CHRM2

Functionⁱ

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.1 Publication

GO - Molecular functionⁱ

G protein-coupled acetylcholine receptor activity Source: UniProtKB
G protein-coupled receptor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
G protein-coupled serotonin receptor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
neurotransmitter receptor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
chemical synaptic transmission
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
G protein-coupled acetylcholine receptor signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of heart contraction Source: InterPro
regulation of smooth muscle contraction
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	G-protein coupled receptor, Receptor, Transducer

Molecular function

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Muscarinic acetylcholine receptor M2
Gene namesⁱ	Name:CHRM2
Organismⁱ	Sus scrofa (Pig)
Taxonomic identifierⁱ	9823 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus
Proteomesⁱ	UP000008227 Componentⁱ: Unplaced

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 22	ExtracellularBy similarityAdd BLAST	22
Transmembraneⁱ	23 – 45	Helical; Name=1By similarityAdd BLAST	23
Topological domainⁱ	46 – 59	CytoplasmicBy similarityAdd BLAST	14
Transmembraneⁱ	60 – 80	Helical; Name=2By similarityAdd BLAST	21
Topological domainⁱ	81 – 97	ExtracellularBy similarityAdd BLAST	17
Transmembraneⁱ	98 – 119	Helical; Name=3By similarityAdd BLAST	22
Topological domainⁱ	120 – 139	CytoplasmicBy similarityAdd BLAST	20
Transmembraneⁱ	140 – 162	Helical; Name=4By similarityAdd BLAST	23
Topological domainⁱ	163 – 184	ExtracellularBy similarityAdd BLAST	22
Transmembraneⁱ	185 – 209	Helical; Name=5By similarityAdd BLAST	25
Topological domainⁱ	210 – 387	CytoplasmicBy similarityAdd BLAST	178
Transmembraneⁱ	388 – 410	Helical; Name=6By similarityAdd BLAST	23
Topological domainⁱ	411 – 418	ExtracellularBy similarity	8
Transmembraneⁱ	419 – 442	Helical; Name=7By similarityAdd BLAST	24
Topological domainⁱ	443 – 466	CytoplasmicBy similarityAdd BLAST	24

Keywords - Cellular componentⁱ

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL4781

ChEMBLⁱ

CHEMBL4781

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000069024	1 – 466	Muscarinic acetylcholine receptor M2Add BLAST		466

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	2	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	3	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	6	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	96 ↔ 176	PROSITE-ProRule annotation
Modified residueⁱ	232	PhosphoserineBy similarity	1
Disulfide bondⁱ	413 ↔ 416	PROSITE-ProRule annotation
Modified residueⁱ	446	PhosphothreonineSequence analysis	1
Modified residueⁱ	450	PhosphothreonineSequence analysis	1
Modified residueⁱ	465	PhosphothreonineSequence analysis	1

Post-translational modificationⁱ

Phosphorylated in response to agonist treatment.1 Publication

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEⁱ	P06199

PRIDEⁱ

P06199

Interactionⁱ

Subunit structureⁱ

Interacts with ARRB1 and ARRB2 (By similarity). Interacts with RACK1; the interaction regulates CHRM2 internalization.By similarity1 Publication

Protein-protein interaction databases

IntActⁱ	P06199, 1 interactor

IntActⁱ

P06199, 1 interactor

Chemistry databases

BindingDBⁱ

P06199

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P06199
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	103 – 107	Agonist bindingBy similarity	5
Regionⁱ	403 – 404	Agonist bindingBy similarity	2
Regionⁱ	426 – 430	Agonist bindingBy similarity	5

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	120 – 122	Important for signaling		3
Motifⁱ	436 – 440	Important for signaling		5

Sequence similaritiesⁱ

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENⁱ	HBG105720
InParanoidⁱ	P06199
KEGG Orthology (KO) More...KOⁱ	K04130

