NOT3

Functionⁱ

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

Miscellaneous

Present with 2490 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be CDC39 (which is in fact NOT1).1 Publication

GO - Biological processⁱ

deadenylation-dependent decapping of nuclear-transcribed mRNA
Source: SGD
Inferred from mutant phenotypeⁱ
- 26952104
negative regulation of translation Source: GO_Central
nuclear-transcribed mRNA poly(A) tail shortening
Source: SGD
Inferred from direct assayⁱ
- 11889048
positive regulation of transcription elongation from RNA polymerase II promoter
Source: SGD
Inferred from direct assayⁱ
- 21406554
protein ubiquitination
Source: SGD
Inferred from mutant phenotypeⁱ
- 16926149
regulation of transcription by RNA polymerase II
Source: SGD
Inferred from physical interactionⁱ
- 10490603
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, Repressor
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-31310-MONOMER

BioCycⁱ

YEAST:G3O-31310-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: General negative regulator of transcription subunit 3
Gene namesⁱ	Name:NOT3 Ordered Locus Names:YIL038C
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome IX

Organism-specific databases

EuPathDBⁱ	FungiDB:YIL038C
Saccharomyces Genome Database More...SGDⁱ	S000001300 NOT3

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000198337	1 – 836	General negative regulator of transcription subunit 3Add BLAST		836

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	303	PhosphoserineCombined sources	1
Modified residueⁱ	307	PhosphoserineCombined sources	1
Modified residueⁱ	322	PhosphoserineCombined sources	1
Modified residueⁱ	446	PhosphoserineCombined sources	1
Modified residueⁱ	450	PhosphoserineCombined sources	1
Cross-linkⁱ	535	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residueⁱ	565	PhosphoserineCombined sources	1
Modified residueⁱ	569	PhosphoserineCombined sources	1
Modified residueⁱ	571	PhosphothreonineCombined sources	1
Modified residueⁱ	657	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P06102
PaxDbⁱ	P06102
PRIDEⁱ	P06102
TopDownProteomicsⁱ	P06102

PTM databases

iPTMnetⁱ	P06102

iPTMnetⁱ

P06102

Interactionⁱ

Subunit structureⁱ

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CAF40	P53829	3	EBI-12165,EBI-28306
CDC36	P06100	2	EBI-12165,EBI-12153
CDC39	P25655	3	EBI-12165,EBI-12139

Protein-protein interaction databases

BioGridⁱ	34952, 249 interactors
ComplexPortalⁱ	CPX-1800 Ccr4-Not complex
Database of interacting proteins More...DIPⁱ	DIP-2256N
IntActⁱ	P06102, 45 interactors
Molecular INTeraction database More...MINTⁱ	P06102
STRINGⁱ	4932.YIL038C

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P06102
SMRⁱ	P06102
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Coiled coil

Feature key	Position(s)	DescriptionActions	Length
Coiled coilⁱ	36 – 68	Sequence analysisAdd BLAST	33
Coiled coilⁱ	119 – 195	Sequence analysisAdd BLAST	77
Coiled coilⁱ	255 – 292	Sequence analysisAdd BLAST	38
Coiled coilⁱ	803 – 831	Sequence analysisAdd BLAST	29

Sequence similaritiesⁱ

Belongs to the CNOT2/3/5 family.Curated

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000014743
HOGENOMⁱ	HOG000113831
InParanoidⁱ	P06102
KEGG Orthology (KO) More...KOⁱ	K12580
OMAⁱ	DYQVFDL
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C3ROC

Family and domain databases

Gene3Dⁱ	2.30.30.1020, 1 hit
InterProⁱ	View protein in InterPro IPR038635 CCR4-NOT_su2/3/5_N_sf IPR012270 CCR4-NOT_su3/5 IPR007282 NOT IPR007207 Not_N
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04153 NOT2_3_5, 1 hit PF04065 Not3, 1 hit
PIRSFⁱ	PIRSF005290 NOT_su_3_5, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P06102-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAHRKLQQEV DRVFKKINEG LEIFNSYYER HESCTNNPSQ KDKLESDLKR 
        60         70         80         90        100
EVKKLQRLRE QIKSWQSSPD IKDKDSLLDY RRSVEIAMEK YKAVEKASKE 
       110        120        130        140        150
KAYSNISLKK SETLDPQERE RRDISEYLSQ MIDELERQYD SLQVEIDKLL 
       160        170        180        190        200
LLNKKKKTSS TTNDEKKEQY KRFQARYRWH QQQMELALRL LANEELDPQD 
       210        220        230        240        250
VKNVQDDINY FVESNQDPDF VEDETIYDGL NLQSNEAIAH EVAQYFASQN 
       260        270        280        290        300
AEDNNTSDAN ESLQDISKLS KKEQRKLERE AKKAAKLAAK NATGAAIPVA 
       310        320        330        340        350
GPSSTPSPVI PVADASKETE RSPSSSPIHN ATKPEEAVKT SIKSPRSSAD 
       360        370        380        390        400
NLLPSLQKSP SSATPETPTN VHTHIHQTPN GITGATTLKP ATLPAKPAGE 
       410        420        430        440        450
LKWAVAASQA VEKDRKVTSA SSTISNTSTK TPTTAAATTT SSNANSRIGS 
       460        470        480        490        500
ALNTPKLSTS SLSLQPDNTG ASSSAATAAA VLAAGAAAVH QNNQAFYRNM 
       510        520        530        540        550
SSSHHPLVSL ATNPKSEHEV ATTVNQNGPE NTTKKVMEQK EEESPEERNK 
       560        570        580        590        600
LQVPTFGVFD DDFESDRDSE TEPEEEEQPS TPKYLSLEQR EAKTNEIKKE 
       610        620        630        640        650
FVSDFETLLL PSGVQEFIMS SELYNSQIES KITYKRSRDM CEISRLVEVP 
       660        670        680        690        700
QGVNPPSPLD AFRSTQQWDV MRCSLRDIII GSERLKEDSS SIYAKILENF 
       710        720        730        740        750
RTLEMFSLFY NYYFAITPLE REIAYKILNE RDWKVSKDGT MWFLRQGEVK 
       760        770        780        790        800
FFNEICEVGD YKIFKLDDWT VIDKINFRLD YSFLQPPVDT ASEVRDVSVD 
       810        820        830 
NNNVNDQSNV TLEQQKQEIS HGKQLLKQLK QGKISV

