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Protein

RNA polymerase sigma-54 factor

Gene

rpoN

Organism
Klebsiella oxytoca
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core RNA polymerase) serves as the receptor for receipt of the melting signal from the remotely bound activator protein NifA for the expression of the nitrogen fixation proteins.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi366 – 385H-T-H motifBy similarityAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Sigma factor, Transferase
Biological processNitrogen fixation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma-54 factor
Gene namesi
Name:rpoN
Synonyms:ntrA
OrganismiKlebsiella oxytoca
Taxonomic identifieri571 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002055331 – 477RNA polymerase sigma-54 factorAdd BLAST477

Proteomic databases

PRIDEiP06223

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5NSRelectron microscopy3.80M1-477[»]
5NSSelectron microscopy5.80M1-477[»]
ProteinModelPortaliP06223
SMRiP06223
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi454 – 462RPON box9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 39Gln-richAdd BLAST37

Sequence similaritiesi

Belongs to the sigma-54 factor family.Curated

Phylogenomic databases

eggNOGiENOG4105DTW Bacteria
COG1508 LUCA

Family and domain databases

Gene3Di1.10.10.1330, 1 hit
InterProiView protein in InterPro
IPR000394 RNA_pol_sigma_54
IPR007046 RNA_pol_sigma_54_core-bd
IPR007634 RNA_pol_sigma_54_DNA-bd
IPR038709 RpoN_core-bd_sf
PANTHERiPTHR32248 PTHR32248, 1 hit
PfamiView protein in Pfam
PF00309 Sigma54_AID, 1 hit
PF04963 Sigma54_CBD, 1 hit
PF04552 Sigma54_DBD, 1 hit
PIRSFiPIRSF000774 RpoN, 1 hit
PRINTSiPR00045 SIGMA54FCT
TIGRFAMsiTIGR02395 rpoN_sigma, 1 hit
PROSITEiView protein in PROSITE
PS00717 SIGMA54_1, 1 hit
PS00718 SIGMA54_2, 1 hit
PS50044 SIGMA54_3, 1 hit

Sequencei

Sequence statusi: Complete.

P06223-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQGLQLRLS QQLAMTPQLQ QAIRLLQLST LELQQELQQA LDSNPLLEQT
60 70 80 90 100
DLHDEVETKE AEDRESLDTV DALEQKEMPE ELPLDASWDE IYTAGTPSGN
110 120 130 140 150
GVDYQDDELP VYQGETTQSL QDYLMWQVEL TPFTDTDRAI ATSIVDAVDD
160 170 180 190 200
TGYLTISVED IVESIGDDEI GLEEVEAVLK RIQRFDPVGV AAKDLRDCLL
210 220 230 240 250
VQLSQFAKET PWIEEARLII SDHLDLLANH DFRSLMRVTR LKEEVLKEAV
260 270 280 290 300
NLIQSLDPRP GQSIQTGEPE YVIPDVLVRK VNDRWVVELN SDSLPRLKIN
310 320 330 340 350
QQYAAMGNST RNDADGQFIR SNLQEARWLI KSLESRNDTL LRVSRCIVEQ
360 370 380 390 400
QQAFFEQGEE FMKPMVLADI AQAVEMHEST ISRVTTQKYL HSPRGIFELK
410 420 430 440 450
YFFSSHVNTE GGGEASSTAI RALVKKLIAA ENPAKPLSDS KLTTMLSDQG
460 470
IMVARRTVAK YRESLSIPPS NQRKQLV
Length:477
Mass (Da):53,927
Last modified:January 1, 1988 - v1
Checksum:i481F29B66B3DCF9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03147 Genomic DNA Translation: CAA26925.1
X16335 Genomic DNA Translation: CAA34390.1
PIRiA24115
RefSeqiWP_049087000.1, NZ_JWBI01000247.1

Similar proteinsi

Entry informationi

Entry nameiRP54_KLEOX
AccessioniPrimary (citable) accession number: P06223
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 20, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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