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd15297 7tmA_mAChR_M2, 1 hit
InterProⁱ	View protein in InterPro IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM IPR001065 Musac_Ach_M2_rcpt IPR000995 Musac_Ach_rcpt
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00001 7tm_1, 1 hit
PRINTSⁱ	PR00237 GPCRRHODOPSN PR00243 MUSCARINICR PR00539 MUSCRINICM2R
PROSITEⁱ	View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P06199-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNNSTNSSNS GLALTSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV 
        60         70         80         90        100
NRHLQTVNNY FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL 
       110        120        130        140        150
ALDYVVSNAS VMNLLIISFD RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL 
       160        170        180        190        200
SFILWAPAIL FWQFIVGVRT VEDGECYIQF FSNAAVTFGT AIAAFYLPVI 
       210        220        230        240        250
IMTVLYWHIS RASKSRIKKD KKEPVANQEP VSPSLVQGRI VKPNNNNMPG 
       260        270        280        290        300
SDEALEHNKI QNGKAPRDAV TENCVQGEEK ESSNDSTSVS AVASNMRDDE 
       310        320        330        340        350
ITQDENTVST SLGHSKDENS KQTCIKIVTK TQKSDSCTPA NTTVELVGSS 
       360        370        380        390        400
GQNGDEKQNI VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW 
       410        420        430        440        450
APYNVMVLIN TFCAPCIPNT VWTIGYWLCY INSTINPACY ALCNATFKKT 
       460 
FKHLLMCHYK NIGATR

Length:466

Mass (Da):51,673

Last modified:January 1, 1988 - v1

Checksum:ⁱ53D089F179789CD9

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	330	K → N in AAA30986 (PubMed:3107123).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X04708 mRNA Translation: CAA28413.1 M16331 Genomic DNA Translation: AAA30986.1
PIRⁱ	A27386
NCBI Reference Sequences More...RefSeqⁱ	NP_999426.1, NM_214261.1
UniGeneⁱ	Ssc.16247

Genome annotation databases

GeneIDⁱ	397498
KEGGⁱ	ssc:397498

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P06199	Muscarinic acetylcholine receptor M2	)	HUMAN		466	UniRef90_P08172
Muscarinic acetylcholine receptor M2 (Fragment)		PANTR		440
Muscarinic acetylcholine receptor M2		MOUSE		466
Muscarinic acetylcholine receptor M2		RAT		466
Muscarinic acetylcholine receptor M2		BOVIN		465
+110

Protein	Similar proteins		Species	Score	Length	Source
P06199	Muscarinic acetylcholine receptor M2	)	HUMAN		466	UniRef50_P08172
Muscarinic acetylcholine receptor M2 (Fragment)		PANTR		440
Muscarinic acetylcholine receptor M2		MOUSE		466
Muscarinic acetylcholine receptor M2		RAT		466
Muscarinic acetylcholine receptor M2		BOVIN		465
+429

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X04708 mRNA Translation: CAA28413.1 M16331 Genomic DNA Translation: AAA30986.1
PIRⁱ	A27386
RefSeqⁱ	NP_999426.1, NM_214261.1
UniGeneⁱ	Ssc.16247

3D structure databases

ProteinModelPortalⁱ	P06199
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P06199, 1 interactor

IntActⁱ

P06199, 1 interactor

Chemistry databases

BindingDBⁱ	P06199
ChEMBLⁱ	CHEMBL4781

Protein family/group databases

GPCRDBⁱ	Search...

GPCRDBⁱ

Search...

Proteomic databases

PRIDEⁱ	P06199

PRIDEⁱ

P06199

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	397498
KEGGⁱ	ssc:397498

Organism-specific databases

CTDⁱ	1129

CTDⁱ

1129

Phylogenomic databases

HOVERGENⁱ	HBG105720
InParanoidⁱ	P06199
KOⁱ	K04130

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P06199

PROⁱ

PR:P06199

Family and domain databases

CDDⁱ	cd15297 7tmA_mAChR_M2, 1 hit
InterProⁱ	View protein in InterPro IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM IPR001065 Musac_Ach_M2_rcpt IPR000995 Musac_Ach_rcpt
Pfamⁱ	View protein in Pfam PF00001 7tm_1, 1 hit
PRINTSⁱ	PR00237 GPCRRHODOPSN PR00243 MUSCARINICR PR00539 MUSCRINICM2R
PROSITEⁱ	View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ACM2_PIG
Accessionⁱ	P06199Primary (citable) accession number: P06199
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
	Last sequence update:	January 1, 1988
	Last modified:	November 7, 2018
	This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P06199 (ACM2_PIG)

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Muscarinic acetylcholine receptor M2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