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Protein

2-isopropylmalate synthase

Gene

LEU4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).

Miscellaneous

Present with 6630 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.

Pathwayi: L-leucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase 2, mitochondrial (LEU9), 2-isopropylmalate synthase (LEU4)
  2. 3-isopropylmalate dehydratase (LEU1)
  3. 3-isopropylmalate dehydrogenase (LEU2)
  4. Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • 2-isopropylmalate synthase activity Source: SGD

GO - Biological processi

  • leucine biosynthetic process Source: SGD

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YNL104C-MONOMER
UniPathwayi
UPA00048;UER00070

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthase (EC:2.3.3.13)
Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase
Gene namesi
Name:LEU4
Ordered Locus Names:YNL104C
ORF Names:N2173
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

SGDiS000005048 LEU4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000010461 – 6192-isopropylmalate synthaseAdd BLAST619

Proteomic databases

PaxDbiP06208
PeptideAtlasiP06208
PRIDEiP06208

PTM databases

iPTMnetiP06208

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
LEU9Q121663EBI-10116,EBI-37359

Protein-protein interaction databases

BioGridi35719, 125 interactors
DIPiDIP-1412N
IntActiP06208, 5 interactors
MINTiP06208
STRINGi4932.YNL104C

Structurei

3D structure databases

ProteinModelPortaliP06208
SMRiP06208
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 336Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST276

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000018527
HOGENOMiHOG000110941
InParanoidiP06208
KOiK01649
OMAiYSPETFC
OrthoDBiEOG092C18QN

Family and domain databases

CDDicd07942 DRE_TIM_LeuA, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00572 LeuA_type2, 1 hit
InterProiView protein in InterPro
IPR013709 2-isopropylmalate_synth_dimer
IPR002034 AIPM/Hcit_synth_CS
IPR013785 Aldolase_TIM
IPR005668 IPM_Synthase
IPR036230 LeuA_allosteric_dom_sf
IPR039371 LeuA_N_DRE-TIM
IPR000891 PYR_CT
PfamiView protein in Pfam
PF00682 HMGL-like, 1 hit
PF08502 LeuA_dimer, 1 hit
SMARTiView protein in SMART
SM00917 LeuA_dimer, 1 hit
SUPFAMiSSF110921 SSF110921, 1 hit
TIGRFAMsiTIGR00970 leuA_yeast, 1 hit
PROSITEiView protein in PROSITE
PS00815 AIPM_HOMOCIT_SYNTH_1, 1 hit
PS00816 AIPM_HOMOCIT_SYNTH_2, 1 hit
PS50991 PYR_CT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket
Isoform Mitochondrial (identifier: P06208-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVKESIIALA EHAASRASRV IPPVKLAYKN MLKDPSSKYK PFNAPKLSNR
60 70 80 90 100
KWPDNRITRA PRWLSTDLRD GNQSLPDPMS VEQKKEYFHK LVNIGFKEIE
110 120 130 140 150
VSFPSASQTD FDFTRYAVEN APDDVSIQCL VQSREHLIKR TVEALTGAKK
160 170 180 190 200
ATIHTYLATS DMFREIVFNM SREEAISKAV EATKLVRKLT KDDPSQQATR
210 220 230 240 250
WSYEFSPECF SDTPGEFAVE ICEAVKKAWE PTEENPIIFN LPATVEVASP
260 270 280 290 300
NVYADQIEYF ATHITEREKV CISTHCHNDR GCGVAATELG MLAGADRVEG
310 320 330 340 350
CLFGNGERTG NVDLVTVAMN MYTQGVSPNL DFSDLTSVLD VVERCNKIPV
360 370 380 390 400
SQRAPYGGDL VVCAFSGSHQ DAIKKGFNLQ NKKRAQGETQ WRIPYLPLDP
410 420 430 440 450
KDIGRDYEAV IRVNSQSGKG GAAWVILRSL GLDLPRNMQI EFSSAVQDHA
460 470 480 490 500
DSLGRELKSD EISKLFKEAY NYNDEQYQAI SLVNYNVEKF GTERRVFTGQ
510 520 530 540 550
VKVGDQIVDI EGTGNGPISS LVDALSNLLN VRFAVANYTE HSLGSGSSTQ
560 570 580 590 600
AASYIHLSYR RNADNEKAYK WGVGVSEDVG DSSVRAIFAT INNIIHSGDV
610
SIPSLAEVEG KNAAASGSA
Length:619
Mass (Da):68,409
Last modified:January 1, 1988 - v1
Checksum:i6886E76C5DD89CE2
GO
Isoform Cytoplasmic (identifier: P06208-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Note: Produced by alternative initiation at Met-31 of isoform Mitochondrial.
Show »
Length:589
Mass (Da):65,163
Checksum:iA920218A02F29D99
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186401 – 30Missing in isoform Cytoplasmic. CuratedAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12893 Genomic DNA Translation: AAA34743.1
M12893 Genomic DNA Translation: AAA34744.1
Z50161 Genomic DNA Translation: CAA90522.1
Z71380 Genomic DNA Translation: CAA95980.1
Z12126 Genomic DNA Translation: CAA78110.1
BK006947 Genomic DNA Translation: DAA10441.1
PIRiA23872
RefSeqiNP_014295.1, NM_001182942.1 [P06208-1]