Length:836

Mass (Da):94,403

Last modified:February 1, 1995 - v2

Checksum:ⁱ69B76694FCC6846F

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	106	I → N in CAA86913 (PubMed:3018676).Curated	1
Sequence conflictⁱ	593	K → R in AAT92916 (PubMed:17322287).Curated	1
Sequence conflictⁱ	725	Y → C in CAA86913 (PubMed:3018676).Curated	1
Sequence conflictⁱ	827 – 836	KQLKQGKISV → ETIETGKN in CAA86913 (PubMed:3018676).Curated	10

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z46861 Genomic DNA Translation: CAA86913.1 X04289 Genomic DNA Translation: CAA27837.1 AY692897 Genomic DNA Translation: AAT92916.1 BK006942 Genomic DNA Translation: DAA08510.1
PIRⁱ	S49940
NCBI Reference Sequences More...RefSeqⁱ	NP_012226.3, NM_001179388.3

Genome annotation databases

EnsemblFungiⁱ	YIL038C; YIL038C; YIL038C
GeneIDⁱ	854773
KEGGⁱ	sce:YIL038C

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P06102	Not3p		YEASC		836	UniRef100_P06102
UPI00076287C1		MARMA		474

Protein	Similar proteins		Species	Score	Length	Source
P06102	Not3p		YEASC		836	UniRef90_P06102
Not3p		YEASV		842
CCR4 transcriptional complex component		YEAS1		836
K7_Not3p		YEASK		836
Not3p		YEAS8		836
+5

Protein	Similar proteins		Species	Score	Length	Source
P06102	Not3p		YEASC		836	UniRef50_P06102
Not3p		YEASV		842
K7_Not3p		YEASK		836
Not3p		YEAS8		836
Not3p		YEAS2		836
+14

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z46861 Genomic DNA Translation: CAA86913.1 X04289 Genomic DNA Translation: CAA27837.1 AY692897 Genomic DNA Translation: AAT92916.1 BK006942 Genomic DNA Translation: DAA08510.1
PIRⁱ	S49940
RefSeqⁱ	NP_012226.3, NM_001179388.3

3D structure databases

ProteinModelPortalⁱ	P06102
SMRⁱ	P06102
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	34952, 249 interactors
ComplexPortalⁱ	CPX-1800 Ccr4-Not complex
DIPⁱ	DIP-2256N
IntActⁱ	P06102, 45 interactors
MINTⁱ	P06102
STRINGⁱ	4932.YIL038C

PTM databases

iPTMnetⁱ	P06102

iPTMnetⁱ

P06102

Proteomic databases

MaxQBⁱ	P06102
PaxDbⁱ	P06102
PRIDEⁱ	P06102
TopDownProteomicsⁱ	P06102

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YIL038C; YIL038C; YIL038C
GeneIDⁱ	854773
KEGGⁱ	sce:YIL038C

Organism-specific databases

EuPathDBⁱ	FungiDB:YIL038C
SGDⁱ	S000001300 NOT3

Phylogenomic databases

GeneTreeⁱ	ENSGT00390000014743
HOGENOMⁱ	HOG000113831
InParanoidⁱ	P06102
KOⁱ	K12580
OMAⁱ	DYQVFDL
OrthoDBⁱ	EOG092C3ROC

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-31310-MONOMER

BioCycⁱ

YEAST:G3O-31310-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P06102

PROⁱ

PR:P06102

Family and domain databases

Gene3Dⁱ	2.30.30.1020, 1 hit
InterProⁱ	View protein in InterPro IPR038635 CCR4-NOT_su2/3/5_N_sf IPR012270 CCR4-NOT_su3/5 IPR007282 NOT IPR007207 Not_N
Pfamⁱ	View protein in Pfam PF04153 NOT2_3_5, 1 hit PF04065 Not3, 1 hit
PIRSFⁱ	PIRSF005290 NOT_su_3_5, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NOT3_YEAST
Accessionⁱ	P06102Primary (citable) accession number: P06102 Secondary accession number(s): D6VVP4, Q6B233
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
	Last sequence update:	February 1, 1995
	Last modified:	October 10, 2018
	This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome IX
Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

UniProtKB

UniParc

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UniProtKB - P06102 (NOT3_YEAST)

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General negative regulator of transcription subunit 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Caution

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