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Protein

L-lactate dehydrogenase A chain

Gene

Ldha

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase C chain (Ldhc), L-lactate dehydrogenase A chain (Ldha), L-lactate dehydrogenase B chain (Ldhb), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldhb), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldhb), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldhal6b), L-lactate dehydrogenase (Ldha)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99NADBy similarity1
Binding sitei106SubstrateBy similarity1
Binding sitei138NAD or substrateBy similarity1
Binding sitei169SubstrateBy similarity1
Active sitei193Proton acceptorBy similarity1
Binding sitei248SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 57NADBy similarityAdd BLAST29

GO - Molecular functioni

  • lactate dehydrogenase activity Source: MGI
  • L-lactate dehydrogenase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

ReactomeiR-MMU-70268 Pyruvate metabolism
UniPathwayi
UPA00554;UER00611

Protein family/group databases

MoonProtiP06151

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase A chain (EC:1.1.1.27)
Short name:
LDH-A
Alternative name(s):
LDH muscle subunit
Short name:
LDH-M
Gene namesi
Name:Ldha
Synonyms:Ldh-1, Ldh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96759 Ldha

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001684142 – 332L-lactate dehydrogenase A chainAdd BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei5N6-acetyllysine; alternateCombined sources1
Modified residuei5N6-succinyllysine; alternateCombined sources1
Modified residuei14N6-acetyllysineBy similarity1
Modified residuei57N6-acetyllysine; alternateBy similarity1
Cross-linki57Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei81N6-acetyllysineCombined sources1
Modified residuei118N6-acetyllysine; alternateCombined sources1
Modified residuei118N6-succinyllysine; alternateCombined sources1
Modified residuei126N6-acetyllysineCombined sources1
Modified residuei224N6-acetyllysineCombined sources1
Modified residuei232N6-acetyllysineCombined sources1
Modified residuei239PhosphotyrosineCombined sources1
Modified residuei243N6-acetyllysineCombined sources1
Modified residuei309PhosphothreonineBy similarity1
Modified residuei318N6-acetyllysine; alternateCombined sources1
Modified residuei318N6-succinyllysine; alternateCombined sources1
Modified residuei322PhosphothreonineBy similarity1

Post-translational modificationi

ISGylated.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP06151
PaxDbiP06151
PeptideAtlasiP06151
PRIDEiP06151
TopDownProteomicsiP06151

2D gel databases

REPRODUCTION-2DPAGEiP06151
SWISS-2DPAGEiP06151
UCD-2DPAGEiP06151

PTM databases

iPTMnetiP06151
PhosphoSitePlusiP06151
SwissPalmiP06151

Expressioni

Gene expression databases

BgeeiENSMUSG00000063229 Expressed in 320 organ(s), highest expression level in ear vesicle
CleanExiMM_LDHA
ExpressionAtlasiP06151 baseline and differential
GenevisibleiP06151 MM

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
HnrnpdQ606682EBI-444940,EBI-299932

Protein-protein interaction databases

BioGridi201127, 10 interactors
IntActiP06151, 22 interactors
MINTiP06151
STRINGi10090.ENSMUSP00000103267

Chemistry databases

BindingDBiP06151

Structurei

3D structure databases

ProteinModelPortaliP06151
SMRiP06151
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495 Eukaryota
COG0039 LUCA
GeneTreeiENSGT00550000074541
HOGENOMiHOG000213793
HOVERGENiHBG000462
InParanoidiP06151
KOiK00016
PhylomeDBiP06151

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 11 potential isoforms that are computationally mapped.Show allAlign All

P06151-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL
60 70 80 90 100
VDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ
110 120 130 140 150
QEGESRLNLV QRNVNIFKFI IPNIVKYSPH CKLLIVSNPV DILTYVAWKI
160 170 180 190 200
SGFPKNRVIG SGCNLDSARF RYLMGERLGV HALSCHGWVL GEHGDSSVPV
210 220 230 240 250
WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE VIKLKGYTSW
260 270 280 290 300
AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI
310 320 330
SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QF
Length:332
Mass (Da):36,499
Last modified:January 23, 2007 - v3
Checksum:i5AEB41E1E0B95100
GO

