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UniProtKB - P06281 (RENI1_MOUSE)
Protein
Renin-1
Gene
Ren1
Organism
Mus musculus (Mouse)
Status
Functioni
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Catalytic activityi
- Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I. EC:3.4.23.15
Activity regulationi
Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 102 | PROSITE-ProRule annotation | 1 | |
Active sitei | 287 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: MGI
- endopeptidase activity Source: MGI
- insulin-like growth factor receptor binding Source: MGI
- peptidase activity Source: MGI
- signaling receptor binding Source: MGI
GO - Biological processi
- amyloid-beta metabolic process Source: Ensembl
- angiotensin maturation Source: MGI
- cell maturation Source: MGI
- cellular response to xenobiotic stimulus Source: Ensembl
- drinking behavior Source: MGI
- hormone-mediated signaling pathway Source: MGI
- kidney development Source: MGI
- male gonad development Source: UniProtKB
- mesonephros development Source: UniProtKB
- proteolysis Source: MGI
- regulation of blood pressure Source: MGI
- regulation of blood volume by renin-angiotensin Source: MGI
- regulation of MAPK cascade Source: MGI
- renin-angiotensin regulation of aldosterone production Source: MGI
- response to cAMP Source: MGI
- response to cGMP Source: MGI
- response to immobilization stress Source: Ensembl
- response to lipopolysaccharide Source: Ensembl
- response to organic substance Source: MGI
- response to xenobiotic stimulus Source: MGI
Keywordsi
Molecular function | Aspartyl protease, Hydrolase, Protease |
Enzyme and pathway databases
Reactomei | R-MMU-2022377, Metabolism of Angiotensinogen to Angiotensins |
Protein family/group databases
MEROPSi | A01.008 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ren1 Synonyms:Ren, Ren-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97898, Ren1 |
VEuPathDBi | HostDB:ENSMUSG00000070645 |
Subcellular locationi
Extracellular region or secreted
- extracellular space Source: MGI
Other locations
- apical part of cell Source: MGI
- cytoplasm Source: MGI
- membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Membrane, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | 1 PublicationAdd BLAST | 21 | |
PropeptideiPRO_0000026089 | 22 – 71 | Activation peptide1 PublicationAdd BLAST | 50 | |
ChainiPRO_0000026090 | 72 – 402 | Renin-1Add BLAST | 331 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 69 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 115 ↔ 122 | By similarity | ||
Glycosylationi | 139 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 278 ↔ 282 | By similarity | ||
Glycosylationi | 320 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
jPOSTi | P06281 |
MaxQBi | P06281 |
PaxDbi | P06281 |
PeptideAtlasi | P06281 |
PRIDEi | P06281 |
ProteomicsDBi | 255225 |
PTM databases
GlyGeni | P06281, 3 sites |
PhosphoSitePlusi | P06281 |
Expressioni
Tissue specificityi
Kidney.
Inductioni
Renal renin is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration.
Gene expression databases
Bgeei | ENSMUSG00000070645, Expressed in submandibular gland and 115 other tissues |
Genevisiblei | P06281, MM |
Interactioni
Subunit structurei
Interacts with ATP6AP2.
