UniProtKB - P06244 (KAPA_YEAST)
Protein
cAMP-dependent protein kinase type 1
Gene
TPK1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 4320 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Activity regulationi
Activated by cAMP.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 116 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 210 | Proton acceptor | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 93 – 101 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- AMP-activated protein kinase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- cAMP-dependent protein kinase activity Source: SGD
- protein kinase activity Source: SGD
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- negative regulation of cytoplasmic mRNA processing body assembly Source: SGD
- peptidyl-serine phosphorylation Source: SGD
- protein kinase A signaling Source: SGD
- protein phosphorylation Source: SGD
- Ras protein signal transduction Source: SGD
- regulation of macroautophagy Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division |
Ligand | ATP-binding, cAMP, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.11, 984 |
Reactomei | R-SCE-163615, PKA activation R-SCE-164378, PKA activation in glucagon signalling R-SCE-180024, DARPP-32 events R-SCE-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-SCE-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-SCE-5610787, Hedgehog 'off' state R-SCE-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | Recommended name: cAMP-dependent protein kinase type 1 (EC:2.7.11.11)Short name: PKA 1 Alternative name(s): CDC25-suppressing protein kinase PK-25 |
Gene namesi | Name:TPK1 Synonyms:PKA1, SRA3 Ordered Locus Names:YJL164C ORF Names:J0541 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003700, TPK1 |
VEuPathDBi | FungiDB:YJL164C |
Subcellular locationi
Nucleus
- nuclear chromatin Source: SGD
- nucleus Source: SGD
Other locations
- cAMP-dependent protein kinase complex Source: SGD
- cytoplasm Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000086046 | 2 – 397 | cAMP-dependent protein kinase type 1Add BLAST | 396 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | P06244 |
PaxDbi | P06244 |
PRIDEi | P06244 |
PTM databases
iPTMneti | P06244 |
Interactioni
Binary interactionsi
Hide detailsP06244
With | #Exp. | IntAct |
---|---|---|
BCY1 [P07278] | 7 | EBI-9458,EBI-9475 |
GPB2 [P39717] | 3 | EBI-9458,EBI-20711 |
Protein-protein interaction databases
BioGRIDi | 33595, 435 interactors |
ComplexPortali | CPX-536, cAMP-dependent protein kinase complex variant 1 CPX-572, cAMP-dependent protein kinase complex variant 4 CPX-573, cAMP-dependent protein kinase complex variant 5 |
DIPi | DIP-548N |
ELMi | P06244 |
IntActi | P06244, 28 interactors |
MINTi | P06244 |
STRINGi | 4932.YJL164C |
Miscellaneous databases
RNActi | P06244, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P06244 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P06244 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 87 – 341 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 255 | |
Domaini | 342 – 397 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 56 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0616, Eukaryota |
GeneTreei | ENSGT00940000176357 |
HOGENOMi | CLU_000288_63_5_1 |
InParanoidi | P06244 |
OMAi | FWTNHDE |
Family and domain databases
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P06244-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTEEQNGGG QKSLDDRQGE ESQKGETSER ETTATESGNE SKSVEKEGGE
60 70 80 90 100
TQEKPKQPHV TYYNEEQYKQ FIAQARVTSG KYSLQDFQIL RTLGTGSFGR
110 120 130 140 150
VHLIRSRHNG RYYAMKVLKK EIVVRLKQVE HTNDERLMLS IVTHPFIIRM
