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Protein

Uroporphyrinogen-III synthase

Gene

HEM4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.By similarity

Miscellaneous

Present with 1360 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be involved in mitochondrial import.1 Publication

Catalytic activityi

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (HEM2)
  2. Porphobilinogen deaminase (HEM3)
  3. Uroporphyrinogen-III synthase (HEM4)
  4. Uroporphyrinogen decarboxylase (HEM12)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • uroporphyrinogen-III synthase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processHeme biosynthesis, Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YOR278W-MONOMER
ReactomeiR-SCE-189451 Heme biosynthesis
UniPathwayi
UPA00251;UER00320

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III synthase (EC:4.2.1.75)
Short name:
UROIIIS
Short name:
UROS
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene namesi
Name:HEM4
Synonyms:ORF1
Ordered Locus Names:YOR278W
ORF Names:O5463
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR278W
SGDiS000005804 HEM4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001352541 – 275Uroporphyrinogen-III synthaseAdd BLAST275

Proteomic databases

MaxQBiP06174
PaxDbiP06174
PRIDEiP06174

Interactioni

Protein-protein interaction databases

BioGridi34666, 112 interactors
IntActiP06174, 2 interactors
STRINGi4932.YOR278W

Structurei

3D structure databases

ProteinModelPortaliP06174
SMRiP06174
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000009853
HOGENOMiHOG000216635
InParanoidiP06174
KOiK01719
OMAiDQIKFAA
OrthoDBiEOG092C36S3

Family and domain databases

CDDicd06578 HemD, 1 hit
InterProiView protein in InterPro
IPR036108 4pyrrol_syn_uPrphyn_synt_sf
IPR003754 4pyrrol_synth_uPrphyn_synth
PfamiView protein in Pfam
PF02602 HEM4, 1 hit
SUPFAMiSSF69618 SSF69618, 1 hit

Sequencei

Sequence statusi: Complete.

P06174-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSRKKVRVL LLKNKTVPID KYELECRSKA FEPIFVPLIK HTHVIQDFRN
60 70 80 90 100
VLNTIPNYLN TINYIIITSQ RTVESLNEAI IPTLTSEQKA ALLSKTVYTV
110 120 130 140 150
GPATANFIRR SGFINVKGGE DAGNGSILAD IIIDDLSTDI KACPPSELLF
160 170 180 190 200
LVGEIRRDII PKKLHSKGIK VREVVTYKTE ELSDGFKRFI HAMKECDEDE
210 220 230 240 250
VFSDWVVVFS PQGTKEITQY LGDSNRLPGS HLRVASIGPT TKKYLDDNDV
260 270
TSDVVSPKPD PKSLLDAIEL YQRHK
Length:275
Mass (Da):30,912
Last modified:October 1, 1996 - v2
Checksum:iCDC857C8CFB4E1A7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti231 – 275HLRVA…YQRHK → IYGLRHRTYH in CAA28399 (PubMed:2834088).CuratedAdd BLAST45

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04694 Genomic DNA Translation: CAA28399.1
X89633 Genomic DNA Translation: CAA61783.1
Z75186 Genomic DNA Translation: CAA99504.1
BK006948 Genomic DNA Translation: DAA11043.1
PIRiS67180 RGBY7K
RefSeqiNP_014921.1, NM_001183697.1

Genome annotation databases

EnsemblFungiiYOR278W; YOR278W; YOR278W
GeneIDi854452
KEGGisce:YOR278W

Similar proteinsi

Entry informationi

Entry nameiHEM4_YEAST
AccessioniPrimary (citable) accession number: P06174
Secondary accession number(s): D6W2X7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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