By similarityGO - Molecular functioni
- insulin-like growth factor receptor binding Source: MGI
- signaling receptor binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000092135 |
Chemistry databases
BindingDBi | P06281 |
Miscellaneous databases
RNActi | P06281, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P06281 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 84 – 399 | Peptidase A1PROSITE-ProRule annotationAdd BLAST | 316 |
Sequence similaritiesi
Belongs to the peptidase A1 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1339, Eukaryota |
GeneTreei | ENSGT00940000157898 |
HOGENOMi | CLU_013253_3_3_1 |
InParanoidi | P06281 |
OMAi | QITMKGV |
OrthoDBi | 1619495at2759 |
PhylomeDBi | P06281 |
TreeFami | TF314990 |
Family and domain databases
CDDi | cd05487, renin_like, 1 hit |
Gene3Di | 2.40.70.10, 2 hits |
InterProi | View protein in InterPro IPR001461, Aspartic_peptidase_A1 IPR001969, Aspartic_peptidase_AS IPR012848, Aspartic_peptidase_N IPR033121, PEPTIDASE_A1 IPR021109, Peptidase_aspartic_dom_sf IPR034135, Renin-like_dom |
PANTHERi | PTHR47966, PTHR47966, 1 hit |
Pfami | View protein in Pfam PF07966, A1_Propeptide, 1 hit PF00026, Asp, 1 hit |
PRINTSi | PR00792, PEPSIN |
SUPFAMi | SSF50630, SSF50630, 1 hit |
PROSITEi | View protein in PROSITE PS00141, ASP_PROTEASE, 2 hits PS51767, PEPTIDASE_A1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P06281-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDRRRMPLWA LLLLWSPCTF SLPTRTATFE RIPLKKMPSV REILEERGVD
60 70 80 90 100
MTRLSAEWGV FTKRPSLTNL TSPVVLTNYL NTQYYGEIGI GTPPQTFKVI
110 120 130 140 150
FDTGSANLWV PSTKCSRLYL ACGIHSLYES SDSSSYMENG SDFTIHYGSG
160 170 180 190 200
RVKGFLSQDS VTVGGITVTQ TFGEVTELPL IPFMLAKFDG VLGMGFPAQA
210 220 230 240 250
VGGVTPVFDH ILSQGVLKEE VFSVYYNRGS HLLGGEVVLG GSDPQHYQGN
260 270 280 290 300
FHYVSISKTD SWQITMKGVS VGSSTLLCEE GCAVVVDTGS SFISAPTSSL
310 320 330 340 350
KLIMQALGAK EKRIEEYVVN CSQVPTLPDI SFDLGGRAYT LSSTDYVLQY
360 370 380 390 400
PNRRDKLCTL ALHAMDIPPP TGPVWVLGAT FIRKFYTEFD RHNNRIGFAL
AR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ9R259 | Q9R259_MOUSE | Renin b | Ren1 | 44 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 – 23 | Missing (PubMed:6370686).CuratedAdd BLAST | 18 | |
Sequence conflicti | 24 | T → I (PubMed:6370686).Curated | 1 | |
Sequence conflicti | 163 | V → VSRV (PubMed:6370686).Curated | 1 |
Polymorphismi
In inbred mouse strains, there are at least two alleles which can occur at the Ren1 locus: Ren-1D and Ren-1C. The sequence shown is that of Ren-1C.
Present as a single-copy gene in strains such as BALB/c and C57BL/6 while some strains such as Swiss and Akr contain two copies.
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 58 | W → R in allele Ren-1D. 1 Publication | 1 | |
Natural varianti | 68 | T → I in allele Ren-1D. 1 Publication | 1 | |
Natural varianti | 160 | S → V in allele Ren-1D; requires 2 nucleotide substitutions. 1 Publication | 1 | |
Natural varianti | 315 | E → D in allele Ren-1D. 2 Publications | 1 | |
Natural varianti | 352 | N → Y in allele Ren-1D. Combined sources1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00810 , X00811, X00812, X00813, X00814, X00815, X00816, X00850, X00851 Genomic DNA Translation: CAA25391.1 X16642 mRNA Translation: CAA34636.1 K02596 Genomic DNA Translation: AAA40045.1 M32352 Genomic DNA Translation: AAA40043.1 AK052685 mRNA Translation: BAC35094.1 AK085309 mRNA Translation: BAC39418.1 BC061053 mRNA Translation: AAH61053.1 K02800 Genomic DNA Translation: AAA40044.1 M34190 Genomic DNA Translation: AAA40042.1 |
