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Protein

General negative regulator of transcription subunit 2

Gene

CDC36

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. NOT2 is required for the integrity of the complex. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

Miscellaneous

Present with 2120 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

Keywordsi

Molecular functionActivator, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29557-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
General negative regulator of transcription subunit 2
Alternative name(s):
cell division cycle protein 36
Gene namesi
Name:CDC36
Synonyms:DNA19, NOT2
Ordered Locus Names:YDL165W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL165W
SGDiS000002324 CDC36

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001983361 – 191General negative regulator of transcription subunit 2Add BLAST191

Proteomic databases

MaxQBiP06100
PaxDbiP06100
PRIDEiP06100

Interactioni

Subunit structurei

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1 and NOT5. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi31900, 230 interactors
ComplexPortaliCPX-1800 Ccr4-Not complex
DIPiDIP-590N
IntActiP06100, 24 interactors
MINTiP06100
STRINGi4932.YDL165W

Structurei

Secondary structure

1191
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP06100
SMRiP06100
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000001285
HOGENOMiHOG000246523
InParanoidiP06100
KOiK12605
OMAiVARNWRY
OrthoDBiEOG092C2LUV

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR007282 NOT
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit

Sequencei

Sequence statusi: Complete.

P06100-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEKFGLKALV PLLKLEDKEL SSTYDHSMTL GADLSSMLYS LGIPRDSQDH
60 70 80 90 100
RVLDTFQSPW AETSRSEVEP RFFTPESFTN IPGVLQSTVT PPCFNSIQND
110 120 130 140 150
QQRVALFQDE TLFFLFYKHP GTVIQELTYL ELRKRNWRYH KTLKAWLTKD
160 170 180 190
PMMEPIVSAD GLSERGSYVF FDPQRWEKCQ RDFLLFYNAI M
Length:191
Mass (Da):22,363
Last modified:January 1, 1988 - v1
Checksum:i7D0B69657F791371
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04287 Genomic DNA Translation: CAA27835.1
X03246 Genomic DNA Translation: CAA27006.1
Z67750 Genomic DNA Translation: CAA91581.1
Z74213 Genomic DNA Translation: CAA98739.1
X68020 Genomic DNA Translation: CAA48160.1
AY557644 Genomic DNA Translation: AAS55970.1
BK006938 Genomic DNA Translation: DAA11696.1
PIRiS12304 RGBY36
RefSeqiNP_010116.1, NM_001180225.1

Genome annotation databases

EnsemblFungiiYDL165W; YDL165W; YDL165W
GeneIDi851389
KEGGisce:YDL165W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04287 Genomic DNA Translation: CAA27835.1
X03246 Genomic DNA Translation: CAA27006.1
Z67750 Genomic DNA Translation: CAA91581.1
Z74213 Genomic DNA Translation: CAA98739.1
X68020 Genomic DNA Translation: CAA48160.1
AY557644 Genomic DNA Translation: AAS55970.1
BK006938 Genomic DNA Translation: DAA11696.1
PIRiS12304 RGBY36
RefSeqiNP_010116.1, NM_001180225.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BY6X-ray2.80B/E1-191[»]
ProteinModelPortaliP06100
SMRiP06100
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31900, 230 interactors
ComplexPortaliCPX-1800 Ccr4-Not complex
DIPiDIP-590N
IntActiP06100, 24 interactors
MINTiP06100
STRINGi4932.YDL165W

Proteomic databases

MaxQBiP06100
PaxDbiP06100
PRIDEiP06100

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL165W; YDL165W; YDL165W
GeneIDi851389
KEGGisce:YDL165W

Organism-specific databases

EuPathDBiFungiDB:YDL165W
SGDiS000002324 CDC36

Phylogenomic databases

GeneTreeiENSGT00390000001285
HOGENOMiHOG000246523
InParanoidiP06100
KOiK12605
OMAiVARNWRY
OrthoDBiEOG092C2LUV

Enzyme and pathway databases

BioCyciYEAST:G3O-29557-MONOMER

Miscellaneous databases

PROiPR:P06100

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR007282 NOT
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNOT2_YEAST
AccessioniPrimary (citable) accession number: P06100
Secondary accession number(s): D6VRI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 20, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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Main funding by: National Institutes of Health

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