UniProtKB - P06168 (ILV5_YEAST)
Protein
Ketol-acid reductoisomerase, mitochondrial
Gene
ILV5
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 883000 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:1.1.1.86
- (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + H+ + NADPHEC:1.1.1.86
Cofactori
Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity
: L-isoleucine biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Acetolactate synthase small subunit, mitochondrial (ILV6), Acetolactate synthase catalytic subunit, mitochondrial (ILV2)
- Ketol-acid reductoisomerase, mitochondrial (ILV5)
- Dihydroxy-acid dehydratase, mitochondrial (ILV3)
- Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.
Pathwayi: L-valine biosynthesis
This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Acetolactate synthase small subunit, mitochondrial (ILV6), Acetolactate synthase catalytic subunit, mitochondrial (ILV2)
- Ketol-acid reductoisomerase, mitochondrial (ILV5)
- Dihydroxy-acid dehydratase, mitochondrial (ILV3)
- Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 171 | Sequence analysis | 1 | |
Metal bindingi | 255 | Magnesium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 255 | Magnesium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 259 | Magnesium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 291 | Magnesium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 295 | Magnesium 2PROSITE-ProRule annotation | 1 | |
Binding sitei | 317 | SubstratePROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 84 – 93 | NADPSequence analysis | 10 | |
Nucleotide bindingi | 108 – 113 | NADPBy similarity | 6 | |
Nucleotide bindingi | 146 – 150 | NADPBy similarity | 5 |
GO - Molecular functioni
- double-stranded DNA binding Source: SGD
- ketol-acid reductoisomerase activity Source: SGD
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- branched-chain amino acid biosynthetic process Source: SGD
- isoleucine biosynthetic process Source: GO_Central
- mitochondrial genome maintenance Source: SGD
- valine biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis |
Ligand | Magnesium, Metal-binding, NADP |
Enzyme and pathway databases
UniPathwayi | UPA00047;UER00056 UPA00049;UER00060 |
Protein family/group databases
MoonProti | P06168 |
Names & Taxonomyi
Protein namesi | Recommended name: Ketol-acid reductoisomerase, mitochondrial (EC:1.1.1.86)Alternative name(s): Acetohydroxy-acid reductoisomerase Alpha-keto-beta-hydroxylacyl reductoisomerase |
Gene namesi | Name:ILV5 Ordered Locus Names:YLR355C ORF Names:L9638.7 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004347, ILV5 |
VEuPathDBi | FungiDB:YLR355C |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrial nucleoid Source: SGD
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 47 | MitochondrionSequence analysisAdd BLAST | 47 | |
ChainiPRO_0000015634 | 48 – 395 | Ketol-acid reductoisomerase, mitochondrialAdd BLAST | 348 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 355 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P06168 |
PaxDbi | P06168 |
PRIDEi | P06168 |
TopDownProteomicsi | P06168 |
2D gel databases
SWISS-2DPAGEi | P06168 |
PTM databases
CarbonylDBi | P06168 |
iPTMneti | P06168 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 31617, 133 interactors |
DIPi | DIP-6463N |
IntActi | P06168, 38 interactors |
MINTi | P06168 |
STRINGi | 4932.YLR355C |
Miscellaneous databases
RNActi | P06168, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 57 – 246 | KARI N-terminal RossmannPROSITE-ProRule annotationAdd BLAST | 190 | |
Domaini | 247 – 394 | KARI C-terminal knottedPROSITE-ProRule annotationAdd BLAST | 148 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 363 – 395 | HydrophilicAdd BLAST | 33 |
Sequence similaritiesi
