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Entry version 182 (13 Feb 2019)
Sequence version 2 (01 Feb 1995)
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Protein

General negative regulator of transcription subunit 3

Gene

NOT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

Miscellaneous

Present with 2490 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be CDC39 (which is in fact NOT1).1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-31310-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
General negative regulator of transcription subunit 3
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:NOT3
Ordered Locus Names:YIL038C
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome IX

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YIL038C

Saccharomyces Genome Database

More...
SGDi
S000001300 NOT3

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001983371 – 836General negative regulator of transcription subunit 3Add BLAST836

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei303PhosphoserineCombined sources1
Modified residuei307PhosphoserineCombined sources1
Modified residuei322PhosphoserineCombined sources1
Modified residuei446PhosphoserineCombined sources1
Modified residuei450PhosphoserineCombined sources1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki535Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei565PhosphoserineCombined sources1
Modified residuei569PhosphoserineCombined sources1
Modified residuei571PhosphothreonineCombined sources1
Modified residuei657PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P06102

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P06102

PRoteomics IDEntifications database

More...
PRIDEi
P06102

Consortium for Top Down Proteomics

More...
TopDownProteomicsi
P06102

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P06102

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.2 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
34952, 250 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-1800 Ccr4-Not complex

Database of interacting proteins

More...
DIPi
DIP-2256N

Protein interaction database and analysis system

More...
IntActi
P06102, 45 interactors

Molecular INTeraction database

More...
MINTi
P06102

STRING: functional protein association networks

More...
STRINGi
4932.YIL038C

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
P06102

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P06102

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili36 – 68Sequence analysisAdd BLAST33
Coiled coili119 – 195Sequence analysisAdd BLAST77
Coiled coili255 – 292Sequence analysisAdd BLAST38
Coiled coili803 – 831Sequence analysisAdd BLAST29

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000113831

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P06102

KEGG Orthology (KO)

More...
KOi
K12580

Identification of Orthologs from Complete Genome Data

More...
OMAi
YVYFDWE

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.30.30.1020, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR012270 CCR4-NOT_su3/5
IPR040168 Not2/3/5
IPR007282 NOT2/3/5_C
IPR007207 Not_N

The PANTHER Classification System

More...
PANTHERi
PTHR23326 PTHR23326, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04153 NOT2_3_5, 1 hit
PF04065 Not3, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF005290 NOT_su_3_5, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P06102-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAHRKLQQEV DRVFKKINEG LEIFNSYYER HESCTNNPSQ KDKLESDLKR
60 70 80 90 100
EVKKLQRLRE QIKSWQSSPD IKDKDSLLDY RRSVEIAMEK YKAVEKASKE
110 120 130 140 150
KAYSNISLKK SETLDPQERE RRDISEYLSQ MIDELERQYD SLQVEIDKLL
160 170 180 190 200
LLNKKKKTSS TTNDEKKEQY KRFQARYRWH QQQMELALRL LANEELDPQD
210 220 230 240 250
VKNVQDDINY FVESNQDPDF VEDETIYDGL NLQSNEAIAH EVAQYFASQN
260 270 280 290 300
AEDNNTSDAN ESLQDISKLS KKEQRKLERE AKKAAKLAAK NATGAAIPVA
310 320 330 340 350
GPSSTPSPVI PVADASKETE RSPSSSPIHN ATKPEEAVKT SIKSPRSSAD
360 370 380 390 400
NLLPSLQKSP SSATPETPTN VHTHIHQTPN GITGATTLKP ATLPAKPAGE
410 420 430 440 450
LKWAVAASQA VEKDRKVTSA SSTISNTSTK TPTTAAATTT SSNANSRIGS
460 470 480 490 500
ALNTPKLSTS SLSLQPDNTG ASSSAATAAA VLAAGAAAVH QNNQAFYRNM
510 520 530 540 550
SSSHHPLVSL ATNPKSEHEV ATTVNQNGPE NTTKKVMEQK EEESPEERNK
560 570 580 590 600
LQVPTFGVFD DDFESDRDSE TEPEEEEQPS TPKYLSLEQR EAKTNEIKKE
610 620 630 640 650
FVSDFETLLL PSGVQEFIMS SELYNSQIES KITYKRSRDM CEISRLVEVP
660 670 680 690 700
QGVNPPSPLD AFRSTQQWDV MRCSLRDIII GSERLKEDSS SIYAKILENF
710 720 730 740 750
RTLEMFSLFY NYYFAITPLE REIAYKILNE RDWKVSKDGT MWFLRQGEVK
760 770 780 790 800
FFNEICEVGD YKIFKLDDWT VIDKINFRLD YSFLQPPVDT ASEVRDVSVD
810 820 830
NNNVNDQSNV TLEQQKQEIS HGKQLLKQLK QGKISV
Length:836
Mass (Da):94,403
Last modified:February 1, 1995 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i69B76694FCC6846F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti106I → N in CAA86913 (PubMed:3018676).Curated1
Sequence conflicti593K → R in AAT92916 (PubMed:17322287).Curated1
Sequence conflicti725Y → C in CAA86913 (PubMed:3018676).Curated1
Sequence conflicti827 – 836KQLKQGKISV → ETIETGKN in CAA86913 (PubMed:3018676).Curated10

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Z46861 Genomic DNA Translation: CAA86913.1
X04289 Genomic DNA Translation: CAA27837.1
AY692897 Genomic DNA Translation: AAT92916.1
BK006942 Genomic DNA Translation: DAA08510.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S49940

NCBI Reference Sequences

More...
RefSeqi
NP_012226.3, NM_001179388.3

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YIL038C_mRNA; YIL038C_mRNA; YIL038C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
854773

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YIL038C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46861 Genomic DNA Translation: CAA86913.1
X04289 Genomic DNA Translation: CAA27837.1
AY692897 Genomic DNA Translation: AAT92916.1
BK006942 Genomic DNA Translation: DAA08510.1
PIRiS49940
RefSeqiNP_012226.3, NM_001179388.3

3D structure databases

ProteinModelPortaliP06102
SMRiP06102
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34952, 250 interactors
ComplexPortaliCPX-1800 Ccr4-Not complex
DIPiDIP-2256N
IntActiP06102, 45 interactors
MINTiP06102
STRINGi4932.YIL038C

PTM databases

iPTMnetiP06102

Proteomic databases

MaxQBiP06102
PaxDbiP06102
PRIDEiP06102
TopDownProteomicsiP06102

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL038C_mRNA; YIL038C_mRNA; YIL038C
GeneIDi854773
KEGGisce:YIL038C

Organism-specific databases

EuPathDBiFungiDB:YIL038C
SGDiS000001300 NOT3

Phylogenomic databases

HOGENOMiHOG000113831
InParanoidiP06102
KOiK12580
OMAiYVYFDWE

Enzyme and pathway databases

BioCyciYEAST:G3O-31310-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P06102

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR012270 CCR4-NOT_su3/5
IPR040168 Not2/3/5
IPR007282 NOT2/3/5_C
IPR007207 Not_N
PANTHERiPTHR23326 PTHR23326, 1 hit
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit
PF04065 Not3, 1 hit
PIRSFiPIRSF005290 NOT_su_3_5, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNOT3_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P06102
Secondary accession number(s): D6VVP4, Q6B233
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: February 1, 1995
Last modified: February 13, 2019
This is version 182 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names
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