UniProtKB - P05986 (KAPC_YEAST)
Protein
cAMP-dependent protein kinase type 3
Gene
TPK3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 1590 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Activity regulationi
Activated by cAMP.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 117 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 211 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 94 – 102 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- AMP-activated protein kinase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- cAMP-dependent protein kinase activity Source: SGD
- protein kinase activity Source: SGD
- protein serine kinase activity Source: RHEA
- protein threonine kinase activity Source: RHEA
GO - Biological processi
- mitochondrion organization Source: SGD
- negative regulation of cytoplasmic mRNA processing body assembly Source: SGD
- negative regulation of cytoplasmic translation Source: SGD
- protein kinase A signaling Source: SGD
- protein phosphorylation Source: SGD
- Ras protein signal transduction Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, cAMP, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | YEAST:G3O-31934-MONOMER |
BRENDAi | 2.7.11.11, 984 |
Reactomei | R-SCE-163615, PKA activation R-SCE-164378, PKA activation in glucagon signalling R-SCE-180024, DARPP-32 events R-SCE-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-SCE-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-SCE-5610787, Hedgehog 'off' state R-SCE-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:TPK3 Synonyms:PKA3 Ordered Locus Names:YKL166C ORF Names:YKL630 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001649, TPK3 |
VEuPathDBi | FungiDB:YKL166C |
Subcellular locationi
Nucleus
- nucleus Source: SGD
Other locations
- cAMP-dependent protein kinase complex Source: SGD
- cytoplasm Source: SGD
- cytoplasmic stress granule Source: SGD
- P-body Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086071 | 1 – 398 | cAMP-dependent protein kinase type 3Add BLAST | 398 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 15 | PhosphoserineCombined sources | 1 | |
Modified residuei | 55 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P05986 |
PaxDbi | P05986 |
PRIDEi | P05986 |
PTM databases
iPTMneti | P05986 |
Interactioni
Binary interactionsi
P05986
With | #Exp. | IntAct |
---|---|---|
BCY1 [P07278] | 4 | EBI-9470,EBI-9475 |
Protein-protein interaction databases
BioGRIDi | 33971, 321 interactors |
ComplexPortali | CPX-571, cAMP-dependent protein kinase complex variant 3 CPX-573, cAMP-dependent protein kinase complex variant 5 CPX-574, cAMP-dependent protein kinase complex variant 6 |
DIPi | DIP-550N |
IntActi | P05986, 28 interactors |
MINTi | P05986 |
STRINGi | 4932.YKL166C |
Miscellaneous databases
RNActi | P05986, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 88 – 342 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 255 | |
Domaini | 343 – 398 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 56 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0616, Eukaryota |
GeneTreei | ENSGT00940000176357 |
HOGENOMi | CLU_000288_63_5_1 |
InParanoidi | P05986 |
OMAi | TCLEDFD |
Family and domain databases
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P05986-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYVDPMNNNE IRKLSITAKT ETTPDNVGQD IPVNAHSVHE ECSSNTPVEI
60 70 80 90 100
NGRNSGKLKE EASAGICLVK KPMLQYRDTS GKYSLSDFQI LRTLGTGSFG
110 120 130 140 150
RVHLIRSNHN GRFYALKTLK KHTIVKLKQV EHTNDERRML SIVSHPFIIR
160 170 180 190 200
MWGTFQDSQQ VFMVMDYIEG GELFSLLRKS QRFPNPVAKF YAAEVCLALE
210 220 230 240 250
YLHSKDIIYR DLKPENILLD KNGHIKITDF GFAKYVPDVT YTLCGTPDYI
260 270 280 290 300
APEVVSTKPY NKSVDWWSFG VLIYEMLAGY TPFYNSNTMK TYENILNAEL
310 320 330 340 350
KFPPFFHPDA QDLLKKLITR DLSERLGNLQ NGSEDVKNHP WFNEVIWEKL
360 370 380 390
LARYIETPYE PPIQQGQGDT SQFDRYPEEE FNYGIQGEDP YMDLMKEF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 | D → E in AAA35166 (PubMed:3036373).Curated | 1 | |
Sequence conflicti | 208 | I → T in AAA35166 (PubMed:3036373).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17074 Genomic DNA Translation: AAA35166.1 Z26878 Genomic DNA Translation: CAA81521.1 Z28166 Genomic DNA Translation: CAA82008.1 M81697 Genomic DNA Translation: AAA34791.1 BK006944 Genomic DNA Translation: DAA08999.1 |
PIRi | S37996, OKBYC3 |
RefSeqi | NP_012755.1, NM_001179732.1 |
Genome annotation databases
EnsemblFungii | YKL166C_mRNA; YKL166C; YKL166C |
GeneIDi | 853688 |
KEGGi | sce:YKL166C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17074 Genomic DNA Translation: AAA35166.1 Z26878 Genomic DNA Translation: CAA81521.1 Z28166 Genomic DNA Translation: CAA82008.1 M81697 Genomic DNA Translation: AAA34791.1 BK006944 Genomic DNA Translation: DAA08999.1 |
PIRi | S37996, OKBYC3 |
RefSeqi | NP_012755.1, NM_001179732.1 |
3D structure databases
SMRi | P05986 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33971, 321 interactors |
ComplexPortali | CPX-571, cAMP-dependent protein kinase complex variant 3 CPX-573, cAMP-dependent protein kinase complex variant 5 CPX-574, cAMP-dependent protein kinase complex variant 6 |
DIPi | DIP-550N |
IntActi | P05986, 28 interactors |
MINTi | P05986 |
STRINGi | 4932.YKL166C |
PTM databases
iPTMneti | P05986 |
Proteomic databases
MaxQBi | P05986 |
PaxDbi | P05986 |
PRIDEi | P05986 |
Genome annotation databases
EnsemblFungii | YKL166C_mRNA; YKL166C; YKL166C |
GeneIDi | 853688 |
KEGGi | sce:YKL166C |
Organism-specific databases
SGDi | S000001649, TPK3 |
VEuPathDBi | FungiDB:YKL166C |
Phylogenomic databases
eggNOGi | KOG0616, Eukaryota |
GeneTreei | ENSGT00940000176357 |
HOGENOMi | CLU_000288_63_5_1 |
InParanoidi | P05986 |
OMAi | TCLEDFD |
Enzyme and pathway databases
BioCyci | YEAST:G3O-31934-MONOMER |
BRENDAi | 2.7.11.11, 984 |
Reactomei | R-SCE-163615, PKA activation R-SCE-164378, PKA activation in glucagon signalling R-SCE-180024, DARPP-32 events R-SCE-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-SCE-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-SCE-5610787, Hedgehog 'off' state R-SCE-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
PROi | PR:P05986 |
RNActi | P05986, protein |
Family and domain databases
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KAPC_YEAST | |
Accessioni | P05986Primary (citable) accession number: P05986 Secondary accession number(s): D6VX33 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1988 |
Last sequence update: | June 1, 1994 | |
Last modified: | April 7, 2021 | |
This is version 205 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - SIMILARITY comments
Index of protein domains and families