CCDSi | CCDS15295.1 |
PIRi | A00989, REMSK |
RefSeqi | NP_112469.1, NM_031192.3 |
Genome annotation databases
Ensembli | ENSMUST00000094556; ENSMUSP00000092135; ENSMUSG00000070645 |
GeneIDi | 19701 |
KEGGi | mmu:19701 |
UCSCi | uc007cqf.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00810 , X00811, X00812, X00813, X00814, X00815, X00816, X00850, X00851 Genomic DNA Translation: CAA25391.1 X16642 mRNA Translation: CAA34636.1 K02596 Genomic DNA Translation: AAA40045.1 M32352 Genomic DNA Translation: AAA40043.1 AK052685 mRNA Translation: BAC35094.1 AK085309 mRNA Translation: BAC39418.1 BC061053 mRNA Translation: AAH61053.1 K02800 Genomic DNA Translation: AAA40044.1 M34190 Genomic DNA Translation: AAA40042.1 |
CCDSi | CCDS15295.1 |
PIRi | A00989, REMSK |
RefSeqi | NP_112469.1, NM_031192.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5MKT | X-ray | 3.20 | A | 22-402 | [»] | |
SMRi | P06281 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000092135 |
Chemistry databases
BindingDBi | P06281 |
ChEMBLi | CHEMBL2615 |
DrugCentrali | P06281 |
Protein family/group databases
MEROPSi | A01.008 |
PTM databases
GlyGeni | P06281, 3 sites |
PhosphoSitePlusi | P06281 |
Proteomic databases
jPOSTi | P06281 |
MaxQBi | P06281 |
PaxDbi | P06281 |
PeptideAtlasi | P06281 |
PRIDEi | P06281 |
ProteomicsDBi | 255225 |
Protocols and materials databases
Antibodypediai | 1026, 764 antibodies from 40 providers |
DNASUi | 19701 |
Genome annotation databases
Ensembli | ENSMUST00000094556; ENSMUSP00000092135; ENSMUSG00000070645 |
GeneIDi | 19701 |
KEGGi | mmu:19701 |
UCSCi | uc007cqf.1, mouse |
Organism-specific databases
CTDi | 19701 |
MGIi | MGI:97898, Ren1 |
VEuPathDBi | HostDB:ENSMUSG00000070645 |
Phylogenomic databases
eggNOGi | KOG1339, Eukaryota |
GeneTreei | ENSGT00940000157898 |
HOGENOMi | CLU_013253_3_3_1 |
InParanoidi | P06281 |
OMAi | QITMKGV |
OrthoDBi | 1619495at2759 |
PhylomeDBi | P06281 |
TreeFami | TF314990 |
Enzyme and pathway databases
Reactomei | R-MMU-2022377, Metabolism of Angiotensinogen to Angiotensins |
Miscellaneous databases
BioGRID-ORCSi | 19701, 1 hit in 65 CRISPR screens |
ChiTaRSi | Ren1, mouse |
PROi | PR:P06281 |
RNActi | P06281, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000070645, Expressed in submandibular gland and 115 other tissues |
Genevisiblei | P06281, MM |
Family and domain databases
CDDi | cd05487, renin_like, 1 hit |
Gene3Di | 2.40.70.10, 2 hits |
InterProi | View protein in InterPro IPR001461, Aspartic_peptidase_A1 IPR001969, Aspartic_peptidase_AS IPR012848, Aspartic_peptidase_N IPR033121, PEPTIDASE_A1 IPR021109, Peptidase_aspartic_dom_sf IPR034135, Renin-like_dom |
PANTHERi | PTHR47966, PTHR47966, 1 hit |
Pfami | View protein in Pfam PF07966, A1_Propeptide, 1 hit PF00026, Asp, 1 hit |
PRINTSi | PR00792, PEPSIN |
SUPFAMi | SSF50630, SSF50630, 1 hit |
PROSITEi | View protein in PROSITE PS00141, ASP_PROTEASE, 2 hits PS51767, PEPTIDASE_A1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RENI1_MOUSE | |
Accessioni | P06281Primary (citable) accession number: P06281 Secondary accession number(s): P97911 Q62154 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | February 23, 2022 | |
This is version 192 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families