rpoN

Functionⁱ

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core RNA polymerase) serves as the receptor for receipt of the melting signal from the remotely bound activator protein NifA for the expression of the nitrogen fixation proteins.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	366 – 385	H-T-H motifBy similarityAdd BLAST		20

GO - Molecular functionⁱ

bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding Source: InterPro
DNA binding Source: UniProtKB-KW
DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
sigma factor activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA-templated transcription, initiation Source: InterPro
nitrogen fixation Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Nucleotidyltransferase, Sigma factor, Transferase
Biological process	Nitrogen fixation, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: RNA polymerase sigma-54 factor
Gene namesⁱ	Name:rpoN Synonyms:ntrA
Organismⁱ	Klebsiella oxytoca
Taxonomic identifierⁱ	571 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Klebsiella

Subcellular locationⁱ

Keywords - Cellular componentⁱ

DNA-directed RNA polymerase

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000205533	1 – 477	RNA polymerase sigma-54 factorAdd BLAST		477

Proteomic databases

PRIDEⁱ	P06223

PRIDEⁱ

P06223

Structureⁱ

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5NSR	electron microscopy	3.80	M	1-477	[»]
	5NSS	electron microscopy	5.80	M	1-477	[»]
ProteinModelPortalⁱ	P06223
SMRⁱ	P06223
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	454 – 462	RPON box		9

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	3 – 39	Gln-richAdd BLAST		37

Sequence similaritiesⁱ

Belongs to the sigma-54 factor family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105DTW Bacteria COG1508 LUCA

eggNOGⁱ

ENOG4105DTW Bacteria
COG1508 LUCA

Family and domain databases

Gene3Dⁱ	1.10.10.1330, 1 hit
InterProⁱ	View protein in InterPro IPR000394 RNA_pol_sigma_54 IPR007046 RNA_pol_sigma_54_core-bd IPR007634 RNA_pol_sigma_54_DNA-bd IPR038709 RpoN_core-bd_sf
PANTHERⁱ	PTHR32248 PTHR32248, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00309 Sigma54_AID, 1 hit PF04963 Sigma54_CBD, 1 hit PF04552 Sigma54_DBD, 1 hit
PIRSFⁱ	PIRSF000774 RpoN, 1 hit
PRINTSⁱ	PR00045 SIGMA54FCT
TIGRFAMsⁱ	TIGR02395 rpoN_sigma, 1 hit
PROSITEⁱ	View protein in PROSITE PS00717 SIGMA54_1, 1 hit PS00718 SIGMA54_2, 1 hit PS50044 SIGMA54_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P06223-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKQGLQLRLS QQLAMTPQLQ QAIRLLQLST LELQQELQQA LDSNPLLEQT 
        60         70         80         90        100
DLHDEVETKE AEDRESLDTV DALEQKEMPE ELPLDASWDE IYTAGTPSGN 
       110        120        130        140        150
GVDYQDDELP VYQGETTQSL QDYLMWQVEL TPFTDTDRAI ATSIVDAVDD 
       160        170        180        190        200
TGYLTISVED IVESIGDDEI GLEEVEAVLK RIQRFDPVGV AAKDLRDCLL 
       210        220        230        240        250
VQLSQFAKET PWIEEARLII SDHLDLLANH DFRSLMRVTR LKEEVLKEAV 
       260        270        280        290        300
NLIQSLDPRP GQSIQTGEPE YVIPDVLVRK VNDRWVVELN SDSLPRLKIN 
       310        320        330        340        350
QQYAAMGNST RNDADGQFIR SNLQEARWLI KSLESRNDTL LRVSRCIVEQ 
       360        370        380        390        400
QQAFFEQGEE FMKPMVLADI AQAVEMHEST ISRVTTQKYL HSPRGIFELK 
       410        420        430        440        450
YFFSSHVNTE GGGEASSTAI RALVKKLIAA ENPAKPLSDS KLTTMLSDQG 
       460        470 
IMVARRTVAK YRESLSIPPS NQRKQLV