160 170 180 190 200
WGTFQDAQQI FMIMDYIEGG ELFSLLRKSQ RFPNPVAKFY AAEVCLALEY
210 220 230 240 250
LHSKDIIYRD LKPENILLDK NGHIKITDFG FAKYVPDVTY TLCGTPDYIA
260 270 280 290 300
PEVVSTKPYN KSIDWWSFGI LIYEMLAGYT PFYDSNTMKT YEKILNAELR
310 320 330 340 350
FPPFFNEDVK DLLSRLITRD LSQRLGNLQN GTEDVKNHPW FKEVVWEKLL
360 370 380 390
SRNIETPYEP PIQQGQGDTS QFDKYPEEDI NYGVQGEDPY ADLFRDF
Sequence cautioni
The sequence AAA35088 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 79 – 86 | SGKYSLQD → VGSIVYKN in AAA35088 (PubMed:2823100).Curated | 8 | |
Sequence conflicti | 79 – 86 | SGKYSLQD → VGSIVYKN in CAA89459 (PubMed:8641269).Curated | 8 | |
Sequence conflicti | 164 – 171 | MDYIEGGE → ILKVER in AAA35088 (PubMed:2823100).Curated | 8 | |
Sequence conflicti | 180 – 181 | QR → KD in AAA35088 (PubMed:2823100).Curated | 2 | |
Sequence conflicti | 188 – 190 | KFY → QIF in AAA35088 (PubMed:2823100).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17072 Genomic DNA Translation: AAA35164.1 J02665 Genomic DNA Translation: AAA34877.1 M17224 Genomic DNA Translation: AAA35088.1 Different initiation. Z49439 Genomic DNA Translation: CAA89459.1 AY899257 mRNA Translation: AAX83942.1 BK006943 Genomic DNA Translation: DAA08639.2 |
PIRi | JC1034, OKBYC1 |
RefSeqi | NP_012371.2, NM_001181597.2 |
Genome annotation databases
EnsemblFungii | YJL164C_mRNA; YJL164C; YJL164C |
GeneIDi | 853275 |
KEGGi | sce:YJL164C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17072 Genomic DNA Translation: AAA35164.1 J02665 Genomic DNA Translation: AAA34877.1 M17224 Genomic DNA Translation: AAA35088.1 Different initiation. Z49439 Genomic DNA Translation: CAA89459.1 AY899257 mRNA Translation: AAX83942.1 BK006943 Genomic DNA Translation: DAA08639.2 |
PIRi | JC1034, OKBYC1 |
RefSeqi | NP_012371.2, NM_001181597.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FOT | X-ray | 2.80 | A | 81-397 | [»] | |
SMRi | P06244 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33595, 435 interactors |
ComplexPortali | CPX-536, cAMP-dependent protein kinase complex variant 1 CPX-572, cAMP-dependent protein kinase complex variant 4 CPX-573, cAMP-dependent protein kinase complex variant 5 |
DIPi | DIP-548N |
ELMi | P06244 |
IntActi | P06244, 28 interactors |
MINTi | P06244 |
STRINGi | 4932.YJL164C |
PTM databases
iPTMneti | P06244 |
Proteomic databases
MaxQBi | P06244 |
PaxDbi | P06244 |
PRIDEi | P06244 |
Genome annotation databases
EnsemblFungii | YJL164C_mRNA; YJL164C; YJL164C |
GeneIDi | 853275 |
KEGGi | sce:YJL164C |
Organism-specific databases
SGDi | S000003700, TPK1 |
VEuPathDBi | FungiDB:YJL164C |
Phylogenomic databases
eggNOGi | KOG0616, Eukaryota |
GeneTreei | ENSGT00940000176357 |
HOGENOMi | CLU_000288_63_5_1 |
InParanoidi | P06244 |
OMAi | FWTNHDE |
Enzyme and pathway databases
BRENDAi | 2.7.11.11, 984 |
Reactomei | R-SCE-163615, PKA activation R-SCE-164378, PKA activation in glucagon signalling R-SCE-180024, DARPP-32 events R-SCE-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-SCE-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-SCE-5610787, Hedgehog 'off' state R-SCE-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
EvolutionaryTracei | P06244 |
PROi | PR:P06244 |
RNActi | P06244, protein |
Family and domain databases
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KAPA_YEAST | |
Accessioni | P06244Primary (citable) accession number: P06244 Secondary accession number(s): D6VW23, P11595, Q2VQW1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | September 21, 2011 | |
Last modified: | February 10, 2021 | |
This is version 216 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families