UniProtKB - P06133 (UD2B4_HUMAN)
Protein
UDP-glucuronosyltransferase 2B4
Gene
UGT2B4
Organism
Homo sapiens (Human)
Status
Functioni
UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed:18719240, PubMed:23288867). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed:18719240, PubMed:23288867). Catalyzes the glucuronidation of the endogenous estrogen hormones such as estradiol and estriol (PubMed:18719240, PubMed:23288867).2 Publications
Caution
UGT2B4 has been previously described as an enzyme named UGT2B11. However the name UGT2B11 has now been reused for another human protein (see AC O75310).Curated
Catalytic activityi
- EC:2.4.1.172 PublicationsThis reaction proceeds in the forward2 Publications direction.
- This reaction proceeds in the forward1 Publication direction.
- 16α,17α-estriol + UDP-α-D-glucuronate = 16α,17α-estriol 17-O-(β-D-glucuronate) + H+ + UDP1 PublicationThis reaction proceeds in the forward1 Publication direction.
Kineticsi
Some kinetic parameters were assessed using commercial enzymes, which may represent a mix of both active and inactive protein forms, and therefore modify the kinetic values.2 Publications
- KM=4.77 µM for 17alpha-estradiol/epiestradiol (when assaying glucuronidation at position 17)1 Publication
- KM=57.8 µM for calcitriol (when assaying glucuronidation at position 25)1 Publication
- Vmax=135 pmol/min/mg enzyme for the formation of 17alpha-estradiol 17-O-(beta-D-glucuronate)1 Publication
- Vmax=17 pmol/min/mg enzyme for the formation of 17alpha-estradiol 17-O-(beta-D-glucuronate)1 Publication
- Vmax=3.8 pmol/min/mg enzyme for the formation of 16alpha,17beta-estriol 16-O-(beta-D-glucuronate)1 Publication
- Vmax=9.2 pmol/min/mg enzyme for the formation of 16beta,17beta-estriol 16-O-(beta-D-glucuronate)1 Publication
- Vmax=174 pmol/min/mg enzyme for the formation of 16alpha,17alpha-estriol 17-O-(beta-D-glucuronate)1 Publication
- Vmax=4.3 pmol/min/mg enzyme for the formation of calcitriol 25-O-(beta-D-glucuronate)1 Publication
GO - Molecular functioni
- glucuronosyltransferase activity Source: UniProtKB
GO - Biological processi
- cellular glucuronidation Source: UniProtKB
- estrogen metabolic process Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.4.1.17, 2681 |
PathwayCommonsi | P06133 |
Reactomei | R-HSA-156588, Glucuronidation |
Protein family/group databases
CAZyi | GT1, Glycosyltransferase Family 1 |
Chemistry databases
SwissLipidsi | SLP:000001708 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-glucuronosyltransferase 2B41 Publication (EC:2.4.1.171 Publication)Short name: UDPGT 2B4 Short name: UGT2B4 Alternative name(s): HLUG25 Hyodeoxycholic acid-specific UDPGT UDPGTh-1 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12553, UGT2B4 |
MIMi | 600067, gene |
neXtProti | NX_P06133 |
VEuPathDBi | HostDB:ENSG00000156096.12 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 493 – 509 | HelicalSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 7363 |
OpenTargetsi | ENSG00000156096 |
PharmGKBi | PA360 |
Miscellaneous databases
Pharosi | P06133, Tbio |
Chemistry databases
ChEMBLi | CHEMBL6196 |
