1988 - v1
Checksum:iD42920B555E97A38
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q9R259Q9R259_MOUSE
Renin b
Ren1
44Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6 – 23Missing (PubMed:6370686).CuratedAdd BLAST18
Sequence conflicti24T → I (PubMed:6370686).Curated1
Sequence conflicti163V → VSRV (PubMed:6370686).Curated1

Polymorphismi

In inbred mouse strains, there are at least two alleles which can occur at the Ren1 locus: Ren-1D and Ren-1C. The sequence shown is that of Ren-1C.
Present as a single-copy gene in strains such as BALB/c and C57BL/6 while some strains such as Swiss and Akr contain two copies.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti58W → R in allele Ren-1D. 1 Publication1
Natural varianti68T → I in allele Ren-1D. 1 Publication1
Natural varianti160S → V in allele Ren-1D; requires 2 nucleotide substitutions. 1 Publication1
Natural varianti315E → D in allele Ren-1D. 2 Publications1
Natural varianti352N → Y in allele Ren-1D. Combined sources1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00810
, X00811, X00812, X00813, X00814, X00815, X00816, X00850, X00851 Genomic DNA Translation: CAA25391.1
X16642 mRNA Translation: CAA34636.1
K02596 Genomic DNA Translation: AAA40045.1
M32352 Genomic DNA Translation: AAA40043.1
AK052685 mRNA Translation: BAC35094.1
AK085309 mRNA Translation: BAC39418.1
BC061053 mRNA Translation: AAH61053.1
K02800 Genomic DNA Translation: AAA40044.1
M34190 Genomic DNA Translation: AAA40042.1
CCDSiCCDS15295.1
PIRiA00989 REMSK
RefSeqiNP_112469.1, NM_031192.3
UniGeneiMm.220955

Genome annotation databases

EnsembliENSMUST00000094556; ENSMUSP00000092135; ENSMUSG00000070645
GeneIDi19701
KEGGimmu:19701
UCSCiuc007cqf.1 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00810
, X00811, X00812, X00813, X00814, X00815, X00816, X00850, X00851 Genomic DNA Translation: CAA25391.1
X16642 mRNA Translation: CAA34636.1
K02596 Genomic DNA Translation: AAA40045.1
M32352 Genomic DNA Translation: AAA40043.1
AK052685 mRNA Translation: BAC35094.1
AK085309 mRNA Translation: BAC39418.1
BC061053 mRNA Translation: AAH61053.1
K02800 Genomic DNA Translation: AAA40044.1
M34190 Genomic DNA Translation: AAA40042.1
CCDSiCCDS15295.1
PIRiA00989 REMSK
RefSeqiNP_112469.1, NM_031192.3
UniGeneiMm.220955

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MKTX-ray3.20A22-402[»]
ProteinModelPortaliP06281
SMRiP06281
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP06281, 1 interactor
MINTiP06281
STRINGi10090.ENSMUSP00000092135

Chemistry databases

BindingDBiP06281
ChEMBLiCHEMBL2615

PTM databases

PhosphoSitePlusiP06281

Proteomic databases

MaxQBiP06281
PaxDbiP06281
PeptideAtlasiP06281
PRIDEiP06281

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094556; ENSMUSP00000092135; ENSMUSG00000070645
GeneIDi19701
KEGGimmu:19701
UCSCiuc007cqf.1 mouse

Organism-specific databases

CTDi19701
MGIiMGI:97898 Ren1

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
GeneTreeiENSGT00760000118929
HOGENOMiHOG000197681
HOVERGENiHBG000482
InParanoidiP06281
KOiK01380
OMAiLGKYYTE
OrthoDBiEOG091G0JP7
PhylomeDBiP06281
TreeFamiTF314990

Enzyme and pathway databases

ReactomeiR-MMU-2022377 Metabolism of Angiotensinogen to Angiotensins

Miscellaneous databases

PROiPR:P06281
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000070645 Expressed in 82 organ(s), highest expression level in submandibular gland
CleanExiMM_REN1
ExpressionAtlasiP06281 baseline and differential
GenevisibleiP06281 MM

Family and domain databases

CDDicd05487 renin_like, 1 hit
Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
IPR034135 Renin-like_dom
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRENI1_MOUSE
AccessioniPrimary (citable) accession number: P06281
Secondary accession number(s): P97911
, Q543E5, Q62153, Q62154
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 12, 2018
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again