UniProtKB - P06199 (ACM2_PIG)
Protein
Muscarinic acetylcholine receptor M2
Gene
CHRM2
Organism
Sus scrofa (Pig)
Status
Functioni
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.1 Publication
GO - Molecular functioni
- G protein-coupled acetylcholine receptor activity Source: UniProtKB
GO - Biological processi
- G protein-coupled acetylcholine receptor signaling pathway Source: UniProtKB
- regulation of heart contraction Source: InterPro
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Names & Taxonomyi
Protein namesi | Recommended name: Muscarinic acetylcholine receptor M2 |
Gene namesi | Name:CHRM2 |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane 3 Publications; Multi-pass membrane protein Curated
- postsynaptic cell membrane Curated; Multi-pass membrane protein Curated
Note: Phosphorylation in response to agonist binding promotes receptor internalization.1 Publication
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- postsynaptic membrane Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 22 | ExtracellularBy similarityAdd BLAST | 22 | |
Transmembranei | 23 – 45 | Helical; Name=1By similarityAdd BLAST | 23 | |
Topological domaini | 46 – 59 | CytoplasmicBy similarityAdd BLAST | 14 | |
Transmembranei | 60 – 80 | Helical; Name=2By similarityAdd BLAST | 21 | |
Topological domaini | 81 – 97 | ExtracellularBy similarityAdd BLAST | 17 | |
Transmembranei | 98 – 119 | Helical; Name=3By similarityAdd BLAST | 22 | |
Topological domaini | 120 – 139 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 140 – 162 | Helical; Name=4By similarityAdd BLAST | 23 | |
Topological domaini | 163 – 184 | ExtracellularBy similarityAdd BLAST | 22 | |
Transmembranei | 185 – 209 | Helical; Name=5By similarityAdd BLAST | 25 | |
Topological domaini | 210 – 387 | CytoplasmicBy similarityAdd BLAST | 178 | |
Transmembranei | 388 – 410 | Helical; Name=6By similarityAdd BLAST | 23 | |
Topological domaini | 411 – 418 | ExtracellularBy similarity | 8 | |
Transmembranei | 419 – 442 | Helical; Name=7By similarityAdd BLAST | 24 | |
Topological domaini | 443 – 466 | CytoplasmicBy similarityAdd BLAST | 24 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069024 | 1 – 466 | Muscarinic acetylcholine receptor M2Add BLAST | 466 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 2 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 3 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 6 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 96 ↔ 176 | PROSITE-ProRule annotation | ||
Modified residuei | 232 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 413 ↔ 416 | PROSITE-ProRule annotation | ||
Modified residuei | 446 | PhosphothreonineSequence analysis | 1 | |
Modified residuei | 450 | PhosphothreonineSequence analysis | 1 | |
Modified residuei | 465 | PhosphothreonineSequence analysis | 1 |
Post-translational modificationi
Phosphorylated in response to agonist treatment.1 Publication
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PRIDEi | P06199 |
Interactioni
Subunit structurei
Interacts with ARRB1 and ARRB2 (By similarity).
Interacts with RACK1; the interaction regulates CHRM2 internalization.
By similarity1 PublicationProtein-protein interaction databases
IntActi | P06199, 1 interactor |
Chemistry databases
BindingDBi | P06199 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 103 – 107 | Agonist bindingBy similarity | 5 | |
Regioni | 403 – 404 | Agonist bindingBy similarity | 2 | |
Regioni | 426 – 430 | Agonist bindingBy similarity | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 120 – 122 | Important for signaling | 3 | |
Motifi | 436 – 440 | Important for signaling | 5 |
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
InParanoidi | P06199 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001065, Musac_Ach_M2_rcpt IPR000995, Musac_Ach_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00243, MUSCARINICR PR00539, MUSCRINICM2R |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P06199-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNNSTNSSNS GLALTSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV
60 70 80 90 100
NRHLQTVNNY FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL
110 120 130 140 150
ALDYVVSNAS VMNLLIISFD RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL
160 170 180 190 200
SFILWAPAIL FWQFIVGVRT VEDGECYIQF FSNAAVTFGT AIAAFYLPVI
210 220 230 240 250
IMTVLYWHIS RASKSRIKKD KKEPVANQEP VSPSLVQGRI VKPNNNNMPG
260 270 280 290 300
SDEALEHNKI QNGKAPRDAV TENCVQGEEK ESSNDSTSVS AVASNMRDDE
310 320 330 340 350
ITQDENTVST SLGHSKDENS KQTCIKIVTK TQKSDSCTPA NTTVELVGSS
360 370 380 390 400
GQNGDEKQNI VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW
410 420 430 440 450
APYNVMVLIN TFCAPCIPNT VWTIGYWLCY INSTINPACY ALCNATFKKT
460
FKHLLMCHYK NIGATR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 330 | K → N in AAA30986 (PubMed:3107123).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04708 mRNA Translation: CAA28413.1 M16331 Genomic DNA Translation: AAA30986.1 |
PIRi | A27386 |
RefSeqi | NP_999426.1, NM_214261.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04708 mRNA Translation: CAA28413.1 M16331 Genomic DNA Translation: AAA30986.1 |
PIRi | A27386 |
RefSeqi | NP_999426.1, NM_214261.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | P06199, 1 interactor |
Chemistry databases
BindingDBi | P06199 |
ChEMBLi | CHEMBL4781 |
DrugCentrali | P06199 |
Protein family/group databases
GPCRDBi | Search... |
Proteomic databases
PRIDEi | P06199 |
Genome annotation databases
Organism-specific databases
CTDi | 1129 |
Phylogenomic databases
InParanoidi | P06199 |
Miscellaneous databases
PROi | PR:P06199 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001065, Musac_Ach_M2_rcpt IPR000995, Musac_Ach_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00243, MUSCARINICR PR00539, MUSCRINICM2R |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACM2_PIG | |
Accessioni | P06199Primary (citable) accession number: P06199 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | February 10, 2021 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - SIMILARITY comments
Index of protein domains and families