Genome annotation databases

EnsemblFungiiYNL104C; YNL104C; YNL104C [P06208-1]
GeneIDi855619
KEGGisce:YNL104C

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12893 Genomic DNA Translation: AAA34743.1
M12893 Genomic DNA Translation: AAA34744.1
Z50161 Genomic DNA Translation: CAA90522.1
Z71380 Genomic DNA Translation: CAA95980.1
Z12126 Genomic DNA Translation: CAA78110.1
BK006947 Genomic DNA Translation: DAA10441.1
PIRiA23872
RefSeqiNP_014295.1, NM_001182942.1 [P06208-1]

3D structure databases

ProteinModelPortaliP06208
SMRiP06208
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35719, 125 interactors
DIPiDIP-1412N
IntActiP06208, 5 interactors
MINTiP06208
STRINGi4932.YNL104C

PTM databases

iPTMnetiP06208

Proteomic databases

PaxDbiP06208
PeptideAtlasiP06208
PRIDEiP06208

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL104C; YNL104C; YNL104C [P06208-1]
GeneIDi855619
KEGGisce:YNL104C

Organism-specific databases

SGDiS000005048 LEU4

Phylogenomic databases

GeneTreeiENSGT00390000018527
HOGENOMiHOG000110941
InParanoidiP06208
KOiK01649
OMAiYSPETFC
OrthoDBiEOG092C18QN

Enzyme and pathway databases

UniPathwayi
UPA00048;UER00070

BioCyciYEAST:YNL104C-MONOMER

Miscellaneous databases

PROiPR:P06208

Family and domain databases

CDDicd07942 DRE_TIM_LeuA, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00572 LeuA_type2, 1 hit
InterProiView protein in InterPro
IPR013709 2-isopropylmalate_synth_dimer
IPR002034 AIPM/Hcit_synth_CS
IPR013785 Aldolase_TIM
IPR005668 IPM_Synthase
IPR036230 LeuA_allosteric_dom_sf
IPR039371 LeuA_N_DRE-TIM
IPR000891 PYR_CT
PfamiView protein in Pfam
PF00682 HMGL-like, 1 hit
PF08502 LeuA_dimer, 1 hit
SMARTiView protein in SMART
SM00917 LeuA_dimer, 1 hit
SUPFAMiSSF110921 SSF110921, 1 hit
TIGRFAMsiTIGR00970 leuA_yeast, 1 hit
PROSITEiView protein in PROSITE
PS00815 AIPM_HOMOCIT_SYNTH_1, 1 hit
PS00816 AIPM_HOMOCIT_SYNTH_2, 1 hit
PS50991 PYR_CT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLEU1_YEAST
AccessioniPrimary (citable) accession number: P06208
Secondary accession number(s): D6W175
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 7, 2018
This is version 178 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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