UniProtKB - P06102 (NOT3_YEAST)
General negative regulator of transcription subunit 3
NOT3
Functioni
Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.
1 PublicationMiscellaneous
Caution
GO - Biological processi
- deadenylation-dependent decapping of nuclear-transcribed mRNA Source: SGD
- nuclear-transcribed mRNA poly(A) tail shortening Source: SGD
- positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
- protein ubiquitination Source: SGD
Keywordsi
Molecular function | Activator, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: General negative regulator of transcription subunit 3 |
Gene namesi | Name:NOT3 Ordered Locus Names:YIL038C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001300, NOT3 |
VEuPathDBi | FungiDB:YIL038C |
Subcellular locationi
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- CCR4-NOT core complex Source: SGD
- cytoplasm Source: SGD
- P-body Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198337 | 1 – 836 | General negative regulator of transcription subunit 3Add BLAST | 836 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 303 | PhosphoserineCombined sources | 1 | |
Modified residuei | 307 | PhosphoserineCombined sources | 1 | |
Modified residuei | 322 | PhosphoserineCombined sources | 1 | |
Modified residuei | 446 | PhosphoserineCombined sources | 1 | |
Modified residuei | 450 | PhosphoserineCombined sources | 1 | |
Cross-linki | 535 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Modified residuei | 565 | PhosphoserineCombined sources | 1 | |
Modified residuei | 569 | PhosphoserineCombined sources | 1 | |
Modified residuei | 571 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 657 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P06102 |
PaxDbi | P06102 |
PRIDEi | P06102 |
TopDownProteomicsi | P06102 |
PTM databases
iPTMneti | P06102 |
Interactioni
Subunit structurei
Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.
2 PublicationsBinary interactionsi
P06102
With | #Exp. | IntAct |
---|---|---|
CAF40 [P53829] | 3 | EBI-12165,EBI-28306 |
CDC36 [P06100] | 2 | EBI-12165,EBI-12153 |
CDC39 [P25655] | 3 | EBI-12165,EBI-12139 |
Protein-protein interaction databases
BioGRIDi | 34952, 256 interactors |
ComplexPortali | CPX-1800, CCR4-NOT mRNA deadenylase complex |
DIPi | DIP-2256N |
IntActi | P06102, 45 interactors |
MINTi | P06102 |
STRINGi | 4932.YIL038C |
Miscellaneous databases
RNActi | P06102, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 252 – 284 | DisorderedSequence analysisAdd BLAST | 33 | |
Regioni | 296 – 391 | DisorderedSequence analysisAdd BLAST | 96 | |
Regioni | 410 – 471 | DisorderedSequence analysisAdd BLAST | 62 | |
Regioni | 513 – 532 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 537 – 583 | DisorderedSequence analysisAdd BLAST | 47 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 36 – 68 | Sequence analysisAdd BLAST | 33 | |
Coiled coili | 119 – 195 | Sequence analysisAdd BLAST | 77 | |
Coiled coili | 255 – 292 | Sequence analysisAdd BLAST | 38 | |
Coiled coili | 803 – 831 | Sequence analysisAdd BLAST | 29 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 265 – 284 | Basic and acidic residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 341 – 387 | Polar residuesSequence analysisAdd BLAST | 47 | |
Compositional biasi | 414 – 471 | Polar residuesSequence analysisAdd BLAST | 58 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2150, Eukaryota |
HOGENOMi | CLU_020483_0_0_1 |
InParanoidi | P06102 |
OMAi | IKTWAAG |
Family and domain databases
Gene3Di | 2.30.30.1020, 1 hit |
InterProi | View protein in InterPro IPR038635, CCR4-NOT_su2/3/5_N_sf IPR012270, CCR4-NOT_su3/5 IPR040168, Not2/3/5 IPR007282, NOT2/3/5_C IPR007207, Not_N |
PANTHERi | PTHR23326, PTHR23326, 1 hit |
Pfami | View protein in Pfam PF04153, NOT2_3_5, 1 hit PF04065, Not3, 1 hit |
PIRSFi | PIRSF005290, NOT_su_3_5, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAHRKLQQEV DRVFKKINEG LEIFNSYYER HESCTNNPSQ KDKLESDLKR
