Lck

Functionⁱ

Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP (By similarity). Interacts with UNC119; this interaction plays a crucial role in activation of LCK (By similarity).By similarity

Catalytic activityⁱ

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Activity regulationⁱ

The relative activities of the inhibitory tyrosine-protein kinase CSK and the activating tyrosine-protein phosphatase PTPRC/CD45 determine the level of LCK activity. These interactions allow rapid and efficient activation of LCK in response to TCR stimulation (By similarity).By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	273	ATPPROSITE-ProRule annotation		1
Active siteⁱ	364	Proton acceptorPROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	251 – 259	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

antigen binding Source: MGI
ATPase binding Source: MGI
ATP binding Source: UniProtKB-KW
CD4 receptor binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 3262426
CD8 receptor binding Source: MGI
identical protein binding Source: MGI
non-membrane spanning protein tyrosine kinase activity Source: GO_Central
phosphatidylinositol 3-kinase binding Source: MGI
phosphotyrosine residue binding Source: MGI
protein antigen binding
Source: MGI
Inferred from physical interactionⁱ
- 21245484
protein-containing complex binding Source: MGI
protein C-terminus binding Source: MGI
protein kinase binding Source: MGI
protein phosphatase binding Source: MGI
protein serine/threonine phosphatase activity Source: UniProtKB
protein tyrosine kinase activity
Source: MGI
Inferred from direct assayⁱ
SH2 domain binding Source: UniProtKB
T cell receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
B cell receptor signaling pathway
Source: MGI
Inferred from direct assayⁱ
- 7499277
cell migration Source: GO_Central
cell surface receptor signaling pathway
Source: MGI
Inferred from direct assayⁱ
- 9177270
cellular zinc ion homeostasis Source: UniProtKB
innate immune response Source: GO_Central
peptidyl-tyrosine autophosphorylation Source: GO_Central
peptidyl-tyrosine phosphorylation
Source: MGI
Inferred from direct assayⁱ
- 7499277
- 8175795
positive regulation of gamma-delta T cell differentiation
Source: MGI
Inferred from mutant phenotypeⁱ
- 7807014
positive regulation of gene expression
Source: MGI
Inferred from direct assayⁱ
- 21357543
positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
positive regulation of T cell activation Source: UniProtKB
positive regulation of tyrosine phosphorylation of STAT protein
Source: MGI
Inferred from direct assayⁱ
- 11254359
positive regulation of uterine smooth muscle contraction Source: MGI
protein autophosphorylation
Source: MGI
Inferred from direct assayⁱ
- 8175795
protein phosphorylation
Source: MGI
Inferred from direct assayⁱ
- 9169414
regulation of apoptotic process Source: GO_Central
regulation of cell proliferation Source: GO_Central
regulation of regulatory T cell differentiation
Source: MGI
Inferred from genetic interactionⁱ
- 21245484
regulation of T cell receptor signaling pathway
Source: MGI
Inferred from direct assayⁱ
- 14752163
release of sequestered calcium ion into cytosol
Source: UniProtKB
Inferred from direct assayⁱ
- 1505025
response to drug Source: UniProtKB
response to hydrogen peroxide Source: MGI
response to mechanical stimulus Source: MGI
response to metal ion Source: MGI
T cell differentiation Source: UniProtKB
transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Transferase, Tyrosine-protein kinase
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAⁱ	2.7.10.2 3474
Reactomeⁱ	R-MMU-114604 GPVI-mediated activation cascade R-MMU-1257604 PIP3 activates AKT signaling R-MMU-1433557 Signaling by SCF-KIT R-MMU-1433559 Regulation of KIT signaling R-MMU-202424 Downstream TCR signaling R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains R-MMU-202430 Translocation of ZAP-70 to Immunological synapse R-MMU-202433 Generation of second messenger molecules R-MMU-2424491 DAP12 signaling R-MMU-389356 CD28 co-stimulation R-MMU-389357 CD28 dependent PI3K/Akt signaling R-MMU-389359 CD28 dependent Vav1 pathway R-MMU-389513 CTLA4 inhibitory signaling R-MMU-389948 PD-1 signaling R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-MMU-9020558 Interleukin-2 signaling