DrugBanki | DB00714, Apomorphine DB08907, Canagliflozin DB01136, Carvedilol DB06119, Cenobamate DB00318, Codeine DB14635, Curcumin sulfate DB06292, Dapagliflozin DB09213, Dexibuprofen DB00514, Dextromethorphan DB00586, Diclofenac DB00783, Estradiol DB00712, Flurbiprofen DB11796, Fostemsavir DB06741, Gavestinel DB01241, Gemfibrozil DB01050, Ibuprofen DB00555, Lamotrigine DB00295, Morphine DB00794, Primidone DB00503, Ritonavir |
Genetic variation databases
BioMutai | UGT2B4 |
DMDMi | 6175083 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | By similarityAdd BLAST | 23 | |
ChainiPRO_0000036028 | 24 – 528 | UDP-glucuronosyltransferase 2B4Add BLAST | 505 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 315 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
jPOSTi | P06133 |
MassIVEi | P06133 |
MaxQBi | P06133 |
PaxDbi | P06133 |
PeptideAtlasi | P06133 |
PRIDEi | P06133 |
ProteomicsDBi | 34076 51871 [P06133-1] 850 |
PTM databases
GlyGeni | P06133, 1 site |
iPTMneti | P06133 |
PhosphoSitePlusi | P06133 |
Expressioni
Gene expression databases
Bgeei | ENSG00000156096, Expressed in intestine and 67 other tissues |
ExpressionAtlasi | P06133, baseline and differential |
Genevisiblei | P06133, HS |
Organism-specific databases
HPAi | ENSG00000156096, Tissue enriched (liver) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 113210, 1 interactor |
IntActi | P06133, 1 interactor |
STRINGi | 9606.ENSP00000305221 |
Miscellaneous databases
RNActi | P06133, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the UDP-glycosyltransferase family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1192, Eukaryota |
GeneTreei | ENSGT00940000158332 |
HOGENOMi | CLU_012949_5_1_1 |
InParanoidi | P06133 |
OMAi | IYVLYFE |
OrthoDBi | 508327at2759 |
PhylomeDBi | P06133 |
TreeFami | TF315472 |
Family and domain databases
InterProi | View protein in InterPro IPR002213, UDP_glucos_trans IPR035595, UDP_glycos_trans_CS |
Pfami | View protein in Pfam PF00201, UDPGT, 1 hit |
PROSITEi | View protein in PROSITE PS00375, UDPGT, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P06133-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSMKWTSALL LIQLSCYFSS GSCGKVLVWP TEFSHWMNIK TILDELVQRG
60 70 80 90 100
HEVTVLASSA SISFDPNSPS TLKFEVYPVS LTKTEFEDII KQLVKRWAEL
110 120 130 140 150
PKDTFWSYFS QVQEIMWTFN DILRKFCKDI VSNKKLMKKL QESRFDVVLA
160 170 180 190 200
DAVFPFGELL AELLKIPFVY SLRFSPGYAI EKHSGGLLFP PSYVPVVMSE
210 220 230 240 250
LSDQMTFIER VKNMIYVLYF EFWFQIFDMK KWDQFYSEVL GRPTTLSETM
260 270 280 290 300
AKADIWLIRN YWDFQFPHPL LPNVEFVGGL HCKPAKPLPK EMEEFVQSSG
310 320 330 340 350
ENGVVVFSLG SMVSNTSEER ANVIASALAK IPQKVLWRFD GNKPDTLGLN
360 370 380 390 400
TRLYKWIPQN DLLGHPKTRA FITHGGANGI YEAIYHGIPM VGVPLFADQP
410 420 430 440 450
DNIAHMKAKG AAVSLDFHTM SSTDLLNALK TVINDPLYKE NAMKLSRIHH
460 470 480 490 500
DQPVKPLDRA VFWIEFVMRH KGAKHLRVAA HDLTWFQYHS LDVTGFLLAC
510 520
VATVIFIITK CLFCVWKFVR TGKKGKRD
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RGY0 | D6RGY0_HUMAN | UDP-glucuronosyltransferase 2B4 | UGT2B4 | 54 | Annotation score: | ||
A0A1W2PQJ2 | A0A1W2PQJ2_HUMAN | UDP-glucuronosyltransferase 2B4 | UGT2B4 | 369 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 171 – 172 | SL → RP in CAA68415 (PubMed:3109396).Curated | 2 | |
Sequence conflicti | 291 – 293 | EME → KWK in AAC32272 (Ref. 4) Curated | 3 | |