60 70 80 90 100
EVKKLQRLRE QIKSWQSSPD IKDKDSLLDY RRSVEIAMEK YKAVEKASKE
110 120 130 140 150
KAYSNISLKK SETLDPQERE RRDISEYLSQ MIDELERQYD SLQVEIDKLL
160 170 180 190 200
LLNKKKKTSS TTNDEKKEQY KRFQARYRWH QQQMELALRL LANEELDPQD
210 220 230 240 250
VKNVQDDINY FVESNQDPDF VEDETIYDGL NLQSNEAIAH EVAQYFASQN
260 270 280 290 300
AEDNNTSDAN ESLQDISKLS KKEQRKLERE AKKAAKLAAK NATGAAIPVA
310 320 330 340 350
GPSSTPSPVI PVADASKETE RSPSSSPIHN ATKPEEAVKT SIKSPRSSAD
360 370 380 390 400
NLLPSLQKSP SSATPETPTN VHTHIHQTPN GITGATTLKP ATLPAKPAGE
410 420 430 440 450
LKWAVAASQA VEKDRKVTSA SSTISNTSTK TPTTAAATTT SSNANSRIGS
460 470 480 490 500
ALNTPKLSTS SLSLQPDNTG ASSSAATAAA VLAAGAAAVH QNNQAFYRNM
510 520 530 540 550
SSSHHPLVSL ATNPKSEHEV ATTVNQNGPE NTTKKVMEQK EEESPEERNK
560 570 580 590 600
LQVPTFGVFD DDFESDRDSE TEPEEEEQPS TPKYLSLEQR EAKTNEIKKE
610 620 630 640 650
FVSDFETLLL PSGVQEFIMS SELYNSQIES KITYKRSRDM CEISRLVEVP
660 670 680 690 700
QGVNPPSPLD AFRSTQQWDV MRCSLRDIII GSERLKEDSS SIYAKILENF
710 720 730 740 750
RTLEMFSLFY NYYFAITPLE REIAYKILNE RDWKVSKDGT MWFLRQGEVK
760 770 780 790 800
FFNEICEVGD YKIFKLDDWT VIDKINFRLD YSFLQPPVDT ASEVRDVSVD
810 820 830
NNNVNDQSNV TLEQQKQEIS HGKQLLKQLK QGKISV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 106 | I → N in CAA86913 (PubMed:3018676).Curated | 1 | |
Sequence conflicti | 593 | K → R in AAT92916 (PubMed:17322287).Curated | 1 | |
Sequence conflicti | 725 | Y → C in CAA86913 (PubMed:3018676).Curated | 1 | |
Sequence conflicti | 827 – 836 | KQLKQGKISV → ETIETGKN in CAA86913 (PubMed:3018676).Curated | 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46861 Genomic DNA Translation: CAA86913.1 X04289 Genomic DNA Translation: CAA27837.1 AY692897 Genomic DNA Translation: AAT92916.1 BK006942 Genomic DNA Translation: DAA08510.1 |
PIRi | S49940 |
RefSeqi | NP_012226.3, NM_001179388.3 |
Genome annotation databases
EnsemblFungii | YIL038C_mRNA; YIL038C; YIL038C |
GeneIDi | 854773 |
KEGGi | sce:YIL038C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46861 Genomic DNA Translation: CAA86913.1 X04289 Genomic DNA Translation: CAA27837.1 AY692897 Genomic DNA Translation: AAT92916.1 BK006942 Genomic DNA Translation: DAA08510.1 |
PIRi | S49940 |
RefSeqi | NP_012226.3, NM_001179388.3 |
3D structure databases
SMRi | P06102 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34952, 256 interactors |
ComplexPortali | CPX-1800, CCR4-NOT mRNA deadenylase complex |
DIPi | DIP-2256N |
IntActi | P06102, 45 interactors |
MINTi | P06102 |
STRINGi | 4932.YIL038C |
PTM databases
iPTMneti | P06102 |
Proteomic databases
MaxQBi | P06102 |
PaxDbi | P06102 |
PRIDEi | P06102 |
TopDownProteomicsi | P06102 |
Genome annotation databases
EnsemblFungii | YIL038C_mRNA; YIL038C; YIL038C |
GeneIDi | 854773 |
KEGGi | sce:YIL038C |
Organism-specific databases
SGDi | S000001300, NOT3 |
VEuPathDBi | FungiDB:YIL038C |
Phylogenomic databases
eggNOGi | KOG2150, Eukaryota |
HOGENOMi | CLU_020483_0_0_1 |
InParanoidi | P06102 |
OMAi | IKTWAAG |
Miscellaneous databases
PROi | PR:P06102 |
RNActi | P06102, protein |
Family and domain databases
Gene3Di | 2.30.30.1020, 1 hit |
InterProi | View protein in InterPro IPR038635, CCR4-NOT_su2/3/5_N_sf IPR012270, CCR4-NOT_su3/5 IPR040168, Not2/3/5 IPR007282, NOT2/3/5_C IPR007207, Not_N |
PANTHERi | PTHR23326, PTHR23326, 1 hit |
Pfami | View protein in Pfam PF04153, NOT2_3_5, 1 hit PF04065, Not3, 1 hit |
PIRSFi | PIRSF005290, NOT_su_3_5, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NOT3_YEAST | |
Accessioni | P06102Primary (citable) accession number: P06102 Secondary accession number(s): D6VVP4, Q6B233 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | February 1, 1995 | |
Last modified: | September 29, 2021 | |
This is version 198 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IX
Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names - SIMILARITY comments
Index of protein domains and families