Length:477

Mass (Da):53,927

Last modified:January 1, 1988 - v1

Checksum:ⁱ481F29B66B3DCF9A

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X03147 Genomic DNA Translation: CAA26925.1 X16335 Genomic DNA Translation: CAA34390.1
PIRⁱ	A24115
NCBI Reference Sequences More...RefSeqⁱ	WP_049087000.1, NZ_JWBI01000247.1

Protein	Similar proteins		Species	Score	Length
P06223	RNA polymerase sigma-54 factor		Klebsiella grimontii		477
RNA polymerase factor sigma-54 (Fragment)		Bacillus sp. AFS073361		77
RNA polymerase sigma-54 factor		ENTCL		508
UPI0003130B98		9ENTR		477
Uncharacterized protein (Fragment)		CITFR		76
+254

Protein	Similar proteins		Species	Score	Length
P06223	RNA polymerase sigma-54 factor	)	ECOLI		477
RNA polymerase, sigma 54 (Sigma N) factor		uncultured bacterium Contig357		477
RNA polymerase sigma-54 factor		ECOLX		477
RNA polymerase sigma-54 factor		SHIBO		477
RNA polymerase sigma-54 factor		Escherichia coli 2-210-07_S3_C3		477
+2943

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X03147 Genomic DNA Translation: CAA26925.1 X16335 Genomic DNA Translation: CAA34390.1
PIRⁱ	A24115
NCBI Reference Sequences More...RefSeqⁱ	WP_049087000.1, NZ_JWBI01000247.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5NSR	electron microscopy	3.80	M	1-477	[»]
	5NSS	electron microscopy	5.80	M	1-477	[»]
ProteinModelPortalⁱ	P06223
SMRⁱ	P06223
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Proteomic databases

PRIDEⁱ	P06223

PRIDEⁱ

P06223

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Phylogenomic databases

eggNOGⁱ	ENOG4105DTW Bacteria COG1508 LUCA

eggNOGⁱ

ENOG4105DTW Bacteria
COG1508 LUCA

Family and domain databases

Gene3Dⁱ	1.10.10.1330, 1 hit
InterProⁱ	View protein in InterPro IPR000394 RNA_pol_sigma_54 IPR007046 RNA_pol_sigma_54_core-bd IPR007634 RNA_pol_sigma_54_DNA-bd IPR038709 RpoN_core-bd_sf
PANTHERⁱ	PTHR32248 PTHR32248, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00309 Sigma54_AID, 1 hit PF04963 Sigma54_CBD, 1 hit PF04552 Sigma54_DBD, 1 hit
PIRSFⁱ	PIRSF000774 RpoN, 1 hit
PRINTSⁱ	PR00045 SIGMA54FCT
TIGRFAMsⁱ	TIGR02395 rpoN_sigma, 1 hit
PROSITEⁱ	View protein in PROSITE PS00717 SIGMA54_1, 1 hit PS00718 SIGMA54_2, 1 hit PS50044 SIGMA54_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RP54_KLEOX
Accessionⁱ	P06223Primary (citable) accession number: P06223
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
	Last sequence update:	January 1, 1988
	Last modified:	June 20, 2018
	This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

UniProtKB - P06223 (RP54_KLEOX)

Your basket is currently empty. ⁱ

RNA polymerase sigma-54 factor

Select a section on the left to see content.

Functionⁱ

Regions

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Molecule processing

Proteomic databases

Structureⁱ

3D structure databases

Family & Domainsⁱ

Motif

Compositional bias

Sequence similaritiesⁱ

Phylogenomic databases

Family and domain databases

Sequenceⁱ

Sequence databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Phylogenomic databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

UniProt is an ELIXIR core data resource

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P06223 (RP54_KLEOX)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

RNA polymerase sigma-54 factor

rpoN

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