BRENDAⁱ

2.7.10.2 3474

Reactomeⁱ

R-MMU-114604 GPVI-mediated activation cascade
R-MMU-1257604 PIP3 activates AKT signaling
R-MMU-1433557 Signaling by SCF-KIT
R-MMU-1433559 Regulation of KIT signaling
R-MMU-202424 Downstream TCR signaling
R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains
R-MMU-202430 Translocation of ZAP-70 to Immunological synapse
R-MMU-202433 Generation of second messenger molecules
R-MMU-2424491 DAP12 signaling
R-MMU-389356 CD28 co-stimulation
R-MMU-389357 CD28 dependent PI3K/Akt signaling
R-MMU-389359 CD28 dependent Vav1 pathway
R-MMU-389513 CTLA4 inhibitory signaling
R-MMU-389948 PD-1 signaling
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-MMU-9020558 Interleukin-2 signaling

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Proto-oncogene tyrosine-protein kinase LCK (EC:2.7.10.2) Alternative name(s): Leukocyte C-terminal Src kinase Short name: LSK Lymphocyte cell-specific protein-tyrosine kinase p56-LCK
Gene namesⁱ	Name:Lck Synonyms:Lsk-t
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 4

Organism-specific databases

MGIⁱ	MGI:96756 Lck

Keywords - Cellular componentⁱ

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Mice show a dramatic reduction in the level of peripheral T-cells, with 5-10% of wild-type levels. T-cells also exhibit a 10-fold decrease in proliferative response.1 Publication

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	2	G → A: Abolishes myristoylation and palmitoylation. 1 Publication	1
Mutagenesisⁱ	3 – 5	CVC → SVK: Complete loss of interaction with CD4 or CD8. 1 Publication	3
Mutagenesisⁱ	3	C → S: Abolishes plasma membrane association; when associated with S-41. Reduced palmitoylation level. 2 Publications	1
Mutagenesisⁱ	5	C → K: Reduced palmitoylation level. 2 Publications	1
Mutagenesisⁱ	5	C → S: Abolishes plasma membrane association; when associated with S-21. 2 Publications	1
Mutagenesisⁱ	20	C → S: Complete loss of interaction with CD4 or CD8. 1 Publication	1
Mutagenesisⁱ	23	C → S: Complete loss of interaction with CD4 or CD8. 1 Publication	1
Mutagenesisⁱ	269	K → N: Reduced activity.	1
Mutagenesisⁱ	270	V → L: Reduced activity.	1
Mutagenesisⁱ	271	A → S: Reduced activity.	1
Mutagenesisⁱ	272	V → A: Reduced activity.	1
Mutagenesisⁱ	273	K → R: Loss of activity. 1 Publication	1
Mutagenesisⁱ	274	S → N: Reduced activity.	1
Mutagenesisⁱ	275	L → M: Reduced activity.	1
Mutagenesisⁱ	276	K → V: Reduced activity.	1
Mutagenesisⁱ	505	Y → F: Causes thymic tumors. 2 Publications	1

Keywords - Diseaseⁱ

Proto-oncogene

Chemistry databases

ChEMBLⁱ	CHEMBL2480

ChEMBLⁱ

CHEMBL2480

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCurated
ChainⁱPRO_0000088125	2 – 509	Proto-oncogene tyrosine-protein kinase LCKAdd BLAST		508

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Lipidationⁱ	2	N-myristoyl glycine1 Publication	1
Lipidationⁱ	3	S-palmitoyl cysteine2 Publications	1
Lipidationⁱ	5	S-palmitoyl cysteine2 Publications	1
Modified residueⁱ	102	PhosphoserineBy similarity	1
Modified residueⁱ	159	PhosphothreonineBy similarity	1
Modified residueⁱ	162	PhosphoserineBy similarity	1
Modified residueⁱ	192	PhosphotyrosineCombined sources	1
Modified residueⁱ	194	PhosphoserineBy similarity	1
Modified residueⁱ	394	Phosphotyrosine; by autocatalysisBy similarity	1
Modified residueⁱ	505	Phosphotyrosine; by CSKBy similarity	1

