UniProtKB - P06245 (KAPB_YEAST)
Protein
cAMP-dependent protein kinase type 2
Gene
TPK2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 2220 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Activity regulationi
Activated by cAMP.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 99 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 193 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 76 – 84 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- AMP-activated protein kinase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- cAMP-dependent protein kinase activity Source: SGD
- protein kinase activity Source: SGD
- translation initiation factor binding Source: SGD
GO - Biological processi
- invasive growth in response to glucose limitation Source: SGD
- negative regulation of cytoplasmic mRNA processing body assembly Source: SGD
- negative regulation of cytoplasmic translation Source: SGD
- protein kinase A signaling Source: SGD
- protein phosphorylation Source: SGD
- Ras protein signal transduction Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, cAMP, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.11, 984 |
Reactomei | R-SCE-163615, PKA activation R-SCE-164378, PKA activation in glucagon signalling R-SCE-180024, DARPP-32 events R-SCE-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-SCE-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-SCE-5610787, Hedgehog 'off' state R-SCE-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:TPK2 Synonyms:PKA2, YKR1 Ordered Locus Names:YPL203W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006124, TPK2 |
VEuPathDBi | FungiDB:YPL203W |
Subcellular locationi
Cytosol
- cytosol Source: SGD
Nucleus
- nuclear chromatin Source: SGD
- nucleus Source: SGD
Other locations
- cAMP-dependent protein kinase complex Source: SGD
- P-body Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086048 | 1 – 380 | cAMP-dependent protein kinase type 2Add BLAST | 380 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 224 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P06245 |
PaxDbi | P06245 |
PRIDEi | P06245 |
PTM databases
iPTMneti | P06245 |
Interactioni
Binary interactionsi
P06245
With | #Exp. | IntAct |
---|---|---|
BCY1 [P07278] | 6 | EBI-9465,EBI-9475 |
GO - Molecular functioni
- translation initiation factor binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 35981, 216 interactors |
ComplexPortali | CPX-537, cAMP-dependent protein kinase complex variant 2 CPX-572, cAMP-dependent protein kinase complex variant 4 CPX-574, cAMP-dependent protein kinase complex variant 6 |
DIPi | DIP-549N |
IntActi | P06245, 46 interactors |
MINTi | P06245 |
STRINGi | 4932.YPL203W |
Miscellaneous databases
RNActi | P06245, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 70 – 324 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 255 | |
Domaini | 325 – 380 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 56 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0616, Eukaryota |
GeneTreei | ENSGT00940000176357 |
HOGENOMi | CLU_000288_63_5_1 |
InParanoidi | P06245 |
OMAi | SSLFDHY |
Family and domain databases
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P06245-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEFVAERAQP VGQTIQQQNV NTYGQGVLQP HHDLQQRQQQ QQQRQHQQLL
60 70 80 90 100
TSQLPQKSLV SKGKYTLHDF QIMRTLGTGS FGRVHLVRSV HNGRYYAIKV
110 120 130 140 150
LKKQQVVKMK QVEHTNDERR MLKLVEHPFL IRMWGTFQDA RNIFMVMDYI
160 170 180 190 200
EGGELFSLLR KSQRFPNPVA KFYAAEVILA LEYLHAHNII YRDLKPENIL
210 220 230 240 250
LDRNGHIKIT DFGFAKEVQT VTWTLCGTPD YIAPEVITTK PYNKSVDWWS
260 270 280 290 300
LGVLIYEMLA GYTPFYDTTP MKTYEKILQG KVVYPPYFHP DVVDLLSKLI
310 320 330 340 350
TADLTRRIGN LQSGSRDIKA HPWFSEVVWE RLLAKDIETP YEPPITSGIG
360 370 380
DTSLFDQYPE EQLDYGIQGD DPYAEYFQDF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 214 – 220 | FAKEVQT → LRQRGTN in CAA68689 (PubMed:3308514).Curated | 7 | |
Sequence conflicti | 260 | A → V in CAA68689 (PubMed:3308514).Curated | 1 | |
Sequence conflicti | 289 | H → Q in AAA35165 (PubMed:3036373).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17073 Genomic DNA Translation: AAA35165.1 Y00694 Genomic DNA Translation: CAA68689.1 Z73559 Genomic DNA Translation: CAA97917.1 BK006949 Genomic DNA Translation: DAA11232.1 |
PIRi | S65222, OKBYC2 |
RefSeqi | NP_015121.1, NM_001184017.1 |
Genome annotation databases
EnsemblFungii | YPL203W_mRNA; YPL203W; YPL203W |
GeneIDi | 855898 |
KEGGi | sce:YPL203W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17073 Genomic DNA Translation: AAA35165.1 Y00694 Genomic DNA Translation: CAA68689.1 Z73559 Genomic DNA Translation: CAA97917.1 BK006949 Genomic DNA Translation: DAA11232.1 |
PIRi | S65222, OKBYC2 |
RefSeqi | NP_015121.1, NM_001184017.1 |
3D structure databases
SMRi | P06245 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35981, 216 interactors |
ComplexPortali | CPX-537, cAMP-dependent protein kinase complex variant 2 CPX-572, cAMP-dependent protein kinase complex variant 4 CPX-574, cAMP-dependent protein kinase complex variant 6 |
DIPi | DIP-549N |
IntActi | P06245, 46 interactors |
MINTi | P06245 |
STRINGi | 4932.YPL203W |
PTM databases
iPTMneti | P06245 |
Proteomic databases
MaxQBi | P06245 |
PaxDbi | P06245 |
PRIDEi | P06245 |
Genome annotation databases
EnsemblFungii | YPL203W_mRNA; YPL203W; YPL203W |
GeneIDi | 855898 |
KEGGi | sce:YPL203W |
Organism-specific databases
SGDi | S000006124, TPK2 |
VEuPathDBi | FungiDB:YPL203W |
Phylogenomic databases
eggNOGi | KOG0616, Eukaryota |
GeneTreei | ENSGT00940000176357 |
HOGENOMi | CLU_000288_63_5_1 |
InParanoidi | P06245 |
OMAi | SSLFDHY |
Enzyme and pathway databases
BRENDAi | 2.7.11.11, 984 |
Reactomei | R-SCE-163615, PKA activation R-SCE-164378, PKA activation in glucagon signalling R-SCE-180024, DARPP-32 events R-SCE-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-SCE-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-SCE-5610787, Hedgehog 'off' state R-SCE-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
PROi | PR:P06245 |
RNActi | P06245, protein |
Family and domain databases
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KAPB_YEAST | |
Accessioni | P06245Primary (citable) accession number: P06245 Secondary accession number(s): D6W3G6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 205 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - SIMILARITY comments
Index of protein domains and families