Sequence conflicti | 309 | L → V in AAS47487 (Ref. 6) 1 Publication | 1 | |
Sequence conflicti | 354 | Y → H in AAS47487 (Ref. 6) 1 Publication | 1 | |
Sequence conflicti | 382 – 387 | EAIYHG → KAISPR in CAA68415 (PubMed:3109396).Curated | 6 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_060713 | 40 | K → N1 PublicationCorresponds to variant dbSNP:rs41299974EnsemblClinVar. | 1 | |
Natural variantiVAR_060714 | 78 | P → T1 PublicationCorresponds to variant dbSNP:rs41299976Ensembl. | 1 | |
Natural variantiVAR_060715 | 80 | S → P1 PublicationCorresponds to variant dbSNP:rs41299978EnsemblClinVar. | 1 | |
Natural variantiVAR_011328 | 109 | F → L1 Publication | 1 | |
Natural variantiVAR_060716 | 277 | V → I1 PublicationCorresponds to variant dbSNP:rs41300004Ensembl. | 1 | |
Natural variantiVAR_011329 | 396 | F → L1 PublicationCorresponds to variant dbSNP:rs72552707Ensembl. | 1 | |
Natural variantiVAR_007712 | 458 | D → E2 PublicationsCorresponds to variant dbSNP:rs13119049Ensembl. | 1 | |
Natural variantiVAR_060717 | 511 | C → R1 PublicationCorresponds to variant dbSNP:rs41298245Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056679 | 1 – 14 | MSMKW…LLIQL → MFFALLHVSSTNGL in isoform 2. 1 PublicationAdd BLAST | 14 | |
Alternative sequenceiVSP_056680 | 15 – 150 | Missing in isoform 2. 1 PublicationAdd BLAST | 136 | |
Alternative sequenceiVSP_056869 | 364 – 369 | GHPKTR → DIKRML in isoform 3. 1 Publication | 6 | |
Alternative sequenceiVSP_056870 | 370 – 528 | Missing in isoform 3. 1 PublicationAdd BLAST | 159 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00317 mRNA Translation: CAA68415.1 AF064200 mRNA Translation: AAC95002.1 AJ005162 mRNA Translation: CAA06396.1 AF081793 mRNA Translation: AAC32272.1 AF135416 Genomic DNA Translation: AAF78145.1 AY529122 mRNA Translation: AAS47487.1 DQ520733 Genomic DNA Translation: ABF47107.1 AK292748 mRNA Translation: BAF85437.1 AK300084 mRNA Translation: BAG61887.1 AC093829 Genomic DNA No translation available. AC108078 Genomic DNA No translation available. CH471057 Genomic DNA Translation: EAX05591.1 BC026264 mRNA Translation: AAH26264.1 |
CCDSi | CCDS43234.1 [P06133-1] CCDS75137.1 [P06133-3] |
PIRi | JN0619 |
RefSeqi | NP_001284544.1, NM_001297615.1 [P06133-3] NP_001284545.1, NM_001297616.1 [P06133-2] NP_066962.2, NM_021139.2 [P06133-1] |
Genome annotation databases
Ensembli | ENST00000305107; ENSP00000305221; ENSG00000156096 [P06133-1] ENST00000512583; ENSP00000421290; ENSG00000156096 [P06133-3] |
GeneIDi | 7363 |
KEGGi | hsa:7363 |
UCSCi | uc003hek.4, human [P06133-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00317 mRNA Translation: CAA68415.1 AF064200 mRNA Translation: AAC95002.1 AJ005162 mRNA Translation: CAA06396.1 AF081793 mRNA Translation: AAC32272.1 AF135416 Genomic DNA Translation: AAF78145.1 AY529122 mRNA Translation: AAS47487.1 DQ520733 Genomic DNA Translation: ABF47107.1 AK292748 mRNA Translation: BAF85437.1 AK300084 mRNA Translation: BAG61887.1 AC093829 Genomic DNA No translation available. AC108078 Genomic DNA No translation available. CH471057 Genomic DNA Translation: EAX05591.1 BC026264 mRNA Translation: AAH26264.1 |
CCDSi | CCDS43234.1 [P06133-1] CCDS75137.1 [P06133-3] |
PIRi | JN0619 |