Post-translational modificationⁱ

Autophosphorylated on Tyr-394, increasing enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-505 by CSK, decreasing activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-394 by PTPN2 negatively regulates T-cells differentiation (By similarity).By similarity

Myristoylation is required prior to palmitoylation.2 Publications

Palmitoylation regulates subcellular location.By similarity

Keywords - PTMⁱ

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

EPDⁱ	P06240
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P06240
PaxDbⁱ	P06240
PRIDEⁱ	P06240

PTM databases

iPTMnetⁱ	P06240
PhosphoSitePlusⁱ	P06240
SwissPalmⁱ	P06240

Expressionⁱ

Tissue specificityⁱ

Present at a low level in most T-cells, and at an elevated level in LSTRA and Thy19 (T-cell lymphoma) cells.

Developmental stageⁱ

Levels remain relatively constant throughout T-cell ontogeny.

Gene expression databases

Bgeeⁱ	ENSMUSG00000000409 Expressed in 161 organ(s), highest expression level in thymus
CleanExⁱ	MM_LCK
ExpressionAtlasⁱ	P06240 baseline and differential
Genevisibleⁱ	P06240 MM

Interactionⁱ

Subunit structureⁱ

Binds to the cytoplasmic domain of cell surface receptors, such as AXL, CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB1 and to other protein kinases including CDK1, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. Interacts with SQSTM1. Interacts with phosphorylated LIME1. Interacts with CBLB and PTPRH. Interacts with RUNX3. Forms a signaling complex with EPHA1, PTK2B AND PI3-KINASE; upon activation by EFNA1 which may regulate T-lymphocytes migration. Associates with ZAP70 and RHOH; these interactions allow LCK-mediated RHOH and CD3 subunit phosphorylation in the presence of functional ZAP70. Interacts with CEACAM1 (via cytoplasmic domain); mediates CEACAM1 phosphorylation resulting in PTPN6 recruitment that dephosphorylates TCR stimulation-induced CD247 and ZAP70. Interacts with FYB2 (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
	O92972	2	EBI-1401,EBI-710506	From Hepatitis C virus genotype 1b (strain HC-J4).
	P27958	3	EBI-1401,EBI-706378	From Hepatitis C virus genotype 1a (isolate H).
Cd4	P06332	3	EBI-1401,EBI-1404
Cd8a	P01731	2	EBI-1401,EBI-1433
KHDRBS1	Q07666	2	EBI-1401,EBI-1364	From Homo sapiens.
PTPRC	P08575	2	EBI-1401,EBI-1341	From Homo sapiens.
Ptprc	P06800	2	EBI-1401,EBI-1672
Sh2d2a	Q9QXK9	3	EBI-1401,EBI-1644

GO - Molecular functionⁱ

ATPase binding Source: MGI
CD4 receptor binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 3262426
CD8 receptor binding Source: MGI
identical protein binding Source: MGI
phosphatidylinositol 3-kinase binding Source: MGI
phosphotyrosine residue binding Source: MGI
protein antigen binding
Source: MGI
Inferred from physical interactionⁱ
- 21245484
protein C-terminus binding Source: MGI
protein kinase binding Source: MGI
protein phosphatase binding Source: MGI
SH2 domain binding Source: UniProtKB
T cell receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	201120, 28 interactors
CORUMⁱ	P06240
ELMⁱ	P06240
IntActⁱ	P06240, 33 interactors
Molecular INTeraction database More...MINTⁱ	P06240
STRINGⁱ	10090.ENSMUSP00000125777

Chemistry databases

BindingDBⁱ

P06240

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P06240
SMRⁱ	P06240
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	61 – 121	SH3PROSITE-ProRule annotationAdd BLAST	61
Domainⁱ	127 – 224	SH2PROSITE-ProRule annotationAdd BLAST	98
Domainⁱ	245 – 498	Protein kinasePROSITE-ProRule annotationAdd BLAST	254

