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Protein

General negative regulator of transcription subunit 3

Gene

NOT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

Miscellaneous

Present with 2490 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be CDC39 (which is in fact NOT1).1 Publication

GO - Biological processi

  • deadenylation-dependent decapping of nuclear-transcribed mRNA Source: SGD
  • negative regulation of translation Source: GO_Central
  • nuclear-transcribed mRNA poly(A) tail shortening Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • protein ubiquitination Source: SGD
  • regulation of transcription by RNA polymerase II Source: SGD

Keywordsi

Molecular functionActivator, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31310-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
General negative regulator of transcription subunit 3
Gene namesi
Name:NOT3
Ordered Locus Names:YIL038C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

SGDiS000001300 NOT3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001983371 – 836General negative regulator of transcription subunit 3Add BLAST836

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei303PhosphoserineCombined sources1
Modified residuei307PhosphoserineCombined sources1
Modified residuei322PhosphoserineCombined sources1
Modified residuei446PhosphoserineCombined sources1
Modified residuei450PhosphoserineCombined sources1
Cross-linki535Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei565PhosphoserineCombined sources1
Modified residuei569PhosphoserineCombined sources1
Modified residuei571PhosphothreonineCombined sources1
Modified residuei657PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP06102
PaxDbiP06102
PRIDEiP06102
TopDownProteomicsiP06102

PTM databases

iPTMnetiP06102

Interactioni

Subunit structurei

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi34952, 249 interactors
ComplexPortaliCPX-1800 Ccr4-Not complex
DIPiDIP-2256N
IntActiP06102, 45 interactors
MINTiP06102
STRINGi4932.YIL038C

Structurei

3D structure databases

ProteinModelPortaliP06102
SMRiP06102
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili36 – 68Sequence analysisAdd BLAST33
Coiled coili119 – 195Sequence analysisAdd BLAST77
Coiled coili255 – 292Sequence analysisAdd BLAST38
Coiled coili803 – 831Sequence analysisAdd BLAST29

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000014743
HOGENOMiHOG000113831
InParanoidiP06102
KOiK12580
OMAiYVYFDWE
OrthoDBiEOG092C3ROC

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR012270 CCR4-NOT_su3/5
IPR007282 NOT2/3/5_C
IPR007207 Not_N
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit
PF04065 Not3, 1 hit
PIRSFiPIRSF005290 NOT_su_3_5, 1 hit

Sequencei

Sequence statusi: Complete.

P06102-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAHRKLQQEV DRVFKKINEG LEIFNSYYER HESCTNNPSQ KDKLESDLKR
60 70 80 90 100
EVKKLQRLRE QIKSWQSSPD IKDKDSLLDY RRSVEIAMEK YKAVEKASKE
110 120 130 140 150
KAYSNISLKK SETLDPQERE RRDISEYLSQ MIDELERQYD SLQVEIDKLL
160 170 180 190 200
LLNKKKKTSS TTNDEKKEQY KRFQARYRWH QQQMELALRL LANEELDPQD
210 220 230 240 250
VKNVQDDINY FVESNQDPDF VEDETIYDGL NLQSNEAIAH EVAQYFASQN
260 270 280 290 300
AEDNNTSDAN ESLQDISKLS KKEQRKLERE AKKAAKLAAK NATGAAIPVA
310 320 330 340 350
GPSSTPSPVI PVADASKETE RSPSSSPIHN ATKPEEAVKT SIKSPRSSAD
360 370 380 390 400
NLLPSLQKSP SSATPETPTN VHTHIHQTPN GITGATTLKP ATLPAKPAGE
410 420 430 440 450
LKWAVAASQA VEKDRKVTSA SSTISNTSTK TPTTAAATTT SSNANSRIGS
460 470 480 490 500
ALNTPKLSTS SLSLQPDNTG ASSSAATAAA VLAAGAAAVH QNNQAFYRNM
510 520 530 540 550
SSSHHPLVSL ATNPKSEHEV ATTVNQNGPE NTTKKVMEQK EEESPEERNK
560 570 580 590 600
LQVPTFGVFD DDFESDRDSE TEPEEEEQPS TPKYLSLEQR EAKTNEIKKE
610 620 630 640 650
FVSDFETLLL PSGVQEFIMS SELYNSQIES KITYKRSRDM CEISRLVEVP
660 670 680 690 700
QGVNPPSPLD AFRSTQQWDV MRCSLRDIII GSERLKEDSS SIYAKILENF
710 720 730 740 750
RTLEMFSLFY NYYFAITPLE REIAYKILNE RDWKVSKDGT MWFLRQGEVK
760 770 780 790 800
FFNEICEVGD YKIFKLDDWT VIDKINFRLD YSFLQPPVDT ASEVRDVSVD
810 820 830
NNNVNDQSNV TLEQQKQEIS HGKQLLKQLK QGKISV
Length:836
Mass (Da):94,403
Last modified:February 1, 1995 - v2
Checksum:i69B76694FCC6846F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106I → N in CAA86913 (PubMed:3018676).Curated1
Sequence conflicti593K → R in AAT92916 (PubMed:17322287).Curated1
Sequence conflicti725Y → C in CAA86913 (PubMed:3018676).Curated1
Sequence conflicti827 – 836KQLKQGKISV → ETIETGKN in CAA86913 (PubMed:3018676).Curated10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46861 Genomic DNA Translation: CAA86913.1
X04289 Genomic DNA Translation: CAA27837.1
AY692897 Genomic DNA Translation: AAT92916.1
BK006942 Genomic DNA Translation: DAA08510.1
PIRiS49940
RefSeqiNP_012226.3, NM_001179388.3

Genome annotation databases

EnsemblFungiiYIL038C; YIL038C; YIL038C
GeneIDi854773
KEGGisce:YIL038C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46861 Genomic DNA Translation: CAA86913.1
X04289 Genomic DNA Translation: CAA27837.1
AY692897 Genomic DNA Translation: AAT92916.1
BK006942 Genomic DNA Translation: DAA08510.1
PIRiS49940
RefSeqiNP_012226.3, NM_001179388.3

3D structure databases

ProteinModelPortaliP06102
SMRiP06102
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34952, 249 interactors
ComplexPortaliCPX-1800 Ccr4-Not complex
DIPiDIP-2256N
IntActiP06102, 45 interactors
MINTiP06102
STRINGi4932.YIL038C

PTM databases

iPTMnetiP06102

Proteomic databases

MaxQBiP06102
PaxDbiP06102
PRIDEiP06102
TopDownProteomicsiP06102

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL038C; YIL038C; YIL038C
GeneIDi854773
KEGGisce:YIL038C

Organism-specific databases

SGDiS000001300 NOT3

Phylogenomic databases

GeneTreeiENSGT00390000014743
HOGENOMiHOG000113831
InParanoidiP06102
KOiK12580
OMAiYVYFDWE
OrthoDBiEOG092C3ROC

Enzyme and pathway databases

BioCyciYEAST:G3O-31310-MONOMER

Miscellaneous databases

PROiPR:P06102

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR012270 CCR4-NOT_su3/5
IPR007282 NOT2/3/5_C
IPR007207 Not_N
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit
PF04065 Not3, 1 hit
PIRSFiPIRSF005290 NOT_su_3_5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNOT3_YEAST
AccessioniPrimary (citable) accession number: P06102
Secondary accession number(s): D6VVP4, Q6B233
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: February 1, 1995
Last modified: November 7, 2018
This is version 179 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names
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Main funding by: National Institutes of Health

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