Belongs to the ketol-acid reductoisomerase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | ENOG502QQBF, Eukaryota |
HOGENOMi | CLU_033821_1_2_1 |
InParanoidi | P06168 |
OMAi | RAMFSWL |
Family and domain databases
Gene3Di | 1.10.1040.10, 3 hits |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR013328, 6PGD_dom2 IPR013023, KARI IPR000506, KARI_C IPR013116, KARI_N IPR016207, KetolA_reductoisomerase_fun IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR21371, PTHR21371, 1 hit |
Pfami | View protein in Pfam PF01450, IlvC, 1 hit PF07991, IlvN, 1 hit |
PIRSFi | PIRSF000119, Ilv5_fungal, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00465, ilvC, 1 hit |
PROSITEi | View protein in PROSITE PS51851, KARI_C, 1 hit PS51850, KARI_N, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P06168-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRTQAARLI CNSRVITAKR TFALATRAAA YSRPAARFVK PMITTRGLKQ
60 70 80 90 100
INFGGTVETV YERADWPREK LLDYFKNDTF ALIGYGSQGY GQGLNLRDNG
110 120 130 140 150
LNVIIGVRKD GASWKAAIED GWVPGKNLFT VEDAIKRGSY VMNLLSDAAQ
160 170 180 190 200
SETWPAIKPL LTKGKTLYFS HGFSPVFKDL THVEPPKDLD VILVAPKGSG
210 220 230 240 250
RTVRSLFKEG RGINSSYAVW NDVTGKAHEK AQALAVAIGS GYVYQTTFER
260 270 280 290 300
EVNSDLYGER GCLMGGIHGM FLAQYDVLRE NGHSPSEAFN ETVEEATQSL
310 320 330 340 350
YPLIGKYGMD YMYDACSTTA RRGALDWYPI FKNALKPVFQ DLYESTKNGT
360 370 380 390
ETKRSLEFNS QPDYREKLEK ELDTIRNMEI WKVGKEVRKL RPENQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04969 Genomic DNA Translation: CAA28643.1 U19102 Genomic DNA Translation: AAB67753.1 BK006945 Genomic DNA Translation: DAA09659.1 |
PIRi | A24709 |
RefSeqi | NP_013459.1, NM_001182244.1 |
Genome annotation databases
EnsemblFungii | YLR355C_mRNA; YLR355C; YLR355C |
GeneIDi | 851069 |
KEGGi | sce:YLR355C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04969 Genomic DNA Translation: CAA28643.1 U19102 Genomic DNA Translation: AAB67753.1 BK006945 Genomic DNA Translation: DAA09659.1 |
PIRi | A24709 |
RefSeqi | NP_013459.1, NM_001182244.1 |
3D structure databases
SMRi | P06168 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31617, 133 interactors |
DIPi | DIP-6463N |
IntActi | P06168, 38 interactors |
MINTi | P06168 |
STRINGi | 4932.YLR355C |
Protein family/group databases
MoonProti | P06168 |
PTM databases
CarbonylDBi | P06168 |
iPTMneti | P06168 |
2D gel databases
SWISS-2DPAGEi | P06168 |
Proteomic databases
MaxQBi | P06168 |
PaxDbi | P06168 |
PRIDEi | P06168 |
TopDownProteomicsi | P06168 |
Genome annotation databases
EnsemblFungii | YLR355C_mRNA; YLR355C; YLR355C |
GeneIDi | 851069 |
KEGGi | sce:YLR355C |
Organism-specific databases
SGDi | S000004347, ILV5 |
VEuPathDBi | FungiDB:YLR355C |
Phylogenomic databases
eggNOGi | ENOG502QQBF, Eukaryota |
HOGENOMi | CLU_033821_1_2_1 |
InParanoidi | P06168 |
OMAi | RAMFSWL |
Enzyme and pathway databases
UniPathwayi | UPA00047;UER00056 UPA00049;UER00060 |
Miscellaneous databases
PROi | PR:P06168 |
RNActi | P06168, protein |
Family and domain databases
Gene3Di | 1.10.1040.10, 3 hits |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR013328, 6PGD_dom2 IPR013023, KARI IPR000506, KARI_C IPR013116, KARI_N IPR016207, KetolA_reductoisomerase_fun IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR21371, PTHR21371, 1 hit |
Pfami | View protein in Pfam PF01450, IlvC, 1 hit PF07991, IlvN, 1 hit |
PIRSFi | PIRSF000119, Ilv5_fungal, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00465, ilvC, 1 hit |
PROSITEi | View protein in PROSITE PS51851, KARI_C, 1 hit PS51850, KARI_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ILV5_YEAST | |
Accessioni | P06168Primary (citable) accession number: P06168 Secondary accession number(s): D6VYZ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | February 10, 2021 | |
This is version 200 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families