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	2 – 72	Interactions with CD4 and CD8Add BLAST		71
Regionⁱ	154 – 242	Interaction with PTPRHBy similarityAdd BLAST		89

Domainⁱ

The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation (By similarity).By similarity

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation

Keywords - Domainⁱ

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGⁱ	KOG0197 Eukaryota COG0515 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118938
HOGENOMⁱ	HOG000233858
HOVERGENⁱ	HBG008761
InParanoidⁱ	P06240
KEGG Orthology (KO) More...KOⁱ	K05856
PhylomeDBⁱ	P06240
TreeFamⁱ	TF351634

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd10362 SH2_Src_Lck, 1 hit cd12005 SH3_Lck, 1 hit
Gene3Dⁱ	3.30.505.10, 1 hit
InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR035850 Lck_SH2 IPR035749 Lck_SH3 IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR000980 SH2 IPR036860 SH2_dom_sf IPR036028 SH3-like_dom_sf IPR001452 SH3_domain IPR008266 Tyr_kinase_AS IPR020635 Tyr_kinase_cat_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07714 Pkinase_Tyr, 1 hit PF00017 SH2, 1 hit PF00018 SH3_1, 1 hit
PRINTSⁱ	PR00401 SH2DOMAIN PR00452 SH3DOMAIN PR00109 TYRKINASE
SMARTⁱ	View protein in SMART SM00252 SH2, 1 hit SM00326 SH3, 1 hit SM00219 TyrKc, 1 hit
SUPFAMⁱ	SSF50044 SSF50044, 1 hit SSF55550 SSF55550, 1 hit SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00109 PROTEIN_KINASE_TYR, 1 hit PS50001 SH2, 1 hit PS50002 SH3, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show all Align All

P06240-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGCVCSSNPE DDWMENIDVC ENCHYPIVPL DSKISLPIRN GSEVRDPLVT 
        60         70         80         90        100
YEGSLPPASP LQDNLVIALH SYEPSHDGDL GFEKGEQLRI LEQSGEWWKA 
       110        120        130        140        150
QSLTTGQEGF IPFNFVAKAN SLEPEPWFFK NLSRKDAERQ LLAPGNTHGS 
       160        170        180        190        200
FLIRESESTA GSFSLSVRDF DQNQGEVVKH YKIRNLDNGG FYISPRITFP 
       210        220        230        240        250
GLHDLVRHYT NASDGLCTKL SRPCQTQKPQ KPWWEDEWEV PRETLKLVER 
       260        270        280        290        300
LGAGQFGEVW MGYYNGHTKV AVKSLKQGSM SPDAFLAEAN LMKQLQHPRL 
       310        320        330        340        350
VRLYAVVTQE PIYIITEYME NGSLVDFLKT PSGIKLNVNK LLDMAAQIAE 
       360        370        380        390        400
GMAFIEEQNY IHRDLRAANI LVSDTLSCKI ADFGLARLIE DNEYTAREGA 
       410        420        430        440        450
KFPIKWTAPE AINYGTFTIK SDVWSFGILL TEIVTHGRIP YPGMTNPEVI 
       460        470        480        490        500
QNLERGYRMV RPDNCPEELY HLMMLCWKER PEDRPTFDYL RSVLDDFFTA 