RefSeqi | NP_001284544.1, NM_001297615.1 [P06133-3] NP_001284545.1, NM_001297616.1 [P06133-2] NP_066962.2, NM_021139.2 [P06133-1] |
3D structure databases
SMRi | P06133 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 113210, 1 interactor |
IntActi | P06133, 1 interactor |
STRINGi | 9606.ENSP00000305221 |
Chemistry databases
ChEMBLi | CHEMBL6196 |
DrugBanki | DB00714, Apomorphine DB08907, Canagliflozin DB01136, Carvedilol DB06119, Cenobamate DB00318, Codeine DB14635, Curcumin sulfate DB06292, Dapagliflozin DB09213, Dexibuprofen DB00514, Dextromethorphan DB00586, Diclofenac DB00783, Estradiol DB00712, Flurbiprofen DB11796, Fostemsavir DB06741, Gavestinel DB01241, Gemfibrozil DB01050, Ibuprofen DB00555, Lamotrigine DB00295, Morphine DB00794, Primidone DB00503, Ritonavir |
SwissLipidsi | SLP:000001708 |
Protein family/group databases
CAZyi | GT1, Glycosyltransferase Family 1 |
PTM databases
GlyGeni | P06133, 1 site |
iPTMneti | P06133 |
PhosphoSitePlusi | P06133 |
Genetic variation databases
BioMutai | UGT2B4 |
DMDMi | 6175083 |
Proteomic databases
jPOSTi | P06133 |
MassIVEi | P06133 |
MaxQBi | P06133 |
PaxDbi | P06133 |
PeptideAtlasi | P06133 |
PRIDEi | P06133 |
ProteomicsDBi | 34076 51871 [P06133-1] 850 |
Protocols and materials databases
Antibodypediai | 24236, 196 antibodies |
DNASUi | 7363 |
Genome annotation databases
Ensembli | ENST00000305107; ENSP00000305221; ENSG00000156096 [P06133-1] ENST00000512583; ENSP00000421290; ENSG00000156096 [P06133-3] |
GeneIDi | 7363 |
KEGGi | hsa:7363 |
UCSCi | uc003hek.4, human [P06133-1] |
Organism-specific databases
CTDi | 7363 |
DisGeNETi | 7363 |
GeneCardsi | UGT2B4 |
HGNCi | HGNC:12553, UGT2B4 |
HPAi | ENSG00000156096, Tissue enriched (liver) |
MIMi | 600067, gene |
neXtProti | NX_P06133 |
OpenTargetsi | ENSG00000156096 |
PharmGKBi | PA360 |
VEuPathDBi | HostDB:ENSG00000156096.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1192, Eukaryota |
GeneTreei | ENSGT00940000158332 |
HOGENOMi | CLU_012949_5_1_1 |
InParanoidi | P06133 |
OMAi | IYVLYFE |
OrthoDBi | 508327at2759 |
PhylomeDBi | P06133 |
TreeFami | TF315472 |
Enzyme and pathway databases
BRENDAi | 2.4.1.17, 2681 |
PathwayCommonsi | P06133 |
Reactomei | R-HSA-156588, Glucuronidation |
Miscellaneous databases
BioGRID-ORCSi | 7363, 3 hits in 984 CRISPR screens |
ChiTaRSi | UGT2B4, human |
GeneWikii | UGT2B4 |
GenomeRNAii | 7363 |
Pharosi | P06133, Tbio |
PROi | PR:P06133 |
RNActi | P06133, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000156096, Expressed in intestine and 67 other tissues |
ExpressionAtlasi | P06133, baseline and differential |
Genevisiblei | P06133, HS |
Family and domain databases
InterProi | View protein in InterPro IPR002213, UDP_glucos_trans IPR035595, UDP_glycos_trans_CS |
Pfami | View protein in Pfam PF00201, UDPGT, 1 hit |
PROSITEi | View protein in PROSITE PS00375, UDPGT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UD2B4_HUMAN | |
Accessioni | P06133Primary (citable) accession number: P06133 Secondary accession number(s): A6NCP7 Q6QQX7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | December 15, 1998 | |
Last modified: | April 7, 2021 | |
This is version 197 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families