Computationally mapped potential isoform sequencesi

There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1B0GSX0A0A1B0GSX0_MOUSE
L-lactate dehydrogenase
Ldha mCG_19938
361Annotation score:
Q564E2Q564E2_MOUSE
L-lactate dehydrogenase
Ldha Ldh1, mCG_19938
332Annotation score:
A0A1B0GSR9A0A1B0GSR9_MOUSE
L-lactate dehydrogenase
Ldha
315Annotation score:
D3YZQ9D3YZQ9_MOUSE
L-lactate dehydrogenase
Ldha
236Annotation score:
A0A1B0GQX5A0A1B0GQX5_MOUSE
L-lactate dehydrogenase
Ldha
201Annotation score:
A0A1B0GT41A0A1B0GT41_MOUSE
L-lactate dehydrogenase A chain
Ldha
176Annotation score:
A0A1B0GRS2A0A1B0GRS2_MOUSE
L-lactate dehydrogenase A chain
Ldha
68Annotation score:
A0A1B0GSL7A0A1B0GSL7_MOUSE
L-lactate dehydrogenase A chain
Ldha
128Annotation score:
A0A1B0GRC1A0A1B0GRC1_MOUSE
L-lactate dehydrogenase A chain
Ldha
66Annotation score:
A0A1B0GS79A0A1B0GS79_MOUSE
L-lactate dehydrogenase A chain
Ldha
205Annotation score:
There is more potential isoformShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11N → I (PubMed:3996406).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00309 Genomic DNA Translation: CAA68410.1
X02520
, X02521, X02522, X02523, X02524, X02525, X02526 Genomic DNA Translation: CAA26360.1
U13687 mRNA Translation: AAA21466.1
X03753 Genomic DNA Translation: CAA27387.1
M17516 mRNA Translation: AAA39424.1
CCDSiCCDS21289.1
PIRiA25205 DEMSLM
I48240
RefSeqiNP_034829.1, NM_010699.2
UniGeneiMm.29324

Genome annotation databases

EnsembliENSMUST00000005051; ENSMUSP00000103267; ENSMUSG00000063229
ENSMUST00000048209; ENSMUSP00000036386; ENSMUSG00000063229
GeneIDi16828
KEGGimmu:16828
UCSCiuc009gzm.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00309 Genomic DNA Translation: CAA68410.1
X02520
, X02521, X02522, X02523, X02524, X02525, X02526 Genomic DNA Translation: CAA26360.1
U13687 mRNA Translation: AAA21466.1
X03753 Genomic DNA Translation: CAA27387.1
M17516 mRNA Translation: AAA39424.1
CCDSiCCDS21289.1
PIRiA25205 DEMSLM
I48240
RefSeqiNP_034829.1, NM_010699.2
UniGeneiMm.29324

3D structure databases

ProteinModelPortaliP06151
SMRiP06151
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201127, 10 interactors
IntActiP06151, 22 interactors
MINTiP06151
STRINGi10090.ENSMUSP00000103267

Chemistry databases

BindingDBiP06151

Protein family/group databases

MoonProtiP06151

PTM databases

iPTMnetiP06151
PhosphoSitePlusiP06151
SwissPalmiP06151

2D gel databases

REPRODUCTION-2DPAGEiP06151
SWISS-2DPAGEiP06151
UCD-2DPAGEiP06151

Proteomic databases

EPDiP06151
PaxDbiP06151
PeptideAtlasiP06151
PRIDEiP06151
TopDownProteomicsiP06151

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005051; ENSMUSP00000103267; ENSMUSG00000063229
ENSMUST00000048209; ENSMUSP00000036386; ENSMUSG00000063229
GeneIDi16828
KEGGimmu:16828
UCSCiuc009gzm.2 mouse

Organism-specific databases

CTDi3939
MGIiMGI:96759 Ldha

Phylogenomic databases

eggNOGiKOG1495 Eukaryota
COG0039 LUCA
GeneTreeiENSGT00550000074541
HOGENOMiHOG000213793
HOVERGENiHBG000462
InParanoidiP06151
KOiK00016
PhylomeDBiP06151

Enzyme and pathway databases

UniPathwayi
UPA00554;UER00611

ReactomeiR-MMU-70268 Pyruvate metabolism

Miscellaneous databases

ChiTaRSiLdha mouse
PROiPR:P06151
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000063229 Expressed in 320 organ(s), highest expression level in ear vesicle
CleanExiMM_LDHA
ExpressionAtlasiP06151 baseline and differential
GenevisibleiP06151 MM

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLDHA_MOUSE
AccessioniPrimary (citable) accession number: P06151
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 184 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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