TEGQYQPQP

Length:509

Mass (Da):57,943

Last modified:January 23, 2007 - v4

Checksum:ⁱ3513102F49A7FD0B

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

E9Q696	E9Q696_MOUSE	Tyrosine-protein kinase Tyrosine-protein kinase, EC 2.7.10.2	Lck	520	Annotation score:
B2KG65	B2KG65_MOUSE	Proto-oncogene tyrosine-protein kin... Proto-oncogene tyrosine-protein kinase LCK	Lck	92	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M12056 mRNA Translation: AAB59674.1 X03533 mRNA Translation: CAA27234.1 X03533 mRNA Translation: CAA27235.1 Sequence problems. X03533 mRNA Translation: CAA27236.1 Sequence problems. AK088001 mRNA Translation: BAC40086.1 BC011474 mRNA Translation: AAH11474.1 M21511 Genomic DNA Translation: AAA39422.1 Sequence problems. M18098 Genomic DNA Translation: AAA39421.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS18697.1
PIRⁱ	I48845
NCBI Reference Sequences More...RefSeqⁱ	NP_001155905.1, NM_001162433.1 NP_034823.1, NM_010693.3 XP_006502881.1, XM_006502818.3
UniGeneⁱ	Mm.293753

Genome annotation databases

Ensemblⁱ	ENSMUST00000067240; ENSMUSP00000066209; ENSMUSG00000000409 ENSMUST00000102596; ENSMUSP00000099656; ENSMUSG00000000409
GeneIDⁱ	16818
KEGGⁱ	mmu:16818
UCSC genome browser More...UCSCⁱ	uc008uxi.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P06240	Tyrosine-protein kinase		MOUSE		520	UniRef100_P06240
Proto-oncogene tyrosine-protein kinase LCK (Fragment)		MOUSE		92

Protein	Similar proteins		Species	Score	Length	Source
P06240	Tyrosine-protein kinase Lck	)	HUMAN		509	UniRef90_P06239
Tyrosine-protein kinase Lck		AOTNA		509
Proto-oncogene tyrosine-protein kinase LCK		SAISC		509
Proto-oncogene tyrosine-protein kinase LCK		RAT		509
Tyrosine-protein kinase		PANTR		527
+146

Protein	Similar proteins		Species	Score	Length	Source
P06240	Tyrosine-protein kinase Fyn	)	HUMAN		537	UniRef50_P06241
Tyrosine-protein kinase fynb		DANRE		544
Tyrosine-protein kinase Fyn		RAT		537
Tyrosine-protein kinase Fyn		BOVIN		537
Tyrosine-protein kinase Fyn	)	MOUSE		537
+797

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M12056 mRNA Translation: AAB59674.1 X03533 mRNA Translation: CAA27234.1 X03533 mRNA Translation: CAA27235.1 Sequence problems. X03533 mRNA Translation: CAA27236.1 Sequence problems. AK088001 mRNA Translation: BAC40086.1 BC011474 mRNA Translation: AAH11474.1 M21511 Genomic DNA Translation: AAA39422.1 Sequence problems. M18098 Genomic DNA Translation: AAA39421.1
CCDSⁱ	CCDS18697.1
PIRⁱ	I48845
RefSeqⁱ	NP_001155905.1, NM_001162433.1 NP_034823.1, NM_010693.3 XP_006502881.1, XM_006502818.3
UniGeneⁱ	Mm.293753

3D structure databases

ProteinModelPortalⁱ	P06240
SMRⁱ	P06240
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	201120, 28 interactors
CORUMⁱ	P06240
ELMⁱ	P06240
IntActⁱ	P06240, 33 interactors
MINTⁱ	P06240
STRINGⁱ	10090.ENSMUSP00000125777

Chemistry databases

BindingDBⁱ	P06240
ChEMBLⁱ	CHEMBL2480

PTM databases

iPTMnetⁱ	P06240
PhosphoSitePlusⁱ	P06240
SwissPalmⁱ	P06240

Proteomic databases

EPDⁱ	P06240
MaxQBⁱ	P06240
PaxDbⁱ	P06240
PRIDEⁱ	P06240

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000067240; ENSMUSP00000066209; ENSMUSG00000000409 ENSMUST00000102596; ENSMUSP00000099656; ENSMUSG00000000409
GeneIDⁱ	16818
KEGGⁱ	mmu:16818
UCSCⁱ	uc008uxi.2 mouse

