UniProtKB - P06223 (RP54_KLEOX)
Protein
RNA polymerase sigma-54 factor
Gene
rpoN
Organism
Klebsiella oxytoca
Status
Functioni
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core RNA polymerase) serves as the receptor for receipt of the melting signal from the remotely bound activator protein NifA for the expression of the nitrogen fixation proteins.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 366 – 385 | H-T-H motifBy similarityAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription activator activity Source: InterPro
- DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- sigma factor activity Source: UniProtKB-KW
GO - Biological processi
- DNA-templated transcription, initiation Source: InterPro
- nitrogen fixation Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Nucleotidyltransferase, Sigma factor, Transferase |
Biological process | Nitrogen fixation, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: RNA polymerase sigma-54 factor |
Gene namesi | Name:rpoN Synonyms:ntrA |
Organismi | Klebsiella oxytoca |
Taxonomic identifieri | 571 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Klebsiella |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205533 | 1 – 477 | RNA polymerase sigma-54 factorAdd BLAST | 477 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P06223 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 454 – 462 | RPON box | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 3 – 39 | Gln-richAdd BLAST | 37 |
Sequence similaritiesi
Belongs to the sigma-54 factor family.Curated
Phylogenomic databases
eggNOGi | COG1508, Bacteria |
OrthoDBi | 1906842at2 |
Family and domain databases
Gene3Di | 1.10.10.1330, 1 hit |
InterProi | View protein in InterPro IPR000394, RNA_pol_sigma_54 IPR007046, RNA_pol_sigma_54_core-bd IPR007634, RNA_pol_sigma_54_DNA-bd IPR038709, RpoN_core-bd_sf |
PANTHERi | PTHR32248, PTHR32248, 1 hit |
Pfami | View protein in Pfam PF00309, Sigma54_AID, 1 hit PF04963, Sigma54_CBD, 1 hit PF04552, Sigma54_DBD, 1 hit |
PIRSFi | PIRSF000774, RpoN, 1 hit |
PRINTSi | PR00045, SIGMA54FCT |
TIGRFAMsi | TIGR02395, rpoN_sigma, 1 hit |
PROSITEi | View protein in PROSITE PS00717, SIGMA54_1, 1 hit PS00718, SIGMA54_2, 1 hit PS50044, SIGMA54_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P06223-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKQGLQLRLS QQLAMTPQLQ QAIRLLQLST LELQQELQQA LDSNPLLEQT
60 70 80 90 100
DLHDEVETKE AEDRESLDTV DALEQKEMPE ELPLDASWDE IYTAGTPSGN
110 120 130 140 150
GVDYQDDELP VYQGETTQSL QDYLMWQVEL TPFTDTDRAI ATSIVDAVDD
160 170 180 190 200
TGYLTISVED IVESIGDDEI GLEEVEAVLK RIQRFDPVGV AAKDLRDCLL
210 220 230 240 250
VQLSQFAKET PWIEEARLII SDHLDLLANH DFRSLMRVTR LKEEVLKEAV
260 270 280 290 300
NLIQSLDPRP GQSIQTGEPE YVIPDVLVRK VNDRWVVELN SDSLPRLKIN
310 320 330 340 350
QQYAAMGNST RNDADGQFIR SNLQEARWLI KSLESRNDTL LRVSRCIVEQ
360 370 380 390 400
QQAFFEQGEE FMKPMVLADI AQAVEMHEST ISRVTTQKYL HSPRGIFELK
410 420 430 440 450
YFFSSHVNTE GGGEASSTAI RALVKKLIAA ENPAKPLSDS KLTTMLSDQG
460 470
IMVARRTVAK YRESLSIPPS NQRKQLV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03147 Genomic DNA Translation: CAA26925.1 X16335 Genomic DNA Translation: CAA34390.1 |
PIRi | A24115 |
RefSeqi | WP_049087000.1, NZ_QPKC01000013.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03147 Genomic DNA Translation: CAA26925.1 X16335 Genomic DNA Translation: CAA34390.1 |
PIRi | A24115 |
RefSeqi | WP_049087000.1, NZ_QPKC01000013.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5NSR | electron microscopy | 3.80 | M | 1-477 | [»] | |
5NSS | electron microscopy | 5.80 | M | 1-477 | [»] | |
6GFW | electron microscopy | 3.70 | M | 1-477 | [»] | |
6GH5 | electron microscopy | 3.40 | M | 1-477 | [»] | |
6GH6 | electron microscopy | 4.10 | M | 1-477 | [»] | |
SMRi | P06223 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 571.MC52_05295 |
Phylogenomic databases
eggNOGi | COG1508, Bacteria |
OrthoDBi | 1906842at2 |
Family and domain databases
Gene3Di | 1.10.10.1330, 1 hit |
InterProi | View protein in InterPro IPR000394, RNA_pol_sigma_54 IPR007046, RNA_pol_sigma_54_core-bd IPR007634, RNA_pol_sigma_54_DNA-bd IPR038709, RpoN_core-bd_sf |
PANTHERi | PTHR32248, PTHR32248, 1 hit |
Pfami | View protein in Pfam PF00309, Sigma54_AID, 1 hit PF04963, Sigma54_CBD, 1 hit PF04552, Sigma54_DBD, 1 hit |
PIRSFi | PIRSF000774, RpoN, 1 hit |
PRINTSi | PR00045, SIGMA54FCT |
TIGRFAMsi | TIGR02395, rpoN_sigma, 1 hit |
PROSITEi | View protein in PROSITE PS00717, SIGMA54_1, 1 hit PS00718, SIGMA54_2, 1 hit PS50044, SIGMA54_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RP54_KLEOX | |
Accessioni | P06223Primary (citable) accession number: P06223 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | February 10, 2021 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families