Organism-specific databases

CTDⁱ	3932
MGIⁱ	MGI:96756 Lck

Phylogenomic databases

eggNOGⁱ	KOG0197 Eukaryota COG0515 LUCA
GeneTreeⁱ	ENSGT00760000118938
HOGENOMⁱ	HOG000233858
HOVERGENⁱ	HBG008761
InParanoidⁱ	P06240
KOⁱ	K05856
PhylomeDBⁱ	P06240
TreeFamⁱ	TF351634

Enzyme and pathway databases

BRENDAⁱ	2.7.10.2 3474
Reactomeⁱ	R-MMU-114604 GPVI-mediated activation cascade R-MMU-1257604 PIP3 activates AKT signaling R-MMU-1433557 Signaling by SCF-KIT R-MMU-1433559 Regulation of KIT signaling R-MMU-202424 Downstream TCR signaling R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains R-MMU-202430 Translocation of ZAP-70 to Immunological synapse R-MMU-202433 Generation of second messenger molecules R-MMU-2424491 DAP12 signaling R-MMU-389356 CD28 co-stimulation R-MMU-389357 CD28 dependent PI3K/Akt signaling R-MMU-389359 CD28 dependent Vav1 pathway R-MMU-389513 CTLA4 inhibitory signaling R-MMU-389948 PD-1 signaling R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-MMU-9020558 Interleukin-2 signaling

BRENDAⁱ

2.7.10.2 3474

Reactomeⁱ

R-MMU-114604 GPVI-mediated activation cascade
R-MMU-1257604 PIP3 activates AKT signaling
R-MMU-1433557 Signaling by SCF-KIT
R-MMU-1433559 Regulation of KIT signaling
R-MMU-202424 Downstream TCR signaling
R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains
R-MMU-202430 Translocation of ZAP-70 to Immunological synapse
R-MMU-202433 Generation of second messenger molecules
R-MMU-2424491 DAP12 signaling
R-MMU-389356 CD28 co-stimulation
R-MMU-389357 CD28 dependent PI3K/Akt signaling
R-MMU-389359 CD28 dependent Vav1 pathway
R-MMU-389513 CTLA4 inhibitory signaling
R-MMU-389948 PD-1 signaling
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-MMU-9020558 Interleukin-2 signaling

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P06240
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000000409 Expressed in 161 organ(s), highest expression level in thymus
CleanExⁱ	MM_LCK
ExpressionAtlasⁱ	P06240 baseline and differential
Genevisibleⁱ	P06240 MM

Family and domain databases

CDDⁱ	cd10362 SH2_Src_Lck, 1 hit cd12005 SH3_Lck, 1 hit
Gene3Dⁱ	3.30.505.10, 1 hit
InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR035850 Lck_SH2 IPR035749 Lck_SH3 IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR000980 SH2 IPR036860 SH2_dom_sf IPR036028 SH3-like_dom_sf IPR001452 SH3_domain IPR008266 Tyr_kinase_AS IPR020635 Tyr_kinase_cat_dom
Pfamⁱ	View protein in Pfam PF07714 Pkinase_Tyr, 1 hit PF00017 SH2, 1 hit PF00018 SH3_1, 1 hit
PRINTSⁱ	PR00401 SH2DOMAIN PR00452 SH3DOMAIN PR00109 TYRKINASE
SMARTⁱ	View protein in SMART SM00252 SH2, 1 hit SM00326 SH3, 1 hit SM00219 TyrKc, 1 hit
SUPFAMⁱ	SSF50044 SSF50044, 1 hit SSF55550 SSF55550, 1 hit SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00109 PROTEIN_KINASE_TYR, 1 hit PS50001 SH2, 1 hit PS50002 SH3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	LCK_MOUSE
Accessionⁱ	P06240Primary (citable) accession number: P06240 Secondary accession number(s): Q61794 , Q61795, Q62320, Q91X65
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
	Last sequence update:	January 23, 2007
	Last modified:	September 12, 2018
	This is version 213 of the entry and version 4 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human and mouse protein kinases
Human and mouse protein kinases: classification and index
SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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UniProtKB - P06240 (LCK_MOUSE)

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Proto-oncogene tyrosine-protein